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High glucose causes apoptosis of rabbit corneal epithelial cells involving activation of PERK-eIF2α-CHOP-caspase-12 signaling pathway

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International Journal of Ophthalmology(English edition) 2019年第12期12卷 1815-1822页

作者： Pan-Pan Yao Min-Jie Sheng Wen-hao weng Yin Long hao Liu Li Chen Jia-Jun Lu Ao Rong Bing Li Department of Ophthalmology Yangpu HospitalTongji University School of MedicineShanghai 200090China Department of Clinical Laboratory Yangpu HospitalTongji University School of MedicineShanghai 200090China Department of Ophthalmology Tongji HospitalTongji University School of MedicineShanghai 200065China

AIM: To investigate the effect of high concentration of glucose(HCG) on double stranded RNA-activated protein kinase-like ER kinase(PERK)-eukaryotic initiation factor-2α(eIF2α)-transcription factor C/EBP homologous protein(CHOP)-cysteine aspartate specific proteinase(caspase-12) signaling pathway activation and apoptosis in rabbit corneal epithelial cells(RCECs). METHODS: RCECs were treated by different concentrations of glucose for 0-48 h. The expressions of PERK, p-PERK, eIF2α, p-eIF2α, 78 k Da glucose-regulated protein 78(GRP78), CHOP, B-cell lymphoma 2(Bcl-2), B-cell lymphoma-2-associated X protein(Bax) and caspase-12 were determined by Western blot. Apoptosis was detected by TUNEL assay. Meanwhile, the function of PERK-eI F2α-CHOP-caspase-12 signaling pathway activation in high glucose-induced apoptosis was evaluated using PERK inhibitor, GSK2606414. RESULTS: HCG significantly promoted the expression of p-PERK, p-eIF2α, GRP78, CHOP, Bax and cleaved caspase-12 in RCECs(P0.05). TUNEL assay showed that the apoptosis rate of RCECs in the HCG group increased significantly in contrast with that in the normal concentration of glucose or osmotic pressure control group(P0.05). Correspondingly, GSK2606414 also significantly reduced the apoptosis rate induced by high glucose(P<0.05). CONCLUSION: HCG activates PERK-eIF2α-CHOPcaspase-12 signaling pathway and promotes apoptosis of RCECs.

关键词： high glucose rabbit corneal epithelial cells PERK-eIF2α-CHOP-caspase-12 pathway apoptosis

Pseudo-Wellens syndrome secondary to postexercise syncope

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World Journal of Emergency Medicine 2022年第4期13卷 334-336页

作者： Shu Fang Zhi-hao Liu Qi Jin Yu-xi Li Wen-hui Ding hao-yu weng Tie-ci Yi Jian-ping Li Department of Cardiology Peking University First HospitalBeijing 100034China Department of Cardiology Xiamen Cardiovascular HospitalXiamen UniversityXiamen 361004China Department of Cardiology Shanghai Institute of Cardiovascular DiseasesZhongshan HospitalFudan UniversityShanghai 200032China Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education Health Science CenterPeking UniversityBeijing 100034China

Dear editor,Wellens syndrome is characterized by electrocardiography(ECG)patterns of the biphasic or inverted T wave in the precordial leads with normal R wave progression and absence of Q waves,[1,2]which indicates occlusive lesions of the proximal left anterior descending(LAD)coronary artery and warrants.[1]These ECG changes usually occur during a pain-free interval and turn to a fatal consequence if without urgent intervention therapy.[1,3]However,in some cases,coronary stenosis was absent and ECG patterns manifested as Wellens syndrome,which was defined as pseudo-Wellens syndrome.

