检索结果-南通市图书馆

生物化学杂志 1992年第6期8卷 676-679页

作者：贾弘禔 J.schmidt 北京医科大学生物化学教研室 Dept.of Biochemistry and Cell Biology SUNY at Stony BrookNY11794

本工作利用瞬时表达研究了鸡Ach Rγ亚基基因5′连接区的调控机能。CAt分析表明,γ基因5′连接区-509/-302和-324/+36序列均可独立激活SV40启动子的转录,这种激活作用具有组织特异性,与方向、距离无关,符合增强子样作用。

关键词：乙酰胆碱受体 γ亚基基因

Observation of a single protein by ultrafast X-ray diffraction

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2024年第1期13卷 80-90页

作者： tomas Ekeberg Dameli Assalauova Johan Bielecki Rebecca Boll Benedikt J.Daurer Lutz A.Eichacker Linda E.Franken Davide E.Galli Luca Gelisio Lars Gumprecht Laura H.Gunn Janos Hajdu Robert hartmann Dirk Hasse Alexandr Ignatenko Jayanath Koliyadu Olena Kulyk Ruslan Kurta Markus Kuster Wolfgang Lugmayr Jannik Lübke Adrian P.Mancuso tommaso Mazza Carl Nettelblad Yevheniy Ovcharenko Daniel E.Rivas Max Rose Amit K.Samanta Philipp schmidt Egor Sobolev Nicusor timneanu Sergey Usenko Daniel Westphal tamme Wollweber Lena Worbs Paul Lourdu Xavier Hazem Yousef Kartik Ayyer Henry N.Chapman Jonas A.Sellberg Carolin Seuring Ivan A.Vartanyants Jochen Küpper Michael Meyer Filipe R.N.C.Maia Laboratory of Molecular Biophysics Department of Cell and Molecular BiologyUppsala UniversityHusargatan 3(Box 596)SE-75124 UppsalaSweden Deutsches Electronen-Synchrotron DESY Notkestrasse 8522607 HamburgGermany European XFEL Holzkoppel 422869 SchenefeldGermany Diamond Light Source Harwell Science&Innovation CampusDidcot OX110DEUK University of Stavanger Centre Organelle ResearchRichard-Johnsensgate 44021 StavangerNorway Leibniz Institute for Experimental Virology(HPI) Centre for Structural Systems BiologyNotkestraße 8522607 HamburgGermany Dipartimento di Fisica“Aldo Pontremoli” Universitàdegli Studi di Milanovia Celoria 1620133 MilanoItaly Center for Free-Electron Laser Science DESY22607 HamburgGermany Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853USA PNSensor GmbH Otto-Hahn-Ring 6D-81739 MünchenGermany Biomedical and X-Ray Physics Department of Applied PhysicsAlbaNova University CenterKTH Royal Institute of TechnologySE-10691 StockholmSweden ELI Beamlines/IoP Institute of Physics AS CR v.v.i.Na Slovance 218221 Prague 8Czech Republic Multi-User CryoEM Facility Centre for Structural Systems BiologyNotkestr.8522607 HamburgGermany University Medical Center Hamburg-Eppendorf(UKE) Martinistrasse 5220246 HamburgGermany the Hamburg Center for Ultrafast Imaging Universität HamburgLuruper Chaussee 14922761 HamburgGermany Department of Physics Universität HamburgLuruper Chaussee 14922761 HamburgGermany Department of Chemistry and Physics La Trobe Institute for Molecular ScienceLa Trobe UniversityMelbourneVIC 3086Australia Division of Scientific Computing Science for Life LaboratoryDepartment of Information TechnologyUppsala UniversityBox 337SE-75105 UppsalaSweden European Molecular Biology Laboratory c/o DESYNotkestrasse 8522607 HamburgGermany Department of Physics and Astronomy Uppsala UniversityBox 516SE-75120 UppsalaSweden Max Planck Institute for the Structure and Dynamics of Matter Luruper Chaussee 14922761 HamburgGe

the idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired *** was one of the arguments for building X-ray free-electron *** to theory,the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier,and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably *** was first demonstrated on biological samples a decade ago on the giant *** then,a large collaboration has been pushing the limit of the smallest sample that can be *** ability to capture snapshots on the timescale of atomic vibrations,while keeping the sample at room temperature,may allow probing the entire conformational phase space of *** we show the first observation of an X-ray diffraction pattern from a single protein,that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays,and demonstrate that the concept of diffraction before destruction extends to single *** the pattern,it is possible to determine the approximate orientation of the *** experiment demonstrates the feasibility of ultrafast imaging of single proteins,opening the way to single-molecule time-resolved studies on the femtosecond timescale.

