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Effects of a fermented buckwheat flower and leaf extract on the blood glucose and lipid profile of type 2 diabetic db/db mice

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Journal of Traditional Chinese Medicine 2020年第2期40卷 197-203页

作者： Wang Jianxing Yu Xiaohan Jiang Yan Wang Yan Li Ying han shuying North China University of Science and Technology Jitang CollegeTangshan 063000China North China University of Science and Technology Clinical Medical CollegeTangshan 063000China North China University of Science and Technology Basic Medical CollegeTangshan 063000China Hebei Key Laboratory on Chronic Diseases Tangshan 063000China Tangshan Basic Clinical Research Key Laboratory on Chronic Diseases Tangshan 063000China

OBJECTIVE: To determine the efficacy of a fermented buckwheat flower and leaf extract(EFBFL) for the reduction of blood glucose and lipid dysregulation in spontaneously obese type 2 diabetic db/db mice, and to explore the possible mechanisms ***: Forty 9-week-old male db/db mice were randomly allocated to a high-dose EFBFL group(EFBFL-H), a low-dose EFBFL group(EFBFL-L),a metformin hydrochloride positive control group(MEG), and a db/db control group(MG), and there was also a db/m negative control group(NCG)(n =10). Oral glucose tolerance tests(OGTT) were performed after 7 weeks of treatment. At the end of 8 weeks of treatment, random blood glucose(RBG),glycosylated hemoglobin(Hb Alc), fasting plasma glucose(FPG), fasting serum insulin(FINS), triglyceride(TG), serum total cholesterol(TC), free fatty acids(FFA), high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol(LDL-c) were measured, the homeostasis model assessment-insulin resistance(HOMA-IR) was calculated. Immunohistochemistry and western blotting measured the expression of glucose transporter4(GLUT4) and peroxisome proliferator-activated receptor γ(PPAR-γ) by in skeletal ***: The MG mice had a significantly increased in RBG, Hb Alc, the HOMA-IR level, the serum of TG, TC, LDL-c, but a decreased in glucose tolerance and the protein expression of GLUT4 and PPAR-γ compared with the NCG. Compared with the MG, EFBFL groups significantly decreased RBG, Hb Alc, and the HOMA-IR level, increased glucose tolerance. Meanwhile EFBFL groups reduced the serum TG, TC, and LDL-c in a dose-dependent manner. In addition, EFBFL increased the protein expression of GLUT4 and PPAR-γ in the skeletal muscle of db/db *** was significant difference between the MG group and EFBFL ***: These findings suggest that EFBFL has anti-diabetic effects in db/db mice, ameliorating glucose intolerance, lipid dysregulation, and insulin resistance.

关键词： Eriogonum Diabetes mellitus,type 2 Muscle,skeletal Glucose transporter type 4 PPAR gamma

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Novel computer-assisted approach to quick prediction and optimization of gradient separation for online enrichment-reversed phase liquid chromatography tandem system

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Chinese Chemical Letters 2023年第9期34卷 280-283页

作者： shuying han Yilin Song Xinyi Jiang Junqin Qiao An Kang Haishan Deng Dong Zhu Rui Liu Hongzhen Lian College of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry&Chemical Engineering and Center of Materials AnalysisNanjing UniversityNanjing 210023China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative MedicineNanjing 210023China

An algorithm capable of predicting and optimizing the gradient separation of LC×LC system was developed in this *** groups of structural analogues,five ginsenosides as well as eight bisphenols,which were difficult to discriminate in routine analysis,were used to verify the effectiveness of the proposed algorithm in fast separation *** errors of retention times below 1%were found in the retention prediction for all types of gradient programs,implying that the theory could lead to high quality in prediction of the retention times under gradients ***,84%of relative average deviations(RADs)between the predicted peak width and the measured ones were less than 20%.The larger deviation occurred at the time when the peak appeared while the gradient of the mobile phase changed,which led the deviations increased to 20%–42%.In all,method development and optimization for LC×LC tandem system was realized by the homemade user-friendly *** present protocol may turn on great opportunities for the convenient method development in analysis of trace structural analogues in environmental,food and biological samples.

