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Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets

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Marine Life Science & Technology 2022年第1期4卷 117-126页

作者： Nana Zhao Xianqing Yang Yujin Li haohao Wu Yiping Chen Ruichang Gao feng Xiao Fan Bai Jinlin Wang Zunying Liu Xin Gao Yuanhui Zhao College of Food Science and Engineering Ocean University of ChinaQingdao266003China South China Sea Fisheries Institute Chinese Academy of Fishery SciencesGuangzhou510641China School of Food and Bioengineering Jiangsu UniversityZhenjiang212013China College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Hangzhou Qiandaohu Sturgeon Technology Co. LtdHangzhou310000China

Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (−3 °C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 °C). The sarcoplasmic protein, carbonyl, myofibrillar protein, total sulfhydryl content and the surface hydrophobicity were determined using fluorescence spectrophotometry and SDS-PAGE. Results showed that superchilling might protect myofibrillar proteins from oxidation compared to refrigeration temperatures. The activity of the three cathepsins (B, L, and H) in terms of myofibrillar, mitochondria, lysosomes, and sarcoplasm demonstrated that superchilling can inhibit cathepsins activity in sturgeon and protect its muscle structure. Microscopic observations showed that as the temperature decreased, the gap area of the muscle fibers decreased, and the deformation of cross-sectional slices was gradually reduced. In addition, the freezing rate of ice crystals produced during the freezing process influenced the muscle structure, texture, and sensory attributes. Superchilled sturgeon fillets showed good hardness, chewiness, and water retention. In conclusion, superchilling technology shows promise for its ability to extend the shelf life while maintaining the texture and sensory attributes of fresh sturgeon fillets.

关键词： Sturgeon Superchilling Protein oxidation Cathepsin Microstructure

Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize

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Molecular Plant 2022年第10期15卷 1558-1574页

作者： Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu haohao Wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong feng Qin Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBejing 100193China School of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaodingHebei071002China

While crop yields have historically increased,drought resistance has become a major concern in the context of global climate *** trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain *** genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal ***1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable *** contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought ***,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern ***,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased ***,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change.

关键词： ZmSRO1d stomatal ROS drought resistance yield maize

A movement-assisted software-defined sensor network with NFV support

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Journal of Southeast University(English Edition) 2018年第2期34卷 156-165页

作者： Yin haohao Ding Cui Yan feng Xia Weiwei Shen Lianfeng National Mobile Communications Research Laboratory Southeast UniversityNanjing 210096China

A flexible and controllable movement-assisted software-defined sensor network(MA-SDSN)based on the software-defined network(SDN)and network function virtualization(NFV)is ***,a three-layer fundamental architecture is proposed to overcome the inherent distributed management and rigidity of the conventional wireless sensor ***,the platform for research and development of MA-SDSN is established,and the dumb node(DN),the software-defined node(SN)and the movement-assisted node(MN)are designed and ***,the southbound application programming interface(API)is designed to provide a series of frames for communication between controllers and sensor *** northbound API is developed and demonstrated overall and in *** functions of the controller are presented including topology discovery,dynamic networking,packet processing,mobility management and *** by the MA-SDSN network model,a Markov chain-based movement-assisted weighted relocation(MMWR)topology control algorithm is proposed to redeploy the MNs based on the node status and *** results and analysis indicate that the proposed algorithm based on the MA-SDSN extends network lifetime with a lower average power consumption.

关键词： software-defined sensor network network function virtualization movement-assisted topology control

低分子量苯乙烯-马来酸酐树脂酯化物的制备与应用

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广州化学 2024年第2期49卷 36-40,I0002页

作者：刘继强胡豪豪张春李贵东徐长安罗啟权冯逸彬胡洋杨卓鸿广东三求光固材料股份有限公司广东佛山528200 华南农业大学材料与能源学院生物基材料与能源教育部重点实验室广东广州510642

采用溶液聚合法合成了多种低分子量苯乙烯-马来酸酐无规共聚物(SMA),然后加入甲基丙烯酸羟乙酯和催化剂,制备了适用于抗蚀抗电镀油墨领域的低成本酯化树脂(SMAH)。探究了不同溶剂、不同单体配比和不同温度条件对SMA分子量的影响。通过... 详细信息

