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Monitoring cellular metabolism of 3T3 upon wild type E. coli infection by mapping NADH with FLIM

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Chinese Optics Letters 2010年第10期8卷 931-933页

作者： Tatyana Buryakina Pin-Tzu Su Vladimir Gukassyan Wan-Jr Syu Fu-Jen kao Institute of Biophotonics National Yang-Ming University Institute of Microbiology and Immunology National Yang-Ming University Department of Photonics National Sun Yat-Sen University

Fluorescence lifetime imaging microscopy （FLIM） has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide （NADH）, one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH＇s autofluorescence is detected. The ratio of the short and the long lifetime components＇ relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection.

关键词： Amides Cell culture Phosphorylation Tissue

Optimization of multi-model ensemble forecasting of typhoon waves

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Water Science and Engineering 2016年第1期9卷 52-57页

作者： Shun-qi Pan Yang-ming Fan Jia-ming Chen Chia-chuen kao Hydro-environmental Research Centre School of Engineering Cardiff University Coastal Ocean Monitoring Center National Cheng Kung University Department of Hydraulic and Ocean Engineering National Cheng Kung University

Accurately forecasting ocean waves during typhoon events is extremely important in aiding the mitigation and minimization of their potential damage to the coastal infrastructure, and the protection of coastal communities. However, due to the complex hydrological and meteorological interaction and uncertainties arising from different modeling systems, quantifying the uncertainties and improving the forecasting accuracy of modeled typhoon-induced waves remain challenging. This paper presents a practical approach to optimizing model-ensemble wave heights in an attempt to improve the accuracy of real-time typhoon wave forecasting. A locally weighted learning algorithm is used to obtain the weights for the wave heights computed by the WAVEWATCH III wave model driven by winds from four different weather models （model-ensembles）. The optimized weights are subsequently used to calculate the resulting wave heights from the model-ensembles. The results show that the opti- mization is capable of capturing the different behavioral effects of the different weather models on wave generation. Comparison with the measurements at the selected wave buoy locations shows that the optimized weights, obtained through a training process, can significantly improve the accuracy of the forecasted wave heights over the standard mean values, particularly for typhoon-induced peak waves. The results also indicate that the algorithm is easy to imnlement and practieal for real-time wave forecasting.

关键词： Wave modeling Optimization Forecasting Typhoon waves WAVEWATCH III Locally weighted learning algorithm

Antiarrhythmic drug usage and prostate cancer： a population-based cohort study

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Asian Journal of Andrology 2018年第1期20卷 37-42页

作者： Li-Ting kao Chung-Chien Huang Herng-Ching Lin Chao-Yuan Huan 1Graduate Institute of Life Science National Defense Medical Center Taipei 110 Taiwan China Sleep Research Center Taipei Medical University Hospital Taipei 110 Taiwan China School of Health Care Administration Taipei Medical University Taipei 110 Taiwan China Department of Urology National Taiwan University Hospital National Taiwan University College of Medicine Taipei 110 Taiwan China Department of Urology National Taiwan University Hospital Hsin Chu Branch Hsin Chu City 100 Taiwan China School of Public Health Taipei Medical University Taipei 100 Taiwan China

Even though the relationship between antiarrhythmic drug usage and subsequent prostate cancer （PCa） risk has recently been highlighted, relevant findings in the previous literature are still inconsistent. In addition, very few studies have attempted to investigate the association between sodium channel blockers or potassium channel blockers for arrhythmia and the subsequent PCa risk. Therefore, this cohort study aimed to find the relationship between antiarrhythmic drug usage and the subsequent PCa risk using a population-based dataset. The data used in this study were derived from the Longitudinal Health Insurance Database 2005, Taiwan, China. We respectively identified 9988 sodium channel blocker users, 3663 potassium channel blocker users, 65 966 beta-blocker users, 23 366 calcium channel blockers users, and 7031 digoxin users as the study cohorts. The matched comparison cohorts （one comparison subject for each antiarrhythmic drug user） were selected from the same dataset. Each patient was tracked for a 5-year period to define those who were subsequently diagnosed with PCa. After adjusting for sociodemographic characteristics, comorbidities, and age, Cox proportional hazard regressions found that the hazard ratio （HR） of subsequent PCa for sodium channel blocker users was 1.12 （95% confidence interval [CI]： 0.84-1.50）, for potassium channel blocker users was 0.89 （95% CI. 0.59-1.34）, for beta-blocker users was 1.08 （95% Ch 0.96-1.22）, for calcium channel blocker users was 1.14 （95% Ch 0.95-1.36）, and for digoxin users was 0.89 （95% Ch 0.67-1.18）, compared to their matched nonusers. We concluded that there were no statistical associations between different types of antiarrhvthmic drug usage and subsequent PCa risk.