关键词： LC×LC tandem system Separation prediction Gradient elution Peak shape Separation optimization algorithm

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A Case Study of Oral English Teaching for College EFL Learners in China： Problems and Implications

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International English Education Research 2014年第5期 92-95页

作者： Jing han shuying han handan College HeBei Handan 056005

Oral English teaching is not only an integral part of college English language teaching but also a crucial factor related to whether the language teaching goals can be achieved or not. This research has investigated the current situations and existing problems concerning oral English teaching on the basis of a case study of the authentic oral English classroom teaching at a city college in China. The findings and solutions may provide some implications for oral English language teaching at the college level in China.

关键词： local college English majors teaching reform exploration and practice

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Mechanism and Application Effect of Bacillus licheniformis on Poultry

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Animal Husbandry and Feed Science 2015年第2期7卷 111-112,115页

作者： Wang Changqing han shuying Jiangsu Dongtai Agricultural Committee Jiangsu Dongtai Animal Husbandry and Veterinary Station

Bacillus licheniformis has the biological characteristics of strong resistance to stress, high temperature, high pressure, pH and bile salt, which also has unique advantage in application safety, antibacterial activity and stability. The recent research results on mechanism of B. licheniformis and its application effect in poultry production are elaborated in the paper.

关键词： Bacillus licheniformis Poultry Mechanism Application effect

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Ecological Scale Breeding Technique of Local Chickens

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Animal Husbandry and Feed Science 2015年第5期7卷 261-263,291页

作者： Xu Guoqing Wang Changqing han shuying Dongtai Municipal Animal Husbandry and Veterinary Station Dongtai Institute of Agricultural Science Dongtai Municipal Agriculture Committee

Developing ecological scale breeding of local chickens with natural conditions, such as forest lands, grass lands, orchards and mulberry fields, not only can improve the quality of poultry products and the production benefits of chicken breeding, but also can develop and use local chicken germplasm effectively and promote their breed protection and industrialization. From integration and application of breed selection, breeding management, nutrient regulation, grass planting and grazing, a new ecological scale breeding technique of local chickens is proposed.

关键词： Local chickens Ecological Scale Breeding Technique

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栗黑层孔菌产三萜条件优化与提取工艺

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林业科学 2016年第9期52卷 147-153页

作者：韩树英池玉杰东北林业大学林学院哈尔滨150040

【目的】研究菌株CY-2012菌丝体内三萜类物质的产生条件,以期得到最高量的三萜类物质。【方法】以28℃,150 r·min^(-1)作为初始培养条件,用基础培养基对采自长白山的栗黑层孔菌菌株CY-2012进行培养,然后采用2项单因子试验、1次4因素3... 详细信息

【目的】研究菌株CY-2012菌丝体内三萜类物质的产生条件,以期得到最高量的三萜类物质。【方法】以28℃,150 r·min^(-1)作为初始培养条件,用基础培养基对采自长白山的栗黑层孔菌菌株CY-2012进行培养,然后采用2项单因子试验、1次4因素3水平的正交试验,对该菌株产三萜类物质的液体培养基成分进行优化;在此基础上,应用异丙醇和硫酸铵双水相萃取技术提取菌丝体内的三萜,采用2项单因子试验、1次3因素3水平的正交试验,对培养基成分优化后获得的菌丝体进行三萜类物质提取工艺研究。【结果】菌株CY-2012产三萜最优的培养基成分组合是:去皮马铃薯200 g·L^(-1)、葡萄糖25.0 g·L^(-1)、乳糖3.0 g·L^(-1)、酵母15.0 g·L^(-1)、MgSO_4·7H_2O 0.5 g·L^(-1)、KH_2PO_40.5 g·L^(-1)、NH_4NO_30.3 g·L^(-1)、维生素B10.5 g·L^(-1),在28℃、150 r·min-1条件下培养10天菌丝干质量达0.769 g·(100 m L)-1,其中三萜类物质产量高达14.529 mg·(100 m L)-1,分别是初始培养条件下的9.96和35.52倍。双水相萃取技术最佳的提取工艺为异丙醇体积分数40%,硫酸铵质量浓度0.18 g·mL^(-1),室温浸提2 h,超声时间35 min,得到的三萜提取率为3.65%。【结论】通过优化培养基成分可以较大幅度地提高三萜的产量,优化异丙醇和硫酸铵双水相萃取提取工艺可以提高三萜提取率。

