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塑料中PAEs环境污染物向食品模拟物中迁移及对CYP3A4和芳香化酶的影响

第三届全国生物入侵大会

作者： Jian-yang Guo~(1,2),Sheng-zhang Dong~3,Shu-sheng Liu~2,Gong-yin Ye~(2*) 1.State key laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100081,china 2.Ministry of Agriculture key laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences,zhejiang university,hangzhou 310029,china 3.zhejiang provincial key laboratory of biometrology and inspection and quarantine,College of Life Sciences,china jiliang university,hangzhou,zhejiang 310018,china.

Whiteflies are cosmopolitan phloem-feeding pests that cause serious damage to many crops worldwide due to direct feeding and vectoring of many plant *** B whitefly Bemisia tabaci (Gennadius) is one of the most widespread and damaging whitefly *** interactions of begomoviruses and reproduction of their vectors are not clearly *** this study,Vitellin(Vt) from the begomoviruses vector B whitefly was purified from egg extracts by the combined use of gel filtration and ion-exchange *** Vt had a native molecular mass of 380 kDa and composed of two subunits with approximate molecular weights of 190 *** and polyclonal anti-Vt antibodies,which cross-reacted equally with yolk protein in ovary(Vt) and hemolymph vitellogenin(Vg),were used to measure the kinetics of Vg *** showed increased synthesis of Vg prior to detachment,and then a slight decrease of the Vg level until the 6th *** second peak level of Vg was observed at 12 d after ***,the level of vitellin in the ovaries began to increase at the pupal stage as the females began oogenesis and declined at 12 d after *** to the female adults fed on healthy plants,significant higher level of Vg/Vt was observed in females fed on TYLCCNV-infected *** studies provide the basis for future investigations on reproductive strategy of invasive whitefly.

关键词： Bemisia tabaci Vitellogenin Vitellin Vitellogenesis Oocyte Monoclonal antibody

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中国食品学报 2014年第11期14卷 165-170页

作者：葛建林芳邓同乐胡华军赵进刘洋王梦馨韩宝瑜中国计量学院生命科学学院杭州310018 浙江省生物计量及检验检疫技术重点实验室杭州310018

目的:探讨塑料中PAEs向食品模拟物中迁移的规律,阐明PAEs对代谢酶CYP3A4和芳香化酶表达水平的影响。方法:利用GC-FID建立不同食品模拟物中5种PAEs类化合物检测方法,检测不同食品模拟物中PAEs类化合物的迁移量。采用q RT-PCR技术检测PAE... 详细信息

目的:探讨塑料中PAEs向食品模拟物中迁移的规律,阐明PAEs对代谢酶CYP3A4和芳香化酶表达水平的影响。方法:利用GC-FID建立不同食品模拟物中5种PAEs类化合物检测方法,检测不同食品模拟物中PAEs类化合物的迁移量。采用q RT-PCR技术检测PAEs类化合物对肝脏代谢酶CYP3A4和睾丸间质细胞中芳香化酶m RNA转录水平的影响,采用荧光免疫组织化学技术分析对其蛋白表达的影响。结果:不同食品模拟物中5种PAEs类化合物迁移量不同,其中以异辛烷中迁移量最大,而且迁移量随着时间的延长而增加。PAEs类化合物显著诱导肝脏中代谢酶CYP3A4基因表达,而对睾丸中芳香化酶基因表达具有极显著的抑制作用。结论:PAEs类化合物在不同极性食品中迁移量不同,脂溶性越高迁移量越大;PAEs类化合物对肝脏CYP3A4和睾丸芳香化酶表达有显著影响,影响体内类固醇激素的浓度,从而表现出生殖内分泌毒性。

关键词： PAEs类化合物食品模拟物迁移量 CYP3A4基因表达芳香化酶基因表达

Research on the variation of the release of volatile organic compounds of the tea plant elicited by fed by Iragoides fasciata Moore(Lepidoptera: Eucleidae)

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Research on the variation of the release of volatile organic...

