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Proteomic Analysis of Rice Leaves Shows the Different Regulations to Osmotic Stress and Stress Signals

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Journal of Integrative Plant Biology 2010年第11期52卷 981-995页

作者： Lie-Bo Shu wei Ding Jin-Hong Wu Fang-Jun Feng Li-Jun Luo han-wei mei Huazhong Agricultural University Wuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China

Following the idea of partial root-zone drying （PRD） in crop cultivation,the morphological and physiological responses to partial root osmotic stress （PROS） and whole root osmotic stress （WROS） were investigated in *** caused stress symptoms like leaf rolling and membrane *** stimulated stress signals,but did not cause severe leaf *** proteomic analysis,a total of 58 proteins showed differential expression after one or both treatments,and functional classification of these proteins suggests that stress signals regulate photosynthesis,carbohydrate and energy *** other proteins （anthranilate synthase and submergence-induced nickel-binding protein） were upregulated only in the PROS plants,indicating their important roles in stress ***,more enzymes were involved in stress defense,redox homeostasis,lignin and ethylene synthesis in WROS leaves,suggesting a more comprehensive regulatory mechanism induced by osmotic *** study provides new insights into the complex molecular networks within plant leaves involved in the adaptation to osmotic stress and stress signals.

关键词： PROTEOMICS CARRIER proteins SYMPTOMS METABOLISM MOLECULAR biology

Pathophysiological consequences and treatment strategy of obstructive jaundice

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World Journal of Gastrointestinal Surgery 2023年第7期15卷 1262-1276页

作者： Jun-Jian Liu Yi-Meng Sun Yan Xu han-wei mei Wu Guo Zhong-Lian Li Department of Hepatobiliary and Pancreatic Surgery Tianjin Medical University Nankai HospitalTianjin 300102China Graduate School Tianjin Medical UniversityTianjin 300070China Graduate School Tianjin University of Traditional Chinese MedicineTianjin 301617China

Obstructive jaundice(OJ)is a common problem in daily clinical ***,completely understanding the pathophysiological changes in OJ remains a challenge for planning current and future *** effects of OJ are widespread,affecting the biliary tree,hepatic cells,liver function,and causing systemic *** lack of bile in the intestine,destruction of the intestinal mucosal barrier,and increased absorption of endotoxins can lead to endotoxemia,production of proinflammatory cytokines,and induce systemic inflammatory response syndrome,ultimately leading to multiple organ dysfunction *** management of OJ includes adequate water supply and electrolyte replacement,nutritional support,preventive antibiotics,pain relief,and itching *** surgical treatment of OJ depends on the cause,location,and severity of the *** drainage,surgery,and endoscopic intervention are potential treatment options depending on the patient's *** addition to modern medical treatments,Traditional Chinese medicine may offer therapeutic benefits for OJ.A comprehensive search was conducted on PubMed for relevant articles published up to August *** review discusses in detail the pathophysiological changes associated with OJ and presents effective strategies for managing the condition.

关键词： Jaundice Obstructive Pathophysiology Treatment strategy Traditional Chinese medicine

Brain region-specific roles of brain-derived neurotrophic factor in social stress-induced depressive-like behavior

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Neural Regeneration Research 2025年第1期20卷 159-173页

作者： Man han Deyang Zeng wei Tan Xingxing Chen Shuyuan Bai Qiong Wu Yushan Chen Zhen wei Yufei mei Yan Zeng Brain Science and Advanced Technology Institute Wuhan University of Science and TechnologyWuhanHubei ProvinceChina Geriatric Hospital Affiliated to Wuhan University of Science and Technology WuhanHubei ProvinceChina School of Public Health Wuhan University of Science and TechnologyWuhanHubei ProvinceChina

Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral *** studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region–specific,particularly involving the corticolimbic system,including the ventral tegmental area,nucleus accumbens,prefrontal cortex,amygdala,and *** how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress *** this review,we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of *** focused on associated molecular pathways and neural circuits,with special attention to the brain-derived neurotrophic factor–tropomyosin receptor kinase B signaling pathway and the ventral tegmental area–nucleus accumbens dopamine *** determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature,severity,and duration of stress,especially in the above-mentioned brain regions of the corticolimbic ***,BDNF might be a biological indicator regulating stress-related processes in various brain regions.

