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A nonsensory odorant-binding protein plays an important role in the larval development and adult mating of Spodoptera frugiperda

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Insect Science 2023年第5期30卷 1325-1336页

作者： Wei-kang han Feng-Xian Tang Na Yu Yi-Xi Zhang Ze-Wen Liu Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education) College of Plant ProtectionNanjing Agricultural UniversityNanjingChina

Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals in insects. Several OBPs in insect olfactory systems have been functionally characterized, and they provide excellent targets for pest control. The functions of some OBPs that are highly expressed in the nonsensory organs of insects remain unclear. Here, the physiological function of an OBP (OBP27) that was highly expressed in the nonsensory organs of Spodoptera frugiperda was studied. OBP27 was nested within the Plus-C cluster according to phylogenetic analysis. The transcription of OBP27 steadily increased throughout the development of S. frugiperda, and transcripts of this gene were abundant in the fat body and male reproductive organs. An OBP27 knockout strain with an early frameshift mutation was obtained using the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9 (Cas9) system. The development time of OBP27^(−/−) larvae was significantly longer than that of other larvae. Both male and female OBP27^(−/−) pupae weighed significantly less than wild-type (WT) pupae. In crosses of OBP27^(−/−) males or females, the mating rate was lower and the mating duration was longer for OBP27^(−/−) male–WT female pairs than for WT–WT pairs. By contrast, the mating rate, hatching rate, and number of eggs of OBP27^(−/−) female–WT male pairs and WT–WT pairs were similar. These findings indicate that OBP27 plays an important role in the larval development and mating process in male adults. Generally, our findings provide new insights into the physiological roles of nonsensory OBPs.

关键词： CRISPR/Cas9 nonsensory OBP reproduction Spodoptera frugiperda

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Brain endothelial cell-derived extracellular vesicles with a mitochondria-targeting photosensitizer effectively treat glioblastoma by hijacking the blood-brain barrier

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药学学报:英文版 2023年第9期13卷 3834-3848页

作者： Thuy Giang Nguyen Cao Ji Hee kang Su Jin kang Quan Truong Hoang han Chang kang Won Jong Rhee Yu Shrike Zhang Young Tag Ko Min Suk Shim Division of Bioengineering Incheon National UniversityIncheon 22012Republic of Korea College of Pharmacy Gachon UniversityIncheon 21936Republic of Korea Department of Pharmacy Integrated Research Institute of Pharmaceutical Sciencesand BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future PharmacyCollege of Pharmacythe Catholic University of KoreaGyeonggi-do 14662Republic of Korea Research Center for Bio Materials&Process Development Incheon National UniversityIncheon 22012Republic of Korea Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMA 02139USA

Glioblastoma(GBM)is the most aggressive malignant brain tumor and has a high mortality *** therapy(PDT)has emerged as a promising approach for the treatment of malignant brain ***,the use of PDT for the treatment of GBM has been limited by its low blood-brain barrier(BBB)permeability and lack of cancer-targeting ***,brain endothelial cell-derived extracellular vesicles(bEVs)were used as a biocompatible nanoplatform to transport photosensitizers into brain tumors across the *** enhance PDT efficacy,the photosensitizer chlorin e6(Ce6)was linked to mitochondria-targeting triphenylphosphonium(TPP)and entrapped into *** Ce6(TPP-Ce6)selectively accumulated in the mitochondria,which rendered brain tumor cells more susceptible to reactive oxygen species-induced apoptosis under light ***,the encapsulation of TPP-Ce6 into b EVs markedly improved the aqueous stability and cellular internalization of TPP-Ce6,leading to significantly enhanced PDT efficacy in U87MG GBM *** in vivo biodistribution study using orthotopic GBM-xenografted mice showed that b EVs containing TPP-Ce6[b EV(TPP-Ce6)]substantially accumulated in brain tumors after BBB penetration via transferrin receptor-mediated *** such,b EV(TPP-Ce6)-mediated PDT considerably inhibited the growth of GBM without causing adverse systemic toxicity,suggesting that mitochondria are an effective target for photodynamic GBM therapy.