Insight into the effect of geographic location and intercropping on contamination characteristics and exposure risk of phthalate esters(PAEs) in tea plantation soils

中国第五届植物化感作用学术研讨会

作者： HUANG An-ping 1,2,3,han Bao-yu 2 & BAO Xiao-cun3 1Central South university,Changsha 410083,china 2.zhejiang provincial key laboratory of biometrology and inspection & quarantine of china jiliang university,hangzhou 310018,china 3Hunan Tea Research Institute ,Hunan Academy of Agricultural Sciences,Changsha 410125,china

Change in volatile organic compounds (VOCs) of Camellia sinensis (L.) *** damaged by Iragoides fasciata Moore was studied in this *** from undamaged and damaged tea plant were collected by simultaneous distillation extraction and analyzed using gas chromatography/mass spectrometry (GC-MS) ,The result indicated that a total of 63 components,mainly esters,alcohols,hydrocarbons,ketones,heterocyclic compounds,aldehydes ,organic acids and ethers were found in undamaged twigs and leaves ,The relative amount of hydrocarbons is 5.83% while those from damaged ones 97.15%.Furthermore,32 new compounds were found,including 10 kinds of ester,5 of alcohol,5 of olefin ,4 of heterocyclic,3 of aromatic hydrocarbons,1 of alkane,1 of ketone ad 1 of *** results can lay a theoretical foundation for Screening infochemicals which can serve as a lure for natural enemies of insect and applying it to control of Iragoides fasciata Moore.

关键词： Camellia sinensis (L.) O. Kuntze Iragoides ragoides fasciata Moore volatile organic compounds (VOCs) herbivore-induced plant volatiles (HIPVs)

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Journal of Integrative Agriculture 2024年第11期23卷 3896-3911页

作者： Jie Li Shanjie han Ruhang Xu Xuchen Zhang Junquan Liang Mengxin Wang Baoyu han zhejiang provincial key laboratory of biometrology and inspection and quarantine College of Life SciencesChina Jiliang University hangzhou Tea & Chrysanthemum Technology Co.Ltd.

Phthalate esters(PAEs) are an emerging pollutant due to widespread distribution in environmental mediums that have attracted widespread attention over recent years. However, there is little information about tea plantation soil PAEs. A total of 270 soil samples collected from 45 tea plantations in the major high-quality tea-producing regions of Jiangsu, zhejiang, and Anhui provinces in china were analyzed for seven PAEs. The detection frequency of PAEs in tea plantation soil was 100%. DBP, DEHP, and DiBP were the main congeners in tea plantation soil. The PAEs concentrations in the upper soil were significantly higher than those in the lower soil. The concentration of tea plantation soil PAEs in Jiangsu Province was significantly lower than those in zhejiang and Anhui provinces. Intercropping with chestnuts can effectively reduce the contamination level of PAEs in tea plantation soil. Correlation analysis, redundancy analysis, partial correlation analysis, and structural equation modeling methods further confirmed the strong direct influence of factors such as chestnut–tea intercropping, temperature, and agricultural chemicals on the variation of PAEs in tea plantation soil. The health and ecological risk assessments indicated that non-carcinogenic risk was within a safe range and that there was a high carcinogenic risk via the dietary pathway, with DBP posing the highest ecological risk.

关键词： phthalate esters chestnut and tea intercropping driving factors spatial heterogeneity risk assessment

一种灰茶尺蠖高效引诱剂的研制及其田间防效评价

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生态学报 2024年第6期44卷 2597-2608页

作者：韩宝瑜黄光政韩善捷王梦馨中国计量大学生命科学学院浙江省生物计量与检验检疫技术重点实验室杭州310018 杭州茶菊科技有限公司杭州310018

为研制有效诱杀重要害虫灰茶尺蠖雌、雄成虫的性信息素/植物源信息物复合型性诱剂,选源自茶梢和茶花挥发物的30个主要成分,分别配成10^(-6)g/mL、10^(-4)g/mL和10^(-2)g/mL液体石蜡溶液作味源,检测其引起1日龄灰茶尺蠖雌、雄成虫的EAG... 详细信息

