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RETINOBLASTOMA RELATED 1 switches mitosis to meiosis in rice

Plant Communications 2024年第6期5卷 193-212页

作者： Yongjie Miao hanli you Huixin Liu Yangzi Zhao Jiangzhe Zhao Yafei Li Yi Shen Ding Tang Baohui Liu Kewei Zhang Zhukuan Cheng Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Innovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education Agricultural College of Yangzhou UniversityYangzhou 225009China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants Department of BiologyCollege of Life SciencesZhejiang Normal UniversityJinhua 321004China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009China

The transition from mitosis to meiosis is a critical event in the reproductive development of all sexually reproducing ***,the mechanisms that regulate this process in plants remain largely ***,we find that the rice(Oryza sativa L.)protein RETINOBLASTOMA RELATED 1(RBR1)is essential to the transition from mitosis to *** of RBR1 function results in hyper-proliferative sporogenous-celllike cells(SCLs)in the anther locules during early stages of reproductive *** hyperproliferative SCLs are unable to initiate meiosis,eventually stagnating and degrading at late developmental stages to form pollen-free *** results suggest that RBR1 acts as a gatekeeper of entry into ***,cytokinin content is significantly increased in rbr1 mutants,whereas the expression of type-B response factors,particularly LEPTO1,is significantly *** the known close association of cytokinins with cell proliferation,these findings imply that hyper-proliferative germ cells in the anther locules may be attributed to elevated cytokinin concentrations and disruptions in the cytokinin *** a genetic strategy,the association between germ cell hyper-proliferation and disturbed cytokinin signaling in rbr1 has been *** summary,we reveal a unique role of RBR1 in the initiation of meiosis;our results clearly demonstrate that the RBR1 regulatory module is connected to the cytokinin signaling pathway and switches mitosis to meiosis in rice.

关键词： RETINOBLASTOMA RELATED1 meiosis initiation cytokinin rice

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Extensive sequence divergence between the reference genomes of Taraxacum kok-saghyz and Taraxacum mongolicum

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Science China(Life Sciences) 2022年第3期65卷 515-528页

作者： Tao Lin Xia Xu Huilong Du Xiuli Fan Qingwen Chen Chunyan Hai Zijian Zhou Xiao Su Liquan Kou Qiang Gao Lingwei Deng Jinsheng Jiang hanli you Yihua Ma Zhukuan Cheng Guodong Wang Chengzhi Liang Guomin Zhang Hong Yu Jiayang Li State Key Laboratory of Plant Genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Agrobiotechnology College of HorticultureChina Agricultural UniversityBeijing 100193China University of Chinese Academy of Sciences Beijing 100049China Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Biotechnology Research Institute Heilongjiang Academy of Agricultural SciencesHarbin 150086China Guangdong Laboratory of Lingnan Modern Agriculture Guangzhou 510642China School of Life Sciences Institute of Life Sciences and Green DevelopmentHebei UniversityBaoding 071002China

Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing ***,the genomic basis underlying natural rubber(NR)biosynthesis still needs more ***,we presented high-quality genome assemblies of rubber-producing ***-saghyz TK1151 and non-rubber-producing *** *** analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two *** gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene *** genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to *** study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.

关键词： reference genome Taraxacum kok-saghyz Taraxacum mongolicum comparative genomics natural rubber biosynthesis pathway

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青杄MYB转录因子基因PwMYB20的克隆及表达分析

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林业科学 2017年第5期53卷 23-32页

作者：游韩莉袁义杭李长江张凌云北京林业大学省部共建森林培育与保护教育部重点实验室北京100083

【目的】MYB转录因子家族是植物中最大的一类转录因子,在植物生长发育及抗逆调控网络中发挥重要作用。对青杄中MYB同源基因Pw MYB20的克隆与分析,有利于进一步探究Pw MYB20在植物生长发育及逆境响应中的功能,挖掘与利用青杄中的优质基... 详细信息

