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Expression and characterization of a novel GH128 laminarinase OUC-BsLam26 harboring both hydrolyzing and transferring activities

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Food Science and Human Wellness 2024年

作者： Yueyang Dong Yongyi Quan Hong Jiang hamed I.Hamouda Zewei Lu Yimiao Chen Chengqiang Li Xiangzhao Mao State Key Laboratory of Marine Food Processing and Safety Control， College of Food Science and Engineering， Ocean University of China Qingdao Key Laboratory of Food Biotechnology Sanya Ocean Research Institute， Ocean University of China Processes Design and Development Department， Egyptian Petroleum Research Institute Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Biological Processing of Aquatic Products，China National Light Industry

Laminarin oligosaccharides (LOSs) with a specific degree of polymerization prepared through the laminarin degradation via laminarinase present more significant nutritional functions and application values. Human intestinal bacteria are promising potential producers of novel carbohydrate-active enzymes with unique properties. Here， a novel glycoside hydrolase family 128 (GH128) laminarinase OUC-BsLam26 from the intestinal bacterium Bacteroides sp. CBA7301 was heterologously expressed and characterized. The recombinant OUC-BsLam26 with a molecular mass of 49.86 kDa exhibits highest activity (6.60 U/mg) at 45 ℃ and pH 6.0， which shows noticeable temperature and pH stability. The purified OUC-BsLam26 could degrade laminarin via an endo-type mode with the generation of laminaripentaose， laminaritetraose， laminaritriose， and laminaribiose， among them， laminaritetraose is the principal product， which accounts for 45.25% of the total products， which is significantly different from the reported GH128 laminarinases. The minimum recognition substrate of OUC-BsLam26 is laminarihexaose. Furthermore， OUC-BsLam26 also could catalyze the transglycosylation process with the production of some novel glycosides. Altogether， the intestinal bacterium Bacteroides sp. CBA7301 contains laminarinase with unique product composition and OUC-BsLam26 is a hopeful bio-catalyst with the potential to produce laminaritetraose and some novel glycosides.

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Influence of processing history on microstructure， mechanical properties， and electrical conductivity of Cu-0.7Mg alloy

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Transactions of Nonferrous Metals Society of China 2024年

作者： Alireza KALHOR Kinga RODAK Marek TKOCZ Bartosz CHMIELA Ivo SCHINDLER ?ukasz POLOCZEK Krzysztof RADWA?SKI hamed MIRZADEH Marian KAMPIK Faculty of Materials Engineering， Silesian University of Technology V?B – Technical University of Ostrava， Faculty of Materials Science and Technology Materials Research Group， ?ukasiewicz Upper Silesian Institute of Technology School of Metallurgy and Materials Engineering， College of Engineering， University of Tehran Faculty of Electrical Engineering， Silesian University of Technology

The effects of forward extrusion as well as extrusion combined with reversible torsion (KoBo extrusion)， followed by additional deformation via the MaxStrain module of the Gleeble thermomechanical simulator， on the microstructure， mechanical properties， and electrical conductivity of a Cu-0.7Mg (wt.%) alloy， were investigated in this work. The simulation results highlighted the critical influence of processing history in determining the equivalent strain distribution. The sample subjected to forward extrusion at 400 ℃and subsequent MaxStrain processing (FM sample)， possessed 76% lower grain size compared to the sample processed solely with MaxStrain (AM sample). Likewise， the KoBo-extruded and MaxStrain-processed sample (KM sample) exhibited 66% smaller grain size compared to the AM sample. Tensile test results revealed that the AM， FM， and KM samples， respectively， possessed 251%， 288%， and 360% higher yield strength， and 95%， 121%， and 169% higher tensile strength compared to the initial annealed alloy， as a result of grain refinement as well as deformation strengthening. Finally， the electrical conductivity measurements revealed that AM， FM， and KM samples， respectively， possessed electrical conductivity values of 37.9， 35.6， and 32.0 (MS/m)， which， considering their mechanical properties， makes them eligible to be categorized as high-strength and high-conductivity copper alloys.

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Effect of Thermal Cycles on Creep Behavior of Bituminous Binder in Hot Regions

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Journal of Civil Engineering and Architecture 2024年第7期18卷 334-339页

作者： Bachir Glaoui Mahdjoub Bendjima M'hamed Merbouh Martin Van de Ven Laboratoire de la Fiabilité des Matériaux et Structures en Régions Sahariennes(FIMAS) Université de Béchar B.P.417Béchar08000Algérie Laboratory of Road and Railway Engineering TU DelftP.O.Box 50482600 GA DelftThe Netherlands

This research is devoted to the study of creep behavior of asphalt binder in hot *** binder was subjected to thermal cycles due to the variation of temperature from day to *** cycles produce a heating-cooling *** evaluate the effect of climate change in laboratory,the DSR(dynamic shear rheometer)was used,and the results found with the nine samples were *** thermal cycles led to aging and therefore hardening of the ***,a rheological model was proposed that can represent the curves obtained experimentally,where it is able to describe the creep behavior of binders tested.A new model is proposed that correlates well with the experimental curves,which is called A+2 K.

