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Food Science and Human Wellness 2024年第1期13卷 146-153页

作者： Baifeng Fu Di Wu Shuzhen Cheng Xianbing Xu Ling Zhang Lishu Wang Heshanm REl-Seedi hanxiong liu Ming Du School of Food Science and Technology Dalian Polytechnic UniversityDalian 116034China Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China National Engineering Research Center of Seafood Dalian Polytechnic UniversityDalian 116034China Division of Hematology and Oncology Department of MedicineMedical College of WisconsinMilwaukee 53226USA Division of Pharmacognosy Department of Medicinal ChemistryBiomedical CentreUppsala UniversityUppsala SE-75123Sweden

Oyster(Crassostrea gigas),the main ingredient of oyster sauce,has a strong umami *** this study,three potential umami peptides,FLNQDEEAR(FR-9),FNKEE(FE-5),and EEFLK(EK-5),were identified and screened from the alcoholic extracts of the oyster using nano-HPLC-MS/MS analysis,i Umami-Scoring Card Method(i Umami-SCM)database and molecular docking(MD).Sensory evaluation and electronic tongue analysis were further used to confirm their *** threshold of the three peptides ranged from 0.38 to 0.55 mg/m *** with umami receptors T1R1/T1R3 indicated that the electrostatic interaction and hydrogen bond interaction were the main forces ***,the Phe592 and Gln853 of T1R3 were the primary docking site for MD and played an important role in umami *** with two Glu residues at the terminus had stronger umami,especially at the *** results contribute to the understanding of umami peptides in oysters and the interaction mechanism between umami peptides and umami receptors.

关键词： Oyster umami peptides iUmami-SCM Taste characterization Molecular docking

A comprehensive method to explore inhibitory kinetics and mechanisms of an anticoagulant peptide derived from Crassostrea gigas

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Food Science and Human Wellness 2022年第6期11卷 1491-1499页

作者： Shuzhen Cheng Di Wu hanxiong liu Xianbing Xu Beiwei Zhu Ming Du Department of Food Science and Engineering National Engineering Research Center of SeafoodDalian Polytechnic UniversityDalian 116034China Beijing Advanced Innovation Center for Food Nutrition and Human Health College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China

A comprehensive method was applied to evaluate the anticoagulant activity of a novel anticoagulant peptide(NAESLRK)derived from oyster(Crassostrea gigas).The anticoagulant peptide drastically reduced the extrinsic clotting activity and also impaired the intrinsic clotting activity *** with clotting data,the thrombin peak height was reduced to 84.7 nmol/L from 123.4 nmol/L,and thrombin generation time was delayed to 4.67 min from 4.42 min when the extrinsic trigger was *** inhibitory kinetics of FⅪa,FⅨa,FⅩa,FⅡa,and APC in a purified component system rationally explained the reduction of extrinsic clotting activity and impairment of thrombin *** the inhibition of FⅩa and FⅡa activity,the activation processes of FⅩand FⅡby intrinsic/extrinsic tenase complex and prothrombinase were also *** anticoagulant activity in the plasma system was the result of comprehensive inhibition of various *** research provided a novel method for anticoagulant evaluation and inhibitory mechanism of bioactive peptides from food products.

关键词： Oyster Anticoagulant peptide Inhibitory kinetics Coagulation factor Non-specific inhibitor

In vitro and in silico analysis of dual-function peptides derived from casein hydrolysate

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Food Science and Human Wellness 2021年第1期10卷 32-37页

作者： Maolin Tu Xinyu Qiao Cong Wang hanxiong liu Shuzhen Cheng Zhe Xu Ming Du School of Food Science and Technology National Engineering Research Center of SeafoodCollaborative Innovation Center of Seafood Deep ProcessingDalian Polytechnic UniversityDalian 116034China