关键词： coronary editor consequence

Modeling pollutant transport in overland flow over non-planar and non-homogenous infiltrating surfaces

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第2期14卷 110-119页

作者： Zhi-guo HE Gokmen TAYFUR Qi-hua RAN hao-xuan weng Department of Ocean Science and Engineering Zhejiang University Department of Civil Engineering Izmir Institute of TechnologyGulbahce KampusUrlaIzmir 35340Turkey Department of Hydraulic Engineering Zhejiang University

Pollutant transport in overland flow over surfaces with spatially varying microtopography,roughness,and infiltration was investigated using the diffusion wave equation and transport rate-based *** finite volume method in space and an implicit backward difference scheme in time were employed in the numerical solution of the 2D governing *** developed model was first tested against an analytical solution and an experimental study involving overland flow and the associated pollutant transport,subsequently a series of numerical tests were carried ***-point source pollution was investigated under spatially varying microtopography,roughness,and *** simulation results showed that microtopography and roughness were the dominant factors causing significant spatial variations in solute *** the spatially varying microtopography was replaced by a smooth surface,the result was an overestimation of the solute rate at the outlet of the *** the other hand,when the spatially varying roughness was replaced by the average roughness and spatially varying infiltration rate by the average infiltration rate,the pollutant discharge at the outlet of the upland was not significantly *** numerical results further showed that one cannot ignore the spatial variations of slope and roughness when investigating the local pollutant concentration distribution.

关键词： Diffusion wave Variation Topography Roughness Infiltration Pollutant Modeling Overland flow

Association between composite dietary antioxidant index and stroke among individuals with diabetes

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World Journal of Diabetes 2024年第8期15卷 1742-1752页

作者： Hong-Qiang Zhang Jie Shi Tong Yue Jia-hao weng Xu-Lin Wang hao Wang Xiao-Yu Su Xue-Ying Zheng Si-Hui Luo Yu Ding Chao-Fan Wang Department of Cardiology Centre for Leading Medicine and Advanced Technologies of IHMThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Department of Endocrinology Centre for Leading Medicine and Advanced Technologies of IHMThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Graduate School Bengbu Medical CollegeBengbu 233030Anhui ProvinceChina Department of Endocrinology and Metabolism The Third Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of DiabetologyGuangzhou 510630Guangdong ProvinceChina

BACKGROUND Recent research has underscored the potentially protective role of dietary antioxidants against chronic conditions,such as cardiovascular diseases and *** composite dietary antioxidant index(CDAI),which reflects the overall intake of key dietary antioxidants,has been identified as a crucial metric for exploring this *** previous research has shown a negative correlation between CDAI levels and stroke risk in prediabetic individuals,there remains a substantial gap in understanding this association among individuals with diabetes,who are at an inherently greater risk for cerebrovascular *** To investigate the association between CDAI and stroke risk in individuals with *** Using a cross-sectional study design,this investigation analyzed data from the National Health and Nutrition Examination Survey spanning from 2003 to 2018 that included 6735 participants aged over 20 years with *** CDAI was calculated from 24-h dietary recalls to assess intake of key antioxidants:Vitamins A,C,and E;carotenoids;selenium;and *** logistic regression and restricted cubic spline analysis were used to rigorously examine the relationship between CDAI and stroke *** The participant cohort,with an average age of 59.5 years and a slight male majority,reflected the broader demographic characteristics of individuals with *** analysis revealed a strong inverse relationship between CDAI levels and stroke ***,those in the highest quintile of CDAI demonstrated a 43%lower prevalence of stroke compared to those in the lowest quintile,even after adjustments for various *** finding not only highlights the negative association between CDAI and stroke risk but also underscores the significant potential of antioxidant-rich diets in reducing stroke prevalence among patients with *** Our findings suggested that CDAI was inversely associated with stroke prevalence among pa

关键词： Stroke Diabetes Composite dietary antioxidant index National Health and Nutrition Examination Survey Crosssectional study

The role of prophylactic transpapillary pancreatic stenting in distal pancreatectomy;a meta-analysis

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Frontiers of Medicine 2013年第4期7卷 499-505页

作者： Xiangsong Wu Maolan Li wenguang Wu Jiasheng Mu Lin Zhang Qichen Ding Qian Ding hao weng Runfa Bao Yijun Shu Ping Dong Jun Gu Jianhua Lu Yingbin Liu Department of General Surgery Xinhua HospitalShanghai JiaoTong University School of Medicine

