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Deciphering mycorrhizal fungi in cultivated Phalaenopsis microbiome with next-generation sequencing of multiple barcodes

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Fungal Diversity 2014年第3期66卷 77-88页

作者： Chao-Li huang Feng-Yin Jian hao-jen huang Wen-Chi Chang Wen-Luan Wu Chi-Chuan Hwang Ruey-Hua Lee Tzen-Yuh Chiang Department of Life Sciences National Cheng Kung UniversityTainanTaiwan 701 Institute of Tropical Plant Sciences National Cheng Kung UniversityTainanTaiwan 701 Department of Engineering Science and Supercomputing Research Center National Cheng Kung UniversityTainanTaiwan 701

Identifying the species composition of a microbial ecosystem is often hampered by difficulties in culturing the organisms and in the low sequencing depth of traditional DNA *** analysis,a huge-scale nucleotide-sequence-based tool,can overcome such *** this study,Sanger sequencing of 500 nrITS clones uncovered 29 taxa of 19 fungal genera,whereas metagenomics with next-generation sequencing identified 512 operational taxonomic units(OTUs)for ITS1/2 and 364 for ITS3/***,high throughput sequencing of PCR amplicons of ITS1/2,ITS3/4,nrLSU-LR,nrLSU-U,mtLSU,and mtATP6,all with at least 1,300×coverage and about 21 million reads in total,yielded a very diverse fungal *** fact that 74%of the OTUs were exclusively uncovered with single barcodes indicated that each marker provided its own insights into the fungal *** deal with the high heterogeneity in the data and to integrate the information on species composition across barcodes,a rank-scoring strategy was ***,205 genera among 64 orders of fungi were identified in healthy Phalaenopsis *** the barcodes utilized,ITS1/2,ITS3/4,and nrLSU-U were the most competent in uncovering the fungal *** barcodes,though detecting different compositions likely due to primer preference,provided complementary and comprehensive power in deciphering the microbial diversity,especially in revealing rare species.

关键词： DNA barcode Metagenomics Mycorrhizal fungi Next-generation sequencing Phalaenopsis orchid Rank scoring

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Sutured scleral fixation of existing subluxated/dislocated acrylic one-piece intraocular lenses

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 665-669页

作者： Wei-Yu huang Yung-jen Chen Department of Ophthalmology Chi Mei Medical CenterTainan City 710TaiwanChina Department of Ophthalmology Chang Gung Memorial HospitalKaohsiung City 833TaiwanChina

AIM:To evaluate the visual and refractive outcomes in cases after sutured scleral fixation of existing subluxated or dislocated acrylic one-piece intraocular lenses(IOLs).METHODS:This study retrospectively enrolled a consecutive series of patients who underwent a surgery of sutured existing subluxated or dislocated IOLs from October 2018 to June *** patients underwent comprehensive preoperative and postoperative ophthalmologic examination,and data were collected including age,sex,surgical indications,best-corrected visual acuity,refractive error,intraocular *** of intraoperative and postoperative surgical complications was ***:A total of 20 consecutive cases were enrolled for analysis with mean final follow-up period 9.8±*** acuity improved from a mean of 0.35(0.46±0.32 logMAR)preoperatively to 0.61(0.21±0.18 logMAR)at the 3-month follow-up(P=0.002).The mean amount of preoperative keratometric astigmatism and total postoperative refractive astigmatism was-1.24±0.80 diopters(D)and-1.42±0.97 D,*** was no statistically significant difference between preoperative and postoperative astigmatism(P=0.156).The mean IOL-induced astigmatism was-0.23±0.53 *** mean spherical equivalent at the 3-month follow-up was-0.1±0.94 *** major complications were noted during the follow-up ***:Surgical techniques using sutured scleral fixation of existing subluxated or dislocated acrylic one-piece IOLs result in favorable visual and refractive outcomes without major complications.

