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Sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells

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Chinese Medical Journal 2003年第12期116卷 1923-1929页

作者：赵进顺 Alexandra Schmid-Kotsas hans-juergen Gross Adolf Gruenert Max G. Bachem Department of Occupational and Environmental Medicine Department of Clinical Chemistry and Pathobiochemistry

Objective To study the sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent *** Sensitivity and specificity of several common methods for apoptosis determination were evaluated (Apo2.7-expression, annexin V-binding, TUNEL-reaction, poly-(ADP-ribose)-polymerase-(PARP) cleavage and single-stranded-DNA (ssDNA) staining). Apoptosis was induced by oxidative stress generated by hydrogen peroxide in 3 cultured cells types growing as adherent monolayer (MiaPaCa-2, Hep-G2 and human skin fibroblasts), necrosis was induced by depletion of cellular ATP using sodium azide. Cells positively stained by the respective apoptosis assay were quantified and alterations of cell morphology were monitored by fluorescence microscopy. The date was analyzed by one-way analysis of variance and significance test of correlation *** One hour after apoptosis induction significant cell fractions were positively stained for ssDNA (33% with MiaPaCa-2 cells, 35% with Hep-G2 cells, 56% with human skin fibroblasts). PARP-cleavage was less sensitive compared to the ssDNA-staining. Apo2.7-expression, annexin V-binding and TUNEL-reaction were not applicable to detect early apoptosis induced by oxidative stress (below 2 hours), but were efficiently monitoring late apoptosis. Specificity of ssDNA-staining was complete with each cell type even 4 hs after induction of necrosis by the highest sodium azide concentration. In contrast, the same experimental conditions resulted in 50%-90% positively stained necrotic cells by using Apo2.7-expression, TUNEL-reaction or annexinV-binding. Surprisingly, specificity of PARP-cleavage was highly depending on the respective cell *** Our study prove that among the five methods investigated only ssDNA-staining allowed to completely differentiate apoptosis from necrosis, and is thus suitable to reliably detect early as well as late apoptosis. Therefore, the ssDNA-stainin

关键词： apoptosis staining method sensitivity specificity oxidative stress adherent cell

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相图热力学在能源材料中的应用（英文）

中国材料进展 2015年第4期34卷 289-296,316页

作者： hans juergen Seifert 卡尔斯鲁厄理工学院应用材料物理研究所德国埃根施泰因莱奥波尔茨港76344

CALPHAD结合关键实验可以探究能源材料体系的多元多相反应。介绍了4个基于不同应用背景的合金体系的评估工作:液相烧结的Si C(LPSSi C)体系、转换型锂离子电池体系、掺杂Cr的Ti Al基合金体系以及Zr基热障涂层体系。研究结果显示:CALPHA... 详细信息

CALPHAD结合关键实验可以探究能源材料体系的多元多相反应。介绍了4个基于不同应用背景的合金体系的评估工作:液相烧结的Si C(LPSSi C)体系、转换型锂离子电池体系、掺杂Cr的Ti Al基合金体系以及Zr基热障涂层体系。研究结果显示:CALPHAD热力学计算结果可以用于指导关键实验设计,从而进一步明确研究体系的相图和热力学性质。获得的实验数据又可以作为精炼CALPHAD模型和改进模型参数的输入条件。精炼后的模型参数构成的热力学数据库可用于计算各种相图(包括等温截面、垂直截面、可以用来计算可信的相图(等温截面、垂直截面及波动图)及液相面投影图。

关键词：能源材料 CALPHAD LPSSiC 锂离子电池 TiAl基合金热障涂层

A New Silk Road between Asia ＆ Europe – the Conception of a Magic Triangle

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A New Silk Road between Asia ＆ Europe – the Conception of ...

Stability of fluorochrome based assays to measure subcellular sperm functions

第三届（2008）中国管理学年会——市场营销分会场

作者： hans-juergen Walz School of Economics and Management Tongji University

China′s Economy in History,Presence＆Future;China′s Comeback;Global Sourcing＆International Trade very important for today′s *** Trade Platforms strengthen the Exchange of *** Trade Platforms in three parts of the world（=Europe,Far East＆Middle East）,called a"Magic Trade＆Exhibition Triangle".Two European elements:Gate-to-Asia-Project in Duisburg,Germany-main aspects:permanent exhibition only for wholesalers＆adding Quality Assurance to the suppliers-and AsiaCenter,Budapest for Eastern *** Global Market Place in Tianjin,P.R.C.,is Far East counterpart:Unique combination of traditional exhibition＆permanent *** the way between China＆Europe:Dragon Complex in booming Dubai,Middle ***-Concept helps SME`*** is worldwide cooperation to generate wealth,trust,stability,respect,friendship＆peace.

