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继代培养球孢白僵菌对斜纹夜蛾幼虫毒力和产孢量的影响及菌株复壮研究

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广东农业科学 2024年第2期51卷 92-100页

作者：陈记娟朱碧锋李皓龙 SHAUKAT Ali 吴建辉华南农业大学植物保护学院/广东省农业害虫生物防治工程技术研究中心广东广州510642

【目的】明确球孢白僵菌(Beauveria bassiana)在连续继代培养过程中对斜纹夜蛾(Spodoptera litura)二龄幼虫毒力、产孢量的影响,并进行菌种复壮研究,为进一步利用球孢白僵菌对斜纹夜蛾的生物防治提供参考。【方法】将球孢白僵菌SCAUJH1... 详细信息

【目的】明确球孢白僵菌(Beauveria bassiana)在连续继代培养过程中对斜纹夜蛾(Spodoptera litura)二龄幼虫毒力、产孢量的影响,并进行菌种复壮研究,为进一步利用球孢白僵菌对斜纹夜蛾的生物防治提供参考。【方法】将球孢白僵菌SCAUJH19继代培养10代,得到F_(1)~F_(10)代菌株并测定产孢量。采用浸虫法分别用F_(1)~F_(10)代菌株处理斜纹夜蛾二龄幼虫,测定其对斜纹夜蛾二龄幼虫的毒力;建立模型分析球孢白僵菌继代培养代数、毒力与产孢量的关系及毒力退化规律。采用活虫复壮法对F_(4)、F_(6)、F_(8)、F_(10)代菌株进行复壮,比较复壮前后对斜纹夜蛾二龄幼虫的毒力效果,并测定产孢量。【结果】球孢白僵菌F_(1)~F_(5)代菌株对二龄斜纹夜蛾幼虫毒力无显著差异,处理7 d时校正死亡率为42.44%~45.22%,而F_(6)、F_(8)、F_(10)代菌株对二龄斜纹夜蛾幼虫的校正死亡率分别降至37.00%、22.94%、12.17%。F_(1)~F_(5)代菌株产孢量为9.65~10.43×10^(8)CFU/mL,F_(6)、F_(8)、F_(10)代菌株产孢量分别降至7.62×10^(8)、5.14×10^(8)、4.69×10^(8)CFU/mL。建立模型发现,菌株继代培养到F_(5)代后,产孢量随培养代数呈现指数下降,且产孢量与毒力存在一定的正相关关系,继代培养代数较少的菌株产孢量较高,对斜纹夜蛾二龄幼虫的致病力较强。通过活虫复壮法对F_(4)、F_(6)、F_(8)、F_(10)代菌株进行复壮后,菌株对斜纹夜蛾二龄幼虫的校正死亡率均>50%,且均高于原始菌株的最高致死率,复壮后菌株的产孢量均高于复壮前的产孢量。【结论】球孢白僵菌在继代培养4~5代后,产孢量逐代下降,对斜纹夜蛾二龄幼虫的毒力也逐代降低,需进行菌种复壮;采用活虫复壮法可将继代后的球孢白僵菌恢复或提高至原有特性;同一菌株的产孢量可作为评价其毒力的参考指标。

关键词：球孢白僵菌斜纹夜蛾二龄幼虫复壮毒力产孢量校正死亡率

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In situ crosslinking of polyoxometalate-polymer nanocomposites for robust high-temperature proton exchange membranes

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Chinese Chemical Letters 2023年第2期34卷 495-499页

作者： Gang Wang Jialin li Haibin li Haibo He liang Zhai Xiang li Tingting li Chengji Zhao lixin Wu haolong li State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China Key Laboratory of High Performance Plastics Ministry of EducationCollege of ChemistryJilin UniversityChangchun 130012China

The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between the proton conductivity and the mechanical performance of these materials. Here,we report an effective strategy to fabricate robust high-temperature PEMs based on the in situ electrostatic crosslinking of polyoxometalates and polymers. A comb copolymer poly(ether-ether-ketone)-grafted-poly(2-ethyl-2-oxazoline)(PGE) with transformable side chains was synthesized and complexed with H_(3)PW_(12)O_(40)(PW) by electrostatic self-assembly, forming PGE/PW nanocomposite membranes with bicontinuous nanostructures. After a subsequent PA-treatment of these membranes, high-temperature PEMs of PGE/PW/PA ternary nanocomposites were obtained, in which the in situ electrostatic crosslinking effect between PW and PGE side chains was generated in the hydrophilic domains of the bicontinuous structures. The microphase separation structure and the electrostatic crosslinking feature endow the PGE/PW/PA membranes with excellent anhydrous proton conductive ability while retaining high mechanical performance. The membranes show a high proton conductivity of 42.5 m S/cm at 150 ℃ and a high tensile strength of 13 MPa. Our strategy can pave a new route based on electrostatic control to design nanostructured polymer electrolytes.

