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Alternative splicing of a carboxyl/choline esterase gene enhances the fenpropathrin tolerance of Tetranychus cinnabarinus

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Insect Science 2023年第5期30卷 1255-1266页

作者： Peng Wei Xinying Zeng haonan han Yiqing Yang Youjun Zhang Lin He Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina Academy of Agricultural Sciences Southwest UniversityChongqingChina National Citrus Engineering Research Center Southwest UniversityChongqingChina Department of Plant Protection Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingChina

Detoxification plays a crucial role in agricultural pests to withstand pesticides,and cytochrome P450s,carboxyl/choline esterases(CCEs),and glutathione-S-transferases are the main proteins responsible for their detoxification *** activity of CCEs can be upregulated,downregulated,or modified by ***,few studies have examined the role of alternative splicing in altering the properties of *** identified 2 variants of TcCCE23 in Tetranychus cinnabarinus:a long version(CCE23-V1)and a short version that is 18 nucleotides shorter than CCE23-V1(CCE23-V2).Whether splicing affects the activity of TcCCE23 remains *** of CCE23-V2 in fenpropathrin-resistant *** revealed that splicing affected the detoxification of fenpropathrin by *** mortality of mites was significantly higher when the expression of CCE23-V2 was knocked down(43.2%±3.3%)via injection of CCE23-dsRNA(double-stranded RNA)compared with the control group injected with green fluorescent protein-dsRNA under fenpropathrin exposure;however,the downregulation of CCE23-V1(61.3%±6.3%)by CCE23-small interfering RNA had no such effect,indicating CCE23-V2 plays a greater role in xenobiotic metabolism than *** tolerance of flies overexpressing CCE23-V2 to fenpropathrin(50%lethal dose[LD_(50)]=19.47μg/g)was significantly higher than that of Gal4/UAS-CCE23-V1 transgenic flies(LD_(50)=13.11μg/g).Molecular docking analysis showed that splicing opened a“gate”that enlarges the substrate binding cavity of CCE23-V2,might enhance the ability of CCE23-V2 to harbor fenpropathrin *** findings suggest that splicing might enhance the detoxifying capability of ***,our data improve the understanding of the diversity and complexity of the mechanisms underlying the regulation of CCEs.

关键词： alternative splicing carboxyl/choline esterase(CCE) detoxification function pyrethroid pests

Identification of Novel Bartonella washoensis Sequence Type 22 in Marmota himalayana-Jiuquan City,Gansu Province,China,2021–2022

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China CDC weekly 2023年第20期5卷 442-445,I0001页

作者： Ran Duan Xiaojin Zheng Qun Duan Asaiti Bukai Peng Zhang Shuai Qin Xinmin Lu Dongyue Lyu haonan han Dan Zhang Zhaokai He Junrong Liang Deming Tang Jinxiao Xi Huaiqi Jing Xin Wang State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Akesai Kazak Autonomous County Center for Disease Control and Prevention JiuquanChina Gansu Provincial Center for Disease Control and Prevention LanzhouChina

Summary What is already known about this topic?The prevalence of rodent-adapted Bartonella species has been increasing ***,the specific Bartonella species carried by Marmota himalayana(***),a large rodent species,and the potential risk it poses to human populations remain *** is added by this report?Bartonella washoensis(***),associated with human endocarditis,was initially identified in ***,exhibiting a detection rate of approximately one-third and demonstrating a predilection for the heart and *** discovery of the novel Sequence Type 22 has expanded both the isolation source and genetic lineage of *** are the implications for public health practice?Individuals residing within the *** plague focus are at an elevated risk for *** ***,there is a pressing need for public health warnings and efficient clinical case identification in this population.

关键词： Barton expanded initially

Acousto-optic tunable ultrafast laser with vector-mode-coupling-induced polarization conversion

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Photonics Research 2019年第7期7卷 113-120页

作者： Yujia Li Ligang Huang haonan han Lei Gao Yulong Cao Yuan Gong Wending Zhang Feng Gao Iroegbu Paul Ikechukwu Tao Zhu Key Laboratory of Optoelectronic Technology & Systems Ministry of EducationChongqing UniversityChongqing 400044China Key Laboratory of Optical Fiber Sensing and Communications Ministry of EducationUniversity of Electronic Science and Technology of ChinaChengdu 611731China MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions and Shaanxi Key Laboratory of Optical Information Technology School of ScienceNorthwestern Polytechnical UniversityXi'an 710072China MOE Key Laboratory of Weak-Light Nonlinear Photonics TEDA Applied Physics Institute and School of PhysicsNankai UniversityTianjin 300457China

Acousto-optic interactions, employed in the ultrafast laser regulation,possess remarkable advantages for fast tuning performance in a wide spectral range. Here, we propose an ultrafast fiber laser whose wideband tunability is provided by an acousto-optic structure fabricated with an etched single-mode fiber. Because of the laser polarization conversion induced by the coupling between the core and cladding vector modes in the etched fiber,a band-pass characteristic of the acousto-optic interaction is achieved to effectively regulate the inner-cavity gain range. Cooperating with a saturable absorber based on single-wall carbon nanotubes (SWCNTs) with polarization robustness,a soliton operating state is achieved in the tunable erbium-doped fiber *** controlling the acoustical wave frequency from 1.039 to 1.069 MHz, this soliton laser can be conveniently tuned in a wide spectral range from 1571.52 to ***, the laser pulses have near-transform-limited durations stably maintaining less than 2 ps at different wavelength channels,owing to the broadband nonlinear absorption of SWCNTs.