There is no study on food-derived peptide with both anticoagulant and angiotensin I-converting enzyme inhibitory (ACEI) activities yet. In this work, the anticoagulant and ACEI activities of the casein hydrolysates released by pepsin digestion were evaluated for the first time to the best of our knowledge. Results indicated that the casein hydrolysate exhibited potent anticoagulant activity by prolonging the thrombin time (TT) and the activated partial thromboplastin time (APTT). Compared with control samples, at 10 mg/mL, the TT and APTT of casein hydrolysate were 186.0 % ± 6.6 % and 163.5 % ± 7.4 %, respectively. The casein hydrolysate also showed a strong ACEI activity with an IC50 value of 1.775 mg/mL. The components of the bioactive casein hydrolysate were analyzed by nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (NanoLC-Q-TOF-MS/MS). Total of 115 peptides were identified, among which 34, 9, 55 and 17 peptides were derived from α_(s1-), α_(s2-), β-, and κ-casein, respectively. The results of PeptideRanker and PepSite 2 analysis showed that 6 peptides (FRQFYQL, NENLLRF, NPWDQVKR, PVVVPPFLQ, PVRGPFPIIV, and ARHPHPHLSF) have both ACEI and anticoagulant activities by binding to the active sites of ACE and thrombin. This study indicated that casein is a potential functional food supplement that can be used for medical purposes.

关键词： Casein Anticoagulant ACE inhibitory Dual-function NanoLC-Q-TOF-MS/MS

Biogenic volatile organic compounds dominated the near-surface ozone generation in Sichuan Basin,China,during fall and wintertime

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Journal of Environmental Sciences 2024年第7期141卷 215-224页

作者： Dasheng Huang Qing Li Yan Han Shi-Yong Xia Jiawei Zhou hanxiong Che Keding Lu Fumo Yang Xin Long Yang Chen Research Center for Atmospheric Environment Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China Chongqing Institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714China College of Resources and Environment Chongqing SchoolUniversity of the Chinese Academy of Sciences(UCAS Chongqing)Chongqing 400714China College of Environmental and Chemical Engineering Chongqing Three Gorges UniversityChongqing 404199China Key Laboratory for Urban Habitat Environmental Science and Technology School of Environment and EnergyPeking University Shenzhen Graduate SchoolLishui RoadNanshan DistrictShenzhen 518055China SKL-ESPC and BIC-ESAT College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China College of Architecture&Environment Sichuan UniversityChengdu 610065China

The complex air pollution driven by both Ozone(O_(3))and fine particulate matter(PM2.5)sig nificantly influences the air quality in the Sichuan Basin(SCB).Understanding the O_(3)for mation during autumn and winter is necessary to understand the atmospheric oxidative ***,continuous in-site field observations were carried out during the late summer,early autumn and winter of 2020 in a rural area of *** total volatile organic compounds(VOCs)concentration reported by a Proton-Transfer-Reaction Time-of Flight Mass Spectrometry(PTR-ToF-MS)were 13.66±9.75 ppb,5.50±2.64 ppb,and 9.41±5.11 ppb in late summer,early autumn and winter,*** anthropogenic VOCs(AVOCs)and biogenic VOCs(BVOCs)were 8.48±7.92 ppb and 5.18±2.99 ppb in late sum mer,3.31±1.89 ppb and 2.19±0.93 ppb in autumn,and 6.22±3.99 ppb and 3.20±1.27ppb in winter.A zero-dimensional atmospheric box model was employed to investigate the sensitivity of O_(3)-precursors by relative incremental reactivity(RIR).The RIR values of AV OCs,BVOCs,carbon monoxide(CO),and nitrogen oxides(NOx)were 0.31,0.71,0.09,and-0.36 for late summer,0.24,0.59,0.22,and-0.38 for early autumn,and 0.30,0.64,0.33 and-0.70 for winter,and the results showed that the O_(3)formation of sampling area was in the VOC-limited region,and O_(3)was most sensitive to BVOCs(with highest RIR values,>0.6)This study can be helpful in understanding O_(3)formation and interpreting the secondary formation of aerosols in the winter.

