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A review of isolation methods, structure features and bioactivities of polysaccharides from Dendrobium species

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Chinese Journal of Natural Medicines 2020年第1期18卷 1-27页

作者： yue han ZENG Hui DING Kan Glycochemistry and Glycobiology Lab Key Laboratoty of Receptor ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China

Dendrobium,orchid,is a traditional Chinese herb medicine applied extensively as tonic and precious food for thousands of years recorded in ancient Chinese medical book"Shen Nong’s Materia Medica".It’s well known that bioactivities are usually related to the ingredients’*** on the previous research,Dendrobium species contain amino acid,sesquiterpenoids,alkaloids and *** the bioactive substances,carbohydrate shows extensive activities in antitumor,antiglycation,immune-enhancing,antivirus,antioxidant,antitumor and ***,as the main biologically active substance,the exact structures and latent activities of polysaccharides from Dendrobium species are widely focused *** this review,we focus on the advancements of extraction methods and diversity of structures and bioactivities of polysaccharides obtained from Dendrobium species.

关键词： Dendrobium Polysaccharides Bioactivities Structures

Analysis of Ship Motion in An Ice Ridge Field Based on Empirical Approach

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China Ocean Engineering 2023年第6期37卷 912-922页

作者： SONG Ming YUAN Wei LIU Kun han yue School of Naval Architecture and Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China

The estimation of ship speed in ice ridge fields is important for both route planning and prediction of emergency response *** analytical method for estimating ship motion in first-year ice ridge is developed based on ice resistance models and ship motion equations,in which the effect of ship speed on ridge resistance is taken into *** model tests in level ice and one model test in ice ridge for an icebreaking multipurpose vessel are used to validate and benchmark the presented *** predicted results including level ice resistances,net thrust and ship motion in the ice ridge field are compared with the model test *** comparisons show that the presented method can generate reasonable *** effects of input parameters on ship speed,penetration depth and number of necessary rams to transit ridge have been *** on the calibrated model,insights into the ice resistance and the ship motion are *** is found that the energy consumption of the keel obtained by integral calculation of the keel resistance at the penetration distance is with the same magnitude as the result of the maximum keel resistance multiplied by the ridge *** addition,the effect of ridge width and keel depth on keel resistance and average transit speed is investigated.

关键词： ice ridge ice resistance model tests average transit speed

Research on theoretical and numerical methods of single bubble oscillation

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Journal of Hydrodynamics 2021年第4期33卷 872-877页

作者： Jie-min Zhan yue-han Chen Yu-tian Li Department of Applied Mechanics and Engineering Sun Yat-sen UniversityGuangzhou510275China

A comparison of theoretical investigation and numerical simulation of a single bubble oscillation is carried out in this *** theoretical research is based on solving Rayleigh-Plesset(R-P)equation and Keller-Miksis(K-M)equation using Runge-Kutta *** numerical method focuses on the two discrete methods in the volume of fluid(VOF)method,geometric reconstruction(GR)and modified high resolution interface capturing(MHRIC).The results show that the interface captured by MHRIC in the collapse stage is more stable,and the evolution of bubble radius agrees better with the theoretical *** R-P equation and K-M equation,which omit the effect of the energy equation,have limitations when the bubble collapses under ultra-high pressure.

关键词： Cavitation bubble dynamics volume of fluid method geometric reconstruction(GR)

Resolving rupture processes of great earthquakes: Reviews and perspective from fast response to joint inversion

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Science China Earth Sciences 2020年第4期63卷 492-511页

作者： han yue Yong ZhanG Zengxi GE Teng WANG Li ZHAO School of Earth and Space Sciences Peking UniversityBeijing 200871China

Resolving rupture processes of great earthquakes has fundamental importance to the study of earthquake physics,rupture dynamics, fault zone structure, and evolving processes. It also plays an essential role in earthquake hazard estimation,emergency response and seismic hazard mitigation. This paper reviews the major progress of the earthquake rupture process studies in the last decades, with an emphasize on the research directions of the department geophysics of Peking University including real-time response, back-projection techniques, geodetic data analysis, joint inversion and inversion in complex earth medium. We discussed the advantages and limitations of tradition methods;proposed a systematic and integrated approach from fast-response to detailed study. We also raised perspectives of using source models for ground motion prediction and the possibility of full-dynamic inversion.

