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Journal of Ocean University of China 2023年第6期22卷 1613-1620页

作者： GUO Wei LI Shiyang JING Zhehua wu haohao School of Pharmacy Binzhou Medical UniversityYantai 264003China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China

Natural nano-hydroxyapatite(HA)was extracted from Pacific cod(Gadus macrocephalus)bone with a thermostable col-lagenolytic protease in the present *** for the enzymatic reaction were optimized to be 60℃and pH 7.0,and a desir-able extraction efficiency was achieved by using the crude collagenolytic *** light scattering,transmission electron microscopy and energy-dispersive X-ray analysis revealed that nano-HA are anionic spherical(about 110nm)particles mainly com-prised of calcium and phosphorus at an approximate ratio of 5:*** evaluated with the mouse ex vivo intestinal segments,the extracted nano-HA displayed comparable level of intestinal bioavailability to the positive control CaCl_(2).By treating with inhibitors(NaN3,ami-loride)and low temperature(4℃),clathrin-mediated endocytosis was assumed to involve the intestinal absorption of ***-all,the application of thermostable collagenolytic protease is proved to be a promising alternative method for nano-HA extraction from natural resource with improved ecological and biological value.

关键词： nano-hydroxyapatite thermostable collagenolytic protease Pacific cod(Gadus macrocephalus)bone intestinal bio-availability

Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens

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Animal Nutrition 2017年第2期3卷 109-113页

作者： Zhenhua Gao haohao wu Lin Shi Xiaohui Zhang Ran Sheng Fuquan Yin Ravi Gooneratne College of Agriculture Guangdong Ocean University Department of Wine.Food and Molecular Biosciences Lincoln University

To study the influence of different levels of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d Arbor Acres(AA) broilers, a total of 800 one-day-old healthy broilers were randomly divided into 5 groups with 4 replicates per group and 40 broilers per replicate. Broilers were fed a basic diet(group 1) which acted as the control group, and 4 other groups(2 to 5) were fed the basal diet with B. subtilis added at concentrations of 100, 150, 200 and 250 mg/kg, respectively for 42 days. The results showed as follow: the average daily gain(ADG) of group 4 was significantly higher than(P 0.05). The feed to gain ratio(F/G) of all the experimental groups was lower than that of the control and the difference was significant in group 4(P 0.05), crude fat(P > 0.05), dry matter(P > 0.05) and organic matter(P < 0.05). B. subtilis decreased the Escherichia coli and Salmonella populations in the cecum. This shows that adding B. subtilis to the broiler diet can improve the growth performance, increase feed efficiency, regulate serum index and reduce harmful bacteria in the intestinal tract. Based on our study, it could be recommended that addition of B. subtilis at 200 mg/kg could improve the growth performance of broilers.

关键词： Bacillus subtilis AA broilers Growth performance Intestinal microflora

Effect of vegetation type, wetting intensity, and nitrogen supply on external carbon stimulated heterotrophic respiration and microbial biomass carbon in forest soils

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Science China Earth Sciences 2015年第6期58卷 1446-1456页

作者： wu haohao XU XingKai DUAN CunTao LI TuanSheng CHENG WeiGuo State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of Sciences College of Earth Science University of Chinese Academy of Sciences College of Earth Science and Resources Chang'an University Faculty of Agriculture Yamagata University

By using packed soil-core incubation experiments, we have studied stimulating effects of addition of external carbon （C）（glu- cose, 6.4 g C m 2） on heterotrophic respiration and microbial biomass C of a mature broadleaf and Korean pine mixed forest （BKPF） and an adjacent white birch forest （WBF） soil under different wetting intensities （55% and 80% WFPS, water-filled pore space） and nitrogen （N） supply （NH4C1 and KNO3, 4.5 g N m-e） conditions. The results showed that for the control, the cumulative carbon dioxide （CO2） flux from WBF soil during the 15-day incubation ranged from 5.44 to 5.82 g CO2-C m-2, which was significantly larger than that from BKPF soil （2.86 to 3.36 g CO2-C m 2）. With increasing wetting intensity, the cumulative CO2 flux from the control was decreased for the WBF soil, whereas an increase in the CO2 flux was observed in the BKPF soil （P 〈 0.05）. The addition of NH4C1 or KNO3 alone significantly reduced the cumulative CO2 fluxes by 9.2%-21.6 % from the two soils, especially from WBF soil at low wetting intensity. The addition of glucose alone significantly increased soil heterotrophic respiration, microbial biomass C （MBC）, and microbial metabolic quotient. The glucose-induced cumulative CO2 fluxes and soil MBC during the incubation ranged from 8.7 to 11.7 g CO2-C m-2 and from 7.4 to 23.9 g C m-2, which are larger than the dose of added C. Hence, the addition of external carbon can increase the decomposition of soil native organic C. The glucose-induced average and maximum rates of CO2 fluxes during the incubation were significantly in- fluenced by wetting intensity （WI） and vegetation type （VT）, and by WIxVT, NH4ClxVT and WIxVTxNH4C1 （P〈0.05）. The addition of NH4C1, instead of KNO3, significantly decreased the glucose-induced MBC of WBF soil （P〈0.05）, whereas adding NH4C1 and KNO3 both significantly increased the glucose-induced MBC of BKPF soil at high moisture （P〈0.05）. According to the differences in soil labile C pools, MBC and CO2 fluxes

