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Preliminary Application of WCX Magnetic Bead-Based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Analyzing the Urine of Renal Clear Cell Carcinoma

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Chinese Medical Sciences Journal 2017年第4期32卷 248-252页

作者： Dexin Dong Zhigang Ji Hanzhong Li Weigang Yan Yushi Zhang Department of Urology Peking Union Medical College HospitalChinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China

Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC.Methods Eleven newly diagnosed patients (10 males and 1 female, aged 46-78, mean 63 years) of renal clear cell carcinoma by biopsy and 10 healthy volunteers (all males, aged 25-32, mean 29.7 years) were enrolled in this study. Urine samples of the RCCC patients and healthy controls were collected in the morning.Weak cation exchange (WCX) bead-based MALDI-TOF MS technique was applied in detecting differential protein peaks in the urine of RCCC. ClinProTools2.2 software was utilized to determine the characteristic proteins in the urine of RCCC patients for the predictive model of RCCC.Results The technique identified 160 protein peaks in the urine that were different between RCCC patients and health controls; and among them, there was one peak (molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic algorithms and the support vector machine, we screened out 13 characteristic protein peaks for the predictive model.Conclusions The application of WCX magnetic bead-based MALDI-TOF MS in detecting differentiallyexpressed proteins in urine may have potential value for the early diagnosis of RCCC.

关键词： renal clear cell carcinoma proteomics magnetic beads urine diagnosis model

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Application of diatomite as an effective polysulfides adsorbent for lithium-sulfur batteries

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Journal of Energy Chemistry 2017年第6期26卷 1267-1275页

作者： Zhong li Nan Zhang Yubao Sun hanzhong Ke Hansong Cheng Sustainable Energy Laboratory Faculty of Material Science and ChemistryChina University of Geosciences(Wuhan)Wuhan 430074HubeiChina

The lithium–sulfur batteries show the great potential to be the most promising candidate for high energy applications. However, the shuttling of soluble polysulfides deteriorates the battery performance tremendously. To suppress the diffusion of soluble polysulfides, diatomite that has abundant natural three-dimensional ordered pores is incorporated into the cathode to trap polysulfides. The composite cathode material(S-DM-AB for short), including sulfur(S), diatomite(DM), and acetylene black(AB) is prepared by an impregnation method. For comparison, another composite cathode material(S-AB for short) including sulfur and acetylene black is also prepared by the same method. The battery with S-DMAB composite cathode material delivers a discharge capacity of 531.4 m Ah/g after 300 cycles at 2 C with a capacity retention of 51.6% at room temperature. By contrast, the battery with S-AB composite cathode material delivered a capacity of only 196.9 m Ah/g with a much lower capacity retention of 18.6% under the same condition. The addition of diatomite in the cathode is proved to be a cheap and effective way to improve the life time of the lithium sulfur batteries.

关键词： lithium–sulfur batteries Diatomite Adsorbent Cycle performance

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经尿道手术机器人的性能评价和应用研究

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中华泌尿外科杂志 2023年第4期44卷 301-306页

作者：连鹏鹄李汉忠中国医学科学院北京协和医院泌尿外科北京100710

目的评价新型主从操作式经尿道手术机器人系统的性能,验证其安全性和有效性。方法2021年9月北京协和医院2名泌尿外科医生(A和B)应用经尿道手术机器人样机进行仿真人体组织模型实验。本研究采用的经尿道手术机器人系统包括:主端控制平台... 详细信息

