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Digital light processing 3D printing for microfluidic chips with enhanced resolution via dosing-and zoning-controlled vat photopolymerization

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Microsystems & Nanoengineering 2023年第4期9卷 315-327页

作者： Zhiming Luo haoyue zhang Runze Chen Hanting Li Fang Cheng Lijun zhang Jia Liu Tiantian Kong Yang zhang Huanan Wang School of Biomedical Engineering Shenzhen University Health Science CenterShenzhen 518000P.R.China State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Oriented Chemical EngineeringSchool of BioengineeringDalian University of TechnologyDalian 116024P.R.China Third People’s Hospital of Dalian Dalian Eye HospitalDalian 116024 P.R.China Central Laboratory The Second Affiliated Hospital of TheChinese University of Hong KongShenzhen 518172P.R.China

Conventional manufacturing techniques to fabricate microfluidic chips,such as soft lithography and hot embossing process,have limitations that include difficulty in preparing multiple-layered structures,cost-and labor-consuming fabrication process,and low *** light processing(DLP)technology has recently emerged as a costefficient microfabrication approach for the 3D printing of microfluidic chips;however,the fabrication resolution for microchannels is still limited to sub-100 microns at ***,we developed an innovative DLP printing strategy for high resolution and scalable microchannel fabrication by dosing-and zoning-controlled vat photopolymerization(DZC-VPP).Specifically,we proposed a modified mathematical model to precisely predict the accumulated UV irradiance for resin photopolymerization,thereby providing guidance for the fabrication of microchannels with enhanced *** fine-tuning the printing parameters,including optical irradiance,exposure time,projection region,and step distance,we can precisely tailor the penetration irradiance stemming from the photopolymerization of the neighboring resin layers,thereby preventing channel blockage due to UV overexposure or compromised bonding stability owing to insufficient resin ***,this strategy can allow the preparation of microchannels with cross-sectional dimensions of 20μm×20μm using a commercial printer with a pixel size of 10μm×10μm;this is significantly higher resolution than previous *** addition,this method can enable the scalable and biocompatible fabrication of microfluidic drop-maker units that can be used for cell *** general,the current DZC-VPP method can enable major advances in precise and scalable microchannel fabrication and represents a significant step forward for widespread applications of microfluidics-based techniques in biomedical fields.

关键词： printing resolution resin

Biocompatible, small-sized and well-dispersed gold nanoparticles regulated by silk fibroin fiber from Bombyx mori cocoons

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Frontiers of Materials Science 2019年第2期13卷 126-132页

作者： Chengzhi YANG Shikun CHEN Huilan SU haoyue zhang Jianfei TANG Cuiping GUO Fang SONG Wang zhang Jiajun GU Qinglei LIU State Key Laboratory of Metal Matrix Composites School of Materials Science and Engineering Shanghai Jiaotong University Shanghai 200240 China

Biocompatible, small-sized but well-dispersed gold nanoparticles (Au NPs) remain a major challenge for their synthesis. Here a convenient solution impregnation technique is developed to prepare such Au NPs under the regulation of degummed silk fibroin fibers (SFFs) extracted from Bombyx mori cocoons. SFFs play multiple roles in the formation of Au NPs such as reactive substrate to capture AuCl4^- ions by the chelation of -C = 0, reducing agent for Au(0) by the reduction of -OH , and modifiers to render biocompatible Au NPs by some functional groups and biomolecules. The as-prepared Au NPs with a size of 7-10 nm are embedded in the solid SFF substrate, and can disperse well in the liquid system by the disintegration of SFFs into silk fibroin (SF) in a certain CaCl2 solution. The biocompatible Au NPs exhibit uniform small size and distribute stably in both solid and solution states, which have distinctive properties and functional advantages, and bring great convenience to their storage and transportation.

关键词： Au NPs biocompatibility small size dispersivity silk fibroin fiber

Dynamics of Haptophytes (Eukaryota, Hacrobia) Revealed via Short-Term Sampling in the South China Sea

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JOURNAL OF OCEAN UNIVERSITY OF CHINA 2024年第6期23卷 1677-1688页

作者： zhang, haoyue Li, Ran Gu, Bowei Kong, Hejun Xu, Jie zhang, Rui Li, Xiaolin Jiao, Nianzhi Xu, Dapeng Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China Xiamen Univ Fujian Key Lab Marine Carbon Sequestrat Inst Marine Microbes & Ecospheres Xiamen 361102 Peoples R China Univ Macau Fac Sci & Technol Ctr Reg Oceans Dept Ocean Sci & Technol Macau 999078 Peoples R China Shenzhen Univ Inst Adv Study Shenzhen 518060 Peoples R China

