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骨保护素抑制肿瘤坏死因子诱导的成骨细胞凋亡(英文)

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中国临床康复 2005年第19期9卷 243-245页

作者：步荣发李亚男 Jennifer I Oakley harry c blair 解放军总医院全军口腔医学研究所口腔颌面外科 Department of Pathology and cell Biology & Physiology University of Pittsburgh School of Medicineand Veteran's Affairs MedicalCenterPittsburghPA15243

背景:骨质疏松涉及破骨细胞分化成熟和成骨类细胞凋亡两方面,肿瘤坏死因子α能诱导多种细胞凋亡且效率差异很大,骨保护素能抑制破骨细胞成熟,但对成骨类细胞凋亡的作用未见报道。目的:探讨肿瘤坏死因子α能否诱导成骨类细胞凋亡及骨保... 详细信息

背景:骨质疏松涉及破骨细胞分化成熟和成骨类细胞凋亡两方面,肿瘤坏死因子α能诱导多种细胞凋亡且效率差异很大,骨保护素能抑制破骨细胞成熟,但对成骨类细胞凋亡的作用未见报道。目的:探讨肿瘤坏死因子α能否诱导成骨类细胞凋亡及骨保护素能否抑制其凋亡。设计:以成骨细胞和MG63细胞作为实验对象、以鼠WEHI164细胞系用于阳性对照的观察对比分析。单位:解放军总医院全军口腔医学研究所、美国匹茨堡医学院退伍军人医疗中心。材料:本实验于2001-01/2003-12在***实验室和口腔研究所实验室完成。选择商品化细胞系,RPMI1640培养液和各种相关蛋白用于实验。方法:用人间充质干细胞在分化培养液中培养21d使之成为成骨细胞,与MG63一起用于凋亡实验。AnnexinV和原位末端标记法检测肿瘤坏死因子α诱导成骨类细胞凋亡。主要观察指标:全部细胞数及凋亡细胞数。结果:和FasL诱导细胞凋亡一样,肿瘤坏死因子α能引起MG63骨肉瘤细胞,间充质干细胞和成骨细胞的凋亡,并表现为明显的浓度依赖和时间依赖。低浓度肿瘤坏死因子α(170～500pmol/L)作用于细胞2～4h就显示了较明显的凋亡,而骨保护素在0.45～1.5nmol/L浓度时,几乎完全抑制了500pmol/L肿瘤坏死因子α诱导的凋亡。成骨细胞分泌骨保护素,而破骨细胞及破骨前体细胞产生肿瘤坏死因子α,它们相互作用降低成骨细胞的凋亡。结论:肿瘤坏死因子α能诱导成骨类细胞凋亡,骨保护素能够抑制肿瘤坏死因子α的凋亡诱导作用,从抑制破骨细胞成熟和成骨细胞凋亡两方面表现骨保护素防治骨质疏松的作用机制。

关键词：骨质疏松肿瘤坏死因子脱噬作用

OPG对TNF诱导的成骨细胞凋亡的抑制作用

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解放军医学杂志 2004年第4期29卷 338-341页

作者：步荣发李亚男 Jennifer I Oakley harry c blair 解放军总医院 Departments of Pathology and cell Biology&Physiology University of Pittsburgh School of Medicineand Veteran’s Affairs Medical CenterPittsburghPA15243

目的　探讨TNF α能否诱导成骨类细胞凋亡及OPG能否抑制其凋亡 ,开辟一条防治骨质疏松的新路。方法　将人间充质干细胞在分化培养液中培养 2 1天使之成为成骨细胞 ,与MG6 3一起用于凋亡实验。鼠WEHI 16 4细胞系用于阳性对照。AnnexinV和... 详细信息

目的　探讨TNF α能否诱导成骨类细胞凋亡及OPG能否抑制其凋亡 ,开辟一条防治骨质疏松的新路。方法　将人间充质干细胞在分化培养液中培养 2 1天使之成为成骨细胞 ,与MG6 3一起用于凋亡实验。鼠WEHI 16 4细胞系用于阳性对照。AnnexinV和TUNEL分析方法检测成骨类细胞凋亡。结果　和FasL诱导细胞凋亡一样 ,TNF α能引起MG6 3骨肉瘤细胞、间充质干细胞和成骨细胞的凋亡 ,并表现为明显的浓度依赖和时间依赖。低浓度TNF α(170～ 5 0 0 pmol/L)作用于细胞 2～ 4h就显示了较明显的凋亡 ,而 0 4 5～1 5nmol/LOPG几乎完全抑制了 5 0 0 pmol/LTNF α诱导的凋亡。结论　OPG能够抑制TNF α诱导的成骨类细胞凋亡。