关键词： dissolved organic carbon forest soil glucose heterotrophic respiration microbial biomass carbon nitrogen supply stimulating effect

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Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets

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Marine Life Science & Technology 2022年第1期4卷 117-126页

作者： Nana Zhao Xianqing Yang Yujin Li haohao wu Yiping Chen Ruichang Gao Feng Xiao Fan Bai Jinlin Wang Zunying Liu Xin Gao Yuanhui Zhao College of Food Science and Engineering Ocean University of ChinaQingdao266003China South China Sea Fisheries Institute Chinese Academy of Fishery SciencesGuangzhou510641China School of Food and Bioengineering Jiangsu UniversityZhenjiang212013China College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Hangzhou Qiandaohu Sturgeon Technology Co. LtdHangzhou310000China

Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (−3 °C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 °C). The sarcoplasmic protein, carbonyl, myofibrillar protein, total sulfhydryl content and the surface hydrophobicity were determined using fluorescence spectrophotometry and SDS-PAGE. Results showed that superchilling might protect myofibrillar proteins from oxidation compared to refrigeration temperatures. The activity of the three cathepsins (B, L, and H) in terms of myofibrillar, mitochondria, lysosomes, and sarcoplasm demonstrated that superchilling can inhibit cathepsins activity in sturgeon and protect its muscle structure. Microscopic observations showed that as the temperature decreased, the gap area of the muscle fibers decreased, and the deformation of cross-sectional slices was gradually reduced. In addition, the freezing rate of ice crystals produced during the freezing process influenced the muscle structure, texture, and sensory attributes. Superchilled sturgeon fillets showed good hardness, chewiness, and water retention. In conclusion, superchilling technology shows promise for its ability to extend the shelf life while maintaining the texture and sensory attributes of fresh sturgeon fillets.

关键词： Sturgeon Superchilling Protein oxidation Cathepsin Microstructure

Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize

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Molecular Plant 2022年第10期15卷 1558-1574页

作者： Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu haohao wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong Feng Qin Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBejing 100193China School of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaodingHebei071002China

While crop yields have historically increased,drought resistance has become a major concern in the context of global climate *** trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain *** genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal ***1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable *** contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought ***,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern ***,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased ***,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change.

关键词： ZmSRO1d stomatal ROS drought resistance yield maize

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近距离煤层上覆采空区自然发火治理技术

煤矿安全 2017年第5期48卷 95-97页

作者：伍好好西安科技大学能源学院陕西西安710054

结合白皎煤矿2274工作面掘进及贯通后上覆采空区发生的严重自然发火隐患,分析了隐患产生的原因,确立"灭火+防火"的联合治灾方案,提出了近距离复杂地质构造煤层上覆采空区自然发火综合治理技术。以施工观测钻孔对上覆采空区火灾隐患区域... 详细信息

结合白皎煤矿2274工作面掘进及贯通后上覆采空区发生的严重自然发火隐患,分析了隐患产生的原因,确立"灭火+防火"的联合治灾方案,提出了近距离复杂地质构造煤层上覆采空区自然发火综合治理技术。以施工观测钻孔对上覆采空区火灾隐患区域探查为先导,合理划定隐患范围;采取采空区注液态CO_2灭火降温、巷道喷浆堵漏以及注氮惰化等手段,成功处理上覆采空区自然发火隐患的同时,保障工作面安全回采。

关键词：上覆采空区自然发火复杂地质构造液态CO2 注氮

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基于可变核的月径流改进非参数解集模型研究

水力发电学报 2021年第2期40卷 100-110页

作者：吴昊昊宋松柏西北农林科技大学水利与建筑工程学院西北农林科技大学旱区农业水土工程教育部重点实验室陕西杨凌712100

径流序列模拟可为水资源系统规划和管理提供可靠依据。现行非参数解集模型模拟存在难以保持首尾自相关结构一致性,可能会产生大量负径流值的边界问题等不足。本文应用核密度估计理论,综合考虑年径流量和前期一阶月径流量与模拟月径流量... 详细信息

