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腺相关病毒介导Parkin对帕金森模型大鼠的治疗作用

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中华实验外科杂志 2024年第2期41卷 251-254页

作者：李海洋赖生龙苗壮李玉梁庆华河南省人民医院(郑州大学人民医院河南大学人民医院)神经外科郑州450003

目的探讨腺相关病毒介导Parkin对帕金森模型大鼠的治疗作用。方法 60只SD大鼠随机分为对照组、模型组和Parkin组, 模型组和Parkin组大鼠采用颈背部皮下注射鱼滕酮乳化液建立帕金森(PD)模型。Parkin组大鼠在建模后于左侧黑质定向注射携带... 详细信息

目的探讨腺相关病毒介导Parkin对帕金森模型大鼠的治疗作用。方法 60只SD大鼠随机分为对照组、模型组和Parkin组, 模型组和Parkin组大鼠采用颈背部皮下注射鱼滕酮乳化液建立帕金森(PD)模型。Parkin组大鼠在建模后于左侧黑质定向注射携带Parkin基因的腺相关病毒, 对照组和模型组经左侧黑质定向注射携带空载体的阴性腺相关病毒。在注射21 d后, 对3组大鼠进行行为学分析;并处死大鼠, 采用原位缺口末端标记法(TUNEL)分析3组大鼠黑质细胞凋亡指数;采用超氧化物歧化酶(SOD)和谷胱甘肽(GSH)-px试剂盒检测SOD和GSH-px水平;采用蛋白质印迹法(Western blot)分析凋亡相关蛋白半胱氨酰天冬氨酸特异性蛋白酶-3(Caspase-3)、B细胞淋巴瘤/白血病-2(bcl-2)和bcl-2相关X蛋白(bax)的水平。不同处理比较采用方差分析, 组间比较采用LSD法。结果 Parkin组大鼠脑组织黑质区Parkin蛋白表达水平(1.54±0.18)明显高于对照组和模型组(0.81±0.11、0.89±0.12), 差异有统计学意义(t=9.257、8.014, P<0.05)。Parkin组大鼠10 min内旋转圈数[(4.87±0.85)圈]明显低于模型组[(12.25±3.21)圈], 差异有统计学意义(t=4.264, P<0.05)。Parkin组大鼠脑组织黑质区细胞凋亡指数[(10.67±4.91)%]明显低于模型组[(34.59±8.32)%], 差异有统计学意义(t=3.698, P<0.05)。Parkin组大鼠外周血清SOD和GSH-px酶活性[(3.87±0.61)、(7.98±1.27) U/ml]明显高于模型组[(2.18±0.51)、(4.10±1.09) U/ml], 差异有统计学意义(t=6.594、7.251, P<0.05)。Parkin组大鼠脑组织Caspase-3和bax水平(1.12±0.08、1.23±0.06)明显低于模型组大鼠脑组织Caspase-3和bax水平(1.58±0.08、1.69±0.11), 差异有统计学意义(t=8.174、9.241, P<0.05)。Parkin组大鼠大鼠脑组织bcl-2水平(1.38±0.10)高于模型组(1.02±0.08), 差异均有统计学意义(t=5.141, P<0.05)。结论采用腺相关病毒介导Parkin蛋白在帕金森大鼠黑质表达, 可以显著抑制细胞凋亡, 显著改善大鼠的行为。

关键词： Parkin 帕金森病凋亡腺相关病毒超氧化物歧化酶谷胱甘肽

医护合作清单式教学管理模式在乡村医生临床实践中的应用

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首都食品与医药 2024年第7期31卷 102-105页

作者：胡黎婷石雷赵鹏李海洋李金亮首都医科大学延庆教学医院北京102100

目的探究医护合作清单式教学管理模式在乡村医生临床实践中的应用效果。方法选择首都医科大学延庆教学医院2019级和2020级首都医科大学三年制临床医学专业(山区定向)医学生47名为研究对象,2019级29名学生为对照组(采用常规带教模式),202... 详细信息