关键词： polarization Acousto-optic interactions nonlinear

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图像去雾方法质量评价

光学精密工程 2022年第6期30卷 721-733页

作者：韩昊男钱锋吕建威张葆中国科学院长春光学精密机械与物理研究所吉林长春130033 中国科学院大学北京100049

针对近年来去雾算法质量的评价方法普遍依赖主观评价结果,但缺乏定量描述;现有的客观质量评价方法与主观评价方法之间的一致性不稳定,使两者有时会出现分歧的问题。为提升针对去雾方法的客观质量评价性能,本文提出了一种基于人工合成图... 详细信息

针对近年来去雾算法质量的评价方法普遍依赖主观评价结果,但缺乏定量描述;现有的客观质量评价方法与主观评价方法之间的一致性不稳定,使两者有时会出现分歧的问题。为提升针对去雾方法的客观质量评价性能,本文提出了一种基于人工合成图像的全参考去雾方法质量评价。首先,建立合成图像数据集,数据集包括参考无雾图像,合成有雾图像,对这些合成有雾图像使用8种主流去雾算法得到的去雾图像。然后,将去雾后图像可能引入的一些质量问题进行分类。最后,通过结合清晰度相关特征和现有的客观质量评价,针对性地提出了一种由图像可视性、结构相似性和颜色恢复度相互融合的去雾方法质量评价。在合成图像数据集中,将本文方法与现有典型的图像质量评价方法进行对比实验,实验结果表明:对于合成图像数据集,本文提出的方法在SRCC、PLCC和RMSE指标上表现最优。本文方法与主观评价的一致性更高,更有利于支持去雾算法的研究。

关键词：图像去雾客观质量评价合成有雾图像图像数据集

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结合天空分割和雾气浓度估计的图像去雾

光学精密工程 2022年第4期30卷 464-477页

作者：吕建威钱锋韩昊男张葆中国科学院长春光学精密机械与物理研究所吉林长春130033 中国科学院大学北京100049

针对图像去雾算法在天空区域出现恢复不真实以及雾气浓度估计精度不足等问题,本文提出天空区域分割和雾气浓度估计的去雾算法。首先,为了提高透射率的估计精度以及提升去雾效果,利用梯度阈值和亮度阈值分割天空区域。其次,采用改进的暗... 详细信息

针对图像去雾算法在天空区域出现恢复不真实以及雾气浓度估计精度不足等问题,本文提出天空区域分割和雾气浓度估计的去雾算法。首先,为了提高透射率的估计精度以及提升去雾效果,利用梯度阈值和亮度阈值分割天空区域。其次,采用改进的暗通道先验和四叉树细分法估计大气光值。最后,针对天空区域和非天空区域采用不同的透射率估计方法,对于天空区域采用亮通道先验方法,对于非天空区域提出了线性雾气浓度估计模型。结合像素点概率分布得到透射率并采用引导滤波方法进行边缘细化,利用大气散射模型获得最终复原图像。实验结果表明,去雾后的图像在主观和客观质量评价方面表现良好,所提出的算法能够使天空区域恢复自然,去雾更加彻底,增加了图像细节的清晰度。本文算法的运行速率与当前主流算法相当,针对不同有雾场景去雾表现的稳定性优于其它算法。

关键词：图像去雾天空分割大气光估计雾气浓度估计透射率估计

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水产养殖中抗生素使用存在问题及改进措施

畜牧兽医科学（电子版） 2020年第15期 149-150页

作者：韩昊男同济大学上海200092

水产养殖为人类提供安全、可靠、经济的食品,已成为世界上蓬勃发展的产业,而抗生素在水产养殖中被广泛用于疾病的防治。水产养殖中使用的抗生素80%以上最终残留在水环境中,对细菌的选择性压力增大,通过突变或水平基因转移引起细菌对抗... 详细信息

水产养殖为人类提供安全、可靠、经济的食品,已成为世界上蓬勃发展的产业,而抗生素在水产养殖中被广泛用于疾病的防治。水产养殖中使用的抗生素80%以上最终残留在水环境中,对细菌的选择性压力增大,通过突变或水平基因转移引起细菌对抗生素的耐药性,进而改变水环境的生物多样性。该文主要论述抗生素在水产养殖中的浓度、分布、存在问题及耐药性,并制定相应的改进措施,以减少抗生素在水产养殖系统中的使用。

