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Flexural behavior of high-strength,steel-reinforced,and prestressed concrete beams

Frontiers of Structural and Civil Engineering 2021年第1期15卷 227-243页

作者： Qing JIANG Hanqin WANG Xun CHONG Yulong FENG Xianguo YE School of Civil and Hydraulic Engineering Hefei University of TechnologyHefei 230009China Anhui Key Laboratory of Civil Engineering and Materials Hefei 230009China College of Water Conservancy and Civil Engineering Xinjiang Agricultural UniversityUrumqi 830052China

To study the flexural behavior of prestressed concrete beams with high-strength steel reinforcement and high-strength concrete and improve the crack width calculation method for flexural components with such reinforcement and concrete,12 specimens were tested under static loading.The failure modes,flexural strength,ductility,and crack width of the specimens were analyzed.The results show that the failure mode of the test beams was similar to that of the beams with normal reinforced concrete.A brittle failure did not occur in the specimens.To further understand the working mechanism,the results of other experimental studies were collected and discussed.The results show that the normalized reinforcement ratio has a greater effect on the ductility than the concrete strength.The cracking-and peak-moment formulas in the code for the design of concrete(GB 50010-2010)applied to the beams were both found to be acceptable.However,the calculation results of the maximum crack width following GB 50010-2010 and EN 1992-1-1:2004 were considerably conservative.In the context of GB 50010-2010,a revised formula for the crack width is proposed with modifications to two major factors:the average crack spacing and an amplification coefficient of the maximum crack width to the average spacing.The mean value of the ratio of the maximum crack width among the 12 test results and the relative calculation results from the revised formula is 1.017,which is better than the calculation result from GB 50010-2010.Therefore,the new formula calculates the crack width more accurately in high-strength concrete and high-strength steel reinforcement members.Finally,finite element models were established using ADINA software and validated based on the test results.This study provides an important reference for the development of high-strength concrete and highstrength steel reinforcement structures.

关键词： high-strength steel reinforcement high-strength concrete flexural behavior crack width

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Linking microbial community composition to farming pattern in selenium-enriched region:Potential role of microorganisms on Se geochemistry

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Journal of Environmental Sciences 2022年第2期34卷 269-279页

作者： Yanhong wang Xinyan Shi Xianxin Huang Chunlei Huang Helin wang hanqin Yin Yixian Shao Ping Li State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430074China Zhejiang Institute of Geological Survey Hangzhou 311203China

Selenium(Se)is an essential micronutrient for lives.Indigenous microbial communities play an important role on Se geochemistry in soils.In this study,the microbial community composition and functions of 53 soil samples were investigated using high-throughput sequencing.Samples were divided into 3 groups with different farming types based on the measured geochemical parameters and microbial functional structures.Results indicated that putative Se related bacteria Bacillus,Dyella,Paenibacillus,Burkholderia and Brevibacillus were dominant in dryland plantation soils which were characterized with higher available Se and low contents of H_(2)O,total organic carbon(TOC),NH_(4)^(+) and NO_(2)^(-).In contrast,the putative denitrifier Pseudomonas dominated in flooded paddy soils with higher TOC,NO_(3)^(-) and organic Se,whereas genera Rhizobium,Nitrosospira,and Geobacter preferred woodland soils with higher oxidation-reduction potential(ORP),pH,NH_(4)^(+) and Fe.Farming patterns resulted in distinct geochemical parameters including moisture,pH,ORP,TOC,and contents of soluble Fe,NO_(2)^(-) and NH_(4)^(+),shaping the microbial communities,which in turn affected Se forms in soils.This study provides a valuable insight into understanding of Se biogeochemistry in soils and prospective strategy for Se-rich agriculture production.

