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Defocused imaging-based quantification of plasmon-induced distortion of single emitter emission

Light(Science & Applications) 2023年第10期12卷 2236-2247页

作者： Gwiyeong Moon Taehwang Son hajun yoo Changhun Lee Hyunwoong Lee Seongmin Im Donghyun Kim School of Electrical and Electronic Engineering Yonsei UniversitySeoul 03722Korea LG Innotek Seoul 07796South Korea Center for Systems Biology Massachusetts General HospitalBostonMAUSA LG Display PajuGyeonggi-do 10845South Korea

Optical properties of single emitters can be significantly improved through the interaction with plasmonic structures,leading to enhanced sensing and imaging *** turn,single emitters can act as sensitive probes of the local electromagnetic field surrounding plasmonic structures,furnishing fundamental insights into their physics and guiding the design of novel plasmonic ***,the interaction of emitters in the proximity to a plasmonic nanostructure causes distortion,which hinders precise estimation of position and polarization state and is one of the reasons why detection and quantification of molecular processes yet remain fundamentally challenging in this era of ***,we investigate axially defocused images of a single fluorescent emitter near metallic nanostructure,which encode emitter positions and can be acquired in the far-field with high sensitivity,while analyzing the images with pattern matching algorithm to explore emitter-localized surface plasmon interaction and retrieve information regarding emitter *** distortion in defocused images of fluorescent beads and quantum dots near nanostructure was observed and analyzed by pattern matching and finite-difference time-domain methods,which revealed that the distortion arises from the emitter interaction with *** matching algorithm was also adopted to estimate the lateral positions of a dipole that models an emitter utilizing the distorted defocused images and achieved improvement by more than 3 times over conventional diffraction-limited localization *** improvement by defocused imaging is expected to provide a way of enhancing reliability when using plasmonic nanostructure and diversifying strategies for various imaging and sensing modalities.

关键词： distortion matching interaction

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