关键词： Polyoxometalates Comb copolymers Self-assembly In situ crosslinking Proton exchange membranes

Inactivated SARS-CoV-2 booster vaccine enhanced immune responses in patients with chronic liver diseases

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Virologica Sinica 2023年第5期38卷 723-734页

作者： Yongmei liu Jianhua Lu Haoting Zhan Wenfang Yuan Xiaomeng li Haiyan Kang haolong li Yongliang Chen linlin Cheng Xingli Sun Haojie Zheng Wei Wang Erhei Dai Yongzhe li Department of Clinical Laboratory State Key Laboratory of ComplexSevere and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing100730China Division of liver Diseases The Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang050021China Department of Clinical Laboratory The Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang050021China Department of Clinical Laboratory Peking University People's HospitalBeijing100035China

Chronic liver disease(CLD)entails elevated risk of COVID-19 severity and *** effectiveness of the booster dose of inactivated SARS-CoV-2 vaccine in stimulating antibody response in CLD patients is ***,we conducted a cross-sectional study involving 237 adult CLD patients and 170 healthy controls(HC)to analyze neutralizing antibodies(NAbs)against SARS-CoV-2 prototype and BA.4/5 variant,anti-receptor binding domain(RBD)IgG,and total anti-SARS-CoV-2 *** levels of the total anti-SARS-CoV-2 antibodies,anti-RBD IgG and inhibition efficacy of NAbs were significantly elevated in CLD patients after the booster dose compared with the pre-booster dose,but were relatively lower than those of *** humoral responses decreased over time after booster *** neutralization efficiency of the serum against BA.4/5 increased but remained below the inhibition *** four SARS-CoV-2 antibodies,including total anti-SARS-CoV-2 antibodies,anti-RBD IgG and NAbs against prototype and BA.4/5,were lower in patients with severe CLD than those with non-severe *** booster shot,age and time after the last vaccine were the risk factors for seropositivity of NAb against BA.4/5 in CLD ***,white blood cell counts and hepatitis B core antibodies were the protective factors,and severe liver disease was the risk factor associated with seropositivity of total anti-SARS-CoV-2 ***,our data uncovered that antibody responses were improved in CLD patients and peaked at 120 days after the booster *** antibodies excepting total anti-SARS-CoV-2 antibodies declined after *** patients exhibited impaired immunologic responses to vaccination and weakened NAbs against BA.4/5,which hindered the protective effect of the booster shot against Omicron *** immune responses should be further evaluated to determine the optimal vaccine regimen for CLD patients.

关键词： Chronic liver disease(CLD) SARS-CoV-2 inactivated vaccines Booster vaccination Antibody response Immune response

Nanostructured Polymer Composite Electrolytes with Self-Assembled Polyoxometalate Networks for Proton Conduction

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CCS Chemistry 2022年第1期4卷 151-161页

作者： Gang Wang Jialin li lichao Shang Haibo He Tingting Cui Shengchao Chai Chengji Zhao lixin Wu haolong li State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012 Key Laboratory of High Performance Plastics Ministry of EducationCollege of ChemistryJilin UniversityChangchun 130012

The key challenge for the use of polymer electrolytes is to realize a high ionic conductivity without scarifying their mechanical ***,we report a facile strategy to prepare a nanostructured polymer electrolyte with both high proton conductivity and high modulus,based on the electrostatic self-assembly of polyoxometalate cluster H_(3)PW_(12)O_(40)(PW)and comb copolymer poly(ether-etherketone)-grafted-poly(vinyl pyrrolidone)(PEEK-gPVP).The incorporation of protonic acid PW can enable the PEEK-g-PVP to be highly proton conductive and create flexible composite electrolyte ***,nanoscale phase separation between PEEK domains and PVP/PW domains spontaneously occurs in these membranes,forming a bicontinuous structure with three-dimensional(3D)-connected PW *** to the dual role of PW networks as both proton transport pathways and mechanical enhancers,these membranes exhibit proton conductivities higher than 30 mS cm^(−1) and modulus over 4 ***,the direct methanol fuel cells equipped with these membranes show good cell *** the wide tunability of comb copolymers and polyoxometalates,this system can be extended to develop a variety of functional electrolyte materials,for example,the lithium-ion conductive electrolytes by using polyoxometalatebased lithium salts,which provides a promising platform to explore versatile electrolyte materials for energy and electronic applications.