Haptophytes (Eukaryota, Hacrobia) play a crucial role in the energy budget and element cycling of diverse aquatic ecosystems due to their ability to engage in both phototrophic and mixotrophic nutritional modes. Nevertheless, there is a significant lack of knowledge regarding the short-term variations, such as diel dynamics, of their ecological features. During a short time frame in the summer of 2018, samples were collected from three distinct water layers in the South China Sea, including surface water, the deep chlorophyll maximum (DCM) layer, and 200 m depth. Fluorescence in situ hybridization coupled with tyramide signal amplification was used to quantify haptophyte cell abundance. Most haptophyte communities in all three water layers were composed of cells 2-5 mu m in size, while the proportion of cells m increased with water depth. High-throughput sequencing of the V4 hypervariable regions of the SSU rRNA revealed that Chrysochromulina and Phaeocystis predominated the community, and the former was more abundant in the surface water and 200 m depth and the latter was more abundant in the DCM layer. Higher abundance of small cells (m and 2-5 mu m) during the night was found compared to the day time, whereas large cells (5-10 mu m and 10-20 mu m) were more prevalent during the day time. The results of correlation analyses showed that haptophyte abundance was possibly impacted by both environmental biotic (heterotrophic nanoflagellates, heterotrophic bacteria, and viruses) and abiotic (temperature, salinity, and nutrients) factors.

关键词： eukaryotic phytoplankton FISH-TSA high-throughput sequencing microbial eukaryotes size fraction

Water Resources Management:A Review of the Process-based Hydrologic Models

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Water Resources Management:A Review of the Process-based Hyd...

Semi-tensor Product Approach to Approximation of the Constrained Joint Spectral Radius

作者： haoyue zhang Jie NIU School of Water Resources and Environment China University of Geosciences(Beijing) Institute of Groundwater and Earth Sciences Jinan University

Process-based hydrologic models are a powerful tool to study a variety of hydrological responses,simulate contaminant transport and comprehensively manage water resources exposed to climate change and human activities across a range of spatio-temporal *** to strong non-linearity and transient change of climate and land surface,it’s necessary to understand the mechanism of natural processes in *** hydrologic models should be based on physics and coupled with other *** paper aims to emphasize the significance of this kind of *** compares strengths and weaknesses,conditions and scope of application of different Process-based hydrologic models,and discusses current *** research will emphasize interdisciplinarity and combine hydrology with climatology,ecology,and so ***,the innovation of theories and fusion among multisource data should be promoted in the future.

关键词： physical process distribution hydrologic model

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Semi-tensor Product Approach to Approximation of the Constra...

Late Quaternary tectonic uplift and Yellow River evolution create high arsenic aquifers in the Hetao Basin,China

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作者： haoyue zhang Yang Song Li Deng School of Mechatronic Engineering and Automation Shanghai University

In this paper, a semi-tensor product（STP） approach is proposed to discuss the stability problem of a constrained switching system（CSS）. The switching sequence is subject to the constraint of a nondeterministic finite automaton（NFA）. By the mathematical tool, the constrained joint spectral radius（CJSR） of the CSS can be approximated. We propose the equivalence of a matrix-based expression of the CSS and its lifted l-dimensional switching system by using the STP approach. After that, the approximate relation between the constrained joint spectral radius（CJSR）/the constrained generalized spectral radius（CGSR）and the joint spectral radius（JSR）/generalized spectral radius（GSR） is developed for the switching system. At the same time,there is a special case of NFA, where the equivalence relation between them also is proved.

关键词： Constrained Joint Spectral Radius Constrained Switching System Semi-tensor Product

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Geoscience Frontiers 2024年第6期15卷 167-175页

作者： haoyue zhang Xujiao zhang Peisheng Ye Chenglu Li Junlei Li Xiaoning Yuan Xiangge zhang Huaming Guo Pat J.-F.Yeh Yanchi Research Station School of Soil and Water ConservationBeijing Forestry UniversityBeijing 100083China School of Earth Sciences and Resources China University of GeosciencesBeijing 100083China Institute of Geomechanics Chinese Academy of Geological SciencesBeijing 100081China School of Water Resources and Environment China University of GeosciencesBeijing 100083China Department of Civil Engineering Monash UniversityMalaysia CampusMalaysia

High arsenic(As)groundwater is a global problem primarily originating from As-enriched *** provenance(source)and release mechanisms(sinks)of high As sediment have been identified,but the source-sink transfer is poorly understood,especially the influence of geological and surface *** this study,we explore the roles of tectonic movement and Yellow River evolution in provenance formation processes and evaluate the combined effects of provenance and sediment age on the As content of aquifer sediments in the northern Hetao Basin of Inner *** on optically stimulated luminescence(OSL)and 14C dating and detrital zircon U-Pb,As content,and lithological analyses of a 400 m core,we reconstructed As changes over the last 160 *** results show clay deposited in a paleo-lake during the Gonghe movement period in the late Pleistocene(∼100 ka B.P.)is enriched in As(31.8μg/g)due to significant provenance contributions of the As-bearing Langshan Group under tectonic uplift and mountain *** contrast,clay deposited in the middle Pleistocene(∼160 ka B.P.)has lower As content(7.3μg/g)due to the Yellow River as the primary ***,the provenance of basin As forced by tectonic uplift and Yellow River evolution determines the background As of aquifer *** deposition,sediment As content decays over time,with higher decay rates in coarse-grained sands than ***,both provenance formation and sediment age,representing initial and dynamic states of solid phase As,jointly determine the As content of aquifer *** solid phase As provided by younger sediments from the proximal orogenic provenance and reducing conditions due to frequent river-lake transitions,jointly lead to higher As concentrations in shallow *** study highlights the potential for using a combined analysis of the tectonic movement-surface processes-environment system to improve understanding of geogenic high As groundwater over glob