目的探究医护合作清单式教学管理模式在乡村医生临床实践中的应用效果。方法选择首都医科大学延庆教学医院2019级和2020级首都医科大学三年制临床医学专业(山区定向)医学生47名为研究对象,2019级29名学生为对照组(采用常规带教模式),2020级18名学生为观察组(采用医护合作清单式教学管理模式),对比两组理论知识评分、专业技能评分、诊疗能力、教学模式满意度。结果观察组理论知识、专业技能评分高于对照组,差异有统计学意义(P0.05);带教后,两组问诊成绩、查体成绩、护理操作、病史收集、治疗方案、人文关怀、医患沟通、胜任能力评分均升高,对照组低于观察组,差异有统计学意义(P<0.05)。观察组课程形式、学习兴趣、培养学习能力、参与积极性评分高于对照组,差异有统计学意义(P<0.05)。结论临床医学专业(山区定向)医学生接受医护合作清单式教学管理模式,可提高乡村医生诊疗能力,提高带教效果、教学满意度。

关键词：医护合作清单式教学管理模式乡村医生临床实践教学效果

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丁基黄原酸盐合成过程起泡问题分析与处理

化工生产与技术 2024年第2期30卷 40-43,I0005页

作者：李海洋胡春红冯卫国金堆城钼业集团有限公司陕西渭南714100

以金堆城钼业集团有限公司黄原酸盐合成项目为例,针对丁基黄原酸盐合成过程中起泡的问题,采用室内模拟实验和车间生产验证相结合的方式,通过对生产过程、物料性质的分析,查找出了起泡的原因和形成过程,决定采用添加消泡剂的方式来消除... 详细信息

以金堆城钼业集团有限公司黄原酸盐合成项目为例,针对丁基黄原酸盐合成过程中起泡的问题,采用室内模拟实验和车间生产验证相结合的方式,通过对生产过程、物料性质的分析,查找出了起泡的原因和形成过程,决定采用添加消泡剂的方式来消除泡沫。结果表明:起泡的原因为回收的二硫化碳中带有的有机杂质;1800#、DS-350消泡剂添具有较好的消泡效果,且消泡剂添加量较小,性质较为稳定,对黄原酸盐产品质量和选矿效果无不良影响。

关键词：丁基黄原酸盐起泡消泡剂

Ginsenoside F1 administration promotes UCP1-dependent fat browning and ameliorates obesity-associated insulin resistance

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Food Science and Human Wellness 2023年第6期12卷 2061-2072页

作者： Yuhan Meng Weili li Chenxing Hu Si Chen haiyang li Feifei Bai Lujuan Zheng Ye Yuan Yuying Fan Yifa Zhou Engineering Research Center of Glycoconjugates of Ministry of Education Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural DrugsSchool of Life SciencesNortheast Normal UniversityChangchun 130024China

Obesity-induced type 2 diabetes is mainly due to excessive free fatty acids leading to insulin *** thermogenesis is regarded as an effective strategy for hypolipidemia and *** is a natural active component in Panax ginseng ***,and some of them enhance ***,there are few studies on the mechanism and target of ginsenosides enhancing *** thermogenic protein uncoupling protein 1(UCP1)-luciferase reporter assay,we identifi ed ginsenoside F1 as a novel UCP1 activator in the ginsenosides *** pull down assay and inhibitor interference,we found F1 binds toβ3-adrenergic receptors(β3-AR)to enhance UCP1 expression via cAMP/PKA/CREB *** also investigated the ability of F1 on energy metabolism in obesity-induced diabetic mice,including body weight,body composition and energy *** results of proteomics showed that F1 signifi cantly up-regulated thermogenesis proteins and lipolytic proteins,but down-regulated fatty acid synthesis *** F1 increased thermogenesis and ameliorated insulin resistance specifi cally by promoting the browning of white adipose tissue in obese ***,ginsenoside F1 improves norepinephrine-induced insulin resistance in adipocytes and hepatocytes,and shows a stronger mitochondria respiration ability than *** fi ndings suggest that ginsenoside F1 is a promising lead compound in the improvement of insulin resistance.