【目的】MYB转录因子家族是植物中最大的一类转录因子,在植物生长发育及抗逆调控网络中发挥重要作用。对青杄中MYB同源基因Pw MYB20的克隆与分析,有利于进一步探究Pw MYB20在植物生长发育及逆境响应中的功能,挖掘与利用青杄中的优质基因。【方法】采用RACE-PCR技术,从青杄c DNA文库中克隆得到Pw MYB20基因,并通过PCR技术克隆验证。利用Prot Param、Prot Scale、Fold Index等生物信息学软件对Pw MYB20理化性质进行分析预测。通过BLAST在线工具得到植物同源蛋白,并对其进行比对分析和进化树分析。采用实时荧光定量PCR技术分析Pw MYB20基因在不同组织中的表达性,以及干旱、低温、盐、ABA等非生物逆境胁迫处理后的表达变化。通过亚细胞定位及转录激活活性验证试验,揭示其生物学特性。【结果】通过RACE-PCR克隆得到Pw MYB20 c DNA全长966 bp,含675 bp的完整开放阅读框,编码225个氨基酸。Prot Param工具计算蛋白分子式为C1104H1740N340O330S8,分子质量为25.3 k Da,等电点为9.11;Protscale工具疏水性分析发现,Pw MYB20的疏水位点与亲水位点均匀分布,推测该蛋白为亲水蛋白;Signal P工具预测发现该蛋白没有信号肽结构域;利用Fold Index工具对蛋白质固有无序化进行分析,结果表明该蛋白固有无序化序列较多,推测在生理环境下蛋白的动态活性较大;TMHMM工具预测发现该蛋白没有跨膜结构域。通过对比分析发现Pw MYB20属于MYB家族基因,编码1个R2R3-MYB蛋白。进化树分析结果显示,青杄Pw MYB20与白云杉Pg MYB20聚为一簇。实时荧光定量PCR结果表明,Pw MYB20在种子中的表达量最高,其次是在针叶中,在花粉中的表达相对较少。Pw MYB20对干旱、4℃和ABA处理均有响应,而对Na Cl处理响应相对较弱。在干旱处理下,Pw MYB20表达量先上升后下降;4℃低温处理3 h和12 h时Pw MYB20的表达量上升,在4℃处理6 h时存在波动,呈现上升—下降—上升的趋势;Pw MYB20的表达受ABA处理持续诱导。亚细胞定位分析表明,Pw MYB20是一个主要定位于细胞核中的蛋白质。转录激活活性分析结果显示,Pw MYB20的C端存在转录激活活性,而Pw MYB20全长及其N端没有转录激活活性。【结论】青杄Pw MYB20,作为一个转录因子发挥作用,其转录激活活性位于C端;受干旱、低温和ABA诱导,普遍参与了植物应对逆境胁迫的响应过程。

关键词：青杄 MYB转录因子基因克隆胁迫响应基因表达

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青杄转录因子PwHAP5及其同源蛋白AtHAP5下游靶基因的鉴定

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东北林业大学学报 2020年第6期48卷 6-12,16页

作者：苗雅慧游韩莉郜银涛张凌云森林培育与保护教育部重点实验室(北京林业大学) 北京100083

利用分子生物学手段,对青杄PwHAP5及其拟南芥同源基因AtHAP5的下游调控基因进行探究,旨在丰富青杄以及拟南芥中HAP5转录因子的调控网络。通过转录激活活性分析,发现PwHAP5和AtHAP5均无转录激活活性。两个酵母单杂交体系(pHis2和pAbAi体... 详细信息

利用分子生物学手段,对青杄PwHAP5及其拟南芥同源基因AtHAP5的下游调控基因进行探究,旨在丰富青杄以及拟南芥中HAP5转录因子的调控网络。通过转录激活活性分析,发现PwHAP5和AtHAP5均无转录激活活性。两个酵母单杂交体系(pHis2和pAbAi体系酵母单杂交实验)显示A37、HSFA2启动子全长、只含有CCAAT box的区域均不能与PwHAP5和AtHAP5直接结合。EMSA试验结果表明,AtHAP5与A37和HSFA2启动子在体外不能直接结合;单(双)荧光素酶试验结果表明,AtHAP5对HSFA2具有负调控作用,但对A37表达无影响。通过RT-qPCR试验检测在异源过表达OE-PwHAP5植株中预测靶基因的表达量,结果显示A37与HSFA2相对表达量均无显著变化,而在同源过表达植株OE-AtHAP5中,HSFA2的相对表达量下降。这些结果显示AtHAP5在体内负调控HSFA2表达,而PwHAP5在进化上与AtHAP5的功能存在差异。

关键词：青杄转录因子 PwHAP5 AtHAP5 HSFA2

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杜仲花粉离体萌发特征及花粉管微丝骨架分布

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西北植物学报 2014年第3期34卷 495-501页

作者：游韩莉贾婷婷田菊王君康向阳北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室北京100083 北京林业大学林学院北京100083 北京林业大学生物科学与技术学院北京100083

