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From static to dynamic:live observation of the support system after ischemic stroke by two photon-excited fluorescence laser-scanning microscopy

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Neural Regeneration Research 2023年第10期18卷 2093-2107页

作者： Xuan Wu Jia-Rui Li Yu Fu Dan-Yang Chen hao nie Zhou-Ping Tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Geriatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is one of the most common causes of mortality and disability worldwide.However,treatment efficacy and the progress of research remain unsatisfactory.As the critical support system and essential components in neurovascular units,glial cells and blood vessels(including the bloodbrain barrier)together maintain an optimal microenvironment for neuronal function.They provide nutrients,regulate neuronal excitability,and prevent harmful substances from entering brain tissue.The highly dynamic networks of this support system play an essential role in ischemic stroke through processes including brain homeostasis,supporting neuronal function,and reacting to injuries.However,most studies have focused on postmortem animals,which inevitably lack critical information about the dynamic changes that occur after ischemic stroke.Therefore,a high-precision technique for research in living animals is urgently needed.Two-photon fluorescence laser-scanning microscopy is a powerful imaging technique that can facilitate live imaging at high spatiotemporal resolutions.Twophoton fluorescence laser-scanning microscopy can provide images of the whole-cortex vascular 3D structure,information on multicellular component interactions,and provide images of structure and function in the cranial window.This technique shifts the existing research paradigm from static to dynamic,from flat to stereoscopic,and from single-cell function to multicellular intercommunication,thus providing direct and reliable evidence to identify the pathophysiological mechanisms following ischemic stroke in an intact brain.In this review,we discuss exciting findings from research on the support system after ischemic stroke using two-photon fluorescence laser-scanning microscopy,highlighting the importance of dynamic observations of cellular behavior and interactions in the networks of the brain’s support systems.We show the excellent application prospects and advantages of two-photon fluorescence laser-scanning microscopy and predict future research develo

关键词： astrocytes blood-brain barrier calcium signaling glymphatic system ischemic stroke microglia network remodel two-photon fluorescence laser-scanning microscopy vessels

Introducing electrolytic electrochemical polymerization for constructing protective layers on Ni-rich cathodes of Li-ion batteries

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Rare Metals 2024年第6期43卷 2536-2545页

作者： Lin-Tao Dou Bei Li hao-Long nie Dong-Dong Xiao Chao-Qun Shang Xiao-Min Wang Zhan-Hui Zhang Katerina E.Aifantis Pu Hu School of Materials Science and Engineering Wuhan Institute of Technology Huairou Division Institute of PhysicsChinese Academy of Sciences Department of Mechanical and Aerospace Engineering University of Florida

LiNi0.8Co0.1Mn0.1O2（NCM811） is the most promising cathode for high-energy Li-ion batteries,despite its poor cycling stability that originates from the reactions that occur with the electrolyte.Herein,to solve this interfacial issue,a facile electrolytic electrochemical polymerization process was introduced in this paper,and a uniform conductive electrolyte interface（polyaniline） was successfully constructed on the surface of the NCM811 porous electrode（PANI-NCM）,which facilitated the charge transfer during charge/discharge.The side reactions at the interface between the cathode and the electrolyte are suppressed,and thereby,the cycling performance and rate capability are considerably improved.PANI-NCM delivers an initial capacity of 157.2 mAh·g-1as well as excellent cyclability（capacity retention of 88% after 500 cycles at 2C）,whereas the capacity of the bare NCM811 has dropped to 31.3 mAh·g-1.In addition,polypyrrole and polythiophene also can be formed through electrolytic electrochemical polymerization process,which provides a practicable tactic to modify the interfacial stability of cathodes for high-energy Li-ion batteries.

关键词： Lithium-ion battery Ni-rich cathode In-situ polymerization Conductive polymer Electrochemical performance

Improving tensile-shear properties of friction stir lap welded dissimilar Al/Mg joints by eliminating hook defect and controlling interfacial reaction

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Chinese Journal of Aeronautics 2023年第7期36卷 257-267页

作者： Yuqing MAO Ping YANG Wenyan ZHANG Ning LI hao nie Danyang LIN Liming KE National Defence Key Discipline Laboratory of Light Alloy Processing Science and Technology Nanchang Hangkong UniversityNanchang 330063China

