检索结果-南通市图书馆

Stability of fluorochrome based assays to measure subcellular sperm functions

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2008年第3期10卷 455-459页

作者： Sonja Grunewald Manja Rasch Martin reinhardt Thomas Baumann Uwe Paasch Hans-Juergen Glander Department of Dermatology/Andrology Unit European Academy of Andrology （EAA ） Training Center University of Leipzig Leipzig 04103 Germany

Aim： To evaluate the long-term stability of the fluorescence signals of new fluorescence-based semen analysis assays for clinical application. Methods： Semen samples from 87 unselected infertile patients were used to perform the following assays：（i） detection of active caspase-3 （n = 17）; （ii） integrity of the mitochondrial membrane potential （MMP）（n = 17）; （iii） externalization of phosphatidylserine （EPS）（n = 16）; and （iv） detection of intact acrosomes via CD46 （n = 37）. After the assays, 4% paraformaldehyde was added to all aliquots. The fluorescence intensity of each sample was evaluated by flow cytometry on days 0, 3, 7, 10 and 14. Results： Differences of up to ± 5% positive spermatozoa from the value measured at day 0 were estimated as acceptable deviation. The Caspase-3 FLICA^TM showed mean differences 〈 5% at day 3, 7 and 10. At day 14 the mean difference was 7.6%. In contrast, the disrupted MMP and the EPS detection showed differences 〉 5% at day 3. The CD46-FITC labeling displayed absolute differences 〈 5% CD46-positive spermatozoa at days 3, 7, 10 and 14. Conclusion： Although immediate analysis of the fluorescence signals is recommended, it is possible to evaluate caspase-3 activation up to 10 days and CD46 up to 14 days after staining of sperm. The FACS evaluation of MMP and EPS detection should be conducted on the same day.

关键词： human spermatozoa apoptosis assays caspase-3 mitochondrial membrane potential integrity CD46 semen analysis

An exotic Cretaceous kimberlite linked to metasomatized lithospheric mantle beneath the southwestern margin of the S?o Francisco Craton,Brazil

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Geoscience Frontiers 2022年第1期13卷 182-203页

作者： Luísa D.V.Carvalho Tiago Jalowitzki Ricardo Scholz Guilherme O.Gonçalves Marcelo Peres Rocha Rogério Silvestre Pereira Cristiano Lana Marco Paulo de Castro Gláucia Queiroga reinhardt A.Fuck Universidade Federal de Ouro Preto(UFOP) Escola de MinasDepartamento de GeologiaCampus Morro do Cruzeiro35400-000 Ouro PretoMGBrazil Universidade de Brasília(UnB) Instituto de GeociênciasCampus Universitário Darcy Ribeiro70910-900 BrasíliaDFBrazil Independent Geologist Consultant

We present major and trace element compositions of mineral concentrates comprising garnet xenocrysts,ilmenite,phlogopite,spinel,zircon,and uncommon minerals(titanite,calzirtite,anatase,baddeleyite and pyrochlore)of a newly discovered Late Cretaceous kimberlite(U-Pb zircon age 90.0±1.3 Ma;2σ)named Osvaldo Franca 1,located in the Alto Paranaíba Igneous Province(APIP),southeastern *** grains are lherzolitic(Lherz-1,Lherz-2 and Lherz-3),harzburgitic(Harz-3)and wehrlitic(Wehr-2).The pyrope xenocrysts cover a wide mantle column in the subcratonic lithosphere(66–143 km;20–43 kbar)at relatively low temperatures(811–875°C).The shallowest part of this mantle is represented by Lherz-1 pyropes(20–32 kbar),which have low-Cr(Cr_(2)O_(3)=1.74–6.89 wt.%)and fractionated middle to heavy rare earth elements(MREE-HREE)*** deepest samples are represented by Lherz-2,Lherz-3,Harz-3,and Wehr-2 pyropes(36–43 kbar).They contain high-Cr contents(Cr_(2)O_(3)=7.36–11.19 wt.%)and are characterized by sinusoidal(Lherz-2 and Wehr-2)and spoon-like(Lherz-3 and Harz-3)REE *** to their REE and trace elements,pyrope xenocrysts have enriched nature(e.g.,Ce and Yb ***_(2)O_(3)),indicating that the cratonic lithosphere has been affected by a silicate melt with subalkaline/tholeiite composition due to their low Zr,Ti and Y *** minerals with typical kimberlitic signatures,such as ilmenite and zircon,the exotic compositions of phlogopite and ulvöspinel suggest an enriched component in the magma *** formation of rare mineral phases with strong enrichment of light-REE(LREE)and high field strength elements(HFSE)is attributed to the late-stage kimberlitic *** propose a tectonic model where a thermal anomaly,represented by the low-velocity seismic anomaly observed in P-wave seismic tomography images,supplied heat to activate the alkaline magmatism from a metasomatized cratonic mantle source during the late-stages of Gondwana fragmentation and conse

关键词： Osvaldo França 1 kimberlite Alto Paranaíba Igneous Province Metasomatized cratonic lithosphere Zircon U-Pb geochronology

Evaluation of a generic set to measure functional health based on the International Classification of Functioning,Disability and Health in China’s mainland:a prospective cohort study

在线全文

学校读者我要写书评

暂无评论

Evaluation of a generic set to measure functional health bas...