关键词： Bitumen temperature heating/cooling DSR BBR model

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A new hydrophobic deep eutectic solvent composed of menthol and tris(2-ethylhexyl) phosphate for solvent bar microextraction of parabens from biological fluids

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Emerging Contaminants 2024年第2期10卷 135-145页

作者： Nabil N.AL-Hashimi Husam Abed Alfattah Khaleel I.Assaf Asma A.Fakhoury Saja H.hamed Amjad H.El-Sheikh Khairi M.Fahelelbom The Hashemite University Faculty of Pharmaceutical SciencesDepartment of Pharmaceutical ChemistryP.O.Box 330127Zarqa13133Jordan The Hashemite University Faculty of ScienceDepartment of ChemistryP.O.Box 150459Zarqa13115Jordan Al-Balqa Applied University Faculty of ScienceDepartment of Chemistry19117 Al-SaltJordan The Hashemite University Faculty of Pharmaceutical SciencesDepartment of Pharmaceutics and Pharmaceutical TechnologyP.O.Box 330127Zarqa13133Jordan Al Ain University College of PharmacyDepartment of Pharmaceutical SciencesP.O.Box 64141Al AinAl AinUnited Arab Emirates

Parabens are antimicrobial preservatives with extensive applications in cosmetics,toiletries,pharmaceuticals,and *** the legitimate concerns relating to their potential to disrupt multiple endocrine functions,it becomes imperative to prioritize the development of innovative bioanalytical techniques for effectively monitoring their presence in biological *** this study,an efficient solvent bar microextraction(SBME)was established,utilizing new hydrophobic deep eutectic solvents(DEs)to determine methylparaben and propylparaben in urine and plasma *** DEs comprising menthol and tris(2-ethylhexyl)phosphate(M-TEHP)at various molar ratios were synthesized for the first time to enhance the extraction capacity and promote the eco-friendliness of the DE used as an extraction ***-transform infrared(FT-IR)and proton nuclear magnetic resonance(1H NMR)spectroscopies were employed to confirm and investigate the properties of the successful synthesis(M-TEHP)DE at a molar ratio of 1:*** synthesized DE exhibits low microbial toxicity and can be considered an eco-friendly solvent for ***,quantum-chemical calculations were utilized to predict synthesized DE's structure and interaction energy with selected *** influential operational factors of DE-SBME on the extraction efficiency(EE%)of both parabens were evaluated using response surface methodology based on central composite design,and a total of 30 extraction tests were conducted to determine the optimal *** optimized DE-SBME,in combination with HPLC-DAD,exhibited low detection limits(0.54–0.91μg L^(−1)),excellent linearity(R^(2)≥0.9993),precise results(RSDs≤7.6%),satisfactory recoveries(92–97%)and negligible matrix ***,it had remarkable effectiveness and applicability in determining selected parabens in real urine and plasma samples.

关键词： Hydrophobic deep eutectic solvent Solvent bar microextraction Parabens Response surface methodology and central composite design HPLC-DAD Urine and plasma

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白内障手术候选者的眼部生物特征参数分析

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国际眼科杂志 2024年第8期24卷 1186-1193页

作者： Zahra Sarlak Masoud Khorrami-Nejad hamed Ghasemi Ali Nouraeinejad Reza Yousefi 伊朗德黑兰医科大学Farabi眼科医院转化眼科研究中心 *** 伊朗德黑兰医科大学康复学院视光系 ***

目的:分析白内障手术候选人的眼部生物特征参数。方法:横断面研究。纳入2017-01/2021-09就诊于伊朗德黑兰Farabi眼科医院的4607例白内障患者(4607眼)。使用IOLMaster 700评估眼轴(AL)、角膜曲率(Kf、Ks、Km)、前房深度(ACD)。根据AL分为... 详细信息

目的:分析白内障手术候选人的眼部生物特征参数。方法:横断面研究。纳入2017-01/2021-09就诊于伊朗德黑兰Farabi眼科医院的4607例白内障患者(4607眼)。使用IOLMaster 700评估眼轴(AL)、角膜曲率(Kf、Ks、Km)、前房深度(ACD)。根据AL分为22 mm25 mm组,根据ACD分为3.00 mm3.60 mm组。结果:患者平均年龄60.3±14.1(8-95)岁,女2243例(48.69%),男2364例(51.31%)。AL、ACD和平均角膜曲率范围分别为23.1-23.4 mm、3.1-3.2 mm和44.50-45.00 D。AL>25 mm组ACD平均值显著高于22 mm≤AL≤25 mm组和22 mm3.60 mm组的平均AL显著高于3.00 mm25 mm组的Kf、Ks、Km均值显著高于ACD>3.60 mm组和22 mm