关键词： Extracellular vesicle Chlorin e6 Triphenylphosphonium Mitochondria-targeting photosensitizer Photodynamic therapy Blood-brain barrier Glioblastoma Transferrin receptor

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纳米碳材料在水泥基材料中分散技术及表征方法的研究进展

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硅酸盐通报 2024年第5期43卷 1796-1811页

作者：李雨洋赵立革刘爽韩康刘艳军李润丰郑永超北京建筑材料科学研究总院有限公司北京100041 固废资源化利用与节能建材国家重点实验室北京100041 金隅住宅产业化(唐山)有限公司唐山064108

纳米碳材料具有独特的结构和优异的性能,能够作为增强材料改善水泥基材料的性能,受到了广泛研究与关注,然而这均以纳米碳材料在水泥中能够良好分散为基础。纳米碳材料比表面积大,分子间作用力强,难以直接分散在水泥基体中,且分散效果也... 详细信息

纳米碳材料具有独特的结构和优异的性能,能够作为增强材料改善水泥基材料的性能,受到了广泛研究与关注,然而这均以纳米碳材料在水泥中能够良好分散为基础。纳米碳材料比表面积大,分子间作用力强,难以直接分散在水泥基体中,且分散效果也不易判断。本文系统梳理了常见的纳米碳材料在水泥基材料中团聚的机理,总结了近年来国内外学者采用的分散技术,详细分析了各种分散方法的机理与优缺点,同时列举了纳米碳材料在水泥基材料中分散效果的表征方法。在未来的研究中,可以重点开发更高效、更适合工业化生产的分散方法,研究纳米碳材料在水泥基体中真实分散状态的评价手段,助力纳米碳改性水泥基复合材料在实际工程项目中的规模化应用。

关键词：纳米碳材料水泥基材料团聚分散改性表征方法

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基于动态共价键类玻璃高分子材料的制备及应用研究进展

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塑料科技 2024年第6期52卷 155-160页

作者：康菡子朱浩霖周文欣杨柳张浩宇刘晓莉陈瑨河南省科学院化学研究所有限公司河南郑州450002 郑州市管城回族区第六中学河南郑州450048

热固性材料因性能卓越而广泛应用,但难重塑导致资源浪费和环境污染。将动态共价键引入聚合物的交联网络中,可制备兼具热固性材料性能并可重复回收利用的类玻璃高分子材料。文章总结了不同共价键交换类型类玻璃高分子的合成特点,着重阐... 详细信息

热固性材料因性能卓越而广泛应用,但难重塑导致资源浪费和环境污染。将动态共价键引入聚合物的交联网络中,可制备兼具热固性材料性能并可重复回收利用的类玻璃高分子材料。文章总结了不同共价键交换类型类玻璃高分子的合成特点,着重阐述了酯交换、二硫交换、亚胺交换的反应机理及优缺点。针对类玻璃高分子的研究现状,分析了其在自修复、可焊接等领域的应用前景,最后结合目前市场需求及材料性能对未来类玻璃高分子的发展前景作出展望。

关键词：类玻璃高分子动态共价键自修复可焊接

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CFD-DEM study on the synergistic effect of coke consumption and particle dynamics in the blast furnace raceway

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Particuology 2024年第6期89卷 270-278页

作者： Peng Xu Peng han Zhijun He Xizhong An kang Sun Lihua Gao Key Laboratory of Green Low-Carbon and Intelligent Metallurgy School of Materials and MetallurgyUniversity of Science and Technology LiaoningAnshan 114000China Key Laboratory for Ecological Metallurgy of Multimetallic Mineral of Ministry of Education School of MetallurgyNortheastern UniversityShenyang110819China

In this study,the gas-solid flow process in the blast furnace raceway is numerically simulated using coupled computational fluid dynamics and the discrete element method(CFD-DEM).The coke reaction kinetics data are imported into the DEM model to reproduce the consumption process of each coke *** effects of inlet gas velocity and angle on the morphology of the raceway,coke consumption rate,coke bed temperature,and particle size distribution in the blast process are systematically investigated and *** results show that the consumption of coke particles promotes the formation of raceways during the blast *** the same time,a coke mixture layer is produced at the edge of the *** higher the inlet gas velocity,the thicker the coke mixture layer in the middle and upper parts of the raceway region,and the larger the proportion of small particles in the coke mixture *** effect of the inlet gas angle on the raceway region is less than the inlet gas ***,with the increase in the inlet gas angle,the high-temperature region of the coke bed extends downward gradually,which is conducive to activating the hearth.