径流序列模拟可为水资源系统规划和管理提供可靠依据。现行非参数解集模型模拟存在难以保持首尾自相关结构一致性,可能会产生大量负径流值的边界问题等不足。本文应用核密度估计理论,综合考虑年径流量和前期一阶月径流量与模拟月径流量的关系,采用可变核带宽方法减小边界影响,构建了月径流改进非参数解集模型,并将该模型与传统相关解集模型进行对比验证,应用于黄河流域兰州站1932—2010年天然月径流资料。结果表明:改进模型能较好地保持原径流序列的概率分布和统计参数,克服了首尾自相关结构不一致性问题,避免了大量负径流值的产生,用于月径流随机模拟是可行的。

关键词：月径流随机模拟核密度估计改进非参数解集模型可变核相关解集模型

机加工刀具用M2高速钢锻造温度的PID控制优化

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热加工工艺 2022年第15期51卷 99-101页

作者：王晋海吴豪豪包头铁道职业技术学院内蒙古包头014060 北京理工大学材料科学与工程学院北京100081

分别采用常规锻造和PID温度控制锻造方法进行了机加工刀具用M2高速钢的锻造试验,并进行了两种方式锻造试样的显微组织和耐磨损性能对比分析。结果表明:常规锻造时,M2高速钢中的碳化物形态不仅有细小的颗粒状,还有聚集的大块状和少量鱼... 详细信息

分别采用常规锻造和PID温度控制锻造方法进行了机加工刀具用M2高速钢的锻造试验,并进行了两种方式锻造试样的显微组织和耐磨损性能对比分析。结果表明:常规锻造时,M2高速钢中的碳化物形态不仅有细小的颗粒状,还有聚集的大块状和少量鱼骨状。采用PID控制锻造时,试样内部碳化物为呈弥散分布的较为细小的颗粒状。与常规锻造相比,PID控制锻造的M2高速钢显微组织明显细化、组织分布更为均匀;在摩擦磨损10 min后PID控制锻造的试样磨损体积从30×10^(-3)mm^(3)减小到19×10^(-3)mm^(3),减小了11×10^(-3)mm^(3)。采用PID控制优化的锻造温度可以有效改善M2高速钢的内部组织,提高钢的耐磨损性能。

关键词： PID控制优化锻造温度机加工刀具 M2高速钢耐磨损性能

全脑照射对幼鼠海马神经元树突棘形态及密度的影响

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中华放射医学与防护杂志 2021年第9期41卷 641-646页

作者：丁昕吴昊昊冀胜军田野徐州医科大学附属医院放疗科 221000 盐城市第一人民医院放疗科 224006 苏州市立医院放疗科 215002 苏州大学第二附属医院放疗科 215004

目的了解电离辐射对幼鼠海马齿状回(DG)区和CA1区树突棘形态及结构随时间变化的特点,为研究放射性认知功能障碍发生的分子机制提供详尽直接的形态学依据。方法给予21 d龄SD大鼠单次剂量10 Gy全脑照射,观察照射后1、3个月大鼠认知功能改... 详细信息

目的了解电离辐射对幼鼠海马齿状回(DG)区和CA1区树突棘形态及结构随时间变化的特点,为研究放射性认知功能障碍发生的分子机制提供详尽直接的形态学依据。方法给予21 d龄SD大鼠单次剂量10 Gy全脑照射,观察照射后1、3个月大鼠认知功能改变,海马DG区和CA1区中树突棘密度及形态学变化。Western blot检测电离辐射对突触后致密蛋白(PSD95)的影响。结果SD幼鼠在照射后3个月发生明显的学习和记忆力损伤。海马DG区树突棘密度显著降低,照射后1、3个月分别降低了39.06%、29.27%(t=14.96、12.35,P<0.05)。海马CA1区底树突的树突棘密度在照射后1个月降低了33.40%(t=10.39,P<0.05),而在照射后3个月其树突棘密度无明显变化;而CA1区顶树突的树突棘密度在照射后1、3个月均无明显变化。此外,海马DG区和CA1区树突棘形态处于动态变化的过程;突触后PSD95在照后1和3个月分别降低了24.6%和50.5%(t=2.97、9.27,P<0.05)。结论电离辐射后幼鼠海马不同脑区树突棘密度及形态学变化提示PSD95可能通过影响树突棘的结构形态降低突触可塑性,从而参与放射性认知功能障碍的发生。

关键词：电离辐射海马树突棘突触可塑性突触后致密蛋白

Erratum to: Effect of vegetation type, wetting intensity, and nitrogen supply on external carbon stimulated heterotrophic respiration and microbial biomass carbon in forest soils

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Science China Earth Sciences 2017年第7期60卷 1382-1382页