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Interaction of haptoglobin with hemoglobin octamers based on the mutation <i>α</i>Asn78Cys or <i>β</i>Gly83Cys

American Journal of Molecular Biology 2012年第1期2卷 1-10页

作者： Thomas Brillet Michael C. Marden Joanne I. Yeh Tong-Jian Shen Nancy T. Ho Regina Kettering Shoucheng Du Corinne Vasseur Elisa Domingues-hamdi Chien Ho Véronique Baudin-Creuza Department of Biological Sciences Carnegie Mellon University Pittsburgh USA Department of Structural Biology School of Medicine University of Pittsburgh Pittsburgh USA Inserm U779 Université Paris XI et VII CHU Bicêtre Le Kremlin-Bicêtre France

Octameric hemoglobins have been developed by the introduction of surface cysteines in either the alpha or beta chain. Originally designed as a blood substitute, we report here the structure and ligand binding function;in addition the interaction with haptoglobin was studied. The recombinant Hbs (rHbs) with mutations alpha Asn78Cys or beta Gly83Cys spontaneously form octamers under conditions where the cysteines are oxidized. Oxygen binding curves and CO kinetic studies indicate a correct allosteric transition of the tetramers within the octamer. Crystallographic studies of the two rHbs show two disulfide bonds per octamer. Reducing agents may provoke dissociation to tetramers, but the octamers are stable when mixed with fresh human plasma, indicating that the reduction by plasma is slower than the oxidation by the dissolved oxygen, consistent with an enhanced stability. The octameric rHbs were also mixed with a solution of haptoglobin (Hp), which binds the dimers of Hb: there was little interaction for incubation times of 15 min;however, on longer timescales a complex was formed. Dynamic light scattering was used to follow the interaction of Hp with the alpha Asn78Cys octamer during 24 hours;a transition from a simple complex of 15 nm to a final size of 60 nm was observed. The results indicate a specific orientation of the αβ dimers may be of importance for the binding to haptoglobin.

关键词： Octamers Hemoglobin Haptoglobin allosteric Transition Crystallography

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Optimizing alzheimer’s Disease Therapy Using a Neural Intelligent Agent-Based Platform

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International Journal of Intelligence Science（智能科学国际期刊） 2021年第2期

作者： hamdi Ben Abdessalem alexie Byrns Claude Frasson

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Cognitive Workload Assessment of Aircraft Pilots

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Journal of Behavioral and Brain Science（行为与脑科学期刊） 2022年第10期

作者： maxime antoine hamdi ben abdessalem claude frasson

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Statistical Examination of Frost Characterization: A Case of Global Warming Impact in Jordan

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Journal of Water Resource and Protection（水资源与保护） 2011年第8期 620-627页

作者： Moshrik R hamdi Mahmoud Abu alaban Mohammed Jaber

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Detection and Recuperation of Mental Fatigue

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Journal of Behavioral and Brain Science（行为与脑科学期刊） 2023年第2期

作者： alyssa Hajj Assaf hamdi Ben Abdessalem Claude Frasson

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Hepatobiliary manifestations following two-stages elective laparoscopic restorative proctocolectomy for patients with ulcerative colitis:A prospective observational study

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World Journal of Gastrointestinal Surgery 2023年第2期15卷 234-248页

作者： Tamer A A M Habeeb Abdulzahra Hussain Mauro Podda Pasquale Cianci Bruce Ramshaw Khaled Safwat Wesam M Amr Tamer Wasefy alaa A Fiad Mohamed Ibrahim Mansour Adel Mahmoud Moursi GamalOsman Anass Qasem Mohamed Fawzy Mohamed Ibrahim Abo alsaad Abd-Elfattah Kalmoush MohammedShaaban Nassar Fawzy M Mustafa Mahmoud Hassib Morsi Badawy Ahmed Hamdy hamdi Elbelkasi Bassam Mousa Abd-Elrahman M Metwalli Walid A Mawla Mostafa M Elaidy Muhammad ali Baghdadi Ahmed Raafat Department of General Surgery Faculty of MedicineZagazig UniversityZagazig 44759SharkiaEgypt Homerton University Hospital London E96SRUK Department of Surgical Science University of CagliariCagliari 2432Italy Department of Medical and Surgical Sciences Universitàdegli studi di FoggiaFoggia 546Italy MD CQInsights PBC Co-founder&CEOTennesseeTN 37010United States Department of Internal Medicine Zagazig UniversityZagazig 2355Egypt Department of Internal Medicine Suez UniversitySuez 235Egypt Department of General Surgery Faculty of Medicine-Merit UniversitySohag 82511Egypt Department of General Surgery Al-azhar UniversityCairo 285Egypt Department of Hepato-Bilio-Pancreatic(HBP)Surgery National Hepatology and Tropical Medicine Research InstituteCairo 285Egypt Department of General Surgery Mataryia Teaching HospitalCairo 285Egypt

BACKGROUND Hepatobiliary manifestations occur in ulcerative colitis(UC)*** effect of laparoscopic restorative proctocolectomy(LRP)with ileal pouch anal anastomosis(IPAA)on hepatobiliary manifestations is *** To evaluate hepatobiliary changes after two-stages elective laparoscopic restorative proctocolectomy for patients with *** Between June 2013 and June 2018,167 patients with hepatobiliary symptoms underwent two-stage elective LRP for UC in a prospective observational *** with UC and having at least one hepatobiliary manifestation who underwent LRP with IPAA were included in the *** patients were followed up for four years to assess the outcomes of hepatobiliary *** The patients'mean age was 36±8 years,and males predominated(67.1%).The most common hepatobiliary diagnostic method was liver biopsy(85.6%),followed by Magnetic resonance cholangiopancreatography(63.5%),Antineutrophil cytoplasmic antibodies(62.5%),abdominal ultrasonography(35.9%),and Endoscopic retrograde cholangiopancreatography(6%).The most common hepatobiliary symptom was Primary sclerosing cholangitis(PSC)(62.3%),followed by fatty liver(16.8%)and gallbladder stone(10.2%).66.4%of patients showed a stable course after *** or regressive courses occurred in 16.8%of *** was 6%,and recurrence or progression of symptoms required surgery for 15%.Most PSC patients(87.5%)had a stable course,and only 12.5%became ***-thirds(64.3%)of fatty liver patients showed a regressive course,while one-third(35.7%)showed a stable *** rates were 98.8%,97%,95.8%,and 94%at 12 mo,24 mo,36 mo,and at the end of the *** In patients with UC who had LRP,there is a positive impact on hepatobiliary *** caused an improvement in PSC and fatty liver *** most prevalent unchanged course was PSC,while the most common improvement was fatty liver disease.

关键词： Courses Hepatobiliary manifestations Primary sclerosing cholangitis Restorative proctocolectomy

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