关键词： Blast furnace Raceway Multiphase flow CFD-DEM Modeling

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Continuous dimethyl carbonate synthesis from CO_(2) and methanol over Bi_(x)Ce_(1-x)O_(δ) monoliths:Effect of bismuth doping on population of oxygen vacancies,activity,and reaction pathway

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Nano Research 2022年第2期15卷 1366-1374页

作者： Yongdong Chen Yue Li Wei Chen Wen Wu Xu Zhong-kang han Ammara Waheed Zhongbin Ye Gao Li Alfons Baiker College of Chemistry and Chemical Engineering Southwest Petroleum UniversityChengdu 610500China School of Physical Science and Technology Ningbo UniversityNingbo 315211China Center for Energy Science and Technology Skolkovo Institute of Science and TechnologySkolkovo Innovation CenterMoscow 143026Russia State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China ETH Zurich Department of Chemistry and Applied BiosciencesInstitute for Chemical and BioengineeringHonggerbergHClCH-8093 ZurichSwitzerland

We evaluated bismuth doped cerium oxide catalysts for the continuous synthesis of dimethyl carbonate(DMC)from methanol and carbon dioxide in the absence of a dehydrating ***_(x)Ce_(1-x)O_(δ)nanocomposites of various compositions(x=0.06-0.24)were coated on a ceramic honeycomb and their structural and catalytic properties were *** incorporation of Bi species into the CeO_(2) lattice facilitated controlling of the surface population of oxygen vacancies,which is shown to play a crucial role in the mechanism of this reaction and is an important parameter for the design of ceria-based *** DMC production rate of the Bi_(x)Ce_(1-x)O_(δ) catalysts was found to be strongly enhanced with increasing Ov *** concentration of oxygen vacancies exhibited a maximum for Bi_(0.12)Ce_(0.88)O_(δ),which afforded the highest DMC production ***-term tests showed stable activity and selectivity of this catalyst over 45 h on-stream at 140°C and a gas-hourly space velocity of 2,880 mL·g_(cat)^(-1)·h^(-1).In-situ modulation excitation diffuse reflection Fourier transform infrared spectroscopy and first-principle calculations indicate that the DMC synthesis occurs through reaction of a bidentate carbonate intermediate with the activated methoxy(-OCH_(3))*** activation of C0_(2) to form the bidentate carbonate intermediate on the oxygen vacancy sites is identified as highest energy barrier in the reaction pathway and thus is likely the rate-determining step.

关键词： dimethyl carbonate synthesis bismuth-cerium oxides monolithic catalyst oxygen vacancies reaction mechanism

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基于定量CT的机会性骨质疏松诊断:2种定量CT骨密度测量软件临床应用比较分析

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河北医科大学学报 2024年第1期45卷 17-23页

作者：赵君禄刘斋韩康赵德园任庆云河北医科大学第一医院放射与核医学科河北石家庄050031

目的探讨FTHK定量CT骨密度测量软件和Mindways(MW)定量CT Pro骨密度测量软件测量腰椎骨密度(bone mineral density,BMD)的重复性、可靠性、一致性及其在临床中的应用价值。方法选择临床需要进行常规腹部CT扫描患者708例,将扫描数据分别... 详细信息

目的探讨FTHK定量CT骨密度测量软件和Mindways(MW)定量CT Pro骨密度测量软件测量腰椎骨密度(bone mineral density,BMD)的重复性、可靠性、一致性及其在临床中的应用价值。方法选择临床需要进行常规腹部CT扫描患者708例,将扫描数据分别传到FTHK和MW骨密度测量软件进行测量,对2组测量结果采用配对样本t检验、Pearson相关性分析、组内相关系数(intraclass correlation coefficient,ICC)、Bland-Altman分析及Cronbach′sα系数统计学方法进行分析。结果FTHK和MW 2种软件测量BMD的ICC分别为0.976及0.936。FTHK软件BMD值(126.29±50.16)mg/cm~3高于MW软件BMD值(112.85±46.02)mg/cm~3,两者的平均差值为10.6%(P<0.05)。FTHK软件对OP的检出率(19.63%)低于MW软件测量(25.42%)(P<0.05)。FTHK软件BMD与MW软件测量BMD之间呈显著正相关(r=0.955,P<0.01),Bland-Altman分析显示BMD差值的95%位于差值平均值±1.96标准差范围内。FTHK软件Cronbach′sα系数=0.963,MW软件Cronbach′sα系数=0.988。结论FTHK骨密度测量分析软件与MW骨密度测量软件的一致性及可信度好,可用于常规腹部CT扫描患者的机会性骨质疏松诊断。

