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Genetic structure of Aedes albopictus(Diptera:Culicidae)populations in China and relationship with the knockdown resistance mutations

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Infectious Diseases of Poverty 2023年第3期12卷 31-40页

作者： Wenqi Shan Hao Yuan Hanming Chen Haowei Dong Qiuming Zhou Feng Tao Jie Bai Huiying chen Yajun Ma Heng Peng Department of Naval Medicine Naval Medical UniversityShanghai 200433China Department of Medical Microbiology and Parasitology College of Basic Medical SciencesNaval Medical UniversityShanghai 200433China

Background Mosquito control is needed to prevent dengue fever, which is mainly spread byAedes albopictus in China. Application of insecticides is one of the main mosquito control methods;however, this approach can fail due to the knockdown resistance (kdr) gene mutation that causes decreased sensitivity to insecticides inAe. albopictus. Thekdr mutation patterns among different regions in China differ significantly. However, the underlying mechanism and factors that influencekdr mutation remain unclear. To explore the potential influence of genetic background on the development of insecticide resistance inAe. albopictus, we analyzed the genetic structure ofAe. albopictus populations in China and its correlation with majorkdr mutations.Methods We collectedAe. albopictus from 17 sites in 11 provinces (municipalities) across China from 2016 to 2021 and extracted the genomic DNA from individual adult mosquitoes. We selected eight microsatellite loci for genotyping, and based on microsatellite scores, we estimated intraspecific genetic diversity, population structure, and effective population size. The association between the intrapopulation genetic variation and F1534 mutation rate was evaluated by the Pearson correlation coefficient.Results Based on variation analysis of the microsatellite loci of 453 mosquitoes representing 17 populations throughout China, more than 90% of the variation occurred within individuals, whereas only about 9% of the variation occurred among populations, indicating that field populations ofAe. albopictus are highly polymorphic. The northern populations tended to belong to gene pool I (BJFT 60.4%, SXXA 58.4%, SDJN 56.1%, SXYC 46.8%), the eastern populations tended to belong to pool III (SH 49.5%, JZHZ 48.1%), and the southern populations tended to belong to three different gene pools. Moreover, we observed that the greater the fixation index (FST), the lower the wild-type frequency of F1534 ofVSGC.Conclusions The degree of genetic differentiation amongAe. albopictus populations in China was

关键词： Aedes albopictus Population structure Microsatellite Knockdown resistance China

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基于网络药理学探讨郁金散治疗猪肠道炎症的作用机制

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中国兽医杂志 2024年第6期60卷 124-133页

作者：陈汉明李桦黎增权广州医科大学附属第二医院实验动物中心广东广州510260

为了利用网络药理学探讨郁金散治疗猪肠道炎症的作用靶点,并对靶点涉及的生物学过程和作用通路进行阐述,本试验借助中药系统药理学数据库(TCMSP)分别筛选郁金散的活性成分和靶点,利用美国国家生物技术信息中心(NCBI)、在线人类孟德尔遗... 详细信息

为了利用网络药理学探讨郁金散治疗猪肠道炎症的作用靶点,并对靶点涉及的生物学过程和作用通路进行阐述,本试验借助中药系统药理学数据库(TCMSP)分别筛选郁金散的活性成分和靶点,利用美国国家生物技术信息中心(NCBI)、在线人类孟德尔遗传(OMIM)和GeneCards数据库检索肠道炎症靶点,通过Uniprot数据库对靶点进行校正。利用Cytoscape 3.9.1软件和String网站分别构建共有靶点网络和核心靶点相互作用网络,利用用于注释、可视化和集成发现的数据库(DAVID)对靶点涉及的生物学过程和信号通路进行分析。结果显示:经选择物种“Sus scrofa”校正后,郁金散的活性成分有66个,靶点81个,猪肠道炎症靶点198个,郁金散治疗猪肠道炎症的预测靶点有38个,其中核心靶点25个,包括肿瘤坏死因子(TNF)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)等。基因本体论(GO)功能富集分析得到生物学过程(BP)条目91个,细胞组成(CC)条目3个,分子功能(MF)条目10个,其中生物学过程主要涉及免疫反应、RNA聚合酶II启动子的转录正调控和细胞对脂多糖的反应等;细胞组成主要涉及胞外区、细胞外间隙和白细胞介素-6受体复合体;分子功能主要涉及细胞因子活性、生长因子活性和白细胞介素-2受体结合等。京都基因与基因组百科全书(KEGG)通路富集分析得到83条通路,相关性较强的通路依次是癌症通路、南美锥虫病和细胞因子-细胞因子受体相互作用等。结果表明,郁金散通过调控TNF、IL-6和IL-10等靶点,参与癌症通路、南美锥虫病和细胞因子-细胞因子受体相互作用等通路,对猪肠道炎症发挥治疗作用。

