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第十三届全国植物基因组学大会

作者： hao wu,liang gao,yongjun lin~* national key laboratory of corp genetic improvement,natonal center of plant gene research(wuhan),huazhong agricultural university,wuhan 430070,china

As a staple for over 1 billion people in china,rice yield is damaged by over 350 insect *** various kinds of strategies are used to control pests,the losses are still ***(RNA interference) is believed to have great potential in pest controlling,but the lack of genetic material vastly limits the application of this *** Rice Pest Transcriptome DataBase(RPTDB) is a

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Development of maker-free insect-resistant transgenic rice by Agrobacterium-mediated co-transformation

Development of maker-free insect-resistant transgenic rice b...

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第十三届全国植物基因组学大会

作者： Fei ling,yongjun lin~* national key laboratory of corp genetic improvement,natonal center of plant gene research(wuhan),huazhong agricultural university,wu Han 430070,china

Agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants.A vector p1300Z-1C containing a synthetic Cry1C gene driven by maize ubiquitin promoter and pCAMBIA1301 harbouring hygromycin phosphotransferase gene(hpt) were introduced into Minghui 86(Oryza sativa L),an elite indica restorer *** independent transformants containing both the Cry1C

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Functional characterisation of OSLY15,which regulates rice stem and panicle growth

Functional characterisation of OSLY15,which regulates rice s...

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第十三届全国植物基因组学大会

作者： Yu Liu,yongjun lin~* national key laboratory of corp genetic improvement,natonal center of plant gene research(wuhan),huazhong agricultural university,wuhan 430070,P.r.china

plants have developed sophisticated signaling pathways to regulate their normal growth.A gene termed OsLY15 was first selected from our laboratory microarray ***,OsLY15 were showed to be specially expressed in stem and panicle according to GUS assay using transgenic plants harboring OsLY15.:GUS,and the results were confirmed by qRT-PCR using specific primers of OsL ***,

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The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice

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Journal of Integrative plant Biology 2018年第2期60卷 173-188页

作者： Yu Liu Chunjue Xu Yanfen Zhu lina Zhang Taiyu Chen Fei Zhou hao Chen yongjun lin national key laboratory of Crop genetic improvement and national center of plant gene research (wuhan) Huazhong Agricultural UniversityWuhan 430070 China Shenzhen Institute of Molecular Crop Design Shenzhen 518107 China

Calcium-dependent protein kinases（CPKs）are serine/threonine protein kinases that function in plant stress responses. Although CPKs are recognized as key messengers in signal transduction, the specific roles of CPKs and the molecular mechanisms underlying their activity remain largely unknown. Here, we characterized the function of Os CPK_（24）, a cytosol-localized calciumdependent protein kinase in rice. Os CPK_（24） was universally and highly expressed in rice plants and was induced by cold treatment. Whereas Os CPK_（24） knockdown plants exhibited increased sensitivity to cold compared to wild type（WT）, Os CPK_（24）-overexpressing plants exhibited increased cold tolerance. plants overexpressing Os CPK_（24） exhibited increased accumulation of proline（an osmoprotectant） and glutathione（an antioxidant） and maintained a higher GSH/GSSG（reduced glutathione to oxidized glutathione） ratio during cold stress compared to WT. In addition to these effects in response to cold stress, we observed the kinase activity of Os CPK_（24） varied under different calcium concentrations. Further,Os CPK_（24） phosphorylated Os Grx_（10）, a glutathionedependent thioltransferase, at rates modulated by changes in calcium concentration. Together, our results support the hypothesis that Os CPK_（24） functions as a positive regulator of cold stress tolerance in rice, a process mediated by calcium signaling and involving phosphorylation and the inhibition of Os Grx_（10） to sustain higher glutathione levels.

关键词： CPK Figure The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice

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