关键词： smallest intense ultrafast

Double stratified radiative flow of an Oldroyd-B nanofluid with nonlinear convection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2019年第12期40卷 1861-1878页

作者： t.HAYAt M.Z.KIYANI I.AHMAD A.ALSAEDI Department of Mathematics Quaid-i-Azam University Nonlinear Analysis and Applied Mathematics Research Group Department of MathematicsFaculty of Science King Abdulaziz University Department of Mathematics University of Azad Jammu & Kashmir

the nonlinear convective flow of an Oldroyd-B fluid due to a nonlinear stretching sheet with varying thickness is examined. the salient features of the random movement and thermophoresis are described. Formulation is made with the nonlinear thermal radiation and heat generation/absorption. Further, the convective conditions and double stratification are taken into account. the resulting flow problems are tackled by the optimal homotopy analysis method(OHAM). the resulting nonlinear problems are solved for the velocity, temperature, and concentration fields. the temperature and concentration gradients are numerically discussed. the total residual error is *** Nusselt number is an increasing function of the radiation parameter. the Sherwood number increases with the increase in the solutal stratification or the schmidt *** main outcomes are presented in conclusions. this study has a wide range of applications such as thermal stratification of oceans, reservoirs, and rivers, density stratification of atmosphere, hydraulic lifts, and polymer processing.

关键词： Oldryod-B fluid nonlinear thermal radiation double stratification nonlinear convective flow

Investigation of generalized Fick's and Fourier's laws in the second-grade fluid flow

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2018年第11期39卷 1617-1630页

作者： t.HAYAt S.AHMAD M.I.KHAN A.ALSAEDI Department of Mathematics Quaid-i-Azam University Nonlinear Analysis and Applied Mathematics (NAAM) Research Group Department of Mathematics Faculty of Science King Abdulaziz University

the second-grade fluid flow due to a rotating porous stretchable disk is modeled and analyzed. A porous medium is characterized by the Darcy relation. the heat and mass transport are characterized through Cattaneo-Christov double *** thermal and solutal stratifications at the surface are also accounted. the relevant nonlinear ordinary differential systems after using appropriate transformations are solved for the solutions with the homotopy analysis method(HAM). the effects of various involved variables on the temperature, velocity, concentration, skin friction, mass transfer rate, and heat transfer rate are discussed through graphs. From the obtained results,decreasing tendencies for the radial, axial, and tangential velocities are observed. temperature is a decreasing function of the Reynolds number, thermal relaxation parameter,and Prandtl number. Moreover, the mass diffusivity decreases with the schmidt number.

关键词： second-grade liquid thermal and solutal stratification Cattaneo-Christov double diffusion rotating stretchable disk

Estimation of Fatigue Life of Laser Welded AISI304 Stainless Steel t-Joint Based on Experiments and Recommendations in Design Codes

在线全文

学校读者我要写书评

暂无评论

World Journal of Mechanics 2013年第3期3卷 178-183页

作者： S?ren Heide Lambertsen Lars Damkilde Anders schmidt Kristensen Ronnie Refstrup Pedersen Division of Structures and Materials Aalborg University EsbjergEsbjergDenmark