关键词： Ginsenoside F1 Uncoupling protein 1 β3-Adrenergic receptor White adipose tissue browning Insulin resistance

Stability analysis of a liquid crystal elastomer self-oscillator under a linear temperature field

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Applied Mathematics and Mechanics(English Edition) 2024年第2期45卷 337-354页

作者： haiyang WU Jiangfeng LOU Biao ZHANG Yuntong DAI Kai li School of Civil Engineering Anhui Jianzhu UniversityHefei 230601China

Self-oscillating systems abound in the natural world and offer substantial potential for applications in controllers,micro-motors,medical equipments,and so ***,numerical methods have been widely utilized for obtaining the characteristics of self-oscillation including amplitude and ***,numerical methods are burdened by intricate computations and limited precision,hindering comprehensive investigations into self-oscillating *** this paper,the stability of a liquid crystal elastomer fiber self-oscillating system under a linear temperature field is studied,and analytical solutions for the amplitude and frequency are ***,we establish the governing equations of self-oscillation,elucidate two motion regimes,and reveal the underlying ***,we conduct a stability analysis and employ a multi-scale method to obtain the analytical solutions for the amplitude and *** results show agreement between the multi-scale and numerical *** research contributes to the examination of diverse self-oscillating systems and advances the theoretical analysis of self-oscillating systems rooted in active materials.

关键词： self-oscillation stability analysis multi-scale method liquid crystal elastomer linear temperature field

Breath-by-breath measurement of exhaled ammonia by acetonemodifier positive photoionization ion mobility spectrometry via online dilution and purging sampling

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Journal of Pharmaceutical Analysis 2023年第4期13卷 412-420页

作者： Lu Wang Dandan Jiang Lei Hua Chuang Chen Dongming li Weiguo Wang Yiqian Xu Qimu Yang haiyang li Song Leng CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Health Management Center The Second Affiliated Hospital of Dalian Medical UniversityDalian116023China University of Chinese Academy of Sciences Beijing100049China Dalian Key Laboratory for Online Analytical Instrumentation Dalian116023China Dalian Engineering Research Center of Breath Diagnostic Technology Dalian116023China

Exhaled ammonia(NH_(3))is an essential noninvasive biomarker for disease *** this study,an acetone-modifier positive photoionization ion mobility spectrometry(AM-PIMS)method was developed for accurate qualitative and quantitative analysis of exhaled NH_(3)with high selectivity and *** was introduced into the drift tube along with the drift gas as a modifier,and the characteristic NH_(3)product ion peak of(C_(3)H_(6)O)_(4)NH_(4)^(+)(K_(0)=1.45 cm^(2)/V·s)was obtained through the ion-molecule reaction with acetone reactant ions(C_(3)H_(6)O)_(2)H^(+)(K_(0)=1.87 cm^(2)/V·s),which significantly increased the peak-to-peak resolution and improved the accuracy of exhaled NH_(3)qualitative ***,the interference of high humidity and the memory effect of NH_(3)molecules were significantly reduced via online dilution and purging sampling,thus realizing breath-by-breath *** a result,a wide quantitative range of 5.87-140.92μmol/L with a response time of 40 ms was achieved,and the exhaled NH_(3)profile could be synchronized with the concentration curve of exhaled CO_(2).Finally,the analytical capacity of AM-PIMS was demonstrated by measuring the exhaled NH_(3)of healthy subjects,demonstrating its great potential for clinical disease diagnosis.

关键词： Photoionization ion mobility spectrometry Acetone modifier Exhaled ammonia Breath analysis

Sequence optimization for multiple asteroids rendezvous via cluster analysis and probability-based beam search

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Science China(Technological Sciences) 2021年第1期64卷 122-130页

作者： li haiyang BAOYIN HeXi School of Aerospace Engineering Tsinghua UniversityBeijing 100084China

It is of great significance to visit multiple asteroids in a space *** this paper,the multiple asteroids mission optimization is implemented using cluster analysis and probability-based beam *** is performed to select the first asteroid to *** cluster algorithms are investigated and affinity propagation is *** four beam search algorithms that are deterministic beam search and three probability-based beam search variants,probabilistic beam search,ant-colony beam search,and evolving beam search,are applied to search for the rendezvous *** beam search as a heuristic tree search algorithm is widely applied in multitarget sequence optimization,but it has an obvious drawback of the conflict between the number of pruned nodes and the possibility of finding optimal solutions,which can be improved by probability-based beam *** three probability-based beam search,the ant-colony beam search has a learning mechanism,and evolving beam search is constructed based on ant-colony beam search and has an evolutionary *** show that the introduction of randomness can improve beam search,and beam search variants with the learning and evolutionary mechanism have an excellent performance.