关键词：白内障眼轴前房深度角膜曲率

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Development and Characterization of Particle Boards Based on Sorghum Husk

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Open Journal of Applied Sciences 2024年第9期14卷 2460-2470页

作者： hamed Tidjani Tabe Gbian Edem Chabi Valéry Kouandété Doko Kora Farid Carlos Yarou Emmanuel Olodo Laboratory of Applied Energy and Mechanics (LEMA) Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin Laboratory of Rural Engineering School of Rural Engineering National University of Agriculture Ketou Benin

This study tackles current environmental challenges by developing innovative and eco-friendly particle boards utilizing sorghum husk, combined with recycled expanded polystyrene (EPS). This dual eco-responsible approach valorizes sorghum husk, often deemed agricultural waste, and repurposes EPS, a plastic waste, thus contributing to CO2 emission reduction and effective waste management. The manufacturing process involves dissolving recycled polystyrene within a solvent to create a binder, which is then mixed with sorghum husk and cold-pressed into composite boards. The study explores the impact of two particle sizes (fine and coarse) and two different concentrations of the recycled EPS binder. Results demonstrate significant variations in the boards’ mechanical properties, displaying a range of Modulus of Rupture (MOR) from 0.84 MPa to 3.85 MPa, and Modulus of Elasticity (MOE) spanning from 658.13 MPa to 1313.25 MPa, influenced by the binder concentration and particle size. These characteristics suggest that the boards can be effectively used in various construction applications, including interior decoration, false ceilings, and potentially for furniture and door manufacturing when combined with appropriate coatings. This study not only exemplifies the valorization of plastic and agricultural wastes but also offers a practical, localized solution to global climate change challenges by promoting sustainable construction materials.

关键词： Eco-Material Bio-Sourced Material Sorghum Husk Particle Board Recycled Expanded Polystyrene Sustainable Construction

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Traumatic Neglected Antero-Medial Knee Dislocation: Case Report

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Open Journal of Orthopedics 2024年第9期14卷 421-426页

作者： Souleymane Ouédraogo Massadiami Soulama Abdourahmane Ouangré Ousmane Ouermi Sékou Sidibé Maik Telyann Tchitembo Malick Diallo Patrick Wendpouiré hamed Dakouré Orthopedics and Traumatology Department University Hospital Sour Sanou Bobo-Dioulasso Burkina Faso Superior Institute of Medical Sciences University Nazi Boni Bobo-Dioulasso Burkina Faso Surgery Department Regional University Hospital Ouahigouya Burkina Faso Faculty of Medicine University Ledea Bernard Oudraogo Ouahigouya Burkina Faso

Introduction: Neglected knee dislocation is an unusual injury, only a few cases have been reported in the literature. The circumstances of onset are generally an unnoticed knee dislocation in a polytrauma context or a delay in consultation after the initial trauma. Presentation of case: We report the case of a 48-year-old female patient who sustained a knee injury during a domestic accident. Initial treatment was traditional and was followed for 3 years. She then consulted hospital, where a neglected knee dislocation was identified and treated with femorotibial arthrodesis. Discussion and conclusion: The treatment options of neglected knee dislocation include open reduction and fixation, arthrodesis and total knee arthroplasty. That the choice depends on the old unreduced of dislocation.

关键词： Neglected Knee Dislocation Tibiofemoral Arthrodesis Total Knee Arthroplasty

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Effect of single roll drive rolling on microstructure， texture， and tensile anisotropy of Al-5.6Zn-2.5Mg-1.4Cu aluminum alloy

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Transactions of Nonferrous Metals Society of China 2022年

作者： Amir kazemi-Navaee Roohollah Jamaati hamed Jamshidi Aval Department of Materials Engineering， Babol Noshirvani University of Technology

The current research aims to study the microstructure and texture development during single roll drive rolling (SRDR) of the Al-5.6Zn-2.5Mg-1.4Cu alloy. The relationship between anisotropy with microstructure and texture was investigated in detail. The SRDR process was immediately performed on the solution-treated sample with four different thickness reductions of 10， 20， 40， and 60%. Microstructural examination by optical and scanning electron microscopes， texture by X-ray diffraction， and tensile properties were conducted. The results showed that the value of in-plane anisotropy was significantly reduced from 11.6% in solution-treated alloy to 2.4% and 3.1% in 40% and 60% rolled samples， respectively. This was due to the formation of the Rotated Cube component， decreasing the overall texture intensity， and the formation of new grains through dynamic recrystallization. The yield strength in the rolling direction (RD) was higher than the value for the transverse direction (TD) which was due to grain morphology and texture in these samples. The severity of Portevin–Le Chatelier (PLC) along the RD (0°) was much lower than along the HD (45°) and TD (90°) directions owing to the presence of several strong orientations along the RD. By increasing the thickness reduction， the difference between strain hardening rate of RD， HD， and TD decreased due to the decrement of texture intensity. There was no difference between the fracture surfaces of 60% samples in the RD， HD， and TD， revealing nearly isotropic fracture behavior.