关键词： amygdala chronic mild stress chronic social defeat stress corticolimbic system depression hippocampus medial prefrontal cortex nucleus accumbens social stress models ventral tegmental area

Panicle Water Potential,a Physiological Trait to Identify Drought Tolerance in Rice

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Journal of Integrative Plant Biology 2007年第10期49卷 1464-1469页

作者： Guo-Lan Liu han-wei mei Xin-Qiao Yu Gui-Hua Zou Hong-Yan Liu Ming-Shou Li Liang Chen Jin-Hong Wu Li-Jun Luo Huazhong Agricultural University Wuhan China430070 Shanghai Agrobiological Gene Center Shanghai China201106

Two upland rice varieties （IRAT109, IAPAR9） and one lowland rice variety （Zhenshan 97B） were planted in summer and treated with both normal （full water） and drought stress in the reproductive stage. Panicle water potential （PWP） and leaf water potential （LWP） were measured every 1.0-1.5 h over 24 h on sunny days. Both PWP and LWP of upland varieties started to decrease later, maintained a higher level and recovered more quickly than that of the lowland variety. The results show that PWP can be used as an indicator of plant water status based on the parallel daily changes, and the high correlation between PWP and LWP. Similar correlations were also observed between PWP, LWP and eight traits related to plant growth and grain yield formation. PWP seemed to be more effective for distinguishing the upland rice varieties with different drought-tolerant ability. Differences in PWP and LWP between upland and lowland rice varieties were also observed at noon even under normal water conditions, implying the incorporation of the drought-tolerant mechanism to improve the photosynthesis and yield of traditional paddy rice.

关键词： correlation analysis drought tolerance leaf water potential Oryza sativa panicle water potential.

Screening for Drought Resistance of Rice Recombinant Inbred Populations in the Field

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Journal of Integrative Plant Biology 2007年第10期49卷 1508-1516页

作者： Gui-Hua Zou Hong-Yan Liu han-wei mei Guo-Lan Liu Xin-Qiao Yu Ming-Sou Li Jing-Hong Wu Liang Chen Li-Jun Luo Shanghai Agrobiological Gene Center Shanghai China201106 Huazhong Agncultural University Wuhan China430070 Shanghai Jiao Tong University Shanghai China200030 Zhejiang Academy of Agricultural Sciences Hangzhou China310021

In a 2-year experiment, 187 genotypes were grown under well-watered and drought stress conditions, imposed at panicle initiation stage. The relationship of genotypic variation in yield under drought conditions to potential yield, heading date and flowering delay, reduction in plant height, and to a drought response index （DRI） was detected. Grain yield under drought stress conditions was associated with yield under well-watered conditions （r= 0.47^＊＊, and r= 0.61^＊＊ during 2 years of tests）. The delay of heading date ranged from -1 （no delay） to 24days, and was negatively associated with grain yield （r =-0.40^＊）, spikelet fertility percentage （r =-0.40^＊＊）, harvest index （r =-0.58^＊＊）, but positively associated with yield reduction percentage （r = 0.60^＊＊）. The reduction in plant height was negatively associated with grain yield （r =-0.24^＊＊, and r=-0.29^＊＊）, spikelet fertility percentage （r = -0.23^＊＊, and r= -0.21^＊）, harvest index （r = -0.37^＊＊, and r= -0.54^＊＊）, and positively associated with yield reduction percentage （r = 0.58^＊＊, and r= 0.58^＊＊） in 2003 and 2004, respectively. The DRI of genotypes was strongly associated with grain yield （r = 0.87^＊＊, and r= 0.77^＊＊）, fertility percentage （r = 0.66^＊＊ and r= 0.54^＊＊）, harvest index （r= 0.67^＊＊ and r= 0.61^＊＊）, and negatively associated with grain reduction percentage （r=-0.70^＊＊, and r=-0.73^＊＊） under drought stress. The results indicate that genotypes with drought resistance can be identified by measuring yield potential, delay in flowering, reduction in plant height, or DRI under test environments of well-watered and drought stress.

关键词： delay in heading date drought resistance drought response index Oryza sativa reduction in plant height yield potential.