Pancreatic fistula(PF) is the most frequent complication after distal pancreatectomy(DP).Prophylactic transpapillary pancreatic stenting(PTPS) has been proposed recently for the prevention of PF after *** this meta-analysis,a comprehensive search was performed in the PubMed,Embase,and Cochrane Library *** analyzing the results of PTPS in DP were considered eligible for this *** analyzed outcome variables included PF rate,postoperative morbidity,non-PF-related complications,mortality,operation duration,and hospital *** studies with 200 patients were included in this *** one was a randomized controlled trial(RCT).The results showed that PTPS was associated with less PF formation(odds ratio,0.45;95% confidence interval [CI],0.22–0.94;P = 0.03) and shorter hospital stay(mean difference,– 6.31;95% CI,– 6.99 to – 5.62;P < 0.00001).There was no significant difference in terms of the other *** conclusion,current evidence indicates that PTPS could reduce PF incidence and hospital stay after DP,without increasing other complications or operative ***,the evidence is not solid,because the single RCT conflicted with the other three retrospective ***,considering the limitation,more well-designed RCTs on this topic are needed in the future.

关键词： pancreatic fistula distal pancreatectomy pancreatic stent meta-analysis

Effect of isoflurane on expression of NR4A1 in hypoxic pulmonary artery

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Journal of Hainan Medical University 2019年第17期25卷 25-28页

作者： Rong Liu Zhi-Yong Xu hao weng Ding-Ding Huang Department of Anesthesiology Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus Shanghai Fengxian District Central Hospital Shanghai 201499 China

Objective: To investigate the effects of emulsified isoflurane on hypoxic pulmonary artery endothelial cells (HPAECs) injury and orphan nuclear receptor subfamily 4A1 (NR4A1) expression. Methods: HPAECs were divided into normal control group, model group and test group. Normal control group was cultured under normoxia. Cells in model group and test group were treated in a hypoxic chamber with oxygen concentration of about 3% (95% N2+ 5% CO2) for 2 h. The final concentration of 1 mmol·L-1 emulsified isoflurane was added to the test group, and 30% Intralipid? was added to the normal control group and the model group. MTT method was used to detect cell proliferation, Hoechst 33258 nuclear staining was used to detect cell apoptosis, Griess method was used to detect the production of NO in cell supernatant, and real-time fluorescence quantitative PCR (q-RT-PCR) was used to detect the expression of NR4A1 in cells. Results: After 12 h of intervention, the cell viability of normal control group, model group and test group were (98.45±2.41)%, (15.46±2.69)%, (79.52±4.16)%, the apoptosis rate were (2.51±0.36)%, (50.12±3.36)%, (22.15±3.42)%respectively, the concentration of NO in the culture supernatant were (59.52±4.1) μmol·L^-1, (25.16±4.85) μmol·L^-1, (43.58±6.19) μmol·L^-1, and the relative expression of NR4A1 were 1.00±0.09, 5.89±0.41, 2.39±0.24, respectively. The difference was statistically significant (P<0.05). Conclusion: Emulsified isoflurane can promote the proliferation, inhibit apoptosis and increase NO production of hypoxic HPAECs. NR4A1 may be involved in the endogenous protective mechanism of endothelial cell injury after hypoxia.

关键词： Emulsified isoflurane Hypoxia Endothelial cell injury Proliferation Apoptosis Orphan nuclear receptor subfamily 4A1

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思想政治教育融入大学生创新创业教育的路径

思想政治教育研究 2020年第1期36卷 152-155页

作者：翁灏东北师范大学教育学部

全球正孕育着新一轮科技产业变革,在中国经济发展形势从要素驱动、投资驱动转向创新驱动的国家战略背景下,大学生创新创业教育深度探索与发展将成为重要的研究内容。在分析大学生创新创业教育现状和阻滞因素的基础上,阐明了创新创业教... 详细信息

全球正孕育着新一轮科技产业变革,在中国经济发展形势从要素驱动、投资驱动转向创新驱动的国家战略背景下,大学生创新创业教育深度探索与发展将成为重要的研究内容。在分析大学生创新创业教育现状和阻滞因素的基础上,阐明了创新创业教育过程中融入思想政治教育的必要性和着力点,提出了构建思想政治教育融入创新创业过程的路径和方法,实现大学生创新创业教育与大学生课程教育、思想政治教育、第二课堂教育、校企实践平台体系的协同育人机制。