关键词： VOCs PTR-MS Model Ozone

基于药效团模型筛选鳕鱼源可溶性环氧化物水解酶抑制肽及其作用机制

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食品科学 2023年第6期44卷 220-226页

作者：刘攀涂茂林刘汉雄程述震徐献兵杜明大连工业大学食品学院国家海洋食品工程技术研究中心辽宁大连116034 宁波大学食品与药学学院浙江宁波315800

以可溶性环氧化物水解酶(soluble epoxide hydrolase,sEH)为靶点的生理性抑制剂在治疗高血压、炎症、心血管疾病及糖尿病等方面具有显著的疗效。以大西洋真鳕鱼多肽粉为原料,构建Hypogen药效团,结合分子对接法筛选含有色氨酸且抑制sEH... 详细信息

以可溶性环氧化物水解酶(soluble epoxide hydrolase,sEH)为靶点的生理性抑制剂在治疗高血压、炎症、心血管疾病及糖尿病等方面具有显著的疗效。以大西洋真鳕鱼多肽粉为原料,构建Hypogen药效团,结合分子对接法筛选含有色氨酸且抑制sEH活性的生物活性肽,通过固相合成技术制备生物活性肽序列,并采用高效液相色谱法测定其sEH体外抑制活性。结果表明,利用最佳药效团模型(1号药效团)筛选出的四肽(PLLW)具备最优的Fit值(9.053)和LibDock评分(147.807),其半抑制浓度(IC_(50))为506.66μmol/L。分子对接结果表明,PLLW通过氢键和疏水相互作用与生理性底物竞争结合sEH活性位点,起到抑制活性。本研究为食源性混合体系中sEH抑制剂的筛选及机制研究提供了一种新思路,为sEH抑制剂产品的开发提供了一个合适的模型。

关键词：鳕鱼多肽可溶性环氧化物水解酶抑制剂分子对接

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周向定位旋转分度钻模设计

制造技术与机床 2017年第10期 55-57页

作者：娄高峰刘汉雄西北工业集团有限公司陕西西安710043

针对轴类工件两端圆周上分布有多孔且周向位置有要求一类零件的结构特点,在没有数控四轴钻铣床的条件下,研究了一种既能保证其精度和技术要求,又可以高效生产的加工工艺;设计、制造出了周向定位旋转分度的钻模,已用于实际生产当中,效果... 详细信息

针对轴类工件两端圆周上分布有多孔且周向位置有要求一类零件的结构特点,在没有数控四轴钻铣床的条件下,研究了一种既能保证其精度和技术要求,又可以高效生产的加工工艺;设计、制造出了周向定位旋转分度的钻模,已用于实际生产当中,效果良好。

关键词：钻模周向定位旋转分度轴向支承

基于Citespace的边坡离散元研究文献计量分析

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路基工程 2023年第1期 63-69页

作者：刘汉雄中交第二公路勘察设计研究院有限公司武汉430056

基于Web of science数据库,以2010~2021年检索的851篇边坡离散元期刊论文为样本,利用Citespace对该领域内论文发表数量、国家/地区和机构、作者和期刊以及高中心性关键词进行文献计量和可视化分析。结果表明:目前中国处于边坡离散元研... 详细信息

基于Web of science数据库,以2010~2021年检索的851篇边坡离散元期刊论文为样本,利用Citespace对该领域内论文发表数量、国家/地区和机构、作者和期刊以及高中心性关键词进行文献计量和可视化分析。结果表明:目前中国处于边坡离散元研究领先地位,国际合作较少,核心发文机构为中国科学院和中国地质大学;GEOTECHNIQUE和ENG GEOL杂志为边坡离散元研究的代表性期刊,涉及学科主要为工程技术和地学;自2010~2021年离散元在边坡方向研究的总体发展脉络清晰,前期以完善基础知识为主,后期则着重于结合工程实际。研究结果有助于理清边坡离散元的整体研究脉络,对研究现状和热点提供分析。

关键词：边坡离散元 Web of Science数据库 Citespace 可视化图谱

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褶皱山地高速公路桥梁优化设计

中国建材 2023年第2期 126-128页

作者：刘汉雄中交第二公路勘察设计研究院有限公司

我国山地、沟壑等占国土面积的69%,地势一般呈现西高东低的特征,总体地形特点是山高谷深,山势险峻,山沟迂回,陡崖峭壁,地质环境相对复杂,路线区内的不良地貌条件主要为塌方、斜坡、危岩体、石堆和岩溶等。高速公路桥梁设计与施工过程中... 详细信息