中华医学会第十五次全国物理医学与康复学学术会议

作者： XiaZhang Jan D.reinhardt MelissaSelb JiananLi The First Affiliated Hospital of Nanjing Medical University Institute for Disaster Management and Reconstruction Sichuan UniversityChengdu Hong Kong Polytechnical University Swiss Paraplegic Research Department of Health Sciences and Health Policy University of Lucerne

＜正＞Background We piloted the implementation of a generic set to measure functional health based on the InternationalClassification of Functioning,Disability and Health across China’s *** aimed to evaluate 1)the feasibility of using the Generic ICF Set-based assessment tool（GISAT）in clinical practice,2)its sensitivity to change,and 3)its ability to predict length of stay.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

扭转荷载作用下混凝土的软化

河海大学学报（自然科学版） 1991年第1期19卷 31-39页

作者：徐道远 H.W.reinhardt 河海大学联邦德国Darmstadt工业大学

笔者通过对带环状切口的圆柱试样在扭转荷载作用下的试验,进行了混凝土软化性能的研究,并用控制位移法对普通混凝土和掺气混凝土Ⅲ型荷载-位移全曲线进行了测试,分别算得了其Ⅲ型断裂能G?.结果表明,G?不是材料常数,它随着试样尺寸的增... 详细信息

笔者通过对带环状切口的圆柱试样在扭转荷载作用下的试验,进行了混凝土软化性能的研究,并用控制位移法对普通混凝土和掺气混凝土Ⅲ型荷载-位移全曲线进行了测试,分别算得了其Ⅲ型断裂能G?.结果表明,G?不是材料常数,它随着试样尺寸的增大而增大,也随切口深度的加深而增大.本文根据试验记录曲线,拟合出了混凝土Ⅲ型软化曲线的数学表达式,从而为数值分析计算提供了方便.

关键词：混凝土扭转荷载软化曲线

探索加蓬港口绩效：一种基于供应链和运输物流效率的方法（以Owendo码头为例）

在线全文

学校读者我要写书评

暂无评论

探索加蓬港口绩效：一种基于供应链和运输物流效率的方法（以Owen...

作者： MINKO AKOUMA EYOUNE Cyrus reinhardt（许加名）大连海事大学

学位级别：硕士

加蓬拥有885公里的海岸线,是非洲大西洋最宽的海岸线之一;在本地区提供最宽的海上窗户之一,并且为加蓬港口和海上活动提供了有利的航海条件。由于丰富的动植物资源,加蓬具有对敏感,且需要保护的自然环境。本研究首先评估加蓬欧文多集装... 详细信息

加蓬拥有885公里的海岸线,是非洲大西洋最宽的海岸线之一;在本地区提供最宽的海上窗户之一,并且为加蓬港口和海上活动提供了有利的航海条件。由于丰富的动植物资源,加蓬具有对敏感,且需要保护的自然环境。本研究首先评估加蓬欧文多集装箱码头的现状,然后评估加蓬港口的未来发展,以提供有效的信息来帮助实施港口的未来建设项目。通过调查与预测加蓬港口在2020年至2027年期间的吞吐量发展趋势,为加蓬国民经济发展需求提供科学依据,并预测未来的集装箱吞吐量。通过Excel软件所得到的加蓬港口吞吐量(2020年-2027年)的预测结果表明,如果加蓬港口保持稳定的发展状态,加蓬的预期港口吞吐量可达到221 KTEU在2022年;五年之后,通过类似的稳定状态,其预期港口吞吐量可以达到246 KTEU。本研究还通过应用数据包络分析(DEA)方法分析欧文多集装箱码头的效率,并将其效率与西非和中非的其他三个港口的效率进行比较,从而获得欧文多集装箱码头在所考虑的非洲地区的当前定位。以窗口分析所得到的结果来看,考虑到港口规模(码头面积和长度)和港口吞吐量,可以发现欧文多港口(加蓬)在西非和中非地区是最小的港口之一;但是,在2010年至2019年期间,欧文多港口(加蓬)是该地区的效率最高的港口:其平均相对效率得分为94%。反而,在本研究中,考虑到港口规模(码头面积和长度)和港口吞吐量,黑角港口(刚果布)是西非和中非地区最大的港口;但是,在2010年至2019年期间,黑角港口(刚果布)在该地区却是效率方面排名第二的港口:其平均相对效率得分为84.47%。该结果对文献增加了知识点,因为大众总是认为小港口就有较小的效率。本研究所分析的加蓬港口,尤其是欧文多港口,的效率处于当前的情况。在实施新的投资和组织管理的过程后,欧文多港口将会获得更多机会来提高其效率,并且这过程能促进欧文多港口在国际航运平台上的竞争力。这不仅会增加在欧文多港口处的海上运输,还会使得欧文多港口面临更多的挑战,例如连接西非和中非地区的海运网络系统的建设以及国家之间的交通物流。

