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Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture

American Journal of Plant Sciences 2019年第7期10卷 1233-1238页

作者： Zuraida Ab Rahman hartinee abbas Ayu Nazreena Othman Wan Zaliha Wan Sembok Biotechnology and Nanotechnology Research Centre Malaysian Agricultural Research and Development Institute (MARDI) Kuala Lumpur Malaysia Horticulture Research Centre Malaysian Agricultural Research and Development Institute (MARDI) Sintok Malaysia School of Food Science and Technology Universiti Malaysia Terengganu Kuala Terengganu Malaysia

The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.

关键词： Pineapple MD2 Micropropagation Liquid-Shake Culture Agitation Rate

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Optimization of Extraction Conditions for Total Phenolics and Total Flavonoids from <i>Kaempferia parviflora</i>Rhizomes

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Advances in Bioscience and Biotechnology 2018年第5期9卷 205-214页

作者： Zuraida Ab Rahman Shazwan Abd Shukor hartinee abbas Chandradevan A. L. Machap Mohd Suhaimi Bin Alias Razali Mirad Syairah Sofiyanand Ayu Nazreena Othman Biotechnology & amp Nanotechnology Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Horticulture Research Centre MARDI Sintok Bukit Kayu Hitam Malaysia Food Technology Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Agrobiodiversity and Environment Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Nilai Polytechnic Kompleks Pendidikan Bandar Enstek Bandar Enstek Malaysia

Kaempferia parviflora plants derived from in vitro culture were grown in the glasshouse. A comparison of the yield of total phenolics and total flavonoids under varying extraction conditions from rhizomes harvested from plants of different ages was undertaken. The results showed that phenolic and flavonoid contents in the rhizomes were highest 8 months after planting. Another study found that 2 g rhizomes extracted in 50 ml of water at 90°C for 120 minutes gave the best yield of phenolics and flavonoids. Under these conditions, an average of 210 mg GAE/g dry weight of total phenolics and 81 μg QCE/g dry weight of total flavonoids were obtained.

关键词： Phenolics Flavonoids Kaempferia parviflora Extraction

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