关键词： self-assembly polyoxometalates polymer electrolytes nanocomposites proton conduction

Fluorine-Doped High-Performance li_(6)PS_(5)Cl Electrolyte by lithium Fluoride Nanoparticles for All-Solid-State lithium-Metal Batteries

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Transactions of Tianjin University 2024年第3期30卷 250-261页

作者： Xiaorou Cao Shijie Xu Yuzhe Zhang Xiaohu Hu Yifan Yan Yanru Wang Haoran Qian Jiakai Wang haolong Chang Fangyi Cheng Yongan Yang Institute of Molecular Plus School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai UniversityTianjin 300071China

All-solid-state lithium-metal batteries(ASSLMBs)are widely considered as the ultimately advanced lithium batteries owing to their improved energy density and enhanced safety *** various solid electrolytes,sulfide solid electrolyte(SSE)li_(6)PS_(5)Cl has garnered significant ***,its application is limited by its poor cyclability and low critical current density(CCD).In this study,we introduce a novel approach to enhance the performance of li_(6)PS_(5)Cl by doping it with fluorine,using lithium fluoride nanoparticles(liFs)as the doping *** F-doped electrolyte li_(6)PS_(5)Cl-0.2liF(nano)shows a doubled CCD,from 0.5 to 1.0 mA/cm^(2) without compromising the ionic conductivity;in fact,conductivity is enhanced from 2.82 to 3.30 mS/cm,contrary to the typical performance decline seen in conventionally doped li_(6)PS_(5)Cl *** symmetric li|SSE|li cells,the lifetime of li_(6)PS_(5)Cl-0.2liF(nano)is 4 times longer than that of li_(6)PS_(5)Cl,achieving 1500 h vs.371 h under a charging/discharging current density of 0.2 mA/cm^(2).In li|SSE|liNbO_(3)@NCM721 full cells,which are tested under a cycling rate of 0.1 C at 30℃,the lifetime of li_(6)PS_(5)Cl-0.2liF(nano)is four times that of li_(6)PS_(5)Cl,reaching 100 cycles vs.26 ***,the doping of nano-liF off ers a promising approach to developing high-performance li_(6)PS_(5)Cl for ASSLMBs.

关键词： Sulfide solid electrolyte All-solid-state lithium batteries li_(6)PS_(5)Cl lithium fluoride F-doping

白藜芦醇改善青春期酒精暴露导致的成年期大鼠认知功能损害

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中国细胞生物学学报 2024年第3期46卷 436-444页

作者：贾文歌刘媛王心雨李昊隆曾令宇杨一帆李晨晨陈红云赵斌魏来新乡医学院法医学院新乡市法医毒理重点实验室新乡453003

该文旨在研究白藜芦醇(RSV)对青春期间歇性酒精(AIE)暴露大鼠认知功能的影响及潜在的机制。通过25%酒精灌胃建立AIE暴露大鼠模型,并使用RSV进行干预。旷场实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠进入中心区域的次数(P0.05)。Morri... 详细信息

该文旨在研究白藜芦醇(RSV)对青春期间歇性酒精(AIE)暴露大鼠认知功能的影响及潜在的机制。通过25%酒精灌胃建立AIE暴露大鼠模型,并使用RSV进行干预。旷场实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠进入中心区域的次数(P0.05)。Morris水迷宫实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠测试期逃避潜伏期增加(P<0.01)、穿越目标平台次数明显减少(P<0.05)。而RSV治疗后,Eth+RSV组大鼠较Eth+Veh组测试期逃避潜伏期降低(P<0.05)、穿越目标平台次数明显增多(P<0.05)。尼氏染色结果显示,与Con+Veh组相比,Eth+Veh组大鼠内侧前额叶皮层(mPFC)神经元数量显著减少(P<0.001)。与Eth+Veh组相比,Eth+RSV组大鼠mPFC神经元尼氏体数量显著增多(P<0.01)。Western blot结果显示,与Con+Veh组相比,Eth+Veh组大鼠mPFC内SIRT1(P<0.05)、BDNF(P<0.05)及PSD95(P<0.05)蛋白表达水平显著降低。与Eth+Veh组相比,Eth+RSV组大鼠mPFC内SIRT1(P<0.01)、BDNF(P<0.05)及PSD95(P<0.05)蛋白表达水平显著增高。该研究结果表明,RSV能够改善AIE导致的大鼠认知功能损害,并增加mPFC内神经元尼氏体数量,这种改善作用可能与mPFC中SIRT1、BDNF及PSD95的表达水平增加相关。