关键词：栗黑层孔菌三萜化合物优化双水相体系提取工艺

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Natural plant-derived 3,4-dihydroxybenzaldehyde ameliorates reproductive damage of male mice

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Journal of Chinese Pharmaceutical Sciences 2022年第6期31卷 429-440页

作者： Hongning Zhang shuying han Xiaoping Pu Chunna An Department of Pharmacology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China Department of Pharmacology School of Basic Medical SciencesNorth China University of Science and TechnologyTangshan 063210China Department of Molecular and Cellular Pharmacology School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

In the present study,we studied the effects of 3,4-dihydroxybenzaldehyde,derived from Salvia miltiorrhiza Bunge,on mouse sperm survival and sperm membrane integrity in vitro and on mouse reproductive damage induced by cyclophosphamide in vivo.3,4-Dihydroxybenzaldehyde(0.01,0.1,and 1 mg/m L)improved sperm survival rate and sperm membrane integrity in separated mouse sperm(all Phanced the expressions of VCAM-1,PEDF,VEGF,and PPARγ.These findings indicated that 3,4-dihydroxybenzaldehyde increased mouse sperm survival and sperm membrane integrity in vitro and reduced mouse reproductive damage induced by cyclophosphamide via DJ-1 and other targets in vivo.3,4-Dihydroxybenzaldehyde might thus be useful for treating male reproductive damage and antineoplastic cyclophosphamide-induced reproductive toxicity.

关键词： Natural plants 3 4-Dihydroxybenzaldehyde Reproductive damage

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Molecular mechanism of adjuvant effect mediated by polysaccharide from Physalis alkekengi L.

Molecular mechanism of adjuvant effect mediated by polysacch...

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第十届全国免疫学学术大会

作者： Fan Yang Hao Sun shuying han Guiyun Wang School of Life Sciences Northeast Normal University

Background:We have evaluated adjuvant effect of polysaccharide(PPSB)isolated from the fruits of Physalis alkekengi *** DNA vaccine,and analyzed the structure of *** results indicated that PPSB significantly enhanced Th1and Th2 *** importantly,it was found that the mice immunized with DNA vaccine combined with PPSB not only had fewer CFU(colony forming unites)in the kidneys than mice immunized with DNA vaccine,but also a statistically significant higher survival rate over PBS-injected group when the immunized mice were challenged with living ***,it is unclear about the molecular mechanisms of PPSB used as an ***,we focus on the molecular mechanisms of PPSB adjuvant effect in this ***:We investigated the stimulating effect of PPSB on phenotypic and functional maturation of dendritic cells(DCs).Phenotypic maturation was confirmed by expressions of CD80 and CD86;endocytosis was evaluated by analysis nonspecific binding of FITC-dextran to *** activation was demonstrated by cytokine production of MIP-1α、IL-12、*** role of toll-like receptor 4(TLR4)as a membrane receptor of PPSB in DCs was elucidated by binding of PPSB to DCs in using of anti-MD-2/TLR4 to block TLR4 in ***:PPSB induced the phenotypic maturation of DCs,and decreased the phagocytic *** was also found PPSB increased the cytokine production of MIP-1α、IL-12、*** importantly,it was shown using anti-MD-2/TLR4 impaired the binding of PPSB to ***:The results indicate PPSB induces the maturation and function of DCs,and enhances the cytokine production of ***4 of DCs may be the one of membrane receptors of PPSB.