To improve tensile-shear properties of fiction stir lap welded(FSLW) dissimilar Al/Mg joints, pin-tip profiles were innovatively designed and welding speed was optimized, and effects of them on formation, interface microstructure and mechanical properties of different FSLW joints were investigated. With increasing the welding speed, the tensile-shear load of FSLW joints produced by three pins presents an increasing firstly and then decreasing trend. Compared with Rpin, the hook and hole defect in the joints made by S-pin and T-pin are eliminated owing to additional eccentric force. Moreover, the joints obtained by T-pin at 75 mm/min have the highest tensile-shear load, and a maximum value of 3.425 kN is produced, which increases by 96.8%.Meanwhile, the pin-tip profile improves significantly the interface reaction depending on the welding temperature. For R-pin, thick brittle intermetallic compounds of about 6.9 μm Al3Mg2and 13.3 μm Al12Mg17layers at the welding interface derived from diffusion reaction are formed, resulting in continuous cracks. However, using T-pin can raise the interface temperature, and which makes the interface liquefy locally to generate only 2.2 μm Al3Mg2layer and dispersive(Al12-Mg17+Mg) eutectic structure. This can release high residual stress and remove welding crack, consequently enhancing the interface properties of T-pin joints.

关键词： Dissimilar Al/Mg alloys Friction stir lap welding Hook defect Interface microstructure Tensile-shear properties

局部强冷作用下厚板铝合金/镁合金搅拌摩擦焊界面金属间化合物的析出行为

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金属学报 2024年第6期60卷 777-788页

作者：徐洋柯黎明聂浩夏春刘强陈书锦南昌航空大学轻合金加工科学与技术国防重点学科实验室南昌330063 江苏科技大学材料科学与工程学院镇江212003

厚板铝合金/镁合金在搅拌摩擦焊(FSW)时接头界面沿板厚方向存在严重的温度分布差异问题,导致接头界面组织分布极不均匀,接头成形较为困难。减小接头界面沿板厚方向温差成为改善厚板铝合金/镁合金FSW接头成形的关键之一。本工作选取15 m... 详细信息

厚板铝合金/镁合金在搅拌摩擦焊(FSW)时接头界面沿板厚方向存在严重的温度分布差异问题,导致接头界面组织分布极不均匀,接头成形较为困难。减小接头界面沿板厚方向温差成为改善厚板铝合金/镁合金FSW接头成形的关键之一。本工作选取15 mm厚的5A06-H112铝合金和AZ31B-O镁合金板材进行异种材料FSW,采用液氮喷洒于焊缝上表面进行局部强冷,利用EBSD和TEM获得焊接接头物相分布和晶粒取向散布,研究了铝合金/镁合金接头搅拌区(SZ)两侧界面处金属间化合物(IMCs)的析出行为。结果表明,在空冷和液氮冷却2种条件下SZ的铝合金侧主要析出Al_(3)Mg_(2)相;镁合金侧主要析出Al_(12)Mg_(17)相,且在上部界面处与Mg发生了共晶反应;焊缝表面局部强冷,降低了接头各位置的峰值温度和高温停留时间,有效抑制了IMCs的析出和低熔点共晶的形成。不施加表面冷却时,铝合金侧界面上部和中部附近的SZ中主要析出Al_(3)Mg_(2)相,底部SZ中则为细小的等轴铝合金晶粒;镁合金侧界面上部的SZ中则主要析出了Al_(12)Mg_(17)相,并与Mg形成低熔点共晶,且在共晶区与SZ中的铝合金之间析出Al_(3)Mg_(2)薄层,中部和下部SZ与镁合金之间析出了界线分明、由Al_(3)Mg_(2)层和Al_(12)Mg_(17)层组成的IMCs叠层,且中部的IMCs叠层总厚度远大于峰值温度较低的底部处IMCs叠层厚度,其中Al_(3)Mg_(2)层厚度减薄更为明显。当在焊缝表面施加液氮冷却时,铝合金侧界面上部的SZ中除了析出Al_(3)Mg_(2)相外,还有少部分Al_(12)Mg_(17)和铝合金晶粒,而界面中部和底部SZ中均为等轴状的铝合金晶粒;在镁合金侧界面处,各部位IMCs析出行为与不施加表面冷却时相似,但界面上部析出的Mg+Al_(12)Mg_(17)共晶层和Al_(3)Mg_(2)层、中部和下部SZ界面IMCs叠层总厚度明显减小,Al_(3)Mg_(2)薄层厚度降低更为显著。应变速率对IMCs的析出有显著影响,表现为界面层实际厚度远大于由扩散定律计算的理论厚度。

