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Therapeutic potential of stem cells in subarachnoid hemorrhage

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Neural Regeneration Research 2025年第4期20卷 936-945页

作者： hideki Kanamaru Hidenori Suzuki Department of Neurosurgery Mie University Graduate School of MedicineTsuJapan

Aneurysm rupture can result in subarachnoid hemorrhage,a condition with potentially severe consequences,such as disability and *** the acute stage,early brain injury manifests as intracranial pressure elevation,global cerebral ischemia,acute hydrocephalus,and direct blood–brain contact due to aneurysm *** may subsequently cause delayed cerebral infarction,often with cerebral vasospasm,significantly affecting patient *** complications such as brain volume loss and chronic hydrocephalus can further impact *** the mechanisms of subarachnoid hemorrhage-induced brain injury is paramount for identifying effective *** cell therapy,with its multipotent differentiation capacity and anti-inflammatory effects,has emerged as a promising approach for treating previously deemed incurable *** review focuses on the potential application of stem cells in subarachnoid hemorrhage pathology and explores their role in neurogenesis and as a therapeutic intervention in preclinical and clinical subarachnoid hemorrhage studies.

关键词： delayed cerebral ischemia early brain injury matricellular protein neurogenesis stem cell therapy subarachnoid hemorrhage

大鼠ACAT的末端片段在大肠杆菌中的融合表达及纯化

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云南大学学报（自然科学版） 1999年第S3期21卷 279-279页

作者：张春妮 hakamata hideki Akira Miyazaki Hirofumi Matsuda Seikon Horiuchi 南京军区南京总医院全军医学检验中心南京210002 日本熊本大学医学院生化系

Changes over time in treatment for obstructive jaundice

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World Journal of Gastrointestinal Surgery 2024年第10期16卷 3074-3077页

作者： hideki Aoki Department of Surgery National Hospital Organization Iwakuni Clinical CenterIwakuni 740-8510YamaguchiJapan

This editorial discusses an article by Peng et *** study reviewed the efficacy and safety of a new approach for treating obstructive *** the pathophysiology of obstructive jaundice has not yet been fully elucidated,pro-gress has been made in its *** are two aspects of obstructive jaundice:Cholestatic status and absence of bile in the intestinal *** biliary drainage resolved both the ***,endoscopic retrograde biliary drainage(ERBD)has replaced percutaneous transhepatic biliary drainage,and ERBD is transitioning to endoscopic ultrasound guided biliary *** editorial briefly explains the mechanism and treatment of obstructive jaundice and the prospects of this new internal biliary drainage technique.

关键词： Obstructive jaundice Intestinal permeability Biliary drainage Endoscopic retrograde biliary drainage Endoscopic ultrasound guided biliary drainage

CFD simulation of pumping ventilation in a three-story isolated building with internal partitioning:Effects of partition widths,heights and locations

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Building Simulation 2024年第2期17卷 267-284页

作者： Huai-Yu Zhong Jie Sun Chao Lin Song-Heng Wu Jin Shang hideki Kikumoto Fu-Ping Qian Carlos Jimenez-Bescos Fu-Yun Zhao School of Energy and Environment Anhui University of TechnologyMa-An-ShanAnhui Province243002China State Key Laboratory of Green Building in Western China Xi’an University of Architecture&TechnologyXi’anShaanxi ProvinceChina School of Power and Mechanical Engineering Wuhan UniversityWuhanHubei Province430072China Institute of Industrial Science The University of Tokyo4-6-1 KomabaMeguro-kuTokyo153-8505Japan School of Built and Natural Environment University of DerbyDerbyUK

Pumping ventilation(PV),a special single-sided ventilation(SSV),has been certified as an effective strategy to improve the air exchange rate of ***,most studies targeted on the single space,and few studies have been focused on the effect of internal partitioning on *** paper aims to evaluate the ventilation performance of PV influenced by different configurations of internal *** fluid dynamics(CFD)simulation was used to predict the flow fields and ventilation *** width(w/H),height(h/H)and location(d/H)are the three main internal partition parameters considered in this *** simulation results showed that the total,mean and fluctuating ventilation rates all decrease with wider internal *** normalized total ventilation rate decreases by 7.6%when w/H is increased from 50%to 75%.However,the reduction rate is only 0.23%between w/H=0 and 25%,and only 0.61%between w/H=25%and 50%.The ventilation rate is hardly reduced by increasing the partition width when w/H50%.Increasing the partition height will reduce the mean ventilation rate but promote the fluctuating and total ventilation rate in some *** increase of total ventilation rate by 1.4%is observed from h/H=50%to 75%.The ventilation rate is larger when the internal partition is attached to the leeward or windward *** total,mean and fluctuating ventilation rates for d/H=50%are relatively higher than d/H=0 by 1.5%,3.1%and 0.8%,*** the internal partition should be mounted attached to the windward wall so as to obtain the greatest pumping ventilation *** periodicity of pumping flow oscillation and pumping frequency are independent of the partition *** peak power of pumping flow is the lowest for the widest internal partition and is negatively affected by the partition height,but it generally has a positive correlation with the distance between the partition and leeward wa