关键词：思想政治教育创新创业大学生融入路径

Duct-to-duct biliary reconstruction after radical resection of Bismuth Ⅲ a hilar cholangiocarcinoma

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World Journal of Gastroenterology 2013年第15期19卷 2441-2444页

作者： Wen-Guang Wu Jun Gu Ping Dong Jian-Hua Lu Mao-Lan Li Xiang-Song Wu Jia-Hua Yang Lin Zhang Qi-Chen Ding hao weng Qian Ding Ying-Bin Liu Depatment of General Surgery Xinhua HospitalAffiliated to School of MedicineShanghai Jiaotong University

At present, radical resection remains the only effective treatment for patients with hilar cholangiocarcinoma. The surgical approach for R0 resection of hilar cholangiocarcinoma is complex and diverse, but for the biliary reconstruction after resection, almost all surgeons use Roux-en-Y hepaticojejunostomy. A viable alternative to Roux-en-Y reconstruction after radical resection of hilar cholangiocarcinoma has not yet been proposed. We report a case of performing duct-to-duct biliary reconstruction after radical resection of Bismuth Ⅲa hilar cholangiocarcinoma. End-to-end anastomosis between the left hepatic duct and the distal common bile duct was used for the biliary reconstruction, and a singlelayer continuous suture was performed along the bile duct using 5-0 prolene. The patient was discharged favorably without biliary fistula 2 wk later. Evidence for tumor recurrence was not found after an 18 mo follow- up. Performing bile duct end-to-end anastomosis in hilar cholangiocarcinoma can simplify the complex digestive tract reconstruction process.

关键词： Hilar cholangiocarcinoma Biliary reconstruction Duct-to-duct Radical resection Digestive tract reconstruction Hepaticojejunostomy Bile duct anastomosis

Application of single balloon enteroscopy-assisted therapeutic endoscopic retrograde cholangiopancreatography in patients after bilioenteric Roux-en-Y anastomosis: Experience of multi-disciplinary collaboration

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World Journal of Gastroenterology 2019年第36期25卷 5505-5514页

作者： Wen-Guang Wu Lu-Cui Qin Xiao-Ling Song Ming-Ning Zhao Wen-Jie Zhang Jun Gu hao weng Ying-Bin Liu Yi Zhang Chun-Ying Qu Lei-Ming Xu Xue-Feng Wang Department of General Surgery and Laboratory of General Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092 China Department of Operating Room Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092 China Shanghai Key Laboratory of Biliary Tract Disease Research Shanghai 200092 China Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092 China

BACKGROUND Bilioenteric Roux-en-Y anastomosis is one of the most complicated approaches for reconstructing the gastrointestinal tract, and endoscopic retrograde cholangiopancreatography (ERCP) is technically challenging in patients after bilioenteric Roux-en-Y anastomosis. The optimal endoscopic strategies for such cases remain unknown. AIM To explore the feasibility and effectiveness of single balloon enteroscopy-assisted (SBE-assisted) therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis based on multi-disciplinary collaboration between endoscopists and surgeons as well as report the experience from China. METHODS This is a single center retrospective study. All of the SBE-assisted therapeutic ERCP procedures were performed by the collaboration between endoscopists and surgeons. The operation time, success rate, and complication rate were calculated. RESULTS Forty-six patients received a total of 64 SBE-assisted therapeutic ERCP procedures, with successful scope intubation in 60 (93.8%) cases and successful diagnosis in 59 (92.2%). All successfully diagnosed cases received successful therapy. None of the cases had perforation or bleeding during or after operation, and no post-ERCP pancreatitis occurred. CONCLUSION Based on multi-disciplinary collaboration, SBE-assisted therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis is relatively safe and effective and has a high success rate.

关键词： Bilioenteric Roux-en-Y anastomosis Single balloon enteroscopy Multidisciplinary cooperation Hepaticojejunostomy Pancreaticoduodenectomy