关键词：搅拌摩擦焊厚板铝合金/镁合金接头金属间化合物局部强冷

O-GlcNAc修饰与疾病病理学的联系（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第5期20卷 437-448页

作者： hao nie Wen YI MOE Laboratory of Biosystems Homeostasis&Protection College of Life SciencesZhejiang University

O-连接的N-乙酰葡萄糖胺(O-GlcNAc)修饰是一种发生于蛋白质丝氨酸或苏氨酸残基上的蛋白翻译后修饰,它能动态地发生在细胞的任何部位,如细胞质、线粒体、细胞核等。O-GlcNAc修饰的供体尿苷二磷酸-N-乙酰葡萄糖胺(UDP-GlcNAc)是己糖胺生... 详细信息

O-连接的N-乙酰葡萄糖胺(O-GlcNAc)修饰是一种发生于蛋白质丝氨酸或苏氨酸残基上的蛋白翻译后修饰,它能动态地发生在细胞的任何部位,如细胞质、线粒体、细胞核等。O-GlcNAc修饰的供体尿苷二磷酸-N-乙酰葡萄糖胺(UDP-GlcNAc)是己糖胺生物合成途径(HBP)的终产物。GlcNAc在蛋白上的添加和移除分别需要O-GlcNAc转移酶(OGT)和O-GlcNAc水解酶(OGA)的介导。被O-GlcNAc修饰的蛋白包括转录因子、代谢酶、细胞信号传导因子等。O-GlcNAc通过调控这些蛋白的功能参与到许多重要的生物进程中,如转录、翻译、代谢、信号传导、自噬等。此外,O-GlcNAc修饰的失调还与许多重要疾病息息相关,包括癌症、糖尿病、神经退行性疾病和心血管疾病。更好地了解O-GlcNAc修饰在这些疾病生理病理学进程中所起的作用将有助于开发新的治疗策略。本文介绍了O-GlcNAc与疾病病理联系的最新研究,并揭示O-GlcNAc可作为潜在的临床治疗靶点。

关键词： O连接的N-乙酰葡萄糖胺(O-GlcNAc) 癌症糖尿病神经退行性疾病心血管疾病

Naringenin protects against isoniazid- and rifampicininduced apoptosis in hepatic injury

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World Journal of Gastroenterology 2016年第44期22卷 9775-9783页

作者： Chao Wang Rui-Qin Fan Yan-Xiang Zhang hao nie Kan Li Department of Laboratory Medicine Medical School Yangtze University Clinical and Molecular Immunology Research Center Yangtze University Department of Hepatic Disease the Second Hospital of Jingzhou

AIM To explore the protective effects and mechanisms of naringenin(NRG) on hepatic injury induced by isoniazid(INH) and rifampicin(RIF).METHODS Male mice were randomly divided into four groups and treated for 14 d as follows: normal control group was administered intragastrically with normal saline solution alone; model group was administered intragastrically with INH(100 mg/kg) and RIF(100 mg/kg); lowand high-dosage NRG pretreatment groups were administered intragastrically with different doses of NRG(50 or 100 mg/kg) 2 h before INH and RIF challenge. Mice were killed 16 h after the last dose of drug treatment to determine activity of serum transaminases. Oxidative stress was evaluated by measuring hepatic glutathione(GSH) and superoxide dismutase(SOD) and malondialdehyde(MDA) levels. Histopathological changes in hepatic tissue were observed under the optical microscope. Hepatocyte apoptosis was measured by TUNEL assay and caspase-3 activation. Expression of Bcl-2 and Bax in liver was determined by western blot.RESULTS Both low- and high-dosage NRG pretreatment obviously alleviated serum levels of alanine aminotransferase and aspartate aminotransferase, liver index, hepatic MDA content, and increased hepatic GSH content and SOD activity compared with the INH and RIF-treated group(44.71 ± 8.15 U/L, 38.22 ± 6.64 U/L vs 58.15 ± 10.54 U/L; 98.36 ± 14.78 U/L, 92.41 ± 13.59 U/L vs 133.05 ± 19.36 U/L; 5.34% ± 0.26%, 4.93% ± 0.25% vs 5.71% ± 0.28%; 2.76 ± 0.67 nmol/mgprot, 2.64 ± 0.64 nmol/mgprot vs 4.49 ± 1.12 nmol/mgprot; 5.91 ± 1.31 mg/gprot, 6.42 ± 1.42 mg/gprot vs 3.11 ± 0.73 mg/gprot; 137.31 ± 24.62 U/mgprot, 148.83 ± 26.75 U/mgprot vs 102.34 ± 19.22 U/mgprot; all P < 0.01 or 0.05). Histopathological evaluation showed obvious necrosis and inflammatory cell infiltration in liver of mice administered INH and RIF; however, mice pretreated with NRG showed minor hepatic injury. In addition, INH and RIF resulted in hepatocyte apoptosis, and NRG pretreatment dramatically suppressed INHand RIF-induced hepatocytes apoptosi

