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An Imperative Role of Fetal Autopsy in Previable Fetuses-Amniotic Deformity Adhesions,Mutilations Complex

Maternal-Fetal Medicine 2024年第3期6卷 134-140页

作者： harini devi jka Nidhya Ganesan Umamaheswari Gurusamy Department of Pathology PSG Institute of Medical Sciences and ResearchPeelameduCoimbatore 641004India

Objective:To identify the occurrence of the amniotic deformity adhesions,mutilation(ADAM)complex and imperative role of fetal autopsy in diagnosing this ***:A retrospective descriptive study spanning nine years,from January 2014 to January 2022,was conducted at the Department of Pathology within a tertiary care hospital in South *** study focused on analyzing the clinical presentation,prenatal ultrasonogram,and morphological features of fetuses with the ADAM complex,limb body wall complex,or amniotic ***:Among the 438 fetuses assessed during the study period,five fetuses showed features of the ADAM complex(0.01%).The most frequent gestational age observed was 12–18 weeks andmost fetuseswere *** common anomaly encountered was limb defects,followed by abdominal and cranial ***:The diagnosis of the ADAM complex relies primarily on fetal autopsy to differentiate it from similar conditions like anencephaly or body-stalk *** pathologist plays a crucial role in understanding the complexities of the ADAM *** antenatal imaging and therapies offer potential for prevention through improved counseling.

关键词： Amniotic band syndrome ADAM complex Previable fetus Fetal autopsy

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Screening and molecular characterization of Serratia marcescens VITSD2： A strain producing optimum serratiopeptidase

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Frontiers in Biology 2013年第6期8卷 632-639页

作者： C. Subathra devi Renuka ELIZABETH JOSEPH harini SARAVANAN S. Jemimah NAINE V. Mohana SRINIVANSAN School of Biosciences and Technology VIT University VeIlore Tamil Nadu India

The current work was attempted to isolate and characterize the serratiopeptidase producing Serratia sp. Among the 10 bacterial isolates 7 strains were identified as Serratia sp. Out of 7 strains one showed potent proteolytic activity and selected for further studies. Based on the morphological, biochemical and molecular characterization, the potent isolate （RH03） was identified as Serratia marcescens （GenBank accession number： KC961637） and the strain was designated as Serratia marcescens VITSD2. The production of serratiopeptidase was carried out in trypticase soya broth and the enzyme was partially purified using ammonium sulfate precipitation and dialysis. The specific activity was determined by casein hydrolysis assay and was found to be 12.00, 21.33, and 25.40 units/rag for crude, precipitated and dialysed samples. The molecular weight of the protease was determined by SDS-PAGE and it was found to be 50 kDa. The antibacterial activity of the produced serratiopeptidase showed moderate activity against Pseudomonas aeruginosa MTCC No. 4676 （12 mm） and Escherichia coli MTCC No. 1588 （15 mm）.

关键词： Serratiopeptidase Serratia marcescens VITSD2 antibacterial activity

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