关键词： intraocular lens dislocation intraocular lens subluxation lens dislocation lens subluxation scleral fixation

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Laminar Flame Structure-Dependent Exergy Destruction Behavior at Auto-Ignition Time Scale:A Case Study of Dimethyl Ether(DME)

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Journal of Thermal Science 2024年第4期33卷 1257-1271页

作者： WU Honghuan huang Wenlin Zhao hao SUN Wuchuan huang Zuohua ZHANG Yingjia State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong UniversityXi’an710049China

Hybrid deflagration/auto-ignition flame structures coexist in the combustion of advanced *** exergy destruction caused by different irreversible processes under varied flame regimes is thus important for understanding engine *** this study,the flame propagation modes for the premixed DME/air mixtures are numerically investigated under engine-relevant *** entropy generation and exergy destruction characteristics are compared under different flame *** reveal that as the typical premixed flame transition towards auto-ignition front,the exergy destruction from heat conduction and species mass diffusion gradually vanish and are dominated by chemical *** distributions of temperature and species mole fraction in the flame domain are analyzed to clarify the exergy destruction behaviors caused by heat conduction and mass ***,by dividing the DME oxidation process into four stages,the main reaction channels that contribute to the increase in exergy destruction from chemical reaction have been *** is found that the production and consumption of CH_(2)O and HO_(2) radical dominate the exergy destruction behavior during DME *** this basis,the kinetic mechanism of low-temperature chemistry causing greater exergy destruction is ***,low-temperature chemistry leads to significant exergy destruction due to(a)the large irreversibility of itself and(b)(mainly)the enhancement of H_(2)O_(2)loop reactions by low-temperature reaction *** the reduction of combustion irreversibility is promising to be achieved by reasonably regulating the fuel oxidation path.

关键词： exergy destruction flame structures entropy generation low-temperature chemistry DME

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Geochronology and Geochemistry of the Xingxingxia Triassic A-type Granites in Central Tianshan,NW China:Petrogenesis and Tectonic Implications

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Acta Geologica Sinica(English Edition) 2024年第2期98卷 337-351页

作者： huang Zengbao LI Xiyao Zhao hao LU Qing Geological Survey of Gansu Province Lanzhou 735000China Key Laboratory of Submarine Geosciences and Prospecting Techniques MOE and College of Marine GeosciencesOcean University of ChinaQingdaoShandong 266100China Research Institute of Petroleum Exploration and Development Shengli OilfieldSINOPECDongyingShandong 257000China

The Triassic granitoids in Central Tianshan play a key role in determining the petrogenesis and tectonic evolution on the southern margin of the Central Asian orogenic *** this study,we present SHRIMP zircon U-Pb ages,Hf isotopic and geochemical data on the Xingxingxia biotite granite,amazonite granite and granitic pegmatite in Central Tianshan,NW *** U-Pb dating yielded formation ages of 242 Ma for the biotite granite and 240 Ma for the amazonite *** granitoid rocks have high K_(2)O with low MgO and CaO *** are enriched in Nb,Ta,Hf and Y,while being depleted in Ba and Sr,showing flat HREE patterns and negative Eu *** have typical A-type granite geochemical signatures with high Ga/A_(1)(8–13)and TFeO/(TFeO+MgO)ratios,showing an A_(2) affinity for biotite granite and an A_(1) affinity for amazonite granite and granitic *** ε_(Hf)(t)values of the granitoids are 0.45–2.66,with Hf model ages of 0.99–1.17 *** suggests that these A-type granites originated from partial melting of the lower *** propose that Xingxingxia Triassic A-type granites formed under lithospheric extension from post-orogenic to anorogenic intraplate settings and NE-trending regional strike-slip fault-controlled magma emplacement in the upper crust.

关键词： Triassic A-type granite Xingxingxia Central Tianshan Central Asian orogenic belt

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Fractal Characteristics and Main Controlling Factors of High-Quality Tight Sandstone Reservoirs in the Southeastern Ordos Basin

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Journal of Earth Science 2024年第2期35卷 631-641页

作者： Ruilin hao Wenhui huang Jiu Bo Li Yuan Key Laboratory for Marine Reservoir Evolution and Hydrocarbon Abundance Mechanism School of Energy ResourcesChina University of GeosciencesMinistry of EducationBeijing100083China

Due to the complex conditions and strong heterogeneity of tight sandstone reservoirs,the reservoirs should be classified and the controlling factors of physical properties should be *** thin section observations,cathodoluminescence,scanning electron microscopy(SEM),X-ray diffraction(XRD),and high-pressure mercury injection(HPMI)were used to classify and optimize the *** Brooks-Corey model and stepwise regression were used to study the fractal dimension and main controlling factors of the physical properties of the high-quality *** results show that the reservoirs in the study area can be divided into four types,and the high-quality reservoir has the best physical properties and pore-throat *** the high-quality reservoir,the homogeneity of transitional pores was the best,followed by that of micropores,and the worst was *** porosity was controlled by depth and *** model with standardized coefficients is y=12.454−0.778×(Depth)+0.395×(Kaolinite).The permeability was controlled by depth,illite/montmorillonite,and siliceous cement,and the model with standardized coefficients is y=1.689−0.683×(Depth)−0.395×(Illite/Montmorillonite)−0.337×(Siliceous Cement).The pore-throat evolutionary model shows that the early-middle diagenetic period was when the reservoir physical properties were at their best,and the kaolinite intercrystalline pores and residual intergranular pores were the most important.