关键词：肿瘤坏死因子骨质疏松凋亡

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人OPG与肿瘤坏死因子族蛋白的结合研究

军医进修学院学报 2004年第4期25卷 311-313页

作者：步荣发 christopher W Borysenko 李亚男 Sara E Kalla harry c blair 解放军总医院口腔科 Departments of Pathology and cell Biology & Physiology University of PittsburghSchool of Medicine and Veteran's Affairs Medical Center Pittsburgh PA 15243USA

目的 :检测OPG与TNF家族蛋白的结合及探索它们相互作用机制。方法 :免疫沉淀分析加Western印迹 ,酶联免疫吸附测定方法 ,偶联剂交叉链接 ,表面胞质团共振。结果 :OPG与RANKL ,TRAIL和TNFα用不同方法检测均有结合。OPG与TNFα的结合由Bi... 详细信息

目的 :检测OPG与TNF家族蛋白的结合及探索它们相互作用机制。方法 :免疫沉淀分析加Western印迹 ,酶联免疫吸附测定方法 ,偶联剂交叉链接 ,表面胞质团共振。结果 :OPG与RANKL ,TRAIL和TNFα用不同方法检测均有结合。OPG与TNFα的结合由Biacore进一步证实 ,在 pH为 7.4 ,温度在 2 0℃时 ,其离解系数为7.0nmol/L ,同OPG TRAIL亲和力。偶联剂交叉链接显示OPG TNFα结合的比例为 1∶1。结论 :TNFα能与OPG结合 ,物理条件影响其结合。

关键词： OPG 肿瘤坏死因子族蛋白相互作用免疫电泳技术检测

Gut microbiota in cancer initiation,development and therapy

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Science china Life Sciences 2025年

作者： Ruyi Zhang Xiang Zhang harry cheuk Hay Lau Jun Yu Institute of Digestive Disease and Department of Medicine and Therapeutics State Key Laboratory of Digestive DiseaseLi Ka Shing Institute of Health SciencesCUHK Shenzhen Research InstituteThe Chinese University of Hong Kong

cancer has long been associated with genetic and environmental factors,but recent studies reveal the important role of gut microbiota in its initiation and *** 13%of cancers are linked to infectious agents,highlighting the need to identify the specific microorganisms *** microbiota can either promote or inhibit cancer growth by influencing oncogenic signaling pathways and altering immune *** can lead to cancer,while certain probiotics and their metabolites may help reestablish microecological balance and improve anti-tumor immune *** into targeted approaches that enhance therapy with probiotics is ***,the effects of probiotics in humans are complex and not yet fully ***,methods to counteract harmful bacteria are still in *** clinical trials also indicate that modifying gut microbiota may help manage side effects of cancer *** research is crucial to understand better how gut microbiota can be used to improve cancer prevention and treatment outcomes.

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A review on molten sulfate salts induced hot corrosion

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Journal of Materials Science & Technology 2021年第31期90卷 243-254页

作者： Shanshan Hu harry Finkle Xingbo Liu Mechanical&Aerospace Engineering Department Benjamin M.Statler College of Engineering&Mineral ResourcesWest Virginia UniversityMorgantownWV 26506-6106USA c.Eugene Bennett Department of chemistry West Virginia UniversityMorgantownWV 26506USA

Hot corrosion has been extensively observed in various high temperature applications,which might cause large economic *** deepen the insight and understanding of hot corrosion,herein,we provide a detailed discussion on hot corrosion induced by molten sulfate salt,in which Na_(2)SO_(4)is the main chemical *** hot corrosion is triggered and sustained by the negative solubility gradient of protective oxide from the oxide/salt interface to salt/gas *** this work,we reviewed several key factors,including temperature,gas composition,molten salt composition,alloy element and external stress,influencing the hot corrosion of alloy beneath the molten *** application of anti-corrosion coating seems to be the most effective and commercial mothed to mitigate hot ***,the progress of the development of effective coatings,e.g.,Ni-cr coatings,aluminide coatings,silicide coatings and McrAlY(X)coatings,has also been summarized.