关键词： pumping ventilation single-sided ventilation internal partitioning periodic oscillation ventilation rate

载脂蛋白E-基因敲除鼠VLDL和IDL在动脉硬化中的作用

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南京大学学报（自然科学版） 1999年第2期35卷 180-185页

作者：张春妮 hakamata hideki Sakaguchi Hisashi Suzuki Hirosh Miyazaki Akira 庄一义 Horiuchi Seikoh 南京大学医学院临床学院南京军区南京总医院解放军医学检验中心日本熊本大学医学部日本中外药物研究所

探讨载脂蛋白Ｅ（ａｐｏＥ）－基因敲除鼠极低密度脂蛋白（ＶＬＤＬ）和中间密度脂蛋白（ＩＤＬ）组分（ａｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ）致动脉硬化作用．采用超离法从ａｐｏＥ－基因敲除鼠血浆中分离ＶＬＤＬ和ＩＤＬ组分，与鼠腹腔... 详细信息

探讨载脂蛋白Ｅ（ａｐｏＥ）－基因敲除鼠极低密度脂蛋白（ＶＬＤＬ）和中间密度脂蛋白（ＩＤＬ）组分（ａｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ）致动脉硬化作用．采用超离法从ａｐｏＥ－基因敲除鼠血浆中分离ＶＬＤＬ和ＩＤＬ组分，与鼠腹腔巨噬细胞共育，观察ａｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ与巨噬细胞的相互作用．ＡｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ导致细胞胆固醇酯含量显著增加．其诱导的细胞［３Ｈ］胆固醇油酸脂量高达１５．１ｎｍｏｌ／ｍｇ细胞蛋白，是天然低密度脂蛋白的８．４倍．形态学观察显示，经ａｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ处理的巨噬细胞与苏丹黑Ｂ呈阳性染色．细胞结合实验表明，１２５Ｉ－ａｐｏＥｋｏ－ＶＬＤＬ／ＩＤＬ与巨噬细胞的总结合可被非标记配基取代８０％以上，特异结合呈一饱和图形．同时细胞缔合和细胞内降解实验也显示相似结果．

关键词： VLDL IDL 载脂蛋白E 基因敲除动脉硬化

A Comparative Study of Two Tree-Based Models for Predicting Flyrock Velocity at Open Pit Bench Mining

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Open Journal of Applied Sciences 2024年第2期14卷 267-287页

作者： Ezatullah Rawnaq Bassir Esmatyar Akihiro Hamanaka Takashi Sasaoka hideki Shimada Department of Earth Resources Engineering Kyushu University Fukuoka Japan Department of Mining and Mineral Engineering Suleyman Demirel University Isparta Türkiye

Blasting is a cost-effective technique to break hard rock volumes by using explosives in the mining and civil engineering realms. Moreover, although blasting is a designed process and plays an indispensable role in these industries, it can also have multiple adverse environmental impacts. One such effect is flyrock, which poses risks to nearby machinery, and residential structures, and can even lead to injuries or fatalities. To optimize blasting efficiency as well as restrict side effects, prediction of the blast aftereffects is vital. Therefore, the present work focuses on using two machine learning methods to predict the velocity of flyrock in the open pit mine. To address this issue, a comprehensive dataset was gathered from the open pit mine. Then, Decision Tree and Random Forest algorithms were employed to predict flyrock velocity. The Random Forest model demonstrated superior performance compared to the Decision Tree model. Nonetheless, the performance of the Decision Tree model was deemed satisfactory, as evidenced by its coefficient of determination value of 0.83, mean squared error (MSE) of 4.2, and mean absolute percentage error (MAPE) of 5.6%. Considering these metrics, it is reasonable to conclude that tree-based algorithms can be effective in predicting flyrock velocity.

关键词： Flyrock Machine Learning Bench Blasting Coefficient of Determination

大鼠ACAT的N末端片段(氨基酸1～126)在大肠杆菌中的融合表达及纯化

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医学研究生学报 1999年第4期12卷 251-253页

作者：张春妮垮田秀树宫崎章松田浩史堀内正公南京军区南京总医院解放军医学检验中心南京210002 日本熊本大学医学部生化系

目的：重组法制备纯化乙酰辅酶Ａ：胆固醇酰基转移酶（ＡＣＡＴ）Ｎ末端膜外结构域（氨基酸１～１２６）。　方法：经ＰＣＲ扩增得到的ＡＣＡＴ的Ｎ末端膜外结构域（氨基酸１～１２６）用ＥｃｏＲＩ酶解，插入到表达载体ｐＧＥＸ－２... 详细信息