采用溶液聚合法合成了多种低分子量苯乙烯-马来酸酐无规共聚物(SMA),然后加入甲基丙烯酸羟乙酯和催化剂,制备了适用于抗蚀抗电镀油墨领域的低成本酯化树脂(SMAH)。探究了不同溶剂、不同单体配比和不同温度条件对SMA分子量的影响。通过分子量测试表明,SMA的分子量受体系溶剂种类、单体配比和反应温度的影响,并且制备的树脂的最低分子量可达6000 g/mol以下。随后,对应用在油墨中自合成SMAH树脂的各项性能测试表明,阻焊油墨SMAH-3树脂的显影性、附着力和耐热性指标均正常,且其反射率和耐黄变性分别为86.5和0.36。因此,SMAH-3树脂可用于制备阻焊油墨。

关键词：苯乙烯马来酸酐阻焊油墨分子量溶液聚合

一种基于新灵敏度指标的五轴数控机床关键几何误差辨识方法

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仪器仪表学报 2022年第12期43卷 120-128页

作者：陶浩浩陈丰李同杰范晋伟安徽科技学院机械工程学院滁州233100 北京工业大学材料与制造学部北京100124

精度设计是提升机床加工精度的有效途径,而准确辨识关键几何误差并为其合理分配权重是实现精度设计的前提条件,因此,提出了一种识别关键几何误差的灵敏度分析方法。首先基于多体系统理论建立了机床加工误差模型,并基于该模型构建了灵敏... 详细信息

精度设计是提升机床加工精度的有效途径,而准确辨识关键几何误差并为其合理分配权重是实现精度设计的前提条件,因此,提出了一种识别关键几何误差的灵敏度分析方法。首先基于多体系统理论建立了机床加工误差模型,并基于该模型构建了灵敏度分析模型,同时定义了一种新的灵敏度指标。然后,通过仿真分析明确了第10、17、22、24和37项几何误差为关键几何误差,同时实现了对各项几何误差分配权重。最后,通过补偿关键几何误差和全部几何误差的方式对“S”形检测试件进行加工并对比其轮廓度误差,对比结果显示通过两种补偿方式获得的平均轮廓度误差在3条检测线上的差值很小,分别为0.005、0.004和0.006 mm,因此证明了提出的灵敏度分析方法的正确性。

关键词：关键几何误差加工误差五轴数控机床灵敏度分析 “S”形检测试件

突发公共卫生事件档案馆建设模式探析——以新冠肺炎疫情档案馆为例

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档案学通讯 2020年第3期 74-79页

作者：冯湘君卜昊昊南开大学商学院天津300071

突发公共卫生事件档案馆应具备"记录-纪念-记忆"三位一体的社会功能,建成个人与社会的"记录馆"、"纪念馆"和"记忆馆"。基于该功能定位,新冠肺炎疫情档案馆应以国家综合档案馆、企事业单位档案机构和社群档案馆等多方主体共同参与的联动... 详细信息

突发公共卫生事件档案馆应具备"记录-纪念-记忆"三位一体的社会功能,建成个人与社会的"记录馆"、"纪念馆"和"记忆馆"。基于该功能定位,新冠肺炎疫情档案馆应以国家综合档案馆、企事业单位档案机构和社群档案馆等多方主体共同参与的联动模式,建成全国联动的专题数字档案馆,以完善突发公共卫生事件记忆库。此外,还需要社会治理理念的指导、馆藏资源的保障、信息技术的支持等多种实现条件。

关键词：突发公共卫生事件新型冠状病毒社群档案馆

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固有周期下阻尼板对半潜式平台阻尼的影响

哈尔滨工程大学学报 2018年第5期39卷 831-836页

作者：韩端锋郝浩浩高良田刘恬刘峰哈尔滨工程大学船舶工程学院黑龙江哈尔滨150001

为了研究半潜式平台在固有周期下的自由衰减运动,本文基于重叠网格技术的计算流体力学(CFD)方法对半潜式平台垂荡、横摇和纵摇自由衰减运动进行数值模拟。利用该方法分别研究了不同初始衰减位置下,阻尼板宽度和厚度对半潜式平台垂荡、... 详细信息