In this paper the fatigue behavior of laser welded t-joints of stainless steel AISI304 is investigated experimentally. In the fatigue experiments 36 specimens with a sheet thickness of 1 mm are exposed to one-dimensional cyclic loading. three different types of specimens are adopted. three groups of specimens are used, two of these are non-welded and the third is welded with a transverse welding (t-Joint). the 13 laser welded specimens are cut out with a milling cutter. the non-welded specimens are divided in 13 specimens cut out with a milling cutter and 10 specimens cut out by a plasma cutter. the non-welded specimens are used to study the influence of heat and surface effects on the fatigue life. the fatigue life from the experiments is compared to fatigue life calculated from the guidelines in the standards DNV-RP-C203 and EUROCODE 3 EN-1993-1-9. Insignificant differences in fatigue life of the welded and non-welded specimens are observed in the experiments and the largest difference is found in the High Cycle Fatigue (HCF) area. the specimens show a lower fatigue life compared to DNV-RP-C203 and EUROCODE 3 EN-1993-1-9 when the specimens are exposed to less than 4.0 1E06 cycles. therefore, we conclude that the fatigue life assessment according to the mentioned standards is not satisfactory and reliable.

关键词： AISI304 Laser Welding Austenite Martensite

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

INS／GPS技术趋势

战术导弹控制技术 2009年第2期16卷 54-64页

作者： George t. schmidt 李文耀（译）尚克军（译）刘峰（译）林思刚（校译）马萨诸塞州技术研究所北京自动化控制技术研究所北京100074

集中讨论了惯性器件、GPS、INS／GPS组合系统的精度、技术发展趋势以及对干扰的一些思考，这些技术将促成精度优于1m的导航系统。在惯性器件方面，介绍了主流的惯性器件技术，预测了未来的惯性器件及惯性导航系统的发展，阐述了计划中的... 详细信息

集中讨论了惯性器件、GPS、INS／GPS组合系统的精度、技术发展趋势以及对干扰的一些思考，这些技术将促成精度优于1m的导航系统。在惯性器件方面，介绍了主流的惯性器件技术，预测了未来的惯性器件及惯性导航系统的发展，阐述了计划中的GPS精度增强措施，描述了INS／GPS深组合的趋势，探讨了其组合互补优势，枚举了几个干扰影响的实例并介绍了有望提高系统鲁棒性的一些技术的发展趋势。

关键词： INS/GPS GPS技术技术发展趋势惯性导航系统 GPS精度惯性器件组合系统干扰影响

Spatial and spectral mode mapping of a dielectric nanodot by broadband interferometric homodyne scanning near-field spectroscopy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advanced Photonics 2020年第4期2卷 55-65页

作者： Jinxin Zhan Wei Wang Jens Brauer Lukas schmidt-Mende Christoph Lienau Petra Groß Carl von Ossietzky Universität Institut für Physik and Center of Interface ScienceOldenburgGermany University of Konstanz Department of PhysicsKonstanzGermany

We investigate the optical properties of nanostructures of antimony sulfide(Sb2S3),a direct-bandgap semiconductor material that has recently sparked considerable interest as a thin film solar cell *** from a nanoparticle ink solution and two-and three-dimensional nanostructuring with pattern sizes down to 50 nm have recently been *** into the yet unknown nanoscopic optical properties of these nanostructures is highly desired for their future applications in *** implement a spectrally broadband scattering-type near-field optical spectroscopy technique to study individual Sb2S3 nanodots with a 20-nm spatial resolution,covering the range from 700 to 900 *** show that in this below-bandgap range,the Sb2S3 nanostructures act as high-refractive-index,low-loss waveguides with mode profiles close to those of idealized cylindrical waveguides,despite a considerable structural *** combination with their high above-bandgap absorption,this makes them promising candidates for applications as dielectric metamaterials,specifically for ultrafast photoswitching.

关键词： near-field spectroscopy scattering-type near-field optical microscopy tip-modulation SNOM dielectric nanostructure thin film patterning

在线全文

学校读者我要写书评

暂无评论

慢性紧张型头痛患者的灰质萎缩

世界核心医学期刊文摘（神经病学分册） 2006年第3期2卷 31-32页

作者： schmidt-Wilcke t. Leinisch E. Straube A. A. May 陈海 Department of Systems Neuroscience Universitts-Krankenhaus Eppendorf (UKE) Martinistr. 52 D-20246 Hamburg Germany

Using MRI and voxel-based morphometry, the authors investigated 20 patients with chronic tension type headache (CttH) and 20 patients with medication-overus e headache and compared them to 40 controls with no headache history. Only patie nts with CttH demonstrated a significant gray matter decrease in regions known t o be involved in pain processing. the finding implies that the alterations are s pecific to CttH rather than a response to chronic head pain or chronification pe r se.