关键词： antiarrhythmic drugs cancer digoxin ion channel blocker prostate cancer

Transcatheter arterial embolization treatment in patients with hepatocellular carcinoma and risk of pulmonary metastasis

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World Journal of Gastroenterology 2003年第6期9卷 1208-1211页

作者： Shee-Chan Lin Shou-Chuan Shih Chin-Roa kao Sun-Yen Chou Division of Gastroenterology Department of Internal MedicineMackay Memorial HospitalTaipeiTaiwanChina

AIM: To investigate the relationship between transcatheterarterial embolization (TAE) and pulmonary metastasis insubjects with hepatocellular carcinoma (HCC).METHODS: A total of 287 patients with HCC followed upfor more than 1 week were included. 102 patients underwenttranscatheter arterial embolization (TAE group) and 185received conservative treatment(control group). Thepatients' chest x-rays and chest CT scans were examinedfor pulmonary ***: Patients with TAE had a median survival of 19.3months while that of the control group was only 10.0 months(P＜0.05). Pulmonary metastasis occurred in 14 (13.7 %)patients in the TAE group and 14 (7.6 %) patients in the control group, there was no significant difference (P＞0.05).The 1-year, 2-year and 5-year cumulative incidence of pulmonary metastasis was 11.8 %, 17.6 % and 24.0 % in the TAE group and 7.0 %, 13.0 % and 21.7 % in the control group, respectively (P＞0.05). On the univariate analysis,tumor size, abnormal serum alanine aminotransferase levels and heterogeneity on sonography were significantly associated with pulmonary metastasis. However, on the multivariate analysis, only tumor size was significantly predictive of pulmonary ***: TAE is effective on prolonging survival of patients with HCC. It does not significantly increase the risk of pulmonary metastasis. Tumor size is the only significant predictive factor associated with lung metastasis.

关键词：肝细胞癌选择性动脉栓塞治疗肺转移癌危险因素

No increased risk of dementia in patients receiving androgen deprivation therapy for prostate cancer： a 5-year follow-up study

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Asian Journal of Andrology 2017年第4期19卷 414-417页

作者： Li-Ting kao Herng-Ching Lin Shiu-Dong Chung Chao-Yuan Huang Graduate Institute of Life Science National Defense Medical Center Taipei 110 Taiwan China Sleep Research Center Taipei Medical University Hospital Taipei 110 Taiwan China Division of Urology Department of Surgery Far Eastern Memorial Hospital Taipei 110 Taiwan China Graduate Program in Biomedical Informatics College of Informatics Yuan Ze University Chung Li 320 Taiwan China Department of Urology National Taiwan University Hospital College of Medicine National Taiwan University Taipei 110 Taiwan China School of Public Health Taipei Medical University Taipei 110 Taiwan China

Prior studies suggested that the use of androgen deprivation therapy （ADT） in patients with prostate cancer （PC） might cause the impairment of cognitive function which is one of the common symptoms of dementia; however, the association between ADT and cognitive impairment still remains controversial. This retrospective cohort study aimed to investigate the relationship between ADT and subsequent risk of dementia using a population-based dataset, Data for this study were taken from the Taiwan （China） Longitudinal Health Insurance Database 2005. We included 755 PC patients who received ADT in the study cohort and 559 PC patients who did not receive ADT in the comparison cohort. Each patient was individually tracked for a 5-year period to define those who subsequently received a diagnosis of dementia. Results show that the incidence rates of dementia per 100 person-years were 2.35 （95% confidence interval [95% CI]： 1.82-2.98） and 1.85 （95% Ch 1.35-2.48） for PC patients who received ADT and those who did not receive ADT, respectively. The adjusted hazard ratio （HR） for dementia for PC patients who received ADT was 1.21 （95% Ch 0.82-1.78, P = 0,333） compared to those who did not receive ADT. In addition, the adjusted HRs for dementia for PC patients receiving ADT with gonadotropin-releasing hormone （GnRH） agonists and without GnRH agonists were 1.39 （95% Ch 0.80-2.40, P = 0.240） and 1.13 （95% CI： 0.75-1.71, P = 0.564）, respectively, compared to PC patients not receiving ADT. We concluded that there was no difference in the risk of subsequent dementia between PC patients who did and those who did not receive ADT.