为了研究半潜式平台在固有周期下的自由衰减运动,本文基于重叠网格技术的计算流体力学(CFD)方法对半潜式平台垂荡、横摇和纵摇自由衰减运动进行数值模拟。利用该方法分别研究了不同初始衰减位置下,阻尼板宽度和厚度对半潜式平台垂荡、横摇和纵摇阻尼的影响规律,并从流场角度分析了阻尼板的作用机理,通过与实验数据对比,验证该方法的准确性。研究结果表明:平台运动幅度越大,阻尼板起到的抑制作用越强,阻尼板边缘处的流动分离是平台阻尼增加的直接原因;阻尼板宽度与平台垂荡、横摇及纵摇阻尼呈正相关,而阻尼板厚度与其呈负相关。

关键词：半潜式平台阻尼板宽度厚度自由衰减运动阻尼流动分离重叠网格

A metabolic acidity-activatable calcium phosphate probe with fluorescence signal amplification capabilities for non-invasive imaging of tumor malignancy

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Science Bulletin 2022年第3期67卷 288-298页

作者： Qian Jia Ruili Zhang Yongdong Wang haohao Yan Zheng Li Yanbin feng Yu Ji Zuo Yang Yang Yang Kanyi Pu Zhongliang Wang Laboratory of Molecular Imaging and Translational Medicine(MITM) Engineering Research Center of Molecular&NeuroimagingMinistry of EducationSchool of Life Science and TechnologyXidian UniversityXi’an 710126China Academy of Advanced Interdisciplinary Research Xidian UniversityXi’an 710071China Department of Thoracic Surgery Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghai 200433China School of Materials Science and Engineering Tongji UniversityShanghai 201804China School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore 637457Singapore

Dysregulated energy metabolism has recently been recognized as an emerging hallmark of *** cells,which are characterized by abnormal glycolysis,exhibit a lower extracellular pH(6.5–7.0)than nor-mal tissues(7.2–7.4),providing a promising target for tumor-specific imaging and ***,most pH-sensitive materials are unable to distinguish such a subtle pH difference owing to their wide and continuous pH-responsive *** this study,we developed an efficient strategy for the fabrication of a tumor metabolic acidity-activatable calcium phosphate(CaP)fluorescent probe(termed MACaP9).Unlike traditional CaP-based biomedical nanomaterials,which only work within more acidic organelles,such as endosomes and lysosomes(pH 4.0–6.0),MACaP9 could not only specifically respond to the tumor extra-cellular pH but also rapidly convert pH variations into a distinct fluorescence signal to visually dis-tinguish tumor from normal *** superior sensitivity and specificity of MACaP9 enabled high-contrast visualization of a broad range of tumors,as well as small tumor lesions.

关键词： Calcium phosphate Activatable probe Cancer imaging Tumor microenvironment Tumor metabolism

加强科普能力建设,推动科普技术普及——国土资源部地下水科学与工程科普基地建设成果及近期规划

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地质论评 2017年第5期63卷 1306-1308页

作者：张亚哲崔浩浩冯欣中国地质科学院水文地质环境地质研究所石家庄050061

华北是严重缺水地区，建设地下水科学与工程科普基地对于提高公众科学用水意识具有十分重要的现实意义和长远意义。地下水科学与工程科普基地在地下水研究领域科研特色明显，具有开展科普活动的基础条件和设施，该基地具备比较完备的展... 详细信息

华北是严重缺水地区，建设地下水科学与工程科普基地对于提高公众科学用水意识具有十分重要的现实意义和长远意义。地下水科学与工程科普基地在地下水研究领域科研特色明显，具有开展科普活动的基础条件和设施，该基地具备比较完备的展示技术以及职业的科普团队，已符合《国土资源科普基地标准》（科研实验类）的有关要求，2011年被国土资源部命名为第二批国土资源科普基地。被批为科普基地以来，结合自身特点以及公众和社会需求，编制实施科普工作规划计划，加强科普能力建设，积极开展持续有效的科普活动，传播国土资源知识，提升全民科学素养，在国土资源领域科普工作中发挥着重要的作用。

关键词：国土资源科普基地建设规划