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A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement

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Molecular Plant 2023年第2期16卷 432-451页

作者： Xu Zhang Haiyan Wang haojie sun Yingbo Li Yilong Feng Chengzhi Jiao Mengli Li Xinying Song Tong Wang Zongkuan Wang Chunxia Yuan Li sun Ruju Lu Wenli Zhang Jin Xiao Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingJiangsu 210095China Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai 201106China

Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.In this study,we generated a doubled-haploid line,91C43^(DH),using microspore culture and obtained a 4.05-GB high-quality,chromosome-scale genome assembly for D.villosum.The assembly contains39727 high-confidence genes,and 85.31% of the sequences are repetitive.Two reciprocal translocation events were detected,and 7VS-4VL is a unique translocation in D.villosum.The prolamin seed storage protein-coding genes were found to be duplicated;in particular,the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded.RNA sequencing(RNA-seq)analysis indicated that,after Blumeria graminearum f.sp tritici(Bgt)inoculation,there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D.villosum than in Triticum urartu.MNase hypersensitive sequencing(MH-seq)identified two Bgt-inducible MH sites(MHSs),one in the promoter and one in the 3'terminal region of the powdery mildew resistance(Pm)gene NLR1-V.Each site had two subpeaks and they were termed MHS1(MHS1.1/1.2)and MHS2(MHS2.1/2.2).Bgt-inducible MHS2.2 was uniquely present in D.villosum,and MHS1.1 was more inducible in D.villosum than in wheat,suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense.In summary,this study provides a valuable genome resource for functional genomics studies and wheat-D.villosum introgression breeding.The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.

关键词： D.villosum genome crop wild relative genome evolution disease resistance RNA-seq MNase hy-persensitivityassay

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FISH-based “pan” and “core” karyotypes reveal genetic diversification of Roegneria ciliaris

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Journal of Genetics and Genomics 2023年第2期50卷 135-138页

作者： Menghao Cheng Xingyue Li Huimin Cui haojie sun Tianshuo Deng Xinying Song Rongrong Song Tong Wang Zongkuan Wang Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cytogenetics Institute/Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP) Nanjing Agricultural UniversityNanjingJiangsu 210095China

The genus Roegneria was firstly established with Roegneria caucasica C.Koch as the model species.Being the largest genera in the tribe Triticeae(Poaceae),Roegneria includes 130 species mainly distributed in the north temperate zone.There are 70 Roegneria species and 22 variants,which are widely distributed in different regions of China(Lei,2018).The diversified morphology of Roegneria species has caused controversy in their taxonomy.It has been argued whether Roegneria species should be included in the genus Elymus or considered as an independent genus(Lei et al.,2018).The investigation of the cytological and genomic feature would help to clarify the taxonomic placement of Roegneria.

关键词： species Roe temperate

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Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9

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Neuroscience Bulletin 2021年第3期37卷 423-426页

作者： haojie sun Jie Zheng Ming Yi You Wan Neuroscience Research Institute Department of NeurobiologySchool of Basic Medical SciencesPeking UniversityBeijing 100083China Key Laboratory for Neuroscience Ministry of Education/National Health Commission of ChinaPeking UniversityBeijing 100083China Department of Pathophysiology Huazhong University of Science and TechnologyWuhan 430030China

Neuronal ensembles with distinct morphological,biochemical,and functional identities are organized into complex circuits in the mammalian brain,and malfunction of specific neuronal types in different networks contributes to diverse pathological symptoms.Taking memory as an example,specific memories are held in a subset of neurons,referred to as engram cells.

关键词： Mammalian networks organized

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伊犁河谷住宿业空间分布特征及影响因素研究

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地域研究与开发 2024年第2期43卷 105-111页

作者：徐雪婷孙浩捷宋长赢赵鹏凯新疆师范大学地理科学与旅游学院乌鲁木齐830054

利用DBSCAN算法、地理探测器方法剖析伊犁河谷住宿业的空间分布特征及其影响因素。结果表明:(1)伊犁河谷住宿业呈现整体分散与局部聚集的空间分布格局,伊宁市和新源县那拉提旅游风景区为高聚集的核心区域。(2)伊犁河谷住宿业被划分为18... 详细信息

利用DBSCAN算法、地理探测器方法剖析伊犁河谷住宿业的空间分布特征及其影响因素。结果表明:(1)伊犁河谷住宿业呈现整体分散与局部聚集的空间分布格局,伊宁市和新源县那拉提旅游风景区为高聚集的核心区域。(2)伊犁河谷住宿业被划分为18个空间集群,共5个等级,空间上表现为东南—西北走向,且多数住宿业以旅游景点为中心向外辐射分布。(3)不同因素对住宿业的影响程度具有较大的异质性,其中景区、星级酒店、交通设施和人口等为核心影响要素。

关键词：住宿业空间分布影响因素 DBSCAN算法伊犁河谷

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Construction and application of oligo-based FISH karyotype of Haynaldia villosa

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Journal of Genetics and Genomics 2018年第8期45卷 463-466页

作者： haojie sun Jingjing Song Jia Lei Xinying Song Keli Dai Jin Xiao Chunxia Yuan Shengmin An Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics Institute Nanjing Agricultural University/JCIC-MCP

Haynaldia villosa （L.） Schur （syn. Dasypyrurn villosum （L.） Can- dargy）（2n - 14, genome VV）, a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several genes found in H. villosa have been transferred into wheat to improve wheat resistance by the development of alien transloca- tion lines. The seed storage protein loci on chromosome 1V contribute to grain quality （Zhang et al., 2014）.