关键词： androgen deprivation therapy dementia epidemiology prostate cancer

Alleviating effects of dehydration under no hyperthermia on the immunomodulatory response to the polysaccharide fraction from fu-ling （Poria cocos） in male collegiate wrestlers

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Chinese Medical Journal 2011年第4期124卷 530-536页

作者： JANG Tsong-rong kao Ming-feng CHEN Chun-hao HSIEH Kuen-chang LAI Wen-yam CHEN Yu-yawn Athletics Department and Graduate School National Taiwan College of Physical EducationTaichung Taiwan China Department and Graduate School of Physical Education National Taiwan College of Physical EducationTaichung Taiwan China Department of Sport Tunghai University Taichung Taiwan China Research Center Charder Electronic Co. Ltd Taichung TaiwanChina Department and Graduate School of Physical Education National Taiwan College of Physical EducationTaichung Taiwan China

Background It is well known that dehydration can impair bodily functions. To evaluate the impact of hydration status under ambient environmental temperature on the immune system, 25 male collegiate wrestlers were recruited to undergo an experimental dehydration *** Thirteen subjects had controlled diets with individual energy requirements to prevent body mass loss and restricted water intake to cause 4.52% dehydration; they formed the dehydrated group （DE）. These subjects developed a urine specific gravity of about 1.030 in 84 hours. Twelve other subjects had no water restriction and maintained their total body weight comprised the euhydrated group （EU）. Peripheral blood monocytes （PBMNC） were isolated after dehydration to perform immune response testing by being incubated with a polysaccharide fraction from fu-ling, Poria cocos （polysaccharide fraction from Poria cocos, PCPS, 1-30 μg/L）, to prepare a conditioned medium termed conditioned medium of PBMNC stimulated by PCPS （PCPS-MNC-CM）. More PCPS （25 μg/L） was needed in the DE group to prepare the PCPS-MNC-CM, which was assayed with a growth inhibitory curve for treated U973 *** The treated U937 cells, incubated together with PCPS-MNC-CM from the DE group, exhibited a much lower nitroblue tetrazolium （NBT） positive value of （63.7±4.7）%. The concentration of interferon-gamma （IFN-Y）, interleukin （IL）-1β and tumor necrosis factor （TNF）-α in PCPS-MNC-CM from subjects after dehydration was much lower than in the CM from the EU *** The immune response to PCPS in the DE group was lower than in normally hydrated subjects.

关键词： dehydration mononuclear cells immunmodulatory U937 wrestlers

SuccSite:Incorporating Amino Acid Composition and Informative k-spaced Amino Acid Pairs to Identify Protein Succinylation Sites

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Genomics, Proteomics & Bioinformatics 2020年第2期18卷 208-219页

作者： Hui-Ju kao Van-Nui Nguyen Kai-Yao Huang Wen-Chi Chang Tzong-Yi Lee Department of Computer Science and Engineering Yuan Ze UniversityTaoyuan 32003TaiwanChina Department of Information Technology University of Information and Communication TechnologyThai Nguyen 1000Vietnam School of Science and Engineering The Chinese University of Hong KongShenzhen 518172China Warshel Institute for Computational Biology The Chinese University of Hong KongShenzhen 518172China Institute of Tropical Plant Sciences Cheng Kung UniversityTainan 701TaiwanChina

Protein succinylation is a biochemical reaction in which a succinyl group(-CO-CH2-CH2-CO-)is attached to the lysine residue of a protein *** succinylation plays important regulatory roles in living ***,studies in this field are limited by the difficulty in experimentally identifying the substrate site specificity of lysine *** facilitate this process,several tools have been proposed for the computational identification of succinylated lysine *** this study,we developed an approach to investigate the substrate specificity of lysine succinylated sites based on amino acid *** experimentally verified lysine succinylated sites collected from public resources,the significant differences in position-specific amino acid composition between succinylated and non-succinylated sites were represented using the Two Sample Logo *** findings enabled the adoption of an effective machine learning method,support vector machine,to train a predictive model with not only the amino acid composition,but also the composition of k-spaced amino acid *** the selection of the best model using a ten-fold crossvalidation approach,the selected model significantly outperformed existing tools based on an independent dataset manually extracted from published research ***,the selected model was used to develop a web-based tool,SuccSite,to aid the study of protein *** proteins were used as case studies on the website to demonstrate the effective prediction of succinylation *** will regularly update SuccSite by integrating more experimental *** is freely accessible at http://***/SuccSite/.