关键词： DEA 港口现状供应链欧文多集装箱码头运输物流效率

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

人物动画

计算机光盘软件与应用（COMPUTER ARTS数码艺术） 2008年第4期 98-99页

作者： Will reinhardt

本次教程我们将会教你如何用Toon Boom Studio来创建和动画你自己的动漫人物以及2D人物。你可以让草稿中的人物成为现实，并将他们添加到自己的网页或者是短片中。

关键词：动画制作网页动漫人物设计方案

Use of a Weight of Evidence Approach to Determine the Likelihood of Adverse Effects on Human Health from the Presence of Uranium Facilities in Port Hope, Ontario

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2011年第9期2卷 1149-1161页

作者： Julie Burtt Michael Ilin Rachel Lane Patsy Thompson Marcelle Phaneuf Pascale reinhardt 不详

The radium and uranium processing industry exists in Port Hope, Ontario, since 1932. Between 1932 and 1966, most of the waste material from these industries was deposited throughout the town. During these years, waste management practices did not prevent the spread of contamination. Several environmental and health studies have been conducted to assess the potential contamination effects in the Port Hope Community over the last 70 years. The current study used a weight of evidence approach to assess the types and levels of contaminants of concern in the environment, and the potential human exposure to these contaminants. Their toxicological and radio-toxicological properties were also assessed to determine their potential health effects. The results of these assessments were further compared to findings of earlier epidemiological studies of Port Hope residents and nuclear industry workers. The conclusions of this study indicated that: 1) Levels of exposure to radioactive and non-radioactive contaminants in Port Hope are below levels known to cause adverse health effects. 2) Epidemiological studies provide no evidence of health effects as a result of past and present activities of the Port Hope nuclear industries. 3) The environmental risk assessments and the epidemiological studies are consistent and support each other. 4) Port Hope’s findings are consistent with the results of over 40 epidemiological studies conducted elsewhere on populations living around similar facilities or exposed to similar environmental contaminants.

关键词： Uranium Radiation Cancer Environment

ICF通用组合在中国人群临床应用中的效度研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国康复医学杂志 2017年第9期32卷 994-999页

作者：刘珊张霞方蘅英 Jan D.reinhardt 刘守国励建安蔡文智南方医科大学深圳医院深圳518000 北京大学第三医院康复科 Institute for Disaster Management and Reconstruction Sichuan University Swiss Paraplegic Research 南京医科大学第一附属医院康复医学科

目的:探讨ICF通用组合在中国人群临床功能评估中的结构效度,为其临床应用提供依据。方法:采用ICF通用组合功能评估量表,于入院和出院时两个时间点对来自全国21个省份和自治区50家医院康复医学科、神经内科或脑外科、心血管科、呼吸科和... 详细信息

目的:探讨ICF通用组合在中国人群临床功能评估中的结构效度,为其临床应用提供依据。方法:采用ICF通用组合功能评估量表,于入院和出院时两个时间点对来自全国21个省份和自治区50家医院康复医学科、神经内科或脑外科、心血管科、呼吸科和骨科4510名患者进行评估。所得数据用于评价ICF通用组合在临床功能评估中的结构效度。结果:"d850有报酬的就业"不适用于NRS评分,缺失值较大。除去此项,患者出院时ICF通用组合评分(15.25±13.48)分,低于入院时(23.76±15.15)分,差异有显著性意义(P1的公因子共有2个,累积贡献率为78.018%。采用结构方程模型方法,拟合了"b130能量和驱力功能"、"b152情感功能"、"b280痛感"、"d230执行日常事务"、"d450步行"、"d455到处移动"关系模型,结构方程模型调整的拟合优度指数RMSEA=0.026,NFI=0.999,CFI=0.999,AGFI=0.994,模型较好的拟合了数据。结论:ICF通用组合能反映出、入院时患者的不同功能状态且其结构效度良好,是一种临床上有效的测量患者功能状态的工具。

关键词： ICF通用组合结构效度效度

在线全文

学校读者我要写书评

暂无评论

Flash 4宝典