检索结果-南通市图书馆

Synthesis and photo activity of phenylazonaphthalene peptide nucleic acid monomers

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2008年第7期19卷 807-810页

作者： Jin Du Li Miao Chen Sheng Liu hao bo zhang Zhi Lu Liu Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 PR China Graduate School of Chinese Academy of Sciences Beijing 100039 PR China

Phenylazonaphthalene peptide nucleic acid （PNA） monomers were successfully synthesized, and their photoisomerization was examined. The new PNA monomers showed reversible trans-cis isomerization with UVand visible light irradiation, which might be the foundation of photo-regulating the hybridization between PNA containing phenylazonaphthalene unit and DNA. Simultaneously, the fluorescence of the new PNA monomers might make them especially useful as structural probes.

关键词： Peptide nucleic acid Phenylazonaphthalene Photoisomerization UV-vis absorption spectrometry Fluorescence spectrometry

Decline of semen quality among Chinese sperm bank donors within 7 years （2008-2014）

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第5期19卷 521-525页

作者： Li Wang Lin zhang Xiao-Hui Song hao-bo zhang Cheng-Yan Xu Zi-Jiang Chen Center for Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University Jinan China Shandong Provincial Key Laboratory of ReproductiveMedicine Jinan China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan China Key Laboratory for ReproductiveEndocrinology of Ministry of Education Jinan China

Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of semen volume, sperm concentration, sperm forward motility, and total sperm count were performed. There were significant declining trends in semen volume, sperm concentration, sperm forward motility, and total sperm count. Our results indicate that the quality of semen in this cohort of sperm donors had decreased during the study period.

关键词： male infertility semen quality sperm donation

Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2008年第2期10卷 227-235页

作者： hao-bo zhang Shao-Ming Lu Chun-Yan Ma Li Wang Xiao Li Zi-Jiang Chen Center for Reproductive Medicine Affiliated to Shandong University Jinan 250021 China Central Labotrary of Provincial Hospital Affiliated to Shandong University Jinan 250021 China

Aim： To investigate whether early apoptotic changes in spermatozoa can be significant markers for sperm quality. Methods： Two early apoptotic changes in the semen of 56 men were assessed using Annexin V （AN）/propidium iodide （PI） staining for phosphatidylserine externalization and JC-1 staining for mitochondrial membrane potential （MMP）. The results were compared with conventional semen parameters and DNA fragmentation identified using the TUNEL assay. Results： The different labeling patterns in the bivariate Annexin V/PI analysis identified four distinctive spermatozoa populations. The percentage of AN^-/PI^- spermatozoa positively correlated with conventional semen parameters and MMP, but negatively correlated with TUNEL （＋） spermatozoa. As for the AN^-/PI^＋ fraction, we found an opposite result in comparison to AN^-/PI^- spermatozoa. The level of early apoptotic AN^＋/PI^- spermatozoa negatively correlated with MMP and sperm motility. The level of late apoptotic AN^＋/PI^＋ spermatozoa negatively correlated with conventional semen parameters and MMP, and positively correlated with TUNEL （＋） spermatozoa. MMP positively correlated with conventional semen parameters, but negatively correlated with TUNEL （＋） spermatozoa. Conclusion： Although early apoptotic AN^＋/PI^- spermatozoa only negatively correlates with sperm motility, the differences in proportion of each subpopulation of spermatozoa （especially, the percentage of AN^-/PI^- spermatozoa）, and decreased MMP might be significant markers for diagnosing male infertility. They possibly bring additional information to predict the outcome of in vitro fertilization. （Asian J Androl 2008 Mar; 10： 227-235）

关键词： Annexin V apoptosis DNA fragmentation infertility mitochondria sperm

Ileal pouch anal anastomosis with modified double-stapled mucosectomy-the experience in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第8期19卷 1299-1305页

作者： Ya-Jie zhang Yi Han Mou-Bin Lin Yong-Gang He hao-bo zhang Lu Yin Liang Huang Department of General Surgery RuiJin Hospital Affiliated Shanghai Jiaotong University School of Medicine Department of Emergency and Trauma Surgery RuiJin Hospital Affiliated Shanghai Jiaotong University School of Medicine