目的评价新型主从操作式经尿道手术机器人系统的性能,验证其安全性和有效性。方法2021年9月北京协和医院2名泌尿外科医生(A和B)应用经尿道手术机器人样机进行仿真人体组织模型实验。本研究采用的经尿道手术机器人系统包括:主端控制平台、从端手术平台和末端执行器。主端控制平台采用Omega7力反馈主手作为主控制器,自由度包括:上下平移、左右平移、前后平移、末端旋转、末端俯仰、末端摆动、末端操作。从端手术平台采用Med7七自由度医疗协作机械臂,通过末端执行器操作电切镜。末端执行器采用模块化设计,最大化兼容现有手术器械。2名医生均分别常规组装电切镜和经尿道手术机器人各20次,计算组装时间。常规组装电切镜时间包括电切镜安装、连接镜头和光源后,医生手持电切镜进入实验模块的时间。组装手术机器人时间包括将电切镜与末端执行器安装,并连接镜头和光源后进入实验模块的时间。2名医生分别进行25次模拟前列腺电切手术和5次模拟膀胱电切手术。将猪的小肠、心脏和胃缝合构建模拟人体的尿道、前列腺和膀胱结构:尿道(猪小肠)长16~18cm,前列腺(猪心脏)大小约5cm×5cm×6cm,膀胱(猪胃)容量250~300ml。将模型置于3D打印套盒中,模拟尿道与硅胶阴茎贴合。模拟前列腺电切手术:医生于主端控制平台操作手柄,通过人机交互控制从端执行器,直视下围绕不动点切除“前列腺”,模拟标准经尿道前列腺切除术,切除范围为从模拟膀胱颈至模拟前列腺尖部,切除两侧叶和中叶。每次手术时间40min,记录切除组织重量。模拟经尿道膀胱电切手术:每次手术需切除模拟膀胱的三角区、两侧壁和顶部区域,记录是否发生穿孔。通过手术对机器人的主从操作距离准确度、操作姿态准确度、主从操作姿态重复度、不动点准确度、主从控制启动延迟时间和主从控制跟随延迟时间、机械臂摆动范围、极限位点等指标进行验证。结果经尿道手术机器人性能测试结果显示,末端执行器定位精度<0.5mm,主从操作距离准确度≤0.5mm,重复性距离≤0.2mm;主从操作姿态准确度角度a≤0.30°,角度b≤0.30°,角度c≤0.15°;不动点准确度≤0.6mm,机械臂最大活动空间为半径(1493±5)mm的半球形空间。主从控制启动延迟时间和主从控制跟随延迟时间均≤100ms。从端机械臂末端在运动中受到外力碰撞时,系统可自动停止机械臂运动,此时外力为(70±7)N;不动点设置范围30~170mm。医生A和医生B组装经尿道手术机器人时间分别为(111.35±57.88)s和(111.70±58.30)s(P=0.996),常规组装电切镜时间分别为(44.90±4.89)s和(44.90±5.16)s(P=0.679),2名医生间比较差异均无统计学意义;2名医生的机器人组装时间均多于常规组装时间,差异均有统计学意义(P=0.001,P=0.001)。随组装次数增加,机器人组装时间进行性下降,5次组装后时间稳定≤120s。医生A和医生B切除的前列腺组织重量分别为(43.60±12.42)g和(43.45±12.63)g,差异无统计学意义(P=0.954)。模拟膀胱电切手术中,机器人系统可顺利完成模拟膀胱的三角区、两侧壁和顶部组织切除。手术实验中系统运行流畅,未发生机械故障,无模块损伤、穿孔等并发症发生。结论经尿道手术机器人能够安全、有效地完成经尿道电切手术。

关键词：经尿道手术机器人远程运动中心人机交互主从控制

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Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening

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Virologica Sinica 2022年第3期37卷 459-461页

作者： Yuan Zhang Chunjie li Xianliang Ke Dan Luo Yan liu Quanjiao Chen hanzhong Wang Xiaohui Song Zhenhua Zheng Key Laboratory of Special Pathogens and Biosafety Center for Emerging Infectious DiseasesWuhan Institute of VirologyChinese Academy of SciencesWuhan430071China Department of Gastroenterology Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhan430015China Department of Obstetrics Wuhan Children's HospitalWuhan Maternal and Child Healthcare HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhan430015China University of the Chinese Academy of Sciences Beijing100049China

Dear Editor,The Coronavirus disease 2019(COVID-19)pandemic is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The genome of this virus encodes two overlapping polyproteins,pp1a and pp1ab(Wu et al.,2020;Zhou et al.,2020).The functional polypeptides are released from those two polyproteins by extensive proteolytic events.

关键词： protease drugs

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CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC

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BIOCELL 2024年第5期48卷 817-834页

作者： KAIWEN TIAN hanzhong CHEN QIANQIAN WANG FENGliAN JIANG CHUNXIANG FENG TENG li XIAOYONG PU YANliN TANG JIUMIN liU Department of Urology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou510080China