关键词： High arsenic groundwater Provenance Tectonic uplift Yellow River evolution Surface processes Hetao Basin

国债收益率与高频宏观因子--基于非规则混频利率期限结构模型

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统计研究 2023年第10期40卷 109-123页

作者：尚玉皇张皓越西南财经大学中国金融研究院

及时反映金融市场与宏观经济的动态作用机制是大数据时代制定前瞻性货币政策和投资决策分析的关键。重大突发事件增加了动态经济机制的不确定性,有必要挖掘高频信息加以识别。为此,本文提出一种非规则混频宏观利率期限结构(IR-MF-NS)模... 详细信息

及时反映金融市场与宏观经济的动态作用机制是大数据时代制定前瞻性货币政策和投资决策分析的关键。重大突发事件增加了动态经济机制的不确定性,有必要挖掘高频信息加以识别。为此,本文提出一种非规则混频宏观利率期限结构(IR-MF-NS)模型,并基于我国国债收益率和宏观经济信息进行检验。研究发现:与传统模型相比,新模型国债收益率的拟合效果有所改善;国债收益率期限结构与宏观经济存在相互作用机制,实体经济活动与斜率因子呈负相关关系,这与我国逆周期监管目标相一致;高频曲度因子对通货膨胀产生显著正向持续性冲击,验证了曲度因子在高频数据中发挥重要作用的结论;经济机制受信息频率影响,低频数据的“平滑效应”使得低频经济机制表现出平稳效果;高频数据因为信息波动产生“反转效应”使得低频机制和高频机制出现背离;受到重大突发事件的冲击后,国债收益率与宏观基本面的联动性出现系统性减弱,期限结构因子的预测方差作用降低,而宏观基本面对国债收益率预测方差的贡献增大。

关键词：高频宏观因子利率期限结构非规则混频

基于生态系统的海岸带综合管理研究——以广东省湛江市为例

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海洋湖沼通报 2023年第6期45卷 171-179页

作者：褚晓琳张皓玥上海海洋大学海洋文化与法律学院上海201306

基于生态系统的海岸带综合管理是统筹考虑海洋生态系统各种因素相互作用,通过立法、规划、执法等手段实现海洋环境资源的可持续管理。湛江市作为广东省海洋经济发展重要城市,以及我国“一带一路”建设的重要开发城市,在实施基于生态系... 详细信息

基于生态系统的海岸带综合管理是统筹考虑海洋生态系统各种因素相互作用,通过立法、规划、执法等手段实现海洋环境资源的可持续管理。湛江市作为广东省海洋经济发展重要城市,以及我国“一带一路”建设的重要开发城市,在实施基于生态系统的海岸带综合管理方面较具代表性。随着湛江市海洋经济的迅速发展,其海岸带环境资源也遭受日益严重的破坏。然而,目前湛江市海岸带管理还未真正落实生态系统理念,在海岸带立法、协调机制、执法力量、公众参与四个方面仍存在一些问题。对此,今后湛江市应通过采取以下措施:制定海岸带保护条例、建立海岸带统筹协调机制、建立海岸带联合执法机制、鼓励公众参与海岸带保护等,进一步强化基于生态系统的海岸带综合管理。

关键词：生态系统海岸带综合管理广东省湛江市

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基于全局稀疏地图的AGV视觉定位技术

北京航空航天大学学报 2019年第1期45卷 218-226页

作者：张浩悦程晓琦刘畅孙军华北京航空航天大学仪器科学与光电工程学院北京100083

为了实现自动导引车(AGV)在复杂工业环境下的高精度定位,克服环境变化给定位带来的影响,提出了基于全局稀疏地图的视觉定位方法。首先,设计了大容量二维编码点,作为人工路标铺设在工业环境的地面;然后,基于一种四边形识别算法,在复杂工... 详细信息

为了实现自动导引车(AGV)在复杂工业环境下的高精度定位,克服环境变化给定位带来的影响,提出了基于全局稀疏地图的视觉定位方法。首先,设计了大容量二维编码点,作为人工路标铺设在工业环境的地面;然后,基于一种四边形识别算法,在复杂工业环境中准确分割和识别二维编码点;最后,利用二维编码点提供的编码信息,鲁棒匹配图像中的特征点,并以此为基础,使用一种分参数块优化的三维重建策略,实现了工业环境的大规模地图构建,为AGV视觉定位提供了一种稀疏电子地图。AGV视觉的定位通过匹配车载视觉传感器图像中的特征点和稀疏电子地图实现。停车重复定位精度小于0. 5 mm,角度偏差小于0. 5°,轨迹平均位移误差小于0. 1%。实际应用结果表明,该方法能在复杂工业环境中实现AGV视觉的定位,定位的速度和精度方面都满足工业应用的要求,为AGV的视觉定位提供了新的思路。

关键词：自动导引车(AGV) 视觉定位三维重建稀疏地图编码点