AIM:To investigate the feasibility and long-term functional outcome of ileal pouch-anal anastomosis with modified double-stapled ***:From January 2002 to March 2011,fourtyfive patients underwent ileal pouch anal anastomosis with modified double-stapled mucosectomy technique and the clinical data obtained for these patients were ***:Patients with ulcerative colitis(n = 29) and familial adenomatous polyposis(n = 16) underwent ileal pouch-anal anastomosis with modified doublestapled ***-eight patients underwent one-stage restorative proctocolectomy,ileal pouch anal anastomosis,protective ileostomy and the ileostomy was closed 4-12 mo ***-stage procedures were performed in seventeen urgent patients,proctectomy and ileal pouch anal anastomosis were completed after previous colectomy with *** within the first 30 d of surgery occurred in 10(22.2%) patients,all of them could be treated *** the median follow-up of 65 mo,mild to moderate anastomotic narrowing was occurred in 4 patients,one patient developed persistent anastomotic stricture and need surgical *** percent of patients developed at least 1 episode of *** was no incontinence in our patients,the median functional Oresland score was 6,3 and 2 after 1 year,2.5 years and 5 years *** half patients(44.4%) reported "moderate functioning",37.7% reported "good functioning",whereas in 17.7% of patients "poor functioning" was observed after 1 *** years later,79.2% of patients with good function,16.7% with moderate function,only 4.2% of patients with poor ***:The results of ileal pouch anal anastomosis with modified double-stapled mucosectomy technique are promising,with a low complication rate and good long-term functional results.

关键词： Ileal pouch anal anastomosis Stapled mucosectomy Ulcerative colitis Familial adenomatous polyposis Surgical technique

A new TEX11 mutation causes azoospermia and testicular meiotic arrest

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2021年第5期23卷 510-515页

作者： Xiao-Chen Yu Meng-Jing Li Fei-Fei Cai Si-Jie Yang Hong-Bin Liu hao-bo zhang Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Jinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 25CX312China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China

There are many unknown genetic factors that lead to infertility in nonobstructive azoospermia ***,we performed whole-exome sequencing in blood samples obtained from 40 azoospermia patients with meiotic arrest and found a novel c.151_154del(p.D51fs)frame-shift mutation in exon 3 of the testis expressed 11(TEX11)gene in one *** sequencing analysis of the patient and 288 fertile men was performed to validate the *** analysis showed TEX11 expression in late-pachytene spermatocytes and in round spermatids in fertile human *** contrast,testes of the patient with TEX11 mutation underwent meiotic arrest and lacked TEX11 *** blotting of human embryonic kidney(HEK293)cells transfected with a vector for the p.D51fs TEX11 variant detected no TEX11 *** conclusion,we identified a novel frame-shift mutation in the TEX11 gene in an azoospermia patient,emphasizing that this gene should be included in genetic screening panels for the clinical evaluation of azoospermia patients.

关键词： male infertility meiotic arrest nonobstructive azoospermia testis expressed 11

Mutational analysis of the GATA4 gene in Chinese men with nonobstructive azoospermia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2021年第2期23卷 205-210页

作者： Xu zhang Tai-Jian zhang Wen Liu Yun-Na Ning Yue-Hong Bian Yong-Zhi Cao Hong-Bin Liu Jin-Long Ma hao-bo zhang Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China National Research Centre for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology School of Life SciencesShandong UniversityQingdao 266237China

As a crucial transcription factor for spermatogenesis,GATA-binding protein 4(GATA4)plays important roles in the functioning of Sertoli and Leydig *** knockout of GATA4 in mice results in age-dependent testicular atrophy and loss of ***,whether GATA4 is associated with human azoospermia has not been ***,we analyzed the GATA4 gene by direct sequencing of samples obtained from 184 Chinese men with idiopathic nonobstructive azoospermia(NOA).We identified a missense mutation(c.191G>A,p.G64E),nine single-nucleotide polymorphisms(SNPs),and one rare variant(c.^(*)84C>T)in the 3′untranslated region(UTR).Functional studies demonstrated that the p.G64E mutation did not affect transactivation ability of GATA4 for spermatogenesis-related genes(claudin-11 and steroidogenic acute regulatory protein,Star),and the 3′UTR rare variant c.^(*)84C>T did not generate microRNA-binding sites to repress GATA4 *** our knowledge,this is the first report to investigate the association between GATA4 and azoospermia;our results indicate that mutations in GATA4 may not be pathogenic for NOA in Chinese men.

