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Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

Microsystems & Nanoengineering 2017年第1期3卷 260-266页

作者： Samuel C.Kim Gayatri Premasekharan Iain C.Clark hawi b.gemeda Pamela L.Paris Adam R.Abate Department of bioengineering and Therapeutic Sciences University of CaliforniaSan Francisco(UCSF)California Institute for Quantitative Biosciences(QB3)San FranciscoSan FranciscoCA 94158USA Department of Urology Division of Hematology&OncologyUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94158USA

Uniform amplification of low-input DNA is important for applications across biology,including single-cell genomics,forensic science,and microbial and viral ***,the requisite biochemical amplification methods are prone to bias,skewing sequence proportions and obscuring signals relating to copy *** droplet multiple displacement amplification enables uniform amplification but requires expert knowledge of microfluidics to generate monodisperse *** addition,existing microfluidic methods are tedious and labor intensive for preparing many ***,we introduce rapid-emulsification multiple displacement amplification,a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet *** conventional microfluidic devices require >10 min to emulsify a sample,our system requires tens of seconds and yields data of equivalent *** demonstrate the approach by using it to accurately measure copy number variation(CNV)in single cancer cells.

关键词： amplification bias copy number variation ddMDA droplet microfluidics multiple displacement amplification

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Experimental investigation and optimization of the gasification parameters of macadamia nutshells in a batch-fed bubbling fluidized bed gasifier with air preheating

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Energy Storage and Saving 2023年第4期2卷 559-570页

作者： Fredrick Irungu Njuguna Hiram M.Ndiritu benson b.Gathitu Meshack hawi Jotham Muthoka Munyalo School of Mechanical Manufacturing and Materials EngineeringJomo Kenyatta University of Agriculture and TechnologyNairobi62000-00200Kenya School of biosystems and Environmental Engineering Jomo Kenyatta University of Agriculture and TechnologyNairobi62000-00200Kenya

Gasification of biomass waste has a significant potential to reduce environmental impact and promote sustainability by producing syngas,which is considered as renewable *** work investigated the gasification of macadamia nutshells in air-preheated,batch-fed fluidized bed *** study conducted a parametric analysis to assess the effect of equivalence ratio(ER)and air temperature on the gasifier temperature profile and its performance based on gas composition,higher heating value(HHV),and gas *** research was conducted within the range of 0.15-0.35 for the ER and 25-825℃ for the air ***-objective numerical optimization was conducted using response surface methodology(RSM).From the parametric study,a distinct temperature profile was observed along the gasifier height,with the peak temperature near the top of the fluidized bed section and the lowest temperature at the top of the *** preheating mostly favored gasification temperature at the lower part of the gasifier and showed rare significance at the *** improvement in gasifier performance was observed beyond an air temperature of 620℃,which was identified as the ideal air-preheating *** of variance(ANOVA)revealed that the ER was the most influential parameter in the production of combustible gasses,syngas HHV and gas *** preheating did not have a significant effect on methane production and gas *** most optimal values for ER and air temperature were obtained as 0.195℃ and 620℃,respectively,producing optimal values of 9.54,14.65%,2.03,4.02 MJ⋅Nm^(-3),and 1.82 Nm^(3)⋅kg^(-1) for hydrogen,carbon monoxide,methane,HHV,and gas yield,respectively.

关键词： biomass gasification Air preheating Optimization Response surface methodology

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