关键词： Protein succinylation Succinyl group Substrate specificity Amino acid composition k-spaced amino acid pair composition

Influence of graphene nanoplatelet incorporation and dispersion state on thermal, mechanical and electrical properties of biodegradable matrices

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Journal of Materials Science & Technology 2018年第6期34卷 1026-1034页

作者： Sima Kashi Rahul K.Gupta Nhol kao S.Ali Hadigheh Sati N.Bhattacharya Institute for Frontier Materials Deakin University Rheology and Materials Processing Group School of Engineering RMIT University School of Civil Engineering Faculty of Engineering and IT The University of Sydney

Graphene nanoplatelets （GNPs） were used as multifunctional nanofiller to enhance thermal and mechanical properties as well as electrical conductivity of two different biodegradable thermoplastics： poly lactide （PLA） and poly （butylene adipate-co-terephthalate）（PBAT）. Morphological investigations showed different levels of GNP dispersion in the two matrices, and consequently physical properties of the two systems exhibited dissimilar behaviours with GNP incorporation. Crystallinity of PLA, determined from differential scanning calorimetry, was observed to increase markedly with addition of GNPs in contrast to the decrease in crystallinity of PBAT. Isothermal and non-isothermal thermogravimetric analyses also revealed a more significant delay in thermal decomposition of PLA upon addition of GNPs compared to that of PBAT. Furthermore, results showed that increasing GNP content of PLA and PBAT nanocomposites influenced their Young＇s modulus and electrical conductivity in different ways. Modulus of PBAT increased continuously with increasing GNP loading while that of PLA reached a maximum at 9wt% GNPs and then decreased. Moreover, despite the higher conductivity of pure PBAT compared to pure PLA, conductivity of PLA/GNP nanocomposites overtook that of PBATIGNP nanocomposites above a certain GNP concentration. This demonstrated the determining effect of nanoplatelets dispersion state on the matrices properties.

关键词： Graphene Nanocomposite Poly lactide Poly butylene adipate-co-terephthalate Thermal stability Electrical conductivity Properties

In Situ/Operando(Soft) X-ray Spectroscopy Study of Beyond Lithium-ion Batteries

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Energy & Environmental Materials 2021年第2期4卷 139-157页

作者： Feipeng Yang Xuefei Feng Yi-Sheng Liu Li Cheng kao Per-Anders Glans Wanli Yang Jinghua Guo Joint Center for Energy Storage Research Lemont IL 60439USA Advanced Light Source Lawrence Berkeley National LaboratoryBerkeley CA 94720USA Department of Chemistry and Biochemistry University of California Santa CruzSanta Cruz CA 95064USA

The lightweight,rechargeable lithium-ion battery is one of the dominant energy storage devices globally in portable electronics due to its high energy density,no memory effect,wide operating voltage,lightweight,and good charge ***,due to safety concerns,the depletion of lithium reserves,and the corresponding increase of cost,an alternative battery system becomes more and more *** develop alternative battery systems with low cost and high material abundance,for example,sodium,magnesium,zinc,and calcium,it is important to understand the chemical and electronic structure of *** X-ray spectroscopy,for example,X-ray absorption spectroscopy(XAS),X-ray emission spectroscopy(XES),and resonant inelastic soft X-ray scattering(RIXS),is an element-specific technique with sensitivity to the local chemical environment and structural order of the element of *** soft X-ray systems enable operando experiments that can be applied to amorphous and crystalline samples,making it a powerful tool for studying the electronic and structural changes in electrode and electrolyte *** this article,the application of in situ/operando(soft)X-ray spectroscopy in beyond lithium-ion batteries is reviewed to demonstrate how such spectroscopic characterizations could facilitate the interpretation of interfacial phenomena under in situ/operando condition and subsequent development of the beyond lithium-ion batteries.

关键词： (soft)X-ray spectroscopy beyond lithium-ion battery in situ/operando interface