关键词：郁金散猪肠道炎症网络药理学

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基于局部存储策略的声波全波形反演方法

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地球物理学报 2024年第6期67卷 2388-2401页

作者：杨瀚张明坤孙鹏远李红辉陈汉明周辉油气资源与工程全国重点实验室 CNPC物探重点实验室中国石油大学(北京)北京102249 油气勘探计算机软件国家工程研究中心西城区新康街5号东方地球物理公司北京100088

全波形反演作为一种高精度地下速度模型建模方法,自提出以来就受到了地震勘探领域的广泛关注.然而在时间域全波形反演中梯度计算需要存储或恢复震源波场,这会导致存储大量数据或额外的震源波场重构计算,使得全波形反演的实用化受到波场... 详细信息

全波形反演作为一种高精度地下速度模型建模方法,自提出以来就受到了地震勘探领域的广泛关注.然而在时间域全波形反演中梯度计算需要存储或恢复震源波场,这会导致存储大量数据或额外的震源波场重构计算,使得全波形反演的实用化受到波场重构的制约.为此,本文提出了一种基于局部存储策略的声波全波形反演方法.依据奈奎斯特采样定理设置存储间隔,通过存储每个空间网格点有限时间区间内的有效波场来计算梯度,达到减少存储震源波场数据量、避免波场重构进而提升效率的目的.与其他方法相比,本方法无需波场重构且所需内存大小与正演模拟的差分精度和记录时长均无关.二维和三维推覆体模型合成数据的反演测试均表明:本方法能够在不重构波场的情况下保证反演结果的精度和信噪比,有效提高计算效率并显著降低对计算机内存的需求.

关键词：局部存储策略全波形反演速度模型建模奈奎斯特采样提高计算效率

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Parametric Study of Operating Conditions on Performances of a Solid Oxide Electrolysis Cell

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Journal of Thermal Science 2023年第6期32卷 1973-1988页

作者： chen hanming WANG Jingyi XU Xinhai School of Mechanical Engineering and Automation Harbin Institute of TechnologyShenzhen518055China School of Science Harbin Institute of TechnologyShenzhen518055China

The operating conditions greatly affect the electrolysis performance and temperature distribution of solid oxide electrolysis cells(SOECs).However,the temperature distribution in a cell is hard to determine by experiments due to the limitations of in-situ measurement methods.In this study,an electrochemical-flow-thermal coupling numerical cell model is established and verified by both current-voltage curves and electrochemical impedance spectroscopy(EIS)results.The electrolysis performance and temperature distribution under different working conditions are numerically analyzed,including operating temperature,steam and hydrogen partial pressures in the fuel gas,inlet flow rate and inlet temperature of fuel gas.The results show that the electrolysis performance improves with increasing operating temperature.Increasing steam partial pressure improves electrolysis performance and temperature distribution uniformity,but decreases steam conversion rate.An inappropriately low hydrogen partial pressure reduces the diffusion ability of fuel gas mixture and increases concentration impedance.Although increasing the flow rate of fuel gas improves electrolysis performance,it also reduces temperature distribution uniformity.A lower airflow rate benefits temperature distribution uniformity.The inlet temperature of fuel gas has little influence on electrolysis performance.In order to obtain a more uniform temperature distribution,it is more important to preheat the air than the fuel gas.

关键词： solid oxide electrolysis cell electrolysis performance temperature distribution operating conditions EIS(electrochemical impedance spectroscopy)

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双竖杆空腹桁架的刚度及经济性分析

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山东理工大学学报（自然科学版） 2024年第1期38卷 14-21页

作者：陈顺云肖建春沈睿麟张翰铭盛夏邓懋贵州大学空间结构研究中心贵州贵阳550025 贵州省结构工程重点实验室贵州贵阳550025

为了满足空腹桁架实际工程需要,改善空腹桁架结构刚度及经济性,提出了一种双竖杆空腹桁架结构。推导了双竖杆空腹桁架等效惯性矩和等效剪切刚度的计算公式,通过刚度分析确定了双竖杆间距的合理取值。通过有限元参数化分析,验证了等效刚... 详细信息

为了满足空腹桁架实际工程需要,改善空腹桁架结构刚度及经济性,提出了一种双竖杆空腹桁架结构。推导了双竖杆空腹桁架等效惯性矩和等效剪切刚度的计算公式,通过刚度分析确定了双竖杆间距的合理取值。通过有限元参数化分析,验证了等效刚度计算公式的可靠性。在用钢量相同的条件下与传统空腹桁架进行静力性能对比分析,结果表明:双竖杆空腹桁架的等效剪切刚度提高0%~40%,上下弦弯矩和剪力分别减小35.3%和7.2%;腹杆弯矩和剪力减小39%。采用基于响应面法的结构优化设计方法对传统空腹桁架与双竖杆空腹桁架进行优化后对比分析,结果表明:在挠度、最大应力和型钢宽厚比满足规范的条件下,双竖杆空腹桁架少用钢材1.1 t,节省用钢量17.5%;双竖杆空腹桁架结构高度更低,空腹部分及楼层有效空间更大;双竖杆空腹桁架的整体结构刚度、内力分布和技术经济性均优于传统空腹桁架,具有很好的工程应用前景。