Background:The incidence of clear cell renal cell carcinoma(ccRCC)is globally high;however,despite the introduction of innovative drug therapies,there remains a lack of effective biomarkers for evaluating treatment response.Recently,Caspase recruiting domain-containing protein 11(CARD11)has garnered attention due to its significant association with tumor development and the immune system.Methods:The expression of CARD11 mRNA and protein in ccRCC were analyzed by public database and immunohistochemistry.The focus of this study is on the epigenomic modifications of CARD11,its expression of ccRCC immunophenotype,and its correlation with response to immunotherapy and targeted therapy.Furthermore,to investigate the mechanism of this molecule’s influence on different biological behaviors of cells,cell tests in vitro have been conducted to observe the impact of its expression level.Results:CARD11 expression was upregulated which may be mainly modified by body methylation and was correlated with poor prognosis in ccRCC.In the tumor microenvironment of ccRCC,CARD11 expression was positively correlated with increased T lymphocyte infiltration and increased expression of inhibitory immune checkpoints.Moreover,ccRCC patients with high CARD11 expression had a better response to immunotherapy and targeted therapy.The knockdown of CARD11 ultimately suppressed the proliferation,migration,and invasion capabilities of ccRCC cells while simultaneously enhancing tumor cell apoptosis.Conclusion:We identified CARD11 as a novel therapeutic biomarker for immunotherapy and targeted therapy in ccRCC.

关键词： Clear cell renal cell carcinoma Tumor microenvironment CARD11 Immune checkpoint inhibitor Tyrosine kinase inhibitor

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从2022年WHO分类看副神经节瘤与嗜铬细胞瘤相关概念的更新及解读

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中华泌尿外科杂志 2022年第11期43卷 807-811页

作者：张玉石李汉忠中国医学科学院北京协和医学院北京协和医院泌尿外科北京100730

2022年WHO发布了第五版神经内分泌肿瘤分类,在有关副神经节瘤和嗜铬细胞瘤(PPGL)的内容方面较2017年第四版有所更新。在第五版的PPGL分类系统中针对既往比较模糊和不明确的概念进行了重新定义,同时也提出了一些新的观点。本文在此基础... 详细信息

2022年WHO发布了第五版神经内分泌肿瘤分类,在有关副神经节瘤和嗜铬细胞瘤(PPGL)的内容方面较2017年第四版有所更新。在第五版的PPGL分类系统中针对既往比较模糊和不明确的概念进行了重新定义,同时也提出了一些新的观点。本文在此基础上结合国内临床现状,就有关更新内容进行介绍,包括副神经节瘤与嗜铬细胞瘤的概念演变;类副神经节瘤、复合副神经节瘤、肾上腺髓质增生、微小嗜铬细胞瘤等定义的准确解读;理解分子诊断及预后相关标志物;正确认识PPGL良恶性的定义及临床分期。通过对这些关键概念问题的探讨,使国内学者对PPGL中的一些概念进行统一,避免混肴,利于学术交流。

关键词：副神经节瘤嗜铬细胞瘤神经内分泌肿瘤分类

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Collagen fiber membrane-derived chemically and mechanically durable superhydrophobic membrane for high- performance emulsion separation

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Journal of Leather Science and Engineering 2021年第1期3卷 214-223页

作者： Huifang li Wan Zheng hanzhong Xiao Baicun Hao Yujia Wang Xin Huang Bi Shi National Engineering Research Center of Clean Technology in Leather Industry Sichuan University610065 ChengduP.R.China

Developing high-performance separation membrane with good durability is a highly desired while challenging issue.Herein,we reported the successful fabrication of chemically and mechanically durable superhydrophobic membrane that was prepared by embedding UiO-66 as size-sieving sites within the supramolecular fiber structure of collagen fiber membrane(CFM),followed by the polydimethylsiloxane(PDMS)coating.The as-prepared CFM/UiO-66(12)/PDMS membrane featured capillary effect-enhanced separation flux and homogeneous porous channels guaranteed high separation efficiency.When utilized as double-layer separation membranes,this new type of composite membranes separated various surfactant stabilized water-in-oil microemulsions and nanoemulsions,with the separation efficiency high up to 99.993%and the flux as high as 973.3 L m−2 h−1.Compared with commercial polytetrafluoro ethylene(PTFE)membrane,the advantage of the double-layer CFM/UiO-66(12)/PDMS membranes in separation flux was evident,which exhibited one order of magnitude higher than that of commercial PTFE membrane.The CFM/UiO-66(12)/PDMS membrane was acid-alkali tolerant,UV-aging resistant and reusable for emulsion separation.Notably,the CFM/UiO-66(12)/PDMS membrane was mechanically durable against strong mechanical abrasion,which was still capable of separating diverse water-in-oil emulsions after the abrasion with sandpaper and assembled as double-layer separation membranes.We anticipate that the combination of CFM and metal organic frameworks(MOFs)is an effective strategy for fabricating high-performance separation membrane with high mechanical and chemical durability.