关键词： Finite fault model Backprojection Joint inversion Strong motion Simulation

A system and analytical method for testing the electrical parameters of soil

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Transportation Safety and Environment 2021年第1期3卷 57-71页

作者： yue han Ailan Che Shaokong Feng School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai200030China

The electrical parameters of the rock and soil are closely related to the physical *** measuring the resistivity and polarizability of rock and soil reasonably,information on physical parameters such as moisture content,pore ratio and compaction degree can be obtained.A system for testing the electrical parameters of soil samples based on the middle step quadrupole method is developed in this *** system uses a series of copper needles as the electrodes of the soil-mounted test tube,and uses a multi-point multi-layer test method for layered data acquisition,which can solve the problems caused by the capacitance phenomenon and the heterogeneity of the soil *** the same time,factors affecting the test results,such as uneven soil preparation and water addition and electrode disturbance,are regarded as noise.A data-analysis method based on median filtering is proposed to process and analyse the test results,and the relationships between resistivity,polarizability,moisture content and compaction degree of the sample are *** results show that the resistivity decreases nonlinearly as moisture content increases,and the correlation with compaction is not strong;the polarizability increases as moisture content increases with fluctuation,and it first increases and then decreases with an increase in compaction.

关键词： testing system for electrical parameters median filtering electrical resistivity polarizability moisture content compaction degree

大鼠脂肪间充质干细胞及其外泌体的蛋白组学对比分析

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中国组织工程研究 2025年第13期29卷 2683-2689页

作者：张鑫瑞韩悦雷蕾刘建宇耿承奎云南中医药大学第二临床医学院云南中医药大学呈贡校区云南省昆明市650000 昆明医科大学附属延安医院骨科云南省昆明市650000

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外... 详细信息

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外泌体并进行鉴定;运用DIA蛋白组学分析大鼠脂肪间充质干细胞及其外泌体差异表达蛋白,对差异表达蛋白进行GO和KEGG分析。结果与结论:①脂肪间充质干细胞以长梭形为主,类似成纤维样;②经BCA法测定外泌体悬液的蛋白质量浓度为7.66 mg/mL;③外泌体呈典型的茶杯型,可见双层膜的囊泡结构,中央为低电子密度成分,外泌体粒径分布峰值为112.2 nm,浓度为7.5×10^(11)粒子/mL,平均直径在70-140 nm之间;④外泌体有表面标记蛋白CD9、TSG101高表达;⑤GO分析脂肪间充质干细胞及其外泌体的差异表达蛋白主要集中在细胞外基质和突触等位置,与离子结合和核糖体的结合能力相关,在细胞黏附和翻译过程中最为显著;⑥KEGG通路分析结果显示,差异表达蛋白主要参与细胞外基质受体交互、核糖体和细胞因子受体相互作用等,并且与胆固醇和甲状腺等多种代谢性疾病有关。

关键词：脂肪间充质干细胞外泌体蛋白组学对比分析组织修复

Various infection status and molecular evidence for horizontal transmission and recombination of Wolbachia and Cardinium among rice planthoppers and related species

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Insect Science 2013年第3期20卷 329-344页

作者： Kai-Jun Zhang Xiao han Xiao-yue Hong Department of Entomology Nanjing Agricultural University Nanjing China