关键词：空腹桁架等效惯性矩等效剪切刚度参数化分析经济性

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Selected aptamer specially combing 5-8F cells based on automatic screening instrument

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Chinese Chemical Letters 2022年第9期33卷 4208-4212页

作者： Zhukang Guo Baijiang Jin Yile Fang Yan Deng Zhu chen Hui chen Song Li Franklin Wang-Ngan Chow Polly H.M.Leung hanming Wang Lei Cai Nongyue He State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Department of Health Technology and Informatics The Hong Kong Polytechnic UniversityHong KongChina Guangzhou Wondfo Biotech Co. Ltd.Guangzhou 510641China Guangzhou Wondfo iCubate Biotech Co. Ltd.Guangzhou 510641China

Since the concept of aptamer emerged,many scientists have launched a rich field of research around it.However,few nucleic acids aptamer which use cell as target can be put into practical applications.We believe that a great deal of this lies in the complexity and irreproducibility of aptamer screening experiments themselves.The complexity is due to the cumbersome processes and the technical requirements for laboratory personnel,whereas irreproducibility arises from the fact that the starting point of such screens is nucleic acid libraries with random fragments,and that different libraries directly determine the differences or even the success or failure of screening results.The complexity and irreproducibility mentioned above,in turn,lead to the inability of this experiment to unfold on a large scale,which naturally cannot lead to excellent results for practical applications.In response to this problem,our group has developed an instrument for automated screening of tumor cell nucleic acid aptamers and characterized the properties of nucleic acid aptamers obtained using this instrument in a comprehensive manner.

关键词： Aptamer Cell-SELEX Automatic instrument Tumor Efficient screening

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(N,F)-codoped TiO_2 Nanocrystals as Visible Light-activated Photocatalyst

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Journal of Materials Science & Technology 2009年第1期25卷 73-76页

作者： Yanling Meng Jiansong chen Ying Wang hanming Ding Yongkui Shan Department of Chemistry East China Normal University Shanghai 200062 China

（N, F）-codoped anatase TiO2 nanocrystals with active visible light response were prepared by using a simple sol-gel approach. X-ray photoelectron spectroscopy measurements suggested that the substitutional N and F species replaced the lattice oxygen atoms in TiO2 nanocrystals. Such nanocrystals showed strong absorption from 400 to 550 nm, which was mainly induced by nitrogen doping. The phase transformation from anatase to rutile was hindered by fluorine doping at high calcination temperatures, which was verified by XRD patterns. The N2 adsorption-desorption isotherms revealed the absence of mesopores in these nanocrystals. The （N, F）- codoped TiO2 nanocrystals showed satisfying photocatalytic activity on the photo-degradation of methylene blue under visible light.

关键词： TiO2 nanocrystal （N, F）-codoping Sol-gel method Visible light photocatalyst

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分时相低对比剂注射方案在颅内动脉瘤三维-数字减影血管造影成像的应用

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岭南心血管病杂志 2024年第1期30卷 28-32页

作者：刘勇东秦琨贾乾君黄美萍魏志华董旭东蒙汉明陈光忠南方医科大学附属广东省人民医院(广东省医学科学院)介入导管室广州510080 南方医科大学附属广东省人民医院(广东省医学科学院)神经外科广州510080

目的探讨分时相低剂量低流率对比剂注射方案在颅内动脉瘤三维(three-dimensional,3D)-数字减影血管造影(digital subtraction angiography,DSA)成像的临床应用价值。方法前瞻性连续收集经计算机断层扫描血管成像(CTA)/磁共振血管造影(... 详细信息

目的探讨分时相低剂量低流率对比剂注射方案在颅内动脉瘤三维(three-dimensional,3D)-数字减影血管造影(digital subtraction angiography,DSA)成像的临床应用价值。方法前瞻性连续收集经计算机断层扫描血管成像(CTA)/磁共振血管造影(MRA)检查疑似或确诊颅内动脉瘤患者共69例,按照交替序贯将患者分实验组35例,常规组34例,统计分析两组患者动脉瘤成像评分、旋转造影辐射剂量及栓塞效果。结果实验组与常规组三维重建成像对颅内动脉瘤的细节显示比较,差异无统计学意义(P=0.633)。两组间患者辐射剂量空气比释动能(air kerma,AK)、剂量-面积乘积(dose-area product,DAP)比较,差异均无统计学意义(P=0.176,P=0.765)。两组患者间栓塞效果比较,差异无统计学意义(P=0.111)。实验组患者行3D-DSA使用对比剂剂量显著少于常规组,差异有统计学意义[(9.29±0.86)mL vs.(18.00±0.00)mL,P<0.01]。结论分时相低对比剂注射方案在颅内动脉瘤患者3D-DSA成像安全有效,应用该方案能显著减少患者的对比剂使用剂量。