关键词：抗生素水产养殖风险措施

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不锈钢等离子切割工艺技术发展

机械制造文摘（焊接分册） 2016年第4期 20-23页

作者：张永林韩永馗于浩楠王君宇韩宇机械科学研究院哈尔滨焊接研究所黑龙江哈尔滨150028

等离子切割可切割材料多、应用领域广,是国内外普遍关注和研究的主流热切割技术,是切割不锈钢最重要的工艺方法;文中主要叙述等离子切割技术发展过程,总结了等离子切割质量与工艺参数的关系,采集了几种典型厚度不锈钢主要切割工艺参数。

关键词：等离子切割发展切割工艺工艺参数

贺兰山矿区生态修复的乡土植物资源适生性验证与开发价值评价

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宁夏农林科技 2024年第10期65卷 34-38,58页

作者：韩浩南袁旭杰刘慧丽刘秉儒北方民族大学生物科学与工程学院宁夏银川750021 黄河流域农牧交错区生态保护国家民委重点实验室宁夏银川750021

矿山生态环境修复是当前恢复生态学领域关注的热点之一,在植被恢复方面乡土植物因较高的生态适应性和较低维护成本受到重视,但是乡土植物资源开发利用与产业化研究明显滞后。在贺兰山矿区对27个具有生态修复价值的乡土植物开展了大规模... 详细信息

矿山生态环境修复是当前恢复生态学领域关注的热点之一,在植被恢复方面乡土植物因较高的生态适应性和较低维护成本受到重视,但是乡土植物资源开发利用与产业化研究明显滞后。在贺兰山矿区对27个具有生态修复价值的乡土植物开展了大规模种植试验,对其适生性进行验证,并对其开发价值进行综合评价。结果表明:受土质、热量、干旱与盐碱等条件制约,用于贺兰山矿区的乡土植物种类选择范围较小;草本乡土植物白茎盐生草、猪毛菜、沙蒿等适生乡土植物具有先锋植物特征,属于r对策植物,生活史短、种子结实量大,对矿区生境适应性强,构建的人工植被在雨养条件下部分区域植被盖度可达80%;灌木乡土植物酸枣和红砂在贺兰山浅山地带和洪积扇占优势,但在高海拔地区因所需温度条件不足,无法用于较高海拔的贺兰山矿区修复。按照物种材料、种植方式、出苗/生长状况、种子可获得性、商品化价值等指标综合评价,应重点推广的乡土灌草植物有灰榆、沙冬青、蒙古扁桃、柽柳、白茎盐生草、猪毛菜、沙蒿、沙打旺等。上述乡土植物在矿渣土中出苗率或成活率高,可商品化/产业化程度也较高,特别适合作为北方旱区矿山植被恢复的优先选择物种。

关键词：贺兰山矿区适生性乡土植物开发价值

Carbon-coated Ni-Co alloy catalysts:preparation and performance for in-situ aqueous phase hydrodeoxygenation of methyl palmitate to hydrocarbons using methanol as the hydrogen donor

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Frontiers of Chemical Science and Engineering 2022年第4期16卷 443-460页

作者： Yinteng Shi Lin Ai haonan Shi Xiaoyu Gu Yujun han Jixiang Chen Tianjin Key Laboratory of Applied Catalysis Science and Technology Department of Catalysis Science and EngineeringSchool of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China

Carbon-coated Ni,Co and Ni-Co alloy catalysts were prepared by the carbonization of the metal doped resorcinol-formaldehyde resins synthesized by the one-pot extended Stöber *** was found that the introduction of Co remarkably reduced the carbon microsphere *** metallic Ni,Co,and Ni-Co alloy particles(mainly 10-12 nm)were uniformly distributed in carbon microspheres.A charge transfer from Ni to Co appeared in the Ni-Co *** with those of metallic Ni and Co,the d-band center of the Ni-Co alloy shifted away from and toward the Fermi level,*** the in-situ aqueous phase hydrodeoxygenation of methyl palmitate with methanol as the hydrogen donor at 330℃,the decarbonylation/decarboxylation pathway dominated on all *** Ni-Co@C catalysts gave higher activity than the Ni@C and Co@C catalysts,and the yields of n-pentadecane and n-C6-n-C16 reached 71.6%and 92.6%,*** excellent performance of Ni-Co@C is attributed to the electronic interactions between Ni and Co and the small carbon *** to the confinement effect of carbon,the metal particles showed high resistance to sintering under harsh hydrothermal *** deactivation is due to the carbonaceous deposition,and the regeneration with CO_(2) recovered the catalyst reactivity.

关键词： Stöber method carbon-coated Ni-Co alloy in-situ hydrodeoxygenation methyl palmitate decarbonylation decarboxylation