关键词： Naringenin Isoniazid Rifampicin Oxidative stress Apoptosis Hepatic injury

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补体凝集素与呼吸系统疾病

中国免疫学杂志 2024年第3期40卷 641-644,650页

作者：王世雄聂浩张昊川黄河安徽医科大学深圳二院临床学院深圳市第二人民医院呼吸与危重症医学科深圳518035 长江大学医学部荆州434023

补体凝集素途径是补体系统激活的一种途径,纤维胶凝蛋白(ficolin)与甘露糖结合凝集(MBL)是激活免疫补体途径的一类起始物,两者结构及功能极其相似,可通过识别病原表面的某些物质,激活补体,加强调理吞噬作用或直接杀灭和溶解相关病原,从... 详细信息

补体凝集素途径是补体系统激活的一种途径,纤维胶凝蛋白(ficolin)与甘露糖结合凝集(MBL)是激活免疫补体途径的一类起始物,两者结构及功能极其相似,可通过识别病原表面的某些物质,激活补体,加强调理吞噬作用或直接杀灭和溶解相关病原,从而在免疫系统中尤其在抗感染方面发挥重要作用。呼吸系统疾病多以感染性疾病为主。近年来,也有相关研究报道ficolin/MBL与众多其他呼吸系统疾病具有相关性,本文就ficolin/MBL与呼吸系统疾病的相关研究进行综述,以期为相关研究提供理论支撑。

关键词：纤维胶凝蛋白甘露糖结合凝集素补体呼吸系统疾病

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自动化技术在智能家居系统中的应用与实践

电视技术 2024年第2期48卷 225-228页

作者：聂豪重庆科技大学重庆401331

智能家居系统以其能够提高生活便利性和节能效果的特点,逐渐成为人们日常生活的组成部分。自动化技术作为实现智能家居系统核心功能的重要手段,在安全、能源管理、娱乐等方面的应用已经取得了显著进展。研究旨在探讨自动化技术在智能家... 详细信息

智能家居系统以其能够提高生活便利性和节能效果的特点,逐渐成为人们日常生活的组成部分。自动化技术作为实现智能家居系统核心功能的重要手段,在安全、能源管理、娱乐等方面的应用已经取得了显著进展。研究旨在探讨自动化技术在智能家居系统中的具体应用与实践,深入了解其对家庭生活的影响。研究发现,自动化技术在智能家居系统中广泛应用于安全监控、环境控制、能源管理和家庭娱乐等方面。通过智能传感器、语音识别、远程控制等技术手段的整合,智能家居系统能够实现对家庭设备的智能化管理,提高家庭生活的便利性。

关键词：自动化技术智能家居系统智能化控制

Single-cell RNA-Seq analysis identified kidney progenitor cells from human urine

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Protein & Cell 2021年第4期12卷 305-312页

作者： Yujia Wang Yu Zhao Zixian Zhao Dandan Li hao nie Yufen Sun Xiaobei Feng Ting Zhang Yu Ma Jing nie Guangyan Cai Xiangmei Chen Wei Zuo East Hospital School of MedicineTongji UniversityShanghai 200092China Regend Therapeutics Co.Ltd. Zhangjiang Hi-tech ParkShanghai 201203China Ningxia Medical University Yinchuan 750004China The First Affiliated Hospital Guangzhou Medical UniversityGuangzhou 510120China Ruijin Hospital School of MedicineShanghai Jiaotong UniversityShanghai 200025China State Key Laboratory of Organ Failure Research National Clinical Research Center of Kidney DiseaseKey Laboratory of Organ Failure ResearchDivision of NephrologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China National Clinical Research Center for Kidney Diseases State Key Laboratory of Kidney DiseasesChinese PLA General HospitalBeijing 100039China

Dear Editor,Urine passes through the entire kidney and urinary tract system starting from the glomerulus and ending to the urethra.Cells in the kidney and urinary tract could be exfoliated from the epithelium into the urine,while leukocyte could infiltrate from the local tissue into the urine,which makes the urine a useful subject for clinical evaluation of relevant diseases.

关键词： urine kidney urinary