关键词： interplanetary trajectory optimization multi-target mission cluster analysis probability-based beam search

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循环肿瘤DNA在结直肠癌中的应用

中华全科医学 2024年第3期22卷 368-371页

作者：伍龙吴欢黄飞李晓云甄运寰李海洋贵州医科大学附属医院肛肠外科贵州贵阳550004 贵州医科大学附属医院感染科贵州医科大学附属医院肝胆外科

结直肠癌是我国常见的癌症类型之一,也是世界第二大死因。随着诊断技术的发展和包括手术和化疗在内的治疗方法的进步,其预后得到了极大的改善,但是仍有一定比例的患者在接受根治性治疗后出现复发。循环肿瘤DNA(ctDNA)是来自肿瘤细胞的... 详细信息

结直肠癌是我国常见的癌症类型之一,也是世界第二大死因。随着诊断技术的发展和包括手术和化疗在内的治疗方法的进步,其预后得到了极大的改善,但是仍有一定比例的患者在接受根治性治疗后出现复发。循环肿瘤DNA(ctDNA)是来自肿瘤细胞的循环游离DNA(cfDNA)片段,是一种新型的生物标志物,可以用于监测结直肠癌患者的放化疗效果和术后复发情况。与传统的生物标志物相比,ctDNA具有更高的特异度和灵敏度,并且可以在患者的血清中稳定存在。通过检测ctDNA的水平可以对患者的病情进行更加准确的评估,并根据检测结果制定更加个性化的治疗方案。在结直肠癌的诊疗过程中,ctDNA具有较高的特异度和灵敏度,通过检测患者的血清ctDNA水平,可以较早期判断患者是否可能患有结直肠癌。同时,通过比较患者治疗前后的血清ctDNA水平变化,可以评估患者的治疗效果。在结直肠癌的随访中,ctDNA同样具有很高的特异度和灵敏度,通过检测患者的血清ctDNA水平,可以有效地判断患者的复发情况。若患者血清ctDNA水平持续升高,则表明患者可能出现了复发,需要及时进行进一步的检查和干预。总之,ctDNA是一种新型的生物标志物,具有较高的特异度和灵敏度,可以用于监测结直肠癌患者的放化疗效果和术后复发情况,目前被认为是液体活检中的新型生物标志物之一,可作为放化疗效果评估、术后复发监测等的生物标志物。本文综述了ctDNA的特征及其在结直肠癌诊断、治疗和随访中的应用。

关键词：结直肠癌循环肿瘤DNA 应用

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复配黄原酸盐工艺研究

辽宁化工 2024年第6期53卷 943-945,949页

作者：李海洋吴庆伟仵亚妮高延雄金堆城钼业集团有限公司陕西渭南714100

以二硫化碳自溶剂法合成固体黄药,通过探索在原料、浆料和成品阶段的复配研究,合成出具有最优选矿效果的乙基钠、异丁基钠复配黄原酸盐。其工艺为在成品阶段进行复配,m(C_(2)H_(5)OH)∶m(C_(4)H_(9)OH)=1∶9,浮选出的硫精矿品位为48.64%... 详细信息

以二硫化碳自溶剂法合成固体黄药,通过探索在原料、浆料和成品阶段的复配研究,合成出具有最优选矿效果的乙基钠、异丁基钠复配黄原酸盐。其工艺为在成品阶段进行复配,m(C_(2)H_(5)OH)∶m(C_(4)H_(9)OH)=1∶9,浮选出的硫精矿品位为48.64%,回收率为74.71%,在选矿药剂定制化方面有较好的应用前景。

关键词：复配黄药选矿自溶剂法合成

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