目的：重组法制备纯化乙酰辅酶Ａ：胆固醇酰基转移酶（ＡＣＡＴ）Ｎ末端膜外结构域（氨基酸１～１２６）。　方法：经ＰＣＲ扩增得到的ＡＣＡＴ的Ｎ末端膜外结构域（氨基酸１～１２６）用ＥｃｏＲＩ酶解，插入到表达载体ｐＧＥＸ－２ＴＫ中，得到重组表达质粒ｐＧＥＸ－２ＴＫ／ＡＣＡＴ（氨基酸１～１２６）。阳性重组子在大肠杆菌中经ＩＰＴＧ诱导表达ＧＳＴ－Ａ－ＣＡＴ（氨基酸１～１２６），重组表达菌裂解上清液经ＧＳＨ－ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ亲和柱纯化。　结果：ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ分析显示得到了纯的ＧＳＴ－ＡＣＡＴ（氨基酸１～１２６）融合蛋白。　结论：ＡＣＡＴ的Ｎ末端膜外结构域（氨基酸１～１２６）的分离纯化为抗ＧＳＴ－ＡＣＡＴ（Ｎ末端片段）抗体的制备及其ＡＣＡＴ结构和功能关系的进一步研究打下了基础。

关键词：乙酰辅酶A 胆固醇酰基转移酶(ACAT) 融合表达大肠杆菌动脉粥样硬化

Genomic medicine in clinical practice:national genomic medicine program in Japan

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Cancer Biology & Medicine 2024年第1期21卷 4-9页

作者： Teruhiko Yoshida Yasushi Yatabe Ken Kato Genichiro Ishii Akinobu Hamada Hiroyuki Mano Kuniko Sunami Noboru Yamamoto Takashi Kohno Department of Genetic Medicine and Services National Cancer Center HospitalTokyo 104-0045Japan Department of Diagnostic Pathology National Cancer Center HospitalTokyo 104-0045Japan Clinical Research Support Offce Clinical Research Coordinating SectionBiobank Translational Research Support SectionNational Cancer Center HospitalTokyo 104-0045Japan Department of Pathology and Clinical Laboratories National Cancer Center Hospital EastChiba 277-8577Japan Division of Molecular Pharmacology National Cancer Center Research InstituteTokyo 104-0045Japan National Cancer Center Research Institute Tokyo 104-0045Japan Department of Laboratory Medicine National Cancer Center HospitalTokyo 104-0045Japan Department of Experimental Therapeutics National Cancer Center HospitalTokyo 104-0045Japan Division of Genome Biology National Cancer Center Research InstituteTokyo 104-0045Japan

Cancer statistics in Japan Cancer is the most common cause of death in Japan based on Statistics 2021~*** statistics were first gathered,infectious diseases,such as tuberculosis,and cerebrovascular disease have been the main causes of death in *** surpassed cerebrovascular disease as the main cause of death in 1981,and the number of cancer deaths has *** 38,000 people died of cancer in *** National Cancer Center(NCC)reported that the 5-year survival rate for patients with cancer was improving(62%for males and 66.9%for females)in a population-based cancer registry.

关键词： Cancer cancer death

组蛋白激活晚期糖基化终末产物受体并引发炎症反应

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食品与发酵工业 2024年第2期50卷 78-84页

作者：孙常津杨岩刘国玉中西秀树江南大学生物工程学院江苏无锡214122 中国食品发酵工业研究院有限公司功能主食创制与慢病营养干预北京市重点实验室北京100015

晚期糖基化终末产物受体(receptor for advanced glycation end product,RAGE)是免疫球蛋白受体,能够与多种配体结合,研究发现RAGE参与了非专职吞噬细胞对组蛋白修饰的微米颗粒的吞噬过程,但目前没有研究证明组蛋白是RAGE的配体。该研... 详细信息

晚期糖基化终末产物受体(receptor for advanced glycation end product,RAGE)是免疫球蛋白受体,能够与多种配体结合,研究发现RAGE参与了非专职吞噬细胞对组蛋白修饰的微米颗粒的吞噬过程,但目前没有研究证明组蛋白是RAGE的配体。该研究对组蛋白分子能否直接激活RAGE及其下游信号通路进行探究。使用共聚焦显微镜和流式细胞仪检测哺乳动物细胞对可溶性组蛋白分子的内化;采用钙黄绿素乙酰氧基甲酯/碘化丙啶染色法检测细胞活性;通过荧光探针2′,7′-二氯二氢荧光素二乙酸酯检测胞内活性氧水平;利用酶联免疫吸附剂测定法(enzyme-linked immunosorbent assay,ELISA)测定炎症因子的释放。结果表明,组蛋白分子能够被野生型HEK293T细胞内化并于胞内溶酶体中聚集,RAGE-敲除细胞则无法内化组蛋白;培养基组蛋白质量浓度在100μg/mL时对细胞无明显毒性,细胞活性正常;组蛋白能够通过RAGE刺激细胞活性氧水平升高,并释放炎症因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6);组蛋白与DNA结合能够增强对RAGE的刺激。以上结果表明,组蛋白能够激活RAGE及其下游炎症通路。

关键词：晚期糖基化终末产物受体组蛋白胞饮活性氧炎症