Wolbachia and Cardinium are widely distributed and are considered important for their ability to disturb reproduction and affect other fitness-related traits of their hosts. By using multilocus sequence typing （MLST）, RFLP （restriction fragment length poly- morphism） and 16S ribosomal DNA gene sequencing methods, we extensively surveyed Wolbachia and Cardinium infection status of four predominant rice planthoppers and one kind of leafhopper in different rice fields. The results demonstrated that Sogatellafurcifera （Horv^ith） and Laodelphax striatellus （Fallrn） were infected with the same Wolbachia strain （wStri）, while Nilaparvata lugens （St^l） and its closely related species Nilaparvata muiri China were infected with two phylogeneticlly distant strains, wLug and wMui, respectively. Three new Wolbachia strains （provisionally named wMfasl, wMfas2 and wMfas3） were detected in the leafhopper Macrostelesfascifrons （Stal）. Only S. furcifera was co-infected with Cardinium, which indicated that the distribution of Cardinium in these rice planthop- pers was narrower than that of Wolbachia. Unambiguous intragenic recombination events among these Wolbachia strains and incongruent phylogenetic relationships show that the connections between different Wolbachia strains and hosts were more complex than we expected. These results suggest that horizontal transmission and host associated special- ization are two factors affecting Wolbachia and Cardinium infections among planthoppers and their related species.

关键词： Cardinium infection status intragenic recombination rice planthopper,Wolbachia

Mechanobiology in vascular remodeling

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National Science Review 2018年第6期5卷 933-946页

作者： yue han Kai Huang Qing-Ping Yao Zong-Lai Jiang Institute of Mechanobiology & Medical Engineering School of Life Sciences & BiotechnologyShanghai Jiao Tong University School of Biological Science & Medical Engineering Beijing Advanced Innovation Center for Biomedical Engineering Beihang University

Vascular remodeling is a common pathological process in cardiovascular diseases and includes changes in cell proliferation, apoptosis and differentiation as well as vascular homeostasis. Mechanical stresses, such as shear stress and cyclic stretch, play an important role in vascular remodeling. Vascular cells can sense the mechanical factors through cell membrane proteins, cytoskeletons and nuclear envelope proteins to initiate mechanotransduction, which involves intercellular signaling, gene expression, and protein expression to result in functional regulations. Non-coding RNAs, including micro RNAs and long non-coding RNAs, are involved in the regulation of vascular remodeling processes. Mechanotransduction triggers a cascade reaction process through a complicated signaling network in cells. High-throughput technologies in combination with functional studies targeting some key hubs and bridging nodes of the network can enable the prioritization of potential targets for subsequent investigations of clinical translation. Vascular mechanobiology, as a new frontier field of biomechanics, searches for principles of stress-growth in vasculature to elucidate how mechanical factors induce biological effects that lead to vascular remodeling,with the goal of understanding the mechanical basis of the pathological mechanism of cardiovascular diseases at the cellular and molecular levels. Vascular mechanobiology will play a unique role in solving the key scientific problems of human physiology and disease, as well as generating important theoretical and clinical results.

关键词： mechanobiology vascular remodeling cardiovascular disease mechanotransduction endothelial cell vascular smooth muscle cell nuclear envelope microRNA

Effect of Cr Content on the Compression Properties and Abrasive Wear Behavior of the High-Volume Fraction(TiC–TiB_2)/Cu Composites

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Acta Metallurgica Sinica(English Letters) 2014年第5期27卷 951-956页

作者： Feng Qiu yue han Ai Cheng Jianbang Lu Qichuan Jiang Key Laboratory of Automobile Materials Ministry of EducationDepartment of Materials Science and EngineeringJilin University

The （TiC-TiB2）/Cu composites with 50 vol% TiC-TiB2 ceramic particles were successfully fabricated by the combustion synthesis and hot press consolidation in a Cu-Ti-B4C-Cr system. The effects of the Cr content on the microstructures, hardness, compression properties, and abrasive wear behaviors of the composites were investigated. The final products consist of only Cu, TiC, and TiB2 phases, and the ceramic particles are distributed uniformly in these composites. The size of the ceramic particles decreases with Cr addition. As the Cr content increases, the yield strength, ultimate compression strength, microhardness, and abrasive wear resistance of the composites increase, and the fracture strain decreases.

关键词： Metal matrix composites Powder metallurgy Combustion synthesis Abrasive wear