关键词： Selenium biogeochemistry Indigenous microbial community Farming patterns High-throughput sequencing FAPROTAX

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An effective process for the separation of U(Ⅵ),Th(Ⅳ)from rare earth elements by using ionic liquid Cyphos IL 104

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Chinese Chemical Letters 2022年第7期33卷 3422-3428页

作者： Jiawei Lu Kebao He Yi wang Geng Chen hanqin Weng Mingzhang Lin School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China

Separation and recovery of U(Ⅵ)and Th(Ⅳ)from rare earth minerals is a very challenging work in rare earth industrial production.In the present study,a homemade membrane emulsification circulation(MEC)extractor was used to separate U(Ⅵ)and Th(Ⅳ)from rare earth elements by using Cyphos IL 104 as an extractant.Batch experiments were carried out using a constant temperature oscillator to investigate the extraction parameters of the single element and the results indicated that Cyphos IL 104could reach the extraction equilibrium within 30 min for all the three elements,i.e.,U(Ⅵ),Th(Ⅳ),and Eu(Ⅲ).Besides,the MEC extractor possessed a strong phase separation ability.The extraction efficiencies of U(Ⅵ),Th(Ⅳ),La(Ⅲ),Eu(Ⅲ)and Yb(Ⅲ)increased with the increase of pH.La(Ⅲ),Eu(Ⅲ)and Yb(Ⅲ)were hardly extracted when pH≤1.50,which was beneficial for effectively separating U(Ⅵ)and Th(Ⅳ)from La(Ⅲ),Eu(Ⅲ)and Yb(Ⅲ).In the multi-stages stripping experiments,when the stripping stage number was 3,the effective separation could be achieved by using HCl and H_(2)SO_(4),since the stripping efficiency reached 80.0%and 100.0%for Th(Ⅳ)and U(Ⅵ),respectively.Slope method and FT-IR spectra showed that Cyphos IL 104 reacted with U(Ⅵ)and Th(Ⅳ)by chelation mechanism.The extraction of multi-elements indicated that U(Ⅵ)and Th(Ⅳ)could be well separated from the solution which contains all rare earth elements,and the extraction efficiencies of U(Ⅵ)and Th(Ⅳ)both were close to 100.0%.Based on the above experimental results,a flowchart for efficient separation of U(Ⅵ)and Th(Ⅳ)from rare earth elements was proposed.

关键词： Rare earth elements Actinides Solvent extraction Membrane emulsification Ionic liquid

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3-N-butylphthalide improves neuronal morphology after chronic cerebral ischemia

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Neural Regeneration Research 2014年第7期9卷 719-726页

作者： Wanhong Zhao Chao Luo Jue wang Jian Gong Bin Li Yingxia Gong Jun wang Hanqin WANG Institute of Basic Medical Sciences Hubei University of Medicine

3-N-butylphthalide is an ettectwe drug for acute iscemlc stroke. However, its effects on cnromc cerebral ischemia-induced neuronal injury remain poorly understood. Therefore, this study li- gated bilateral carotid arteries in 15-month-old rats to simulate chronic cerebral ischemia in aged humans. Aged rats were then intragastrically administered 3-n-butylphthalide. 3-N-butylphtha- lide administration improved the neuronal morphology in the cerebral cortex and hippocampus of rats with chronic cerebral ischemia, increased choline acetyltransferase activity, and decreased malondialdehyde and amyloid beta levels, and greatly improved cognitive function. These findings suggest that 3-n-butylphthalide alleviates oxidative stress caused by chronic cerebral ischemia, improves cholinergic function, and inhibits amyloid beta accumulation, thereby im- proving cerebral neuronal injury and cognitive deficits.

关键词： nerve regeneration depression functional MRI graph theory complex networks brainnetwork classification feature selection NSFC grant neural regeneration

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Pim1 kinase is crucial for early activation of the nitric oxide synthase(eNOS)and NO production by fluid shear stress

Pim1 kinase is crucial for early activation of the nitric ox...

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第十二届全国生物力学学术会议暨第十四届全国生物流变学学...

作者： Hanqin WANG Yu Sun Yanlin Zhang Center for Translational Medicine Suizhou HospitalHubei University of Medicine

Objective Pim1 kinase is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Recent evidence suggests that Pim1 kinase might be a critical regulator implicated endothelial activation and promote angiogenesis. Nitric oxide (NO), synthesized by eNOS, is an important regulator of cardiovascular homeostasis in response to fluid shear stress. The purpose of the present study was to determine

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A novel selenite-reducing bacterium Bacillus pseudomycoides SA14 isolated from Se-enriched soil and its potential Se biofortification on Brassica chinensis L.

