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Study on the Extraction and Purification of Microcystins （MC-LR）

Study on the Extraction and Purification of Microcystins （M...

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2010 3rd International Conference on Future BioMedical Information Engineering (FBIE 2010)

作者： Zhang Wenyi a han ningb Yao Lirong a Qiu Xiaolan a and Chen Xiaoliangb a School of Environmental and Safety Engineering ChangZhou University Changzhou City Jiangsu Province 213164 China b Department of Equipment ManufactureJiangsu Hengliang Centrifuge Manufacturing Co. LTD Jintan City Jiangsu Province 213200 China

The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.

关键词： Microcystins (MC-LR) Extraction Purification HPLC

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Study on Biological Aerated Filter (BAF) of Algae-contained Micropolluted Water

Study on Biological Aerated Filter (BAF) of Algae-contained ...

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2010 3rd International Conference on Future BioMedical Information Engineering (FBIE 2010)

作者： Zhang Wenyi a, han ningb, Yan Gang a, Xu Xia a and Chen Xiaoliangb a, School of Environmental and Safety Engineering ChangZhou University, Changzhou City, Jiangsu Province, 213164, China b, Department of Equipment ManufactureJiangsu Hengliang Centrifuge Manufacturing Co., LTD , Jintan City, Jiangsu Province, 213200, China

Using NaCl modified zeolite with high specific surface area as filter media, algae-contained micropolluted water was treated with biological aerated filter (BAF) process. The removal efficiencies of the process on micro-pollutants and removal mechanism of algae-contained micropolluted water were investigated and studied. The results showed that better removal efficiency was obtained in turbidity, Chlorophyll-a, UV254, and CODMn when algae-contained micropolluted water was treated by BAF. The optimal process parameters of the treatment of algae-contained micropolluted water with BAF were gases/water ratio of 0.5.5, backwash cycle of 3 d, air/water backwash strength of 5 L/(m2·s), air backwash strength of 137 L/(m2·s), backwash time of 150min, temperature of 250℃, pH value of 7.8.5 and hydraulic load of 0.23.35 m3/(m2·h). The results of continuous running for 21 days showed that the removal efficiency of turbidity, Chlorophyll-a, UV254, and CODMn were up to 71.61%, 81.35%, 28.34% and 32.97% respectively under the conditions of these optimal parameters, and the effluent water could meet Grade II of the Drinking Water Quality Standards (CJ 3020～1993). This study could provide technical information for town water plant reconstruction of Taihu Lake and Chaohu Lake basin.

关键词： Algae-contained Micropolluted Water Biological Aerated Filter (BAF) Chlorophyll-a Microcystins UV254

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