关键词： VP Construction and application of oligo-based FISH karyotype of Haynaldia villosa GAA

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细胞焦亡在肾缺血再灌注损伤中的研究进展

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中国比较医学杂志 2024年第3期34卷 172-178页

作者：孙豪杰朱俊雷李伟王锁刚河南中医药大学第一附属医院泌尿外科一区郑州450003 河南中医药大学第一临床医学院郑州450003

细胞焦亡是半胱氨酸蛋白酶介导的以线粒体参与、炎性小体组装、质膜穿孔、炎性释放为特征的细胞程序性死亡。作为介导机体炎症反应的重要机制,细胞焦亡在肾缺血再灌注损伤(renal ischemia-reperfusion injury,RIRI)中发挥了关键作用。... 详细信息

细胞焦亡是半胱氨酸蛋白酶介导的以线粒体参与、炎性小体组装、质膜穿孔、炎性释放为特征的细胞程序性死亡。作为介导机体炎症反应的重要机制,细胞焦亡在肾缺血再灌注损伤(renal ischemia-reperfusion injury,RIRI)中发挥了关键作用。本文就近年来细胞焦亡的分子机制、细胞焦亡在RIRI中的作用机制和治疗药物的研究进展进行综述,旨在为RIRI的早期治疗提供理论参考。

关键词：肾移植缺血-再灌注损伤细胞焦亡抑制剂干预手段

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Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat

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The Crop Journal 2020年第4期8卷 676-681页

作者： Jia Lei Jiawen Zhou haojie sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová Jaroslav Dolezel Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjing 210095JiangsuChina Institute of Experimental Botany Czech Academy of SciencesCentre of the Region Hana for Biotechnological and Agricultural ResearchŠlechtitelů3177900 OlomoucCzech Republic

Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat.

关键词： Chromosome identification Haynaldia villosa Oligonucleotide probes Tandem DNA repeats

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The Strategic Transformation and Structure Innovation of Xinjiang Tourism

The Strategic Transformation and Structure Innovation of Xin...

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2011 International Conference on Advanced Materials and Computer Science(ICAMCS 2011)

作者： haojie sun School of Geography Science and Tourism Xinjiang Normal University The Key Subjects of Tourism Management in Xinjiang

Xinjiang is rich in tourist resources, and tourism is embracing a good opportunity, taking a trend of sound development. As the scale of tourism expands and the tourist-guest structure changes, Xinjiang tourism must achieve strategic transformation and structure innovation in such aspects as industrial structure, development mode, industrial function, tourist forms and tourism management to realize a leapfrog development.

关键词： Strategic transformation structure innovation tourism Xinjiang

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A natural variation of an SVP MADS-box transcription factor in Triticum petropavlovskyi leads to its ectopic expression and contributes to elongated glume

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Molecular Plant 2021年第9期14卷 1408-1411页

作者： Jin Xiao Yan Chen Yifan Lu Zhipeng Liu Dongmei Si Tao Xu Li sun Zongkuan Wang Chunxia Yuan haojie sun Xu Zhang Mingxing Wen Luyang Wei Wenli Zhang Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingJiangsu 210095China

Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(Wang et al.,2002;Watanabe and Imamura,2002).The origin of T.petropavlovskyi has been disputed.It is generally accepted that P1pet was introduced from tetraploid polish wheat(T.polonicum)via a hybridization with hexaploid common wheat(T.aestivum)(Akond et al.,2008;Chen et al.,2016).

关键词： Triticum Xinjiang wheat

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内墙乳胶漆抗开裂性能的研究

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上海涂料 2024年第3期62卷 22-26页

作者：张丽刘松洁孙浩杰立邦涂料(中国)有限公司上海201201 黄河三角洲京博化工研究院有限公司山东滨州256599

研究分析了云母粉、滑石粉、煅烧高岭土、成膜助剂对内墙乳胶漆漆膜遮盖性能、开裂性能及其他性能的影响。结果显示,在控制原料成本的前提下,当滑石粉和煅烧高岭土的用量分别为8.0%和12%时,产品能够符合GB/T 9756—2018优等品的遮盖性能... 详细信息

研究分析了云母粉、滑石粉、煅烧高岭土、成膜助剂对内墙乳胶漆漆膜遮盖性能、开裂性能及其他性能的影响。结果显示,在控制原料成本的前提下,当滑石粉和煅烧高岭土的用量分别为8.0%和12%时,产品能够符合GB/T 9756—2018优等品的遮盖性能;与蓬松煅烧高岭土相比较,层状结构的煅烧高岭土更有助于改善漆膜的开裂性能;当成膜助剂的添加量为1.2%时,漆膜的低温成膜性能和耐沾污性均较为理想。通过以上优选解决方案,既可改善内墙乳胶漆的开裂性能,又能控制原料成本,最终提高客户满意度。

关键词：内墙乳胶漆抗开裂对比率低温成膜性

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