为研制有效诱杀重要害虫灰茶尺蠖雌、雄成虫的性信息素/植物源信息物复合型性诱剂,选源自茶梢和茶花挥发物的30个主要成分,分别配成10^(-6)g/mL、10^(-4)g/mL和10^(-2)g/mL液体石蜡溶液作味源,检测其引起1日龄灰茶尺蠖雌、雄成虫的EAG反应值。结果表明:(1)每种味源的3个剂量处理皆引起明显的EAG反应;随着味源浓度增加,EAG反应值显著增大;(2)引起的雌、雄成虫EAG反应值相近,差异不显著;(3)芳樟醇、反-2-己烯醛、顺-3-己烯-1-醇、己醇、苯乙醇和6-甲基-5-庚烯-2-酮的每个剂量处理引起强烈的EAG反应。将这6种EAG活性成分与灰茶尺蠖2个主要的性信息素成分Z,Z,Z-3,6,9-十八碳三烯和Z,Z-3,9-6,7-环氧-十八碳二烯配成数个备选的复合型性诱剂,载于羊毛毡细条上、塑封、制成诱芯,在茶园中检测其引诱活性,发现诱效最优的复合型性诱剂在每个诱芯上组成为:6种EAG活性成分各2 mg、Z,Z,Z-3,6,9-十八碳三烯0.4 mg、Z,Z-3,9-6,7-环氧-十八碳二烯0.6 mg、抗氧化剂——2,6-二叔丁基-4-甲基苯酚1 mg、正己烷100μL。此外,使用土黄、荧光黄、米黄、素馨黄、桔黄、土褐、墨绿、芽绿、草绿、荧光绿、天蓝、大红和纯白等13种色板诱杀灰茶尺蠖成虫,发现其偏嗜暗黄色,以土黄色效果最佳。遂将该诱芯载于土黄色粘板上组成诱捕器,诱杀防治灰茶尺蠖主害代——第3代,试验程序:在第2代羽化始盛期用诱捕器诱杀成虫以减少交尾几率而压低第3代幼虫数,在第3代初龄幼虫期喷施2.5%联苯菊酯、茶尺蠖核型多角体病毒·苏云金杆菌悬浮剂(1×10^(4)PIB·2000 IU/μL),接着定期调查三者的防治效果,结果表明复合型性诱剂的防效显著优于后两者。该研究为灰茶尺蠖的绿色防控提供了可敷应用的技术产品。

关键词：灰茶尺蠖复合型性诱剂触角电位活性成分性信息素诱杀

花期杭白菊植株引诱菊小长管蚜的嗅觉和视觉线索及应用

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生态学报 2024年第6期44卷 2609-2620页

作者：曹原韩善捷李捷黄光政韩宝瑜王梦馨中国计量大学生命科学学院浙江省生物计量与检验检疫技术重点实验室杭州310018 杭州茶菊科技有限公司杭州310018

杭白菊是一类顶级的茶用菊,原产地是浙江省桐乡市。菊小长管蚜Macrosiphoniella sanborni是菊属植物重要害虫,常常在花期觅食于花上和匿于花内,随着菊花采摘、加工而残留在菊花产品中。冲饮菊花茶过程中,蚜尸或肢体就浮现在菊花茶汤中,... 详细信息

杭白菊是一类顶级的茶用菊,原产地是浙江省桐乡市。菊小长管蚜Macrosiphoniella sanborni是菊属植物重要害虫,常常在花期觅食于花上和匿于花内,随着菊花采摘、加工而残留在菊花产品中。冲饮菊花茶过程中,蚜尸或肢体就浮现在菊花茶汤中,致饮者“倒胃口”。为揭示该蚜嗜好花朵原因和研制有效的防控手段以便在花期控制该蚜,分离鉴定了花期杭白菊植株的36种挥发性化合物,挑选其中含量大的、主要的17种用作味源,对该蚜做行为测定。发现a-蒎烯、反-2-己烯醛、(+)-4-蒈烯、顺-3-己烯乙酯、顺式-β-罗勒烯、γ-萜品烯、或者1-辛烯-3-乙酯7种成分的10-4g/mL的正己烷溶液显著地吸引该蚜雌、雄成蚜。依次将该7种成分的每种10-2g/mL正己烷溶液与蚜虫性信息素荆芥内酯的10-4g/mL正己烷溶液按照60∶10∶10∶2∶2∶20∶2∶1体积比配制成复合型蚜虫引诱剂,在菊园中与专利产品蚜虫引诱剂比较诱效,结果表明前者对于该蚜更具有引诱力。此外,在杭白菊园进行的该蚜趋色行为测定中,测得菊花黄对该蚜诱效稍强于芽绿色。随后,将每块菊花黄粘板附1个复合型蚜虫引诱剂诱芯,组成1个诱捕器,在杭白菊花期的菊园放置相互间距7 m×8 m的诱捕器诱杀成蚜。结果证实:不仅每个诱捕器诱杀了大量菊小长管蚜成虫,而且诱捕器的防效优于吡虫啉药液。认为:花期杭白菊植株的主要挥发性化合物和花朵上雌蚜释放的性信息素(嗅觉线索)与菊花的黄色(视觉线索)叠加引诱了菊小长管蚜;研制的载有复合型蚜虫引诱剂的菊花黄粘板可用作花期有效诱杀菊小长管蚜的手段。

关键词：杭白菊开花期菊小长管蚜嗅觉和视觉线索诱杀

Exploiting push-pull strategy to combat the tea green leafhopper based on volatiles of Lavandula angustifolia and Flemingia macrophylla

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Journal of Integrative Agriculture 2020年第1期19卷 193-203页