关键词： collagen fiber membrane metal organic frameworks superhydrophobic composite membrane emulsion separation mechanical and chemical durability

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通用测试仪器流程控制软件设计

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计算机时代 2023年第7期 82-86页

作者：来韩忠李晓明浙江理工大学机械与自动控制学院浙江杭州310018

文章主要解决将自动化测试系统中的测试流程标准化的问题,首先设计并实现了一种基于XML的自动测试流程描述语言,然后基于该自动测试流程描述语言通过使用java编码的方式实现一个测试流程控制软件,用于读取自动测试流程描述语言控制整个... 详细信息

文章主要解决将自动化测试系统中的测试流程标准化的问题,首先设计并实现了一种基于XML的自动测试流程描述语言,然后基于该自动测试流程描述语言通过使用java编码的方式实现一个测试流程控制软件,用于读取自动测试流程描述语言控制整个测试流程,提高测试应用的开发效率。最后通过测试仪器进行实例测试检测该测试流程控制软件的可行性、实用性。

关键词： XML 测试仪器流程自动化自动测试

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Effects of bias voltage on coating structures and anticorrosion performances of PA-PVD Al coated NdFeB magnets

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Journal of Rare Earths 2021年第6期39卷 703-711,I0004页

作者： Yujie Cao Pengjie Zhang Wei Sun Weifeng Zhang hanzhong Wei Jiquan Wang Bingshan li Xiaofei Yi Guangqing Xu Yucheng Wu School of Materials Science and Engineering Hefei University of TechnologyHefei 230009China State Key Laboratory of Rare Earth Permanent Magnet Materials Earth-Panda Advance Magnetic Material Co.Ltd.Hefei 231500China BGRIMM Magnetic Materials and Technology(Fu Yang)Co. Ltd.Fuyang 236000China BGRIMM Technology Group Co. Ltd.Beijing 102600China

Al coated NdFeB magnets were prepared by plasma-assisted-physical-vapor-deposition(PA-PVD)method fo r enhancing the corro sion resistance.Mo rphologies and structures were characterized by an Xray diffractometer and a scanning electron microscope.Corrosion behaviors of the samples with different bias voltages were studied by electrochemical methods and neutral salt spray test,respectively.The bias voltage during the deposition process was optimized according to the coating structures and corrosion resistances.The density and flatness of Al coating increase with increasing the bias voltage,and at the same time the thickness decreases.The coating density and thickness turn to be stable when bias voltage is 1800 V.Also,the corrosion behaviors of PA-PVD Al coatings in different media,such as NaOH,HNO_(3) and NaCl solutions,were studied.The self-corrosion potentials(E_(corr)) of PA-PVD Al coatings almost keep constant in NaOH and HNO_(3) solutions with diffe rent concentrations.However,the self-corrosion current densities(J_(corr)) decrease with the increasing concentrations.E_(corr) shifts to negative potential and J_(corr)decreases gradually when increasing the concentration of NaCl solution.The corrosion mechanisms of Al coatings are discussed based on the corrosion behaviors in different media.

关键词： Physical vapor deposition Plasma assistance Al coating Sintered NdFeB Corrosion behavior Rare earths

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2016年前列腺癌诊治热点回顾

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中华外科杂志 2017年第1期55卷 59-62页

作者：李汉忠张玉石中国医学科学院北京协和医学院北京协和医院泌尿外科 100730

随着人口老龄化、饮食结构改变和医疗体检普及，近年来我国男性前列腺癌的发病率和死亡率呈上升趋势。近期前列腺癌研究领域的大量临床及基础研究从多方面对前列腺癌的临床诊治进行了深入探讨，为前列腺癌的临床诊治提供了新方向。本文... 详细信息

随着人口老龄化、饮食结构改变和医疗体检普及，近年来我国男性前列腺癌的发病率和死亡率呈上升趋势。近期前列腺癌研究领域的大量临床及基础研究从多方面对前列腺癌的临床诊治进行了深入探讨，为前列腺癌的临床诊治提供了新方向。本文就近一年来前列腺癌领域诊断和治疗方面重要的前沿进展进行回顾并归纳总结。

关键词：前列腺肿瘤诊断治疗

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