关键词：慢性紧张型头痛头痛患者慢性头痛形态分析法慢性化

成功置入药物洗脱支架后血栓形成的发生率、预测因素和预后

在线全文

学校读者我要写书评

暂无评论

世界核心医学期刊文摘（心脏病学分册） 2005年第10期1卷 25-26页

作者： Iakovou I. schmidt t. Bonizzoni E. A. Colombo 任付先 EMO Centro Cuore Columbus 48 Via M Buonarroti 20145 Milan Italy

traditionally, stent thrombosis has been regarded as a complication of percutaneous coronary interventions during the first 30 postprocedural days. However, delayed endothelialization associated with the implantation of drug-eluting stents may extend the risk of thrombosis beyond 30 days. Data are limited regarding the risks and the impact of this phenomenon outside clinical trials. Abstract: to evaluate the incidence, predictors, and clinical outcome of stent thrombosis after implantation of sirolimus-eluting and paclitaxel-eluting stents in routine clinical practice. Design, Setting, and Patients: Prospective observational cohort study conducted at 1 academic hospital and 2 community hospitals in Germany and Italy. A total of 2229 consecutive patients underwent successful implantation of sirolimus-eluting(1062 patients, 1996 lesions, 2272 stents) or paclitaxel-eluting(1167 patients, 1801 lesions, 2223 stents) stents between April 2002 and January 2004. Interventions: Implantation of a drug-eluting stent(sirolimus or paclitaxel). All patients were pretreated with ticlopidine or clopidogrel and aspirin. Aspirin was continued indefinitely and clopidogrel or ticlopidine for at least 3 months after sirolimus-eluting and for at least 6 months after paclitaxeleluting stent implantation. Main Outcome Measures: Subacute thrombosis(from procedure end through 30 days), late thrombosis( >30 days), and cumulative stent thrombosis. Results: At 9- month follow-up, 29 patients(1.3% ) had stent thrombosis(9[0.8% ] with sirolimus and 20[1.7% ] with paclitaxel; P=.09). Fourteen patients had subacute thrombosis(0.6% ) and 15 patients had late thrombosis(0.7% ). Among these 29 patients, 13 died(case fatality rate, 45% ). Independent predictors of stent thrombosis were premature antiplatelet therapy discontinuation(hazard ratio[HR], 89.78; 95% CI, 29.90- 269.60; Ption lesions(HR, 6.42; 95% CI, 2.93- 14.07; P< .001),

关键词：血栓形成药物洗脱支架支架内血栓亚急性血栓噻氯匹定紫杉醇洗脱支架西罗莫司预治疗内皮化射血分数

在线全文

学校读者我要写书评

暂无评论

顺利运送乘客和货物

现代制造 2016年第20期 36-37页

作者： PEtER schmidt

无论是从法兰克福出发还是从阿姆斯特丹出发：国际航空枢纽站一天24h总是呈现出一派紧张繁忙的工作景象。在这里，客机或是货机以分钟为节拍起飞，飞往世界各地。2014年全年，仅从法兰克福机场飞往美国、亚洲、非洲、澳大利亚和欧洲等... 详细信息

无论是从法兰克福出发还是从阿姆斯特丹出发：国际航空枢纽站一天24h总是呈现出一派紧张繁忙的工作景象。在这里，客机或是货机以分钟为节拍起飞，飞往世界各地。2014年全年，仅从法兰克福机场飞往美国、亚洲、非洲、澳大利亚和欧洲等国的乘客便达5900万人次，运送的货物达220万t。而乘客几乎注意不到的是，机场有大量的物流设备在保障着机场的正常运行：成千吨的货物要进行装卸，服务车要进行协调，物流设备要准确的抵达飞机起飞所处的位置。

关键词：货物乘客法兰克福机场物流设备飞机起飞阿姆斯特丹国际航空澳大利亚