作者： han Shan-jie WANG Meng-xin WANG Yan-su WANG Yun-gang CUI Lin han Bao-yu zhejiang provincial key laboratory of biometrology and inspection&quarantine College of Life SciencesChina Jiliang UniversityHangzhou 310018P.R.China Tea Research Institute Yunnan Academy of Agricultural SciencesMenghai 666201P.R.China

Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, Me SA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL^-1 in n-hexane, respectively;2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL^-1 in n-hexane, respectively;3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and Me SA at 2, 10, 10 and 11 mg mL^-1 in n-hexane, respectively. Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL^-1. For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps. The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards. Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components. When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 9

关键词： push-pull strategy attractants repellents Flemingia macrophylla tea green leafhopper tea plantation

Meat quality traits of four Chinese indigenous chicken breeds and one commercial broiler stock

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2013年第10期14卷 896-902页

作者： Rong-fa GUAN Fei LYU Xiao-qiang CHEN Jie-qing MA han JIANG Chao-geng XIAO zhejiang provincial key laboratory of biometrology and inspection and quarantine China Jiliang University College of Biological and Environmental Engineering Zhejiang University of Technology Hubei university of Technology Food Science Institute Zhejiang Academy of Agricultural Sciences

Meat quality traits of four genotypes of Chinese indigenous chicken [Ninghai chicken(NC),frizzle chicken(FC),Ninghai xiang chicken(XC),and Zhenning loquat chicken(LC)] and one genotype of commercial broiler [Arbor Acres plus broiler(AAB)] were *** indigenous chickens were raised before the commercial chickens in order to achieve the same final processed *** chickens of NC,FC,XC,and LC showed significantly higher inosine-5′-monophosphate(IMP) content,shorter fiber diameter,and lower shear force than those of AAB(Phan XC and LC(Phan FC and LC(P0.05).The indigenous chickens from FC displayed the highest total lipid content in the five bird genotypes(P<0.05).Significant differences of pH,color values of L* and a*,and drip loss for the five genotypes of birds were also *** conclusion,there were significant differences in the meat quality traits of the bird breeds selected in this study,and the indigenous chickens,especially the NC genotype,produced better quality meat as far as the IMP content,fiber diameters,and shear forces were concerned.

关键词： Indigenous chicken Commercial broiler Meat quality Fiber diameter Inosine-5′-monophosphate

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冬季遮阳网茶园昆虫趋色性及其群落结构分析

茶叶通讯 2024年第1期51卷 54-59页

作者：曹原李捷兰建军郭春伟徐伟众韩宝瑜中国计量大学生命科学学院/浙江省生物计量与检验检疫技术重点实验室浙江杭州310018 杭州茶菊科技有限公司浙江杭州310018 松阳县农业农村局浙江松阳323400 松阳县臻琳家庭农场浙江松阳323499 松阳县伟众家庭农场浙江松阳323406

茶园建设固定遮阳网可助力干旱时节抗旱和冬季防冻,但茶园病虫害也常发。目前,有关固定遮阳网茶园昆虫群落缺乏研究。以浙江松阳县建有的固定遮阳网茶园为对象,冬季采用13种颜色的诱虫色板每隔10 d依次对5块遮阳网茶园内昆虫进行诱捕,统... 详细信息

茶园建设固定遮阳网可助力干旱时节抗旱和冬季防冻,但茶园病虫害也常发。目前,有关固定遮阳网茶园昆虫群落缺乏研究。以浙江松阳县建有的固定遮阳网茶园为对象,冬季采用13种颜色的诱虫色板每隔10 d依次对5块遮阳网茶园内昆虫进行诱捕,统计24 h内捕获的种数和个体数。结果共捕获6个目37个科的2940个昆虫(蜘蛛);诱捕量最多的为双翅目,种数、个体数分别占总物种数、总个体数的29.7%和69.5%;聚类分析将13种色板中的荧光绿、芽绿、米黄、土黄、橘黄、草绿、荧光黄和素馨黄色板聚为一类,每种色彩的每块板平均诱捕的物种数和个体数分别是(19.4±2.4)种和(286.9±55.4)个;墨绿、大红、天蓝、土褐和纯白5种色板聚为一类,每种色彩的每块板平均诱捕的物种数和个体数分别是(11.8±3.3)种和(129.0±36.4)个;主要害虫有茶小绿叶蝉、茶蚜和黑刺粉虱等,主要天敌是尺蠖原绒茧蜂和单白绵副绒茧蜂。研究认为遮阳网茶园成为主要害虫和重要天敌的越冬庇护场所;芽绿、素馨黄、荧光绿和荧光黄等色板可作为监测茶园虫情的手段之一。

关键词：茶园昆虫趋色性群落结构遮阳网冬季

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