以干燥的杜仲成熟花粉为材料,对杜仲花粉离体萌发的适宜液体培养基配方进行了筛选,并对花粉萌发特征及花粉管微丝骨架分布规律进行了研究。结果表明:(1)适宜杜仲花粉离体萌发的液体培养基组成为200g/L蔗糖+30mg/L硼酸+10mg/L Ca(NO3)2,... 详细信息

以干燥的杜仲成熟花粉为材料,对杜仲花粉离体萌发的适宜液体培养基配方进行了筛选,并对花粉萌发特征及花粉管微丝骨架分布规律进行了研究。结果表明:(1)适宜杜仲花粉离体萌发的液体培养基组成为200g/L蔗糖+30mg/L硼酸+10mg/L Ca(NO3)2,于26℃条件下离体培养18h的花粉萌发率可达46.29%±3.75%。(2)在适宜液体培养条件下,杜仲花粉萌发率在培养6h内急剧增长,随后趋于平稳;而花粉管在培养8h内伸长较快,之后有放缓趋势,至培养48h时,花粉管长度可达363.14±30.51μm。(3)杜仲花粉属于2胞花粉,花粉萌发过程中,营养核和生殖核的移动存在一定的时序性,通常营养核先于生殖核进入到花粉管;杜仲花粉生殖核的有丝分裂发生在花粉管中,离体培养12h可逐渐观察到有丝分裂行为。(4)花粉萌发过程,微丝骨架形成束状,与花粉管伸长方向平行排布,与较为稀疏的网状微丝阵列组成连续系统,引导细胞核的运动。

关键词：杜仲离体液体培养基花粉萌发微丝骨架

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Large-scale video compression： recent advances and challenges

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Frontiers of Computer Science 2018年第5期12卷 825-839页

作者： Tao TIAN hanli WANG Department of Computer Science and Technology Tongji University Shanghai 201804 China Key Laboratory of Embedded System and Service Computing Ministry of Education Tongji University Shanghai 200092 China Shanghai Engineering Research Center of Industrial Vision Perception & Intelligent Computing Shanghai 200092 China

The evolution of social network and multimedia technologies encourage more and more people to generate and upload visual information, which leads to the generation of large-scale video data. Therefore, preeminent compression technologies are highly desired to facilitate the storage and transmission of these tremendous video data for a wide variety of applications. In this paper, a systematic review of the recent advances for large-scale video compression （LSVC） is presented. Specifically, fast video coding algorithms and effective models to improve video compression efficiency are introduced in detail, since coding complexity and compression efficiency are two important factors to evaluate video coding approaches. Finally, the challenges and fu- ture research trends for LSVC are discussed.

关键词： large-scale video compression fast video coding compression efficiency

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Assessment of future climate change impacts on water-heat-salt migration in unsaturated frozen soil using CoupModel

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Frontiers of Environmental Science & Engineering 2021年第1期15卷 145-161页

作者： hanli Wan Jianmin Bian Han Zhang Yihan Li College of New Energy and Environment Jilin UniversityChangchun 130021China Key Laboratory of Groundwater Resources and Environment(Ministry of Education) Jilin UniversityChangchun 130021China

The transport mechanisms of water,heat,and salt in unsaturated frozen soil,as well as its response to future climate change are in urgent need of *** this study,western Jilin Province in north-eastern China was studied to produce a model of coupled water-heat-salt in unsaturated frozen soil using *** water,heat,and salt dynamics of unsaturated frozen soil under three representative concentration pathway(RCP)scenarios were simulated to analyze the effects of future climate change on unsaturated frozen *** results show that water,heat,and salt migration are tightly coupled,and the soil salt concentration in the surface layer(10 cm)exhibits explosive growth after freezing and *** future(2020–2099)meteorological factors in the study area were predicted using the Statistical Downscaling Model(SDSM).For RCP2.6,RCP4.5,and RCP8.5 scenarios,future temperatures during the freeze–thaw period increased by 2.68°C,3.18°C,and 4.28°C,respectively;precipitation increased by 30.28 mm,28.41 mm,and 32.17 mm,respectively;and evaporation increased by 93.57 mm,106.95 mm,and 130.57 mm,*** change will shorten the freeze–thaw period,advance the soil melting time from April to March,and enhance water and salt *** to the baseline period(1961–2005),future soil salt concentrations at 10 cm increased by 1547.54 mg/L,1762.86 mg/L,and 1713.66 mg/L under RCP2.6,RCP4.5,and RCP8.5,*** explosive salt accumulation is more *** measures should be taken to prevent the salinization of unsaturated frozen soils and address climate change.