关键词： biological function GATA-binding protein 4(GATA4)mutation nonobstructive azoospermia

Predictive significance of serum inhibin B on testicular haploid gamete retrieval outcomes in nonobstructive azoospermic men

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2019年第2期21卷 137-142页

作者： Zhi-Guo Zhu Zhi-Gang Zhao Qing-Yang Pang Tong Chen Jian-Min zhang Tai-Jian zhang Chao Xu hao-bo zhang Wen Liu Xu-Jun Xuan Department of Urology and Andrology Minimally Invasive Surgery CenterThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510230China Guangzhou Institute of Urology Guangzhou 510230China Guangdong Key Laboratory of Urology Guangzhou 510230China Center for Reproductive Medicine Shandong UniversityJinan 250001China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan 250001China The Key Laboratory for Reproductive Endocrinology of Ministry of Education Jinan 250001China Shandong Xiehe University Jinan 250109China

The purpose of this study was to determine the diagnostic accuracy of serum inhibin B(INHB)as a predictor of the retrieval outcome of testicular haploid gametes(spermatids and testicular spermatozoa)in nonobstructive azoospermic *** hormone levels,testicular volume,and histological evaluation were performed in 403 Chin ese non obstructive azoospermic *** haploid gamete was successfully retrieved in 213 of 403 patients(52.85%).The haploid gamete group always had higher INHB levels than the non-haploid gamete *** to the receiver operating characteristic(ROC)curve analysis,INHB was a good predictor of testicular haploid gamete retrieval outcome in all patients(sensitivity:77.93%and specificity:91.58%)and patients with normal follicle-stimulating hormone(FSH;sensitivity:88.52%and specificity:70.83%).The area under the ROC curve(AUC)of INHB was similar to that of FSH in all patients or patients with normal *** patients with elevated FSH,INHB was superior to FSH in predicting the presence of haploid gamete(AUC:0.73 vs 0.55,P<0.05),with a sensitivity of 60.00%and a specificity of 80.28%.It con eluded that serum INHB as an effective marker for spermatoge nesis was a sign ificant predictor of testicular haploid gamete retrieval outcomes in nonobstructive azoospermic ***,INHB is superior to FSH in predicting the presenee of haploid gamete in the patients with elevated FSH.

关键词： azoospermia biomarker follicle-stimulating hormone inhibin B testicular sperm extraction

Assessment on Chloride Contaminated Resistance of Concrete with Non-steady-state Migration Method

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2004年第4期19卷 6-8页

作者：候浩波 Wuhan University

A preparation technology of chloride contaminated resistant concrete was presented. The newly developed non-steady-state migration test method was employed for the evaluation of chloride ions diffusion coefficient of the concrete. The experimental results show that this method has a good correlation with the traditional NT Build 443 method, and it provides an effective and quick approach to assess the resistant capacity of concrete against chloride ions.

关键词： high performance concrete durability chloride contaminated diffusion coefficient

Outcomes of Preimplantation Genetic Diagnosis Cycles by Fluorescent In situ Hybridization of Infertile Males with Nonmosaic 47,XYY Syndrome

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2018年第15期131卷 1808-1812页

作者： Chao Xu Fang-Fang zhang Hong-Chang Li Miao-Miao Wang Yue-Ting Zhu Wen-Jie Jiang Yue Wang hao-bo zhang Rong Tang Gang Ma Jun-hao Yah Center for Reproductive Medicine Shandong University Jinan Shandong 250001 China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan Shandong 250001 China The Key Laboratory for Reproductive Endocrinology of Ministry of Education Jinan Shandong 250001 China Department of Urology Qilu Hospital of Shandong University Jinan Shandong 250012 China Center for Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong 250001 China

Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization （FISH） and preimplantation genetic diagnosis （PGD） treatment. Methods： This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study. According to sex chromosomal FISH results, embryos were classified as normal signal, no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups, respectively. The incidence of each group, the fixation rate, and hybridization rate were calculated. Embryonic development and pregnancy outcomes were also analyzed. The measurement data were analyzed with Student＇s t-test. The comparison of categorical data was analyzed with the Chi-square test and Fisher＇s exact test when expected cell count was 〈5. Results： The 53 PGD cycles with 433 embryos were analyzed. The fixation rate was 89.6%, while the hybridization rate was 96.4%. There were 283 embryos with two sex chromosomal signals with clear diagnosis （65.4%）. The numbers of no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups were 45 （10.4%）, 14 （3.2%）, 24 （5.5%）, and 67 （15.5%）, respectively. Embryos with abnormal signals were abandoned. The number of good-quality embryos was 210 （57.4%）, including implanted embryos on day 4/day 5 *** cryopreserved. The rates of good-quality embryos in the no nuclei fixed （22.2%）, no signal in fixed nuclei （28.6%）, and suspensive signal groups （33.3%） were comparable （P 〉 0.05）, and were significantly lower than the normal signal group （66.4%, P 〈 0.001 ）. The clinical pregnancy rates of fresh and frozen embryos transferred cycles were 70.6% and 85.7%, respectively. Co

关键词： 47,XYY Syndrome Fluorescent In situ Hybridization Infertility Pregnancy Outcome Preimplantation GeneticDiagnosis