关键词： fractal dimension main controlling factors stepwise regression high-quality tight sandstone reservoir Ordos Basin petroleum geolgoy

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Lead-free perovskites-based photonic synaptic devices with zero electric energy consumption

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Science China(Information Sciences) 2024年第6期67卷 433-441页

作者： Dandan hao Di YANG Haixia LIANG Jia huang Fukai SHAN College of Electronics and Information Qingdao University Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences School of Materials Science and Engineering Tongji University National Key Laboratory of Autonomous Intelligent Unmanned Systems Tongji University

The von Neumann bottleneck is a critical limitation in synaptic devices. Therefore, artificial synaptic devices resembling biological neuromorphic synapses have been developed to overcome the von Neumann bottleneck. However, synaptic devices require voltages, which results in considerable energy consumption. Here, photonic synaptic devices with the vertical structure of indium tin oxide（ITO）/SnO2/Al2O3/CsBi3I10/Au are fabricated, which can work in the self-powered mode owing to the photovoltaic effect endowed by a vertical multilayer structure. Several fundamental synaptic functions, such as excitatory postsynaptic current, paired-pulse facilitation, short-term plasticity（STP）, long-term plasticity（LTP）, pulse-frequency-dependent plasticity, transition of STP to LTP, and the learning experience are emulated. Moreover, Morse-coded external light information is decoded by self-powered photonic synaptic devices. The results indicate that self-powered photonic synaptic devices based on lead-free perovskites exhibit great potential for efficient neuromorphic computing and optical wireless communication.

关键词： photonic synaptic devices lead-free perovskites zero energy consumption synaptic plasticity Morse code

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Radical involved reactive wetting and retarding mechanism of alumina refractory ceramic by molten slags under weak static magnetic field

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Journal of Iron and Steel Research International 2024年第6期31卷 1355-1366页

作者： Sheng-hao Li Ao huang Fan-bo Zeng Hui Peng hao-ran Wei Xue-chun huang Sheng-qiang Song Hua-zhi Gu The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina

High alumina slag will cause severe corrosion at the interface of alumina refractory,and the wetting behavior of slag is a key factor influencing the corrosion resistance of refractory *** static magnetic field is a promising solution for improvement in the slag resistance of *** wetting of alumina refractory ceramics with different basicities of high alumina slags under a weak static magnetic field was analyzed,given that a weak static magnetic field can affect the corrosion behavior of refractory *** slag S_(3) as an example,when there was an external static magnetic field of 1.0 mT at 1600 ℃,the thickness of calcium aluminate reaction layer at the interface decreased by 36.7%,the denting depth of interface decreased by 35.6%,and the apparent wetting angle increased by 20%.The living radicals and their formation path in oxide melts were verified by first-principles calculation combined with electron paramagnetic resonance spectroscopy *** influence of the flux density of a weak static magnetic field on the wetting behavior of slags was also *** contact angle of the slags increased owing to the inhibitory effect of magnetic field on the radicalinvolved reaction at the interface of the slag and the alumina refractory *** relationships between the magnetic flux density,diffusion coefficient,slag microstructure(hyperfine coupling constant),and contact angle were *** provides a theoretical basis for the field control of radical involved reactive wetting between inorganic oxide slags and solid oxide ceramics.

关键词： Alumina refractory ceramic Molten slag Weak magnetic field effect Reactive wetting Free radical

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Bibliometric analysis and systematic review of electrochemical methods for environmental remediation

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Journal of Environmental Sciences 2024年第10期144卷 113-136页

作者： Wenbin huang Shibin Liu Tao Zhang hao Wu Shengyan Pu College of Earth Sciences Chengdu University of TechnologyChengdu 610059China State Key Laboratory of Geohazard Prevention and Geoenvironment Protection(Chengdu University of Technology) Chengdu 610059China College of Ecology and Environment Chengdu University of TechnologyChengdu 610059China Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Tibet Plateau in Qinghai Province Qinghai Normal UniversityXining 810008China Scientific Research Academy of Guangxi Environmental Protection Nanning 530022China