关键词： China′s Economy China′s Comeback Sourcing International Trade Trade Platforms Quality-Concept

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Asian Journal of Andrology 2008年第3期10卷 455-459页

作者： Sonja Grunewald Manja Rasch Martin Reinhardt Thomas Baumann Uwe Paasch hans-juergen Glander Department of Dermatology/Andrology Unit European Academy of Andrology （EAA ） Training Center University of Leipzig Leipzig 04103 Germany

Aim： To evaluate the long-term stability of the fluorescence signals of new fluorescence-based semen analysis assays for clinical application. Methods： Semen samples from 87 unselected infertile patients were used to perform the following assays：（i） detection of active caspase-3 （n = 17）; （ii） integrity of the mitochondrial membrane potential （MMP）（n = 17）; （iii） externalization of phosphatidylserine （EPS）（n = 16）; and （iv） detection of intact acrosomes via CD46 （n = 37）. After the assays, 4% paraformaldehyde was added to all aliquots. The fluorescence intensity of each sample was evaluated by flow cytometry on days 0, 3, 7, 10 and 14. Results： Differences of up to ± 5% positive spermatozoa from the value measured at day 0 were estimated as acceptable deviation. The Caspase-3 FLICA^TM showed mean differences 〈 5% at day 3, 7 and 10. At day 14 the mean difference was 7.6%. In contrast, the disrupted MMP and the EPS detection showed differences 〉 5% at day 3. The CD46-FITC labeling displayed absolute differences 〈 5% CD46-positive spermatozoa at days 3, 7, 10 and 14. Conclusion： Although immediate analysis of the fluorescence signals is recommended, it is possible to evaluate caspase-3 activation up to 10 days and CD46 up to 14 days after staining of sperm. The FACS evaluation of MMP and EPS detection should be conducted on the same day.

关键词： human spermatozoa apoptosis assays caspase-3 mitochondrial membrane potential integrity CD46 semen analysis

A Wide Range Aerosol Spectrometer for Environmental Monitoring of Airborne Particles from 5.5nm to 32μm

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A Wide Range Aerosol Spectrometer for Environmental Monitori...

Role of soluble factors and three-dimensional culture in in vitro differentiation of intestinal macrophages

2010年海峡两岸环境与能源研讨会

作者： juergen Spielvogel Markus Pesch hans Grimm GRIMM Aerosol Technik GmbH & Co.KG Dorfstrasse 9 D-83404 AinringBayernGermany

Ambient Airborne Particles are a varied and changing mixture of natural particles(e.g. sea-salt aerosols,dust),man-made particles(*** exhaust particles) and particles formed by gas reactions within the atmosphere(*** nitrate).Their size ranges from a few nanometers to tens of *** recent scientific and epidemiological studies found a significant relationship between ambient airborne particles and the respiratory and cardiovascular *** human health effects,ambient airborne particles also have a large effect on the climate *** factors have attracted many people’s interest in the environmental monitoring of airborne particles.

关键词： Airborne particles Environmental monitoring Particle size distribution Wide range aerosol spectrometer

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World Journal of Gastroenterology 2007年第7期13卷 1032-1041页

作者： Tanja Spoettl Martin Hausmann Katrin Menzel Heidi Piberger hans Herfarth juergen Schoelmerich Frauke Bataille Gerhard Rogler Department of Internal Medicine I and Institute of Pathology University of Regensburg Regensburg 93042 Germany

AIM: To examine the factor(s) involved in differentiation of intestinal macrophages (IMACs) using a recently established in vitro model. METHODS: To test whether soluble or membrane bound factors induce IMAC-differentiation, freshly elutriated monocytes (MO) were incubated with conditioned media or cell membranes of intestinal epithelial cells (IEC) or cultured with IEC in transwell systems. To determine the importance of an active migration of MO, three- dimensional aggregates from a 1:1-mixture of MO and IEC were examined by immunohistochemistry and flow cytometry. Apoptosis was examined by caspase-3 Western blots. Extracellular matrix production in differentiation models was compared by immunohistochemistry. RESULTS: IMAC differentiation was observed in a complex three-dimensional co-culture model (multicellular spheroid, MCS) with IEC after migration of MO into the spheroids. By co-culture of MO with conditioned media or membrane preparations of IEC no IMAC differentiation was induced. Co-culture of MO with IEC in transwell- cultures, with the two cell populations separated by a membrane also did not result in intestinal-like differentiation of MO. In contrast to IEC-spheroids with immigrating MO in mixed MCS of IEC and MO only a small subpopulation of MO was able to survive the seven day culture period. CONCLUSION: Intestinal-like differentiation of MO in vitro is only induced in the complex three-dimensional MCS model after immigration of MO indicating a roleof cell-matrix and/or cell-cell interactions during the differentiation of IMACs.