关键词： Physalis alkekengi L. DCs Adjuvant Polysaccharide

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神经细胞凋亡与胞浆钙离子

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中风与神经疾病杂志 1999年第3期16卷 131-132页

作者：韩恩吉韩淑英济南山东医科大学附属医院神经内科山东省淄博市周村第二人民医院

目的应用钙载体和ＰＣ１２细胞株探讨胞浆钙离子与神经细胞凋亡之间的关系。方法水母发光蛋白导入法测量ＰＣ１２胞浆钙离子，细胞存活率监测细胞死亡，ＤＮＡ琼脂糖电泳证实细胞凋亡。结果钙载体（Ａ２３１８７）升高ＰＣ１２胞浆钙离... 详细信息

目的应用钙载体和ＰＣ１２细胞株探讨胞浆钙离子与神经细胞凋亡之间的关系。方法水母发光蛋白导入法测量ＰＣ１２胞浆钙离子，细胞存活率监测细胞死亡，ＤＮＡ琼脂糖电泳证实细胞凋亡。结果钙载体（Ａ２３１８７）升高ＰＣ１２胞浆钙离子，降低细胞存活率，ＤＮＡ琼脂糖电泳显示典型的ＤＮＡ梯形带。结论胞浆钙离子升高激活内源性核酸内切酶，导致ＤＮＡ核小体间断裂，启动神经细胞凋亡。

关键词：胞浆钙离子细胞凋亡核酸内切酶神经细胞

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乳白耙齿菌菌株CB1对刚果红染料的脱色条件

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林业科学 2016年第8期52卷 115-121页

作者：石亚攀池玉杰于存韩树英东北林业大学林学院哈尔滨150040 贵州大学林学院贵阳550025

【目的】对从自然界得到的1株乳白耙齿菌菌株CB1(简称Il-CB1)进行系统发育分析与鉴定,并研究其对刚果红染料的脱色条件,以期为乳白耙齿菌在染料废水脱色中的应用提供依据。【方法】在形态学鉴定的基础上,克隆菌株Il-CB1的ITS序列并进行... 详细信息

【目的】对从自然界得到的1株乳白耙齿菌菌株CB1(简称Il-CB1)进行系统发育分析与鉴定,并研究其对刚果红染料的脱色条件,以期为乳白耙齿菌在染料废水脱色中的应用提供依据。【方法】在形态学鉴定的基础上,克隆菌株Il-CB1的ITS序列并进行系统发育分析。采用单因素方法检测p H值、刚果红浓度、染料加入时间对Il-CB1脱色率的影响;基于正交试验检测碳源浓度、氮源浓度、Cu2+浓度、Mn2+浓度、接种量5个因素对Il-CB1脱色率的影响。【结果】菌株I1-CB1的ITS序列与其他不同来源的24个乳白耙齿菌的ITS序列归为一类,从而在分子水平上鉴定其为乳白耙齿菌。p H值对Il-CB1脱色刚果红的影响:脱色率随p H值增加先升高后降低,p H=3和8时脱色率均较低,其脱色刚果红的最适p H=6.0。刚果红浓度对Il-CB1脱色的影响:在液体条件下的脱色率随刚果红浓度的升高呈先升高后降低的趋势,对100 mg·L-1的刚果红脱色率最高,当继续升高染料浓度为250 mg·L-1时,脱色率明显下降,表明较低浓度的刚果红促进Il-CB1对其脱色,而高浓度的刚果红对Il-CB1脱色产生抑制。正交试验表明,Il-CB1对刚果红脱色的最优因素组合为:葡萄糖10 g·L-1、蛋白胨3 g·L-1、Cu2+和Mn2+的添加浓度均为0.2 mmol·L-1、在250 m L三角瓶中装液量为100 m L的条件下接种量为7 m L菌丝母液,在以上条件下第5天时染料脱色率高达99.60%。在上述所有培养条件优化的基础上,染料加入时间对Il-CB1脱色刚果红的影响:与0 h加入刚果红染料相比,在菌株培养3天后加入刚果红染料,其脱色时间明显缩短,初始脱色率就处于较高水平,在菌株培养3天时加入刚果红染料处理5天后的脱色率高达99.99%。【结论】菌株Il-CB1为乳白耙齿菌,其可有效降解刚果红染料,在染料废水降解中具有重要的应用潜能。

关键词：乳白耙齿菌菌株CB1 ITS序列刚果红脱色

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