Electrochemical methods are increasingly favored for remediating polluted environments due to their environmental compatibility and reagent-saving ***,a comprehensive understanding of recent progress,mechanisms,and trends in thesemethods is currently *** of Science(WoS)databases were utilized for searching the primary data to understand the knowledge structure and research trends of publications on electrochemical methods and to unveil certain hotspots and future trends of electrochemical methods *** original data were sampled from 9080 publications in those databases with the search deadline of June 1st,*** and VOSviewer software facilitated data visualization and analysis of document quantities,source journals,institutions,authors,and *** discussed principles,influencing factors,and progress related to seven major ***,publications on this subject have experienced significant growth since *** most frequently-investigated areas in electrochemical methods included novel materials development,heavy metal remediation,organic pollutant degradation,and removal mechanism identification.“Advanced oxidation process”and“Nanocomposite”are currently trending *** major remediation mechanisms are adsorption,oxidation,and *** efficiency of electrochemical systems is influenced by material properties,system configuration,electron transfer efficiency,and power ***-Fenton exhibits significant advantages in achieving synergistic effects of anodic oxidation and electro-adsorption among the seven *** research should prioritize the improvement of electron transfer efficiency,the optimization of electrode materials,the exploration of emerging technology coupling,and the reduction in system operation and maintenance costs.

关键词： Electrochemical remediation Bibliometric analysis Heavy metals Organic pollutants Electrode materials

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A LiDAR Point Clouds Dataset of Ships in a Maritime Environment

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IEEE/CAA Journal of Automatica Sinica 2024年第7期11卷 1681-1694页

作者： Qiuyu Zhang Lipeng Wang hao Meng Wen Zhang Genghua huang the College of Intelligent Systems Science and Engineering Harbin Engineering UniversityHarbin 150001China Key Laboratory of Space Active Opto-Electronics Technology Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China

For the first time, this article introduces a LiDAR Point Clouds Dataset of Ships composed of both collected and simulated data to address the scarcity of LiDAR data in maritime applications. The collected data are acquired using specialized maritime LiDAR sensors in both inland waterways and wide-open ocean environments. The simulated data is generated by placing a ship in the LiDAR coordinate system and scanning it with a redeveloped Blensor that emulates the operation of a LiDAR sensor equipped with various laser beams. Furthermore,we also render point clouds for foggy and rainy weather conditions. To describe a realistic shipping environment, a dynamic tail wave is modeled by iterating the wave elevation of each point in a time series. Finally, networks serving small objects are migrated to ship applications by feeding our dataset. The positive effect of simulated data is described in object detection experiments, and the negative impact of tail waves as noise is verified in single-object tracking experiments. The Dataset is available at https://***/zqy411470859/ship_dataset.

关键词： 3D point clouds dataset dynamic tail wave fog simulation rainy simulation simulated data

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Interaction Mechanisms between Natural Debris Flow and Rigid Barrier Deflectors:A New Perspective for Rational Design and Optimal Arrangement

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 1679-1699页

作者： Yu huang Beilei Liu Dianlei Feng hao Shi Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai200092China Department of Hydraulic Engineering College of Civil EngineeringTongji UniversityShanghai200092China

Rigid barrier deflectors can effectively prevent overspilling landslides,and can satisfy disaster prevention ***,the mechanisms of interaction between natural granular flow and rigid barrier deflectors require further *** date,few studies have investigated the impact of deflectors on controlling viscous debris flows for geological disaster *** investigate the effect of rigid barrier deflectors on impact mechanisms,a numerical model using the smoothed particle hydrodynamics(SPH)method with the Herschel–Bulkley model is proposed to simulate the interaction between natural viscous flow and single/dual barriers with and without *** model was validated using laboratory flume test data from the ***,the model was used to investigate the influence of the deflector angle and multi-barrier *** optimal configuration of multi-barriers was analyzed with consideration to the barrier height and distance between the barriers,because these metrics have a significant impact on the viscous flow pile-up,run-up,and overflow *** investigation considered the energy dissipation process,retention efficiency,and dead-zone *** with bare barriers with similar geometric characteristics and spatial distribution,rigid barriers with deflectors exhibit superior effectiveness in preventing the overflow and overspilling of viscous debris *** for the rational design of deflectors and the optimal arrangement of multi-barriers are provided to mitigate geological disasters.

关键词： Rigid barrier deflectors deflector angle single-barrier dual-barrier arrangements viscous debris flow over-spilling delta-plus-SPH

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