关键词： Intestinal macrophages Intestinal epithelialcells Multicellular spheroids Inflammatory boweldisease Tolerance differentiation

中国初始飞行学员心理测量选拔方法的发展和效度

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中华航空航天医学杂志 2002年第2期13卷 102-102页

作者： hans-juergen hoermann huijie guan 德国宇航中心航空航天心理学部德国汉堡 Christian-Albrechts大学心理学系德国Kiel

关键词：飞行员选拔心理测试测验效度中国

SupraBiology 2014： Promoting UK-China collaboration on Systems Biology and High Performance Computing

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Frontiers of Electrical and Electronic Engineering in China 2015年第1期10卷 46-53页

作者： Ettore Murabito Riccardo Colombo Chengkun Wu Malkhey Verma Samrina Rehman Jacky Snoep Shao-Liang Peng Naiyang guan Xiangke Liao hans V. Westerhoff Manchester Institute of Biotechnology School of Computer Science Faculty of Engineering and Physical Sciences Manchester Centre for Integrative Systems Biology The University of Manchester Manchester M139PL United Kingdom. Department of Informatics Systems and Communication University of Milan-Bicocca Milan 20126 Italy. SYSBIO - Centre of Systems Biology Milan 20126 Italy. Manchester Institute of Biotechnology School of Chemical Engineering and Analytical Sciences Manchester Centre forIntegrative Systems Biology The University of Manchester Manchester M139PL United Kingdom. School of Computer Science National University of Defence Technology Changsha 410073China

In the second half of 2014 the Manchester Institute of Biotechnology, based in Manchester （UK）, hosted the first SupraBiology congress, an event attended by representa- tives of different academic institutions and industry based in both the UK and China. The congress was aimed to serve as a platform to discuss and promote potential collaborations between the UK and China on the subject of Systems Biology and High Performance Computing. The event, sponsored by the ＂BBSRC China Partnering Awards＂ and ISBE, was organised as a sequence of talks addressing the different aspects of Systems Biology that can benefit from High Performance Computing. A general discussion session followed where the scientific, techni- cal, and logistic aspects of the prospected UK-China collaborations were examined.

关键词： 2014 UK-China High Performance Computing

多层螺旋CT对先天性右冠状动脉起始变异的评价

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中华放射学杂志 2006年第3期40卷 277-280页

作者：史河水韩萍孔祥泉冯敢生 hans-juergen Brambs Martin H.K.Hoffmann 华中科技大学同济医学院附属协和医院放射科武汉430022 Department of Diagnostic Radiology University Hospital of Ulm

目的以常规X线冠状动脉造影(CAG)为标准,评价16层螺旋CT在鉴别右冠状动脉(RCA)异常起源和异常行程中的价值。方法回顾性分析8例先天性RCA变异患者的多层螺旋CT造影(MSCTA)表现,其中7例有CAG资料。对比分析两者在显示RCA异位开口和异常... 详细信息

目的以常规X线冠状动脉造影(CAG)为标准,评价16层螺旋CT在鉴别右冠状动脉(RCA)异常起源和异常行程中的价值。方法回顾性分析8例先天性RCA变异患者的多层螺旋CT造影(MSCTA)表现,其中7例有CAG资料。对比分析两者在显示RCA异位开口和异常行程方面的差异。仿真内镜技术用于评价异位开口及其与邻近正常冠状动脉开口的关系。采用多平面重组(MPR)、曲面多平面重组(CMPR)、最大密度投影(MIP)、容积成像(VR)重组方法评价冠状动脉的异常行径及其与邻近大血管的关系。CAG所显示的RCA,至少有2个不同的视角用于评价其起源和行程。结果8例患者变异的RCA全部为MSCTA所显示。其中6例患者的RCA起源于左冠状动脉窦,1例起源于左主冠状动脉的末端,另1例则起源于扩大前移的后冠窦。所有异常开口均未见狭窄。8支异常冠状动脉均穿过主动脉根部和肺动脉或右室流出道的间隙。7例CAG则仅5例显示异常。结论MSCTA显示先天性变异的RCA明显优于CAG,凡疑为冠状动脉变异的患者,可首选非创伤性的16层MSCTA检查。

关键词：体层摄影术,X线计算机冠状血管造影术冠状血管畸形