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Journal of Earth Science 2022年

作者： Xianxin Huang Yanhong wang Helin wang Xinyan Shi Chunlei Huang hanqin Yin Yixian Shao Ping Li State Key Laboratory of Biogeology and Environmental Geology， China University of Geosciences Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science， China University of Geosciences Zhejiang Institute of Geological Survey

Microbial participation in biofortification can improve the availability of selenium (Se) in soil and contribute to the enrichment of Se in crops. In this study， a selenite (Se(Ⅳ)) reducing strain was isolated from Se-rich soil， and its Se transformation and bioenhancement ability were studied. The strain was identified as Bacillus pseudomycoides and could reduce more than 93.48% of 1.0 mmol/L Se(Ⅳ) in 54 h. The results of scanning electron microscope (SEM) and energy dispersive X-ray spectrometry (EDS) showed that Se(Ⅳ) was reduced to Se(0)， and Se nanoparticles (SeNPs) were eventually formed. In pot experiments， B. pseudomycoides SA14 could promote the bioavailable Se in soils and the concentration of Se in Brassica chinensis L.. The concentrations of water-soluble Se， ion exchange Se and carbonate-binding Se in soil were increased by 23.13%， 22.05% and 30.89%， respectively. The Se concentration of Brassica chinensis L. in pot experiments was increased by 145.05%. The relative abundance of Bacillus in soil increased from 0.97% to 2.08% in the pot experiments. As far as we know， this is the first report of Se reduction by B. pseudomycoides. This study might provide a prospective strategy for microbial fortification of Se in crops.

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Impact of Phosphate Solubilizing Bacterial Agent on Cadmium Bioavailability and Microbial Communities in Soil and Cd Accumulation in Lettuce Plants

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Journal of Earth Science 2023年

作者： Yue Yu Ping Li Yanhong wang Xinzhe Lu Chunlei Huang Songle Zhang hanqin Yin Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science China University of Geosciences Zhejiang Institute of Geosciences State Key Laboratory of Biogeology and Environmental Geology

Cadmium(Cd) contamination in soil can lead to food chain accumulation and greatly impacts on human health. Bioremediation has gained more and more attention due to its environmentfriendly, high efficiency and low-cost. In this work, we studied the impact of phosphate solubilizing bacterial agent(PSBA) on Cd bioavailability, microbial communities in soil and Cd accumulation in lettuce plants with pot experiment and field trial. Results of pot experiment showed that PSBA could decrease the bioavailability of Cd(Cd-acid extractable from 3.30 to 2.34 mg/kg, Cd-reducible from 1.94to 1.56 mg/kg), promote lettuce plants growth(increased with 33.85% height and with 33.65% fresh weight) and reduce the accumulation of Cd(from 5.85 to 3.73 mg/kg) in lettuce plants. High-throughput sequencing identified that PSBA could change the composition and structure of the soil microbial communities. The relative abundances of the three ecologically beneficial bacterial families of Pseudomonadaceae, Burkholderiaceae, and Enterobacteriaceae increased from 2.29% to 5.13%, 0.56% to 5.24%, and 1.87% to 16.93%, respectively. And the former two were positively correlated with redox potential(Eh)(R2: 0.474, p<0.05, R2: 0.590, p<0.01, respectively). The bacterial networks were more complex in PSBA treatment, reflecting through more links(from 1 893 to 2 185) and a higher average degree(from 38.242 to 45.052) and density(from 0.390 to 0.469). Results of field trial demonstrated that PSBA could also decrease Cd content in lettuce plants and microbial composition in soil. This study indicated that PSBA could be served as an alternative material in bioremediation of Cd contamination in soil.