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Planning Target Volume Margin in Linac-Based Stereotactic Radiosurgery for Brain Metastases

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Journal of Cancer Therapy 2024年第7期15卷 265-272页

作者： Hany S. Attallah Radwa M. hamed Khaled M. EL-Shahat Aliaa Mahmoud Mahmoud Abdallah Ibraheem Haggag Emad Moustafa Adel Yassin Department of Radiation Oncology International Medical Center (IMC) Cairo Egypt Department of Radiation Oncology Armed Forces Medical Collogue (AFCM) Cairo Egypt Department of Medical Physics Al Azhar University Cairo Egypt Department of Medical Physics International Medical Center (IMC) Cairo Egypt Department of Medical Physics Department of Clinical Oncology Faculty of Medicine Zagazig University Zagazig Egypt

Background: The treatment of brain metastases with radiotherapy has shifted to the use of Stereotactic Radio-surgery (SRS). The technical issue of expanding the treatment volume around the Gross Tumor Volume (GTV) is a current debate. Radiotherapy centers use variable GTV-PTV margins, ranging from one to 2 mm. Material and Methods: We performed a dosimetric comparison in plans of twenty patients using three margins: PTV zero, PTV1, and PTV2. We also developed imaginary Peel volumes. These volumes are described as follows: Peel1 = PTV1 − GTV, Peel2 = PTV2 − GTV. Results: Our results showed that the mean PTV volume differed significantly across the different margins (p = 0.000). The V12 of the brain significantly varied as a function of PTV margin (p = 0.000). The target coverage and plan quality indices were not significantly different. The Peel volume dosimetric analysis showed that the mean dose was significantly higher in the nearby normal brain tissue: Peel1 (p = 0.022) and Peel 2 (p = 0.013). Conclusion: According to our dosimetric analysis, expanding the GTV into a PTV by 1 mm margin is more convenient than 2 mm.

关键词： SRS Brain Metastases PTV

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单辊驱动轧制对Al-5.6Zn-2.5Mg-1.4Cu铝合金显微组织、织构和力学性能各向异性的影响

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Transactions of Nonferrous Metals Society of China 2023年第11期33卷 3266-3281页

作者： Amir kazemi-NAVAEE Roohollah JAMAATI hamed JAMSHIDI AVAL Department of Materials Engineering Babol Noshirvani University of TechnologyShariati Ave.Babol 47148–71167Iran

研究在单辊驱动轧制Al-5.6Zn-2.5Mg-1.4Cu合金过程中,材料显微组织和织构的变化,并详细研究其力学性能各向异性与显微组织和织构的关系。对样品固溶处理后立即进行单辊驱动轧制,厚度减薄率分别为10%、20%、40%和60%。通过光学和扫描电... 详细信息

研究在单辊驱动轧制Al-5.6Zn-2.5Mg-1.4Cu合金过程中,材料显微组织和织构的变化,并详细研究其力学性能各向异性与显微组织和织构的关系。对样品固溶处理后立即进行单辊驱动轧制,厚度减薄率分别为10%、20%、40%和60%。通过光学和扫描电子显微镜对显微组织进行表征,通过X射线衍射仪对织构进行表征,并对拉伸力学性能进行分析。结果表明,减薄率为40%和60%的轧制样品,其面内各向异性值从固溶处理合金的11.6%分别显著降低到2.4%和3.1%。这是由于旋转立方织构的形成减小了总的织构强度以及动态再结晶过程中新晶粒的形成。由于样品中晶粒形貌和织构不同,样品轧制方向(RD)比横向(TD)的屈服强度高。沿RD存在的几个较强取向导致沿RD(0°)的Portevin–LeChatelier(PLC)效应明显低于沿HD(45°)和TD(90°)的。随着厚度减薄率的增大,织构强度减小,导致RD、HD和TD之间的应变硬化率差异减小。而对于减薄率为60%的样品,其RD、HD和TD的断裂面无差异,说明其断裂行为为各向同性。

关键词： AA7075铝合金单辊驱动轧制织构力学性能各向异性

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