关键词：骨质疏松骨密度体层摄影术,X线计算机

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高职临床医学应用型人才培养目标及能力目标构建——以成果为导向

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教育教学论坛 2024年第28期 52-55页

作者：刘晓静张志军康瀚文张伟石家庄医学高等专科学校临床医学系河北灵寿050599 石家庄医学高等专科学校教师发展中心河北灵寿050599 石家庄医学高等专科学校教务处河北灵寿050599

以成果产出为导向,临床医学专任教师通过顶岗实践并访谈专家,研讨临床医学生胜任职业岗位应具备的能力,并结合国家标准和办学定位,明确高职临床医学应用型人才培养目标。通过对在岗医师实际工作岗位所需能力和毕业生职业岗位胜任力进行... 详细信息

以成果产出为导向,临床医学专任教师通过顶岗实践并访谈专家,研讨临床医学生胜任职业岗位应具备的能力,并结合国家标准和办学定位,明确高职临床医学应用型人才培养目标。通过对在岗医师实际工作岗位所需能力和毕业生职业岗位胜任力进行问卷调查,分析整合了高职临床医学毕业要求核心能力及指标点,构建了高职临床医学能力目标。同时,厘清每门课程与毕业要求核心能力及指标点的映射关系,汇集了支撑能力目标达成“4321”课程体系。

关键词：成果导向人才培养目标能力目标

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10 kV电容器组运行异常的原因分析与处理

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电工技术 2024年第10期 145-146,150页

作者：韩康占敏杨刚卢正中邱奕铭张攀国网湖北省电力有限公司孝感供电公司湖北孝感432000

不平衡电压保护是电容器组的主要保护,首先介绍了电容器组故障的基本情况,分析了电容器组不平衡电压保护动作的原因,剖析了2起电容器组开关合闸后故障跳闸的案例,通过试验检查和数据分析找到了故障产生的原因,验证了保护动作原因分析的... 详细信息

不平衡电压保护是电容器组的主要保护,首先介绍了电容器组故障的基本情况,分析了电容器组不平衡电压保护动作的原因,剖析了2起电容器组开关合闸后故障跳闸的案例,通过试验检查和数据分析找到了故障产生的原因,验证了保护动作原因分析的有效性,最后提出了相关防范措施和处理建议。

关键词：电容器组开关跳闸不平衡电压保护原因分析

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Co-CRISPR: A valuable toolkit for mutation enrichment in the gene editing of Spodoptera frugiperda

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Insect Science 2023年第3期30卷 625-636页

作者： Wei-kang han Feng-Xian Tang Hao-Li Gao Yan Wang Na Yu Jian-Jun Jiang Ze-Wen Liu Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education) College of Plant ProtectionNanjing Agricultural UniversityNanjingChina Plant Protection Research Institute Guangxi Academy of Agricultural ScienceGuangxi Key Laboratory of Biology for Crop Diseases and Insect PestsNanningChina

The CRISPR/Cas9 system has been successfully applied in dozens of diverse species;although the screening of successful CRISPR/Cas9 editing events remains particularly laborious,especially for those that occur at relatively low ***,a co-CRISPR strategy was proved to enrich the desired CRISPR ***,the co-CRISPR strategy was developed in the Fall armyworm,Spodoptera frugiperda,with kynurenine 3-monooxygenase gene(kmo)as a *** kmo mosaics induced by single-guide RNAs(sgRNAs)/Cas9 displayed the darker green color phenotype in larvae,compared with wild type(brown),and mosaic-eye adults were significantly acquired from the mosaic larvae *** the kmo knockout strain,no significant difference was observed in larval development and adult *** 2(ace2)and Wntl were selected as target genes to construct the co-CRISPR strategy using kmo *** coinjection of kmo and ace2 sgRNAs,the mutant efficiency of ace2 was significantly increased in the kmo mosaic(larvae or adults)***,more malformed pupae with Wntl mutations were observed in the darker green larvae *** together,these results demonstrated that kmo was a suitable visible marker gene for the application and extension of co-CRISPR strategy in Fall *** darker green color in larvae or mosaic-eye in adults from kmo knockout as a marker,the mutant efficiency of a target gene could be enriched in a Fall armyworm group consisting of marked *** co-CRISPR strategy is helpful for gene function studies by the knockout technique with no or lethal phenotypes.

关键词： co-CRISPR kmo mutation enrichment Spodoptera frugiperda Wnt1

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