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MicroRNA-29a modulates axon branching by targeting doublecortin in primary neurons

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Protein & Cell 2014年第2期5卷 160-169页

作者： hanqin Li Susu Mao Haitao wang Ke Zen Chenyu Zhang Liang Li Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology Nanjing University School of Life Sciences Nanjing 210093 China Department of Pharmacy 306th Hospital of PLA Beijing 100101 China

MicroRNAs （miRNAs） are endogenously expressed small, non-coding transcripts that regulate protein expression. Substantial evidences suggest that miRNAs are enriched in central nervous system, where they are hypothesized to play pivotal roles during neural devel- opment. In the present study, we analyzed miRNAs expression in mice cerebral cortex and hippocampus at different developmental stages and found miR-29a increased dramatically at postnatal stages. In addition, we provided strong evidences that miR-29a is enriched in mature neurons both in vitro and in v/vo. Further investigation demonstrated that the activation of gluta- mate receptors induced endogenous miR-29a level in primary neurons. Moreover, we showed that miR-29a directly regulated its target protein Doublecortin （DCX） expression, which further modulated axon branching in primary culture. Together, our results suggested that miR-29a play an important role in neuronal development of mice cerebrum.

关键词： MiRNAs are approximately 21 nucleotide non-codingtranscripts that are derived from hairpin precursors which

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Climate change induced eutrophication of cold-water lake in an ecologically fragile nature reserve

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Journal of Environmental Sciences 2019年第1期31卷 359-369页

作者： Xiaotian Lu Yonglong Lu Deliang Chen Chao Su Shuai Song Tieyu wang hanqin Tian Ruoyu Liang Meng Zhang Kifayatullah Khan State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences Department of Earth Sciences Goteborg University International Center for Climate and Global Change Research School of Forestry and Wildlife Sciences Auburn University Department of Environmental and Conservation Sciences University of Swat

Aquatic ecosystem sustainability around the globe is facing crucial challenges because of increasing anthropogenic and natural disturbances. In this study, the Tianchi Lake, a typical cold-water lake and a UNESCO/MAB(Man and Biosphere) nature reserve located in high latitude and elevation with the relatively low intensity of human activity was chosen as a system to examine the linkages between climate change and eutrophication. As a part of the UNESCO Bogda Man and Biosphere Reserve, Tianchi Lake has been well preserved for prevention from human intervention, but why has it been infected with eutrophication recent years? Our results show that climate change played a significant role in the eutrophication in the Tianchi Lake. Increased temperature, changed precipitation pattern and wind-induced hydrodynamic fluctuations in the summer season were suggested to make a major contribution to the accelerated eutrophication. The results also showed that the local temperature and precipitation changes were closely linked to the large-scale atmospheric circulation, which opens the door for the method to be applied in other regions without local climatic information. This study suggests that there is an urgent need to take into consideration of climate change adaptation into the conservation and management of cold-water lakes globally.

关键词： Eutrophication Climate change Atmospheric circulation Alpine lakes

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中学生家长教育焦虑问卷编制及质量验证

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心理学探新 2023年第4期43卷 362-368页

作者：万小芬王茜琛王汉秦康春花浙江师范大学心理学院浙江省儿童青少年心理健康与危机干预智能实验室金华321004

编制中学生家长教育焦虑问卷并对其质量进行验证。通过梳理已有研究,建构教育焦虑的五维结构,包含学业焦虑、成就焦虑、健康焦虑、沟通焦虑、人际焦虑。选取三批样本(n=200、n=673、n=584)进行施测,对所得数据的项目分析、信度分析和效... 详细信息

编制中学生家长教育焦虑问卷并对其质量进行验证。通过梳理已有研究,建构教育焦虑的五维结构,包含学业焦虑、成就焦虑、健康焦虑、沟通焦虑、人际焦虑。选取三批样本(n=200、n=673、n=584)进行施测,对所得数据的项目分析、信度分析和效度分析结果表明,中学生家长教育焦虑问卷具有良好的项目效度、结构效度、内部一致性信度和效标关联效度,可作为中学生家长教育焦虑评估工具。

关键词：家长教育焦虑中学生信度效度

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