关键词： Soil salinization Climate change Unsaturated frozen soil Water-heat-salt balance Soil environment change

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Highly oxidized sesquiterpenoids from Parasenecio rubescens and assessment of their cytotoxicity

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Chinese Journal of Natural Medicines 2024年第11期22卷 1020-1029页

作者： JIN An DUAN Fangfang RUAN hanli Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation School of PharmacyTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China School of Pharmaceutical Sciences Hunan University of MedicineHuaihua 418000China

A phytochemical investigation of the whole plant of Parasenecio rubescens(***)*** yielded 14 previ-ously undescribed,highly oxidized bisabolane-type sesquiterpenoids,named pararunines L-Y,along with one known oplopane-type *** structural elucidation of these compounds was accomplished through comprehensive spectroscopic analysis,in-cluding nuclear magnetic resonance(NMR)and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)*** by traditional uses and previous studies on this genus,all isolated compounds were subjected to in vitro cytotoxicity assays against four human cancer cell lines(MCF-7,Hela,HCT116,and HT-29).Considering that the reported chemical constituents of nu-merous other species within this genus primarily consist of eremophilane-type sesquiterpenoids,our findings not only expand the struc-tural diversity of bisabolane-type sesquiterpenoids but also contribute valuable scientific evidence to the chemotaxonomy of this genus.

关键词： Parasenecio rubescens Asteraceae Bisabolane-type sesquiterpenoids Oplopane-type sesquiterpenoid Cytotoxic activity

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A filtering-based bridge weigh-in-motion system on a continuous multi-girder bridge considering the influence lines of different lanes

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Frontiers of Structural and Civil Engineering 2020年第5期14卷 1232-1246页

作者： hanli WU Hua ZHAO Jenny LIU Zhentao HU Key Laboratory for Wind and Bridge Engineering of Hunan Province College of Civil EngineeringHunan UniversityChangsha 410082China Department of Civil Architectural and Environmental EngineeringMissouri University of Science and TechnologyRollaMO 65409USA Qingyuan Traffic and Transportation Bureau Qingyuan 511500China

A real-time vehicle monitoring is crucial for effective bridge maintenance and traffic management because overloaded vehicles can cause damage to bridges,and in some extreme cases,it will directly lead to a bridge *** weigh-in-motion(BWIM)system as a high performance and cost-effective technology has been extensively used to monitor vehicle speed and weight on ***,the dynamic effect and data noise may have an adverse impact on the bridge responses during and immediately following the vehicles pass the *** fast Fourier transform(FFT)method,which can significantly purify the collected structural responses(dynamic strains)received from sensors or transducers,was used in axle counting,detection,and axle weighing technology in this *** further improve the accuracy of the BWIM system,the field-calibrated influence lines(ILs)of a continuous multi-girder bridge were regarded as a reference to identify the vehicle weight based on the modified Moses algorithm and the least squares *** situ experimental results indicated that the signals treated with FFT filter were far better than the original ones,the efficiency and the accuracy of axle detection were significantly improved by introducing the FFT method to the BWIM ***,the lateral load distribution effect on bridges should be considered by using the calculated average ILs of the specific lane individually for vehicle weight calculation of this lane.

关键词： bridge weigh-in-motion continuous bridge fast Fourier transform influence line axle weight calculation

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Intensity Correlation Function of a Single-Mode Laser Driven by Two Colored Noises with Colored Cross-Correlation

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Communications in Theoretical Physics 2004年第1X期42卷 59-63页

作者： hanli-Bo CAOLi WUDa-Jin WANGJun StateKeyLaboratoryofLaserTechnology HuazhongUniversityofScienceandTechnologyWuhan430074China CCAST(WorldLaboratory) P.O.Box8730Beijing100080China DepartmentofPhysics HuazhongUniversityofScienceandTechnologyWuhan430074China

By using the linear approximation method, the intensity correlation function and the intensity correlation time are calculated in a gain-noise model of a single-mode laser driven by colored cross-correlated pump noise and quantum noise, each of which is colored. We detect that, when the cross-correlation between both noises is negative, the behavior of the intensity correlation function C(t) versus time t, in addition to decreasing monotonously, also exhibits several other cases, such as one maximum, one minimum, and two extrema (one maximum and one minimum), i.e., some parameters of the noises can greatly change the dependence of the intensity correlation function upon time. T3.

关键词：强度相关函数强度相关时间线性近似理论双色噪声单模激光器激光物理

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