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Preterminal protein,the achilles heel of adenoviridae:Implications for adenoviral infections

World Journal of Pharmacology 2024年第2期13卷 1-9页

作者： harold a walsh Faculty of Pharmacy Division of PharmacologyRhodes UniversityGrahamstown 6139Eastern CapeSouth Africa

Adenoviruses pose a serious health risk particularly in the absence of any clinically approved *** adenoviral infections are quite frequent and recent outbreaks manifest more virulent variant strains,the need to develop an effective treatment remains a *** adenoviral protein,preterminal protein(pTP),is one of the key common products of the viral lifecycle as it is necessary to initiate viral replication and hence the infection *** makes pTP a potential chemotherapeutic target in the search for and development of an effective treatment for adenoviral induced *** we report,for the first time,that glycosylation of pTP in situ prevents binding to ssDNA in *** To explore whether specific structural tailoring of the adenoviral protein pTP,imparts the potential to scupper the viral replication *** All chemicals used were of reagent *** of pTP was achieved using the‘BAC to BAC’expression *** presence and relative concentration of the protein was determined throughout the incubation period by the Bradford *** pTP was identified by MALDI-TOFF and sodium dodecyl sulphate polyacrylamide gel *** the removal of the aminosugar,a deglycosylase enzyme kit from PROZYME was *** of cloned pTP(6xHis)was done with a ssDNA cellulose column followed by a Ni-NTA ***-tags were excised with the Tobacco etch virus *** fractionation was performed with a fraction collector coupled to a UV detector(280 nm)from *** The pTP overexpressed in insect cells(Spodoptera frugiperda)(>96 hours),is unable to bind to ssDNA in *** of this unbound protein with a deglycosidase enzyme that is specific for the removal of truncated unsubstituted O-linked Galβ(1-3)GalNAc-α1 disaccharides bound to Thr or Ser in a glycoprotein,restores binding to *** is presented as a linegraph for both the glycosylated and the deglycosylated *** point repr

关键词： Thomsen-Friedenreich antigen Adenoviral Preterminal protein Spodoptera frugiperda cells Protein priming mechanism Glycosylation

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Optimisation of the Design of Pulverised Coal Injection Lances:From Concept to Full Scale Implementation at BlueScope Steel’s BF6

Optimisation of the Design of Pulverised Coal Injection Lanc...

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第五届国际炼铁科技大会

作者： Daniel Maldonado Paul Zulli Peter Austin Mark walsh John Mathieson harold Rogers BlueScope Steel Research Port Kembla BlueScope Steel Ironmaking Department Port Kembla BHP Billiton-Newcastle Technology Centre

Pulverised coal injection started at BlueScope Steel’s Port Kembla blast furnaces in 2002. With significant economic advantages to reduce consumption of coking coal via increasing the quantity of pulverised coal injected to the blast furnaces, a three-dimensional numerical model of the blowpipe/tuyere/raceway has been developed. The model has been applied in various plant-specific investigations of blast parameters, and more recently in the development of alternate coal injection lance tip geometries. The designs with the highest potential for increasing coal burnout included swirl and high dispersive tip designs. The reduction in char load entering the furnace for the best performing alternate designs was due to an increase in coal dispersion compared with the conventional design. A full-scale trial of the swirl lance design was carried out at Port Kembla’s BF6 in mid-2008. The trial delivered promising results, with PCI rates maintained. This was despite unrelated changes to the coke ash content and the burden mix fed to the furnace, which would normally reduce furnace permeability.

关键词： coal combustion modelling injection lance tip design char burnout

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The Training and Performance of Emergency Physicians as Anesthetists for International Medical Surgical Response Teams: The Emergency Physician’s General Anesthesia Syllabus (EP GAS)

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Open Journal of Anesthesiology 2014年第2期4卷 53-61页