关键词：颅内动脉瘤对比剂旋转血管造影三维重建

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Wellbore temperature distribution during drilling of natural gas hydrate formation in South China sea

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Petroleum 2021年第4期7卷 451-459页

作者： Xiaolong Zhao Yizhong Zhao Zenglin Wang Gang chen Peng Li Wei Liang Xuefeng Gao hanming Xu Lin Jiang Na Wei Research Institute of Petroleum Engineering Shengli OilfieldSINOPECDongying257000China Shengli Oilfield Company SINOPECDongying257000China State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500China State Key Laboratory of Natural Gas Hydrate Beijing10027China

The natural gas hydrate resources in the South China Sea alone are about 85 trillion cubic meters.In the drilling process of marine gas hydrate,the natural gas hydrate will decompose and produce gas,as the rising of temperature and dropping of the pressure in the annulus.This process will have a significant impact on drilling safety.Therefore,it is necessary to study the wellbore temperature distribution during the drilling of marine hydrate layer.In this paper,the wellbore temperature distribution of safe drilling in hydrated formation is taken as the research goal,and the research status of relevant domestic and international wellbore temperatures was investigated.According to the characteristics of the marine environment and reservoir-forming characteristics of hydrate reservoirs in the South China Sea,the wellbore temperature distribution model of offshore drilling wellbore under the condition of hydrate decomposition was established.The temperature distribution curve of drilling straight wellbore in hydrate layer of South China Sea was obtained.When drilling the hydrate reservoir,the distribution regularity of the wellbore temperature is similar to that of the conventional offshore drilling wellbore.However,the temperature of the wellbore annulus near the hydrate decomposition site is lower than the ambient temperature,mainly due to the hydrate decomposition endothermic.In this paper,the sensitivity analysis of several main parameters of the wellbore temperature distribution of drilling straight wellbore in hydrate layer of South China Sea was carried out.Through the conduction of experiment and numerical simulation,we have get some new findings:(1)The hydrate saturation has little effect on the wellbore temperature;(2)As the drilling fluid displacement increases,the annulus temperature of the wellbore above the mudline increases,and the temperature of the wellbore below the mudline decreases continuously;(3)As the density of the drilling fluid increases,the temperature at the wellhead decreases,and the

关键词： Wellbore temperature Natural gas hydrate South China Sea Numerical simulation Sensitivity analysis

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基于差分算法优化的自复位粒子滤波算法

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华南理工大学学报（自然科学版） 2023年第3期51卷 133-145页

作者：文尚胜丘志强许函铭陈贤东华南理工大学材料科学与工程学院广东广州510640

粒子滤波器作为常用的非高斯非线性的滤波方法,已成功地应用于各种工程领域。然而传统的重采样方法导致了粒子贫化的问题,严重降低了滤波估计的精度与鲁棒性。文中提出一种结合跟踪失败检测与改进差分优化融合的自复位粒子滤波方法。首... 详细信息

粒子滤波器作为常用的非高斯非线性的滤波方法,已成功地应用于各种工程领域。然而传统的重采样方法导致了粒子贫化的问题,严重降低了滤波估计的精度与鲁棒性。文中提出一种结合跟踪失败检测与改进差分优化融合的自复位粒子滤波方法。首先通过跟踪失败识别方法对滤波估计值进行初步检验,在正常跟踪时不启用优化策略,算法性能与标准粒子滤波无异;在跟踪失败时,通过差分算法对粒子集进行复位,复位过程中设置了粒子置信区间的上下界以防粒子过度集中,并结合检验指示值规避对粒子的多次优化,以缩短算法的估计时间。仿真结果表明,文中算法通过动态调节方式继承了标准粒子滤波和差分进化粒子滤波的优点,有效提高了滤波估计的鲁棒性与估计精度,可在滤波成功时避免启用优化策略以降低算法的整体时间复杂度,并在滤波失败时启用差分优化策略进行自我复位以提高算法估计精度;且在相同定位精度下,其所需粒子数较标准粒子滤波更少,整体时耗较差分进化粒子滤波更低,在建模不确定时也可表现出良好的效果。

关键词：粒子滤波差分进化算法自适应非线性滤波自复位

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