我国山地、沟壑等占国土面积的69%,地势一般呈现西高东低的特征,总体地形特点是山高谷深,山势险峻,山沟迂回,陡崖峭壁,地质环境相对复杂,路线区内的不良地貌条件主要为塌方、斜坡、危岩体、石堆和岩溶等。高速公路桥梁设计与施工过程中易受地形和地势的限制,增加了施工中的建设难度。

关键词：高速公路桥梁国土面积陡崖地貌条件地形特点危岩体山高谷深地质环境

微乳突提高低力学刺激下微结构柔性压力传感器的性能

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机械工程学报 2024年第4期60卷 222-229页

作者：赵家浩黄汉雄华南理工大学广东省高分子先进制造技术及装备重点实验室广州510640

提出一种新的快速制备双级微圆顶阵列柔性压力传感器的方案,并应用于检测微弱的生理信号。采用模压成型,快速制备表面分布有双级、单级微圆顶阵列和表面平整的热塑性聚氨酯柔性薄片;将喷金的微结构薄片和涂覆导电石墨的平整薄片作为传... 详细信息

提出一种新的快速制备双级微圆顶阵列柔性压力传感器的方案,并应用于检测微弱的生理信号。采用模压成型,快速制备表面分布有双级、单级微圆顶阵列和表面平整的热塑性聚氨酯柔性薄片;将喷金的微结构薄片和涂覆导电石墨的平整薄片作为传感基片,以面对面方式封装成柔性压阻式压力传感器。结果表明,与单级微结构传感器相比,微圆顶表面上分布有微乳突的双级微结构传感器(D-Sensor)在线性低压区(0~11kPa)具有较高的灵敏度(0.061kPa^(-1))和明显较高的线性度(线性相关判定系数达0.996)。这归因于在压力作用下双级微圆顶的形变遵循Archard理论,接触区面积随压力提高的增加量较大。D-Sensor的响应时间较短(100 ms),在1000次循环压缩/释放测试(9 kPa压力)中能保持较稳定的压阻响应。D-Sensor可准确地检测脉搏、发音和呼吸等生理信号(对应低力学刺激)。研究表明,所提出的快速方法制备的双级微结构传感器具有优良的传感性能,可应用于健康检测领域。

关键词：柔性压力传感器微圆顶阵列微形变热塑性聚氨酯模压成型

贻贝盐溶蛋白特性分析及其ACE抑制肽的酶法制备

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食品科学 2018年第22期39卷 51-56页

作者：乔美玲刘汉雄樊凤娇涂茂林于翠平杜明大连工业大学食品学院国家海洋食品工程技术研究中心辽宁大连116034 哈尔滨工业大学化工与化学学院黑龙江哈尔滨150001

以贻贝为原料提取盐溶性蛋白,首先研究盐溶性蛋白的分子质量分布、粒度分布和变性温度等性质,然后比较其3种蛋白酶(胰蛋白酶、中性蛋白酶和胰酶)酶解产物的血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制活性,筛选出ACE抑制... 详细信息

以贻贝为原料提取盐溶性蛋白,首先研究盐溶性蛋白的分子质量分布、粒度分布和变性温度等性质,然后比较其3种蛋白酶(胰蛋白酶、中性蛋白酶和胰酶)酶解产物的血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制活性,筛选出ACE抑制活性较高的胰蛋白酶酶解物,其IC_(50)值为215.96 μg/mL。利用质谱对胰蛋白酶酶解物中的多肽氨基酸序列进行鉴定,利用多肽序列与ACE的对接分数初步筛选出11个分值大于180分的多肽序列,通过氨基酸组成结合情况和两者间氢键等相互作用,进一步筛选活性肽并阐述抑制活性机理,最终推测出LYDIDVAK和WIAEEADK是活性较高的抑制肽。

关键词：贻贝盐溶性蛋白超高效液相色谱-四极杆-飞行时间串联质谱血管紧张素转化酶抑制肽分子对接