作者： Richard Skupski Mark walsh Manar Jbara Donald Zimmer Bhavesh M. Patel Michael T. McCurdy James Lantry Braxton Fritz Patrick Davis Harsha Musunuru Anne Newbold Art Toth Richard Frechette Tiffany Alexander Madhura Sundararajan John Lovejoy Dan Hottinger Joe Capannari Rachel Kurcz Gerard Bernard harold Previl Department of Anesthesia and Trauma Services Memorial Hos-pital of South Bend South Bend USA Department of Anesthesia and Trauma Services Memorial Hos-pital of South Bend South Bend USA Sacred Heart Hospital Milot Haiti Department of Anesthesia and Trauma Services Memorial Hos-pital of South Bend South Bend USA St. Damien and St. Luc’s Hospital Port au Prince Haiti Department of Anesthesia and Trauma Services Memorial Hos-pital of South Bend South Bend USA St. Damien and St. Luc’s Hospital Port au Prince Haiti Sacred Heart Hospital Milot Haiti Department of Anesthesia and Trauma Services Memorial Hos-pital of South Bend South Bend USA St. Damien and St. Luc’s Hospital Port au Prince Haiti Mayo Clinic—Emergency Medicine Residency Program Rochester USA Sacred Heart Hospital Milot Haiti St. Damien and St. Luc’s Hospital Port au Prince Haiti St. Damien and St. Luc’s Hospital Port au Prince Haiti University of Maryland School of Medicine Baltimore USA St. Damien and St. Luc’s Hospital Port au Prince Haiti University of Maryland School of Medicine Baltimore USA. St. Damien and St. Luc’s Hospital Port au Prince Haiti Mayo Clinic—Critical Care Medicine Scottsdale USA

Background: The availability of an anesthesiologist is often a limiting factor in the number of operations that can be performed by International Medical Surgical Response Teams (IMSuRT). Because emergency physicians (EPs) possess skills in airway control, management of moderate and deep sedation, and ventilator management, we propose that with proper training in general anesthesia, EPs can serve as anesthetists for IMSuRT with anesthesiologist supervision. Methods: During a 10-week period, a board-certified EP administered general anesthesia to 60 patients prior to a surgical medical mission trip. The breakdown of surgical cases was: 11 orthopedic, 2 genitourinary, 20 ear, nose, and throat, 8 obstetrics and gynecological, 13 general surgery, and 6 vascular. A simplified protocol for induction, maintenance, and emergence was adhered to for all cases. Results: Fourteen orthopedic cases using general anesthesia were performed in a one-week period in Haiti. These cases involved open reduction and internal fixation (ORIF), hemiarthoplasty, hardware removal, tendon transfer and external fixation of fractured bone. Conclusion: We demonstrate the feasibility of a model curriculum to train EPs in the basics of anesthesia. The EP can safely and effectively deliver general anesthesia for major cases on surgical medical mission trips under the auspices of an anesthesiologist in an austere environment.

关键词： Emergency Physicians General Anesthesia Syllabus International Medical Surgical Response Teams Anesthetists Induction Maintenance Emergence Anesthesia

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肾功能和疾病规范命名:改善全球肾脏病预后(KDIGO)会议共识

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临床儿科杂志 2022年第8期40卷 627-633页

作者：王忍高春林夏正坤张沛(译) 东部战区总医院儿科江苏南京210002

为规范描述肾功能和疾病的专业术语,2019年6月改善全球肾脏病预后组织(KDIGO)召开共识会议,对肾脏功能和疾病、肾衰竭、急性肾脏病和急性肾损伤、慢性肾脏病及肾脏评估共5个部分的内容制定了相应的术语表,并就以下建议达成共识。①对于... 详细信息

为规范描述肾功能和疾病的专业术语,2019年6月改善全球肾脏病预后组织(KDIGO)召开共识会议,对肾脏功能和疾病、肾衰竭、急性肾脏病和急性肾损伤、慢性肾脏病及肾脏评估共5个部分的内容制定了相应的术语表,并就以下建议达成共识。①对于肾脏功能和疾病的一般描述,使用术语“kidney”,而不使用“renal”或前缀“nephro-”;②使用“肾衰竭(kidney failure)”并适当描述是否存在相应症状、体征和治疗,而不是“终末期肾脏疾病(end-stage kidney disease)”;③使用KDIGO对急性肾脏病(AKD)和急性肾损伤(AKI)进行定义和分类;④使用KDIGO对慢性肾脏病(CKD)进行定义和分类;⑤使用特定的肾脏检测指标,例如蛋白尿或肾小球滤过率(GFR)降低,而不是肾功能“异常(abnormal)”或“降低(reduced)”来描述肾脏结构和功能的改变。文章简要总结会议的主要讨论。

关键词：肾脏功能肾脏疾病术语

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