关键词： Hot corrosion Molten sulfate salts Protective oxide layer Dissolution Anti-corrosion coatings

Design and guidance of a multi-active debris removal mission

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Astrodynamics 2023年第4期7卷 383-399页

作者： Minduli c.Wijayatunga(B) Roberto Armellin harry Holt Laura Pirovano Aleksander A.Lidtke Te Pūnaha Atea–Space Institute The University of AucklandAuckland 1010New Zealand Astroscale Japan Inc. 1-16-4-16 KinshiSumida-kuTokyoJapan

Space debris have become exceedingly dangerous over the years as the number of objects in orbit continues to *** debris removal(ADR)missions have gained significant interest as effective means of mitigating the risk of collision between objects in *** study focuses on developing a multi-ADR mission that utilizes controlled reentry and *** mission comprises two spacecraft:a Servicer that brings debris to a low altitude and a Shepherd that rendezvous with the debris to later perform a controlled reentry.A preliminary mission design tool(PMDT)was developed to obtain time and fuel optimal trajectories for the proposed mission while considering the effect of J2,drag,eclipses,and duty *** PMDT can perform such trajectory optimizations for multi-debris missions with computational time under a *** guidance schemes are also studied,taking the PMDT solution as a reference to validate the design methodology and provide guidance solutions to this complex mission profile.

关键词： active debris removal(ADR) trajectory design optimization autonomous guidance low-thrust electric propulsion △u-law guidance Q-law guidance

Bandwidth optimization of a Planar Inverted-F Antenna using binary and real coded genetic algorithms

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重庆邮电大学学报（自然科学版） 2009年第2期21卷 276-283页

作者： AMEERUDDEN Mohammad Riyad RUGHOOPUTH harry c S Department of Electronics and communication Engineering University of Mauritius Reduit Mauritius Department of Electrical and Electronic Engineering University of Mauritius Reduit Mauritius

With the exponential development of mobile communications and the miniaturization of radio frequency transceivers, the need for small and low profile antennas at mobile frequencies is constantly growing. Therefore, new antennas should be developed to provide larger bandwidth and at the same time small dimensions. Although the gain in bandwidth performances of an antenna are directly related to its dimensions in relation to the wavelength, the aim is to keep the overall size of the antenna constant and from there, find the geometry and structure that give the best performance. The design and bandwidth optimization of a Planar Inverted-F Antenna (PIFA) were introduced in order to achieve a larger bandwidth in the 2 GHz band, using two optimization techniques based upon genetic algorithms (GA), namely the Binary coded GA (BcGA) and Real-coded GA (RcGA). During the optimization process, the different PIFA models were evaluated using the finite-difference time domain (FDTD) method-a technique belonging to the general class of differential time domain numerical modeling methods.

关键词：实数编码遗传算法平面倒F天线带宽优化二进制编码有限差分时域数值模拟方法天线性能优化技术

Lexical characteristics of dialogue acts in the Switchboard corpus of telephone conversations

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外语教学与研究 2012年第3期44卷 346-358页

作者： Alex c. Fang Jing cao Xiaoyue Liu harry Bunt city University of Hong Kong Zhongnan University of Economics and Law Tilburg University

This paper describes a corpus-based investigation of dialogue *** particular,it attempts to answer questions about the empirical distribution of dialogue acts in a publicly accessible corpus of transcribed conversations and degrees of lexical similarity of dialogue *** research will not only lend itself to a deeper understanding of the lexical realisation of dialogue acts as communicative functions but also contribute towards dialogue act *** Switchboard Dialogue Act corpus is adopted and the SWBD-DAMSL tags used for the *** statistical measures were applied,which include lexical dispersion and lexical similarity using chi by degrees of freedom(cBDF).We show that the distribution of DAs is uneven,that a majority of the word types occur in one DA and that only a small fraction of word types are commonly used by all the *** hierarchical clustering of DAs based on cBDF suggests that there is an intrinsic structure of DAs pertaining to fact finding and verification,information provision and inter-speaker agreement and affinity *** paper concludes with discussions and suggestions for future work.

关键词： Dialogue Acts(DA) conversation Switchboard corpus

TWO NOVEL GRADIENT METHODS WITH OPTIMAL STEP SIZES

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Journal of computational Mathematics 2021年第3期39卷 375-391页

作者： harry Oviedo Oscar Dalmau Rafael Herrera centro de Investigacion en Matematicas CIMAT A.C.GuanajuatoGto.Mexico

In this work we introduce two new Barzilai and Borwein-like steps sizes for the classical gradient method for strictly convex quadratic optimization *** proposed step sizes employ second-order information in order to obtain faster gradient-type *** step sizes are derived from two unconstrained optimization models that involve approximate information of the Hessian of the objective function.A convergence analysis of the proposed algorithm is *** numerical experiments are performed in order to compare the efficiency and effectiveness of the proposed methods with similar methods in the ***,it is observed that our proposals accelerate the gradient method at nearly no extra computational cost,which makes our proposal a good alternative to solve large-scale problems.

关键词： Gradient methods convex quadratic optimization Hessian spectral properties Steplength selection