Mechanism of apoptotic effects induced selectively by ursodeoxycholic acid on human hepatoma cell lines

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World Journal of Gastroenterology 2007年第11期13卷 1652-1658页

作者： Hui Liu Cheng-Yong Qin Guo-Qing han Hong-wei Xu mei Meng Zhen Yang Department of Gastroenterology Shandong Provincial Hospital Shandong University Jinan 250021 Shandong Province China

AIM: To investigate the effects of ursodeoxycholic acid (UDCA) on apoptosis and proliferation of hepatoma cell lines. METHODS: Human hepatoma cell lines HepG2 and BEL 7402 were cultured in medium supplemented with different concentrations of UDCA, normal human hepatic line L-02 was used as control. Cell proliferation, apoptosis and gene expression were detected using methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, Western blot, DNA ladder assay, electron microscopy, and immunocytochemistry. RESULTS: Ursodeoxycholic acid inhibited the proli- feration of HepG2 and BEL7402 cell lines in a dose- dependent manner. Ursodeoxycholic acid can change cell cycle distribution of HepG2 and BEL7402, the proportion of cells in G0-G1 phase increased whereas the proportion of S phase cells and G2-M phase cells decreased. Ursodeoxycholic acid arrested the cell cycle in G0-G1 phase by down-regulating the cell cycle related proteins cyclin D1, D3 and retinoblastoma protein (pRb). The apoptotic rates of HepG2 and BEL7402 treated with UDCA (1.0 mmol/L) were significantly higher than those of control. In the HepG2 and BEL7402 treated with UDCA, expression of bcl-2 decreased whereas expression of Bax increased, the nuclear fragmentation and chromosomal condensed, cells shrank and lost attachment, apoptotic bodies and DNA ladders appeared. UDCA had no effect in inducing apoptosis on L-02 cell lines. CONCLUSION: UDCA can selectively inhibit proliferation and induce apoptosis of HepG2 and BEL7402 cell lines by blocking cell cycle and regulating the expression of Bax/bcl-2 genes.

关键词： Liver neoplasm Ursodeoxycholic acid Apoptosis Mechanism Cell lines

Recombinant scFv Antibodies against E Protein and N Protein of Severe Acute Respiratory Syndrome Virus

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 541-547页

作者： Hui LIU , Yan-Li DING, wei han, mei-Yun LIU, Rui-Yang TIAN, Sheng-Li YANG, and Yi GONGShanghai Research Center of Biotechnology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences, Shanghai 200233, China Shanghai Research Center of Biotechnology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200233 China. huiliu@***

Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, Kd) of SARS-CoV E protein was 5.7×10–8 M for B10 and 8.9×10–8 M for C20. The affinity of A17 for N protein was 2.1×10–6 M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot.

关键词： SARS-CoV antibody scFv phage display affinity chromatography

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试述《米尔撒德》在中国的流传与翻译

回族研究 2017年第4期 92-99页

作者：魏寒梅陕西师范大学宗教研究中心陕西西安610021 新疆农业职业技术学院马克思主义学院新疆昌吉831100

成书于13世纪的苏非哲学著作《米尔撒德》,到了明末清初,受到中国伊斯兰经学大师的争相研究与翻译。从研究、翻译该书的经师身份和该书流传的区域看,它反映出当时苏非主义不仅在西北地区传播,而且也波及到华北、中原和西南等地区。《米... 详细信息

成书于13世纪的苏非哲学著作《米尔撒德》,到了明末清初,受到中国伊斯兰经学大师的争相研究与翻译。从研究、翻译该书的经师身份和该书流传的区域看,它反映出当时苏非主义不仅在西北地区传播,而且也波及到华北、中原和西南等地区。《米尔撒德》的流传和翻译有其特殊时代背景,从中可以观察苏非主义在中国传播的区域问题,同一思潮的传播在不同地区选择的路径不同,最终出现的结果差异性较大,此现象为当代伊斯兰教中国化提供了借鉴与思考。

关键词：《米尔撒德》翻译苏非主义传播影响

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基于粗糙集和D-S证据理论的交通拥堵预警

福州大学学报（自然科学版） 2008年第1期36卷 69-72页

作者：卫寒梅郑应文福州大学自动化所福建福州350002

鉴于拥堵预警中的信息和知识具有随机性和不确定性、采集到的数据又常常包含着噪声的特点,利用粗糙集能够对数据进行分析和处理,D-S理论能够分析各种不完备信息的特点,提出了一种算法——基于粗糙集和D-S理论,计算交通可能发生拥堵的概... 详细信息

鉴于拥堵预警中的信息和知识具有随机性和不确定性、采集到的数据又常常包含着噪声的特点,利用粗糙集能够对数据进行分析和处理,D-S理论能够分析各种不完备信息的特点,提出了一种算法——基于粗糙集和D-S理论,计算交通可能发生拥堵的概率,为交通拥堵预警提供分析与支持.仿真结果表明该方法是可行而且有效的.

关键词：交通拥堵预警粗糙集 D—S理论