关键词：白藜芦醇青春期间歇性酒精暴露认知功能沉默信息调节因子1 脑源性神经营养因子突触后密度蛋白95

Numerical simulation of particle flow behavior in a screw conveyor using the discrete element method

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Particuology 2019年第2期17卷 137-148页

作者： Shuyan Wang haolong li Ruichao Tian Ruichen Wang Xu Wang Qiji Sun jiawei Fan School of Petroleum Engineering Northeast Petroleum University Daqing 163318 China

Particle motion in a screw conveyor was simulated w让h the discrete element method. The particle flow behavior and transport processes at different screw rotating speeds and filling levels were investigated in this study. The spatial distributions of particle velocity were predicted. The predicted mass flow rate increased w让h increasing screw rotating speed and filling level. The contact forces and granular temperatures of particles were also calculated. The simulation results showed that the translational granular particle temperatures were higher than the rotational granular particle temperatures. In add让ion, the configurational temperatures of particles were calculated from simulated instantaneous particle overlaps, and results indicated that deformation of elastic particles contributed to the rate of energy dissipation. Good agreement between the numerical simulation and experimental results was achieved in this study.

关键词： Screw conveyor Discrete element method Granular temperature Simulation

Self-assembled lamellar nanochannels in polyoxometalate-polymer nanocomposites for proton conduction

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Chinese Chemical Letters 2021年第6期32卷 2013-2016页

作者： Haibo He Gang Wang Shengchao Chai Xiang li liang Zhai lixin Wu haolong li State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China

The construction of nanostructured ion-transport channels is highly desirable in the design of advanced electrolyte materials,as it can enhance ion conductivity by offering short ion-transport *** this work,we present a supramolecular strategy to fabricate a nanocomposite electrolyte containing highly ordered lamellar proton-conducting nanochannels,by the electrostatic self-assembly of a polyoxometalate H_(3)PW1_(2)O_(4)O(PW)and a comb copolymer poly(4-methlstyrene)-graft-poly(N-vinyl pyrrolidone).PW can effectively regulate the self-assembling order of polymer moieties to form a large-ra nge lamellar structure,meanwhile,introducing protons into the nanoscale lamellar domains to build proton transport ***,the rigid PW clusters contribute a remarkable mechanical reinforcement to the *** lamellar nanocomposite exhibits a conductivity of 4.3×10^(-4)S/cm and a storage modulus of 1.1×10^(7)Pa at room *** study provides a new strategy to construct nanostructured ion-conductive pathways in electrolyte materials.

关键词： Self-assembly Lamellar nanochannels Nanocomposite electrolytes Comb copolymers Polyoxometalates

Effects of helium massive gas injection level on disruption mitigation on EAST

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Plasma Science and Technology 2021年第7期23卷 31-38页

作者： Abdullah ZAFAR Ping ZHU Ahmad Ali Shiyong ZENG haolong li CAS Key Laboratory of Geospace Environment and Department of Engineering and Applied Physics University of Science and Technology of ChinaHefei 230026Peopleʼs Republic of China International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics State Key Laboratory of Advanced Electromagnetic Engineering and TechnologySchool of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074Peopleʼs Republic of China Department of Engineering Physics University of Wisconsin-MadisonMadisonWI 53706United States of America Pakistan Tokamak Plasma Research Institute Islamabad 3329Pakistan Department of Plasma Physics and Fusion Engineering University of Science and Technology of ChinaHefei 230026People’s Republic of China

In this study,NIMROD simulations are performed to investigate the effects of massive helium gas injection level on the induced disruption on EAST *** is demonstrated in simulations that two different scenarios of plasma cooling(complete cooling and partial cooling)take place for different amounts of injected *** the impurity injection above a critical level,a single MHD activity is able to induce a complete core temperature *** impurity injection below the critical level,a series of multiple minor disruptions occur before the complete thermal quench.

关键词： massive gas injection MHD instabilities major disruption minor disruption