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IL-17B/IL-17RB signaling regulates lysine 63-linked Beclin-1 ubiquitination to strengthen self-renewal and tumorigenesis in gastric cancer

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IL-17B/IL-17RB signaling regulates lysine 63-linked Beclin-1...

Therapeutic Uses of Lycium barbarum Polysaccharides on Idiopathic Thrombocytopenic Purpura Mice via Inhibition of Oxidative Stress

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Qingli Bie haixin dong Bin Zhang Department of Laboratory Medicine Affiliated Hospital of Jining Medical University Institute of Forensic Medicine and Laboratory Medicine Jining Medical University

Cancer stem cells(CSCs) display many malignant biological traits including tumorigenesis, metastasis, drug resistance and angiogenesis. Discovering new markers specific for CSCs and elucidating their regulatory mechanisms is a significant goal. Herein, we found that IL-17 B/IL-17 RB signaling promoted self-renewal and tumorigenesis of gastric cancer(GC) cells by activating autophagy. We identified this pathway by first determining that IL-17 RB expression is significantly upregulated in spheroid cells, which was closely associated with the degree of differentiation of patient-derived GC tissues. Exogenous recombinant IL-17 B(rIL-17 B) promoted the self-renewal capacity of GC cells in vitro and enhanced tumor growth and metastasis in vivo. Moreover, we found that rIL-17 B induced autophagosome formation and cleavage-mediated transformation of LC3 in GC and 293 T cells. Interestingly, inhibition of autophagy by ATG7 knockdown reversed rIL-17 B induced self-renewal of GC cells. Further study revealed that rIL-17 B promoted K63-linked ubiquitination of beclin-1 by mediating the binding of TRAF6 to beclin-1. Interfering with IL-17 RB expression abolished all the effects of rIL-17 B on ubiquitination of beclin-1 and autophagic activation of GC cells. Lastly, we discovered that IL-17 B expression in the serum of patients was positively correlated with IL-17 RB expression in GC tissues. In addition, rIL-17 B increased IL-17 RB expression in GC cells. Direct overexpression of IL-17 RB in 293 T cells mimics stimulated rIL-17 B, which promoted K63-linked ubiquitination of beclin-1 and binding of TRAF6 to beclin-1. Together, these results revealed the novel action of IL-17 B/IL-17 RB signaling on CSCs and might provide new therapeutic targets against gastric cancer.

关键词： IL-17B IL-17RB autophagy K63-linked ubiquitination of Beclin-1 gastric cancer cancer stem cell

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Journal of Biosciences and Medicines 2016年第8期4卷 17-23页

作者： Chengqiang Jin haixin dong Jianwei Zhou Shuhua Lu Xiaxia Yang Zhenwen Qian Yanxia Jia Analytical Section of Attached Hospital of Jining Medical College Jining China Radiology Department of Jining No. 1 People’s Hospital Jining China

The aim of the present study was to investigate the effect of Lycium barbarum polysaccharides (LBP) on the treatment of ITP mice and to explore its mechanism. Forty idiopathic thrombocytopenic purpura (ITP) mice were divided randomly into a model control group and LBP groups I, II, III and IV. ITP mice in LBP groups I, II, III, and IV were administered LBP at four different doses (50, 100, 200 and 400 mg·kg-1·d-1, respectively) for 7 days by gavage. Blood samples were collected from the tail veins of the mice after treatment. Platelet counts were determined, and the total antioxidant status (TAS), total oxidant status (TOS) were measured with ELISA kits. The platelet count was (30.28 ± 13.42) × 109/L in the model control group, and the number of platelets in all LBP groups was higher than that in the model control group. The platelet count increased, and it reached (67.09 ± 10.81) × 109/L in LBP group I;the platelet counts in the other three groups increased significantly compared to LBP group I, and they did not differ significantly. TAS concentrations in the LBP groups were significantly increased compared to the model control group, whereas TOS concentrations were significantly decreased. Taken together, these results indicate that LBP is effective at increasing the number of platelet (PLT), and LBP may treat ITP mice via suppressing oxidative stress.

关键词： Lycium barbarum Polysaccharides Idiopathic Thrombocytopenic Purpura Total Antioxidant Status Total Oxidant Status

Pt/TiO_2催化剂微乳液法制备条件对邻氯硝基苯选择性加氢性能的影响

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石油炼制与化工 2012年第4期43卷 30-33页

作者：宋华董海鑫李锋汪淑影东北石油大学化学化工学院黑龙江大庆163318 同煤广发化学工业有限公司

采用十六烷基三甲基溴化铵/正丁醇/环己烷/H2PtCl6溶液的微乳体系,以N2H4.H2O为还原剂、TiO2为载体制备Pt/TiO2催化剂。以邻氯硝基苯选择性加氢反应为探针反应,考察微乳液组成及TiO2晶型对催化剂选择性加氢活性的影响。结果表明:当微乳... 详细信息

采用十六烷基三甲基溴化铵/正丁醇/环己烷/H2PtCl6溶液的微乳体系,以N2H4.H2O为还原剂、TiO2为载体制备Pt/TiO2催化剂。以邻氯硝基苯选择性加氢反应为探针反应,考察微乳液组成及TiO2晶型对催化剂选择性加氢活性的影响。结果表明:当微乳体系中mCTAB∶m正丁醇=3∶7、(mCTAB+m正丁醇)∶m环己烷=3∶7、H2PtCl6溶液用量占体系质量的3.6%时,制备的Pt/TiO2催化剂的邻氯硝基苯选择性加氢活性最高;锐钛矿型TiO2有助于提高催化剂的活性。

关键词：微乳液 Pt/TiO2 邻氯硝基苯选择性加氢

<i>Cedecea lapagei</i>in a Patient with Multiple Injuries: Report of a Rare Case

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Journal of Biosciences and Medicines 2021年第11期9卷 1-5页

作者： Xiaofan Xu Kaiyue Chang dongxue Song Guangtong Tian Xiaohang Hu Liqing Jiang Bin Zhang Qingli Bie Shuhua Lu Xiaozhe Li haixin dong Chengqiang Jin School of Clinical Medicine Jining Medical University Jining China Department of Clinical Laboratory Jining Hospital of traditional Chinese Medicine Jining China Department of Clinical Laboratory The Affiliated Hospital of Jining Medical University Jining China

Cedecea lapagei (C. lapagei) is an opportunistic pathogen in old patients with many comorbid diseases and the immunosuppressed. It is a gram-negative, facultative anaerobe bacterium of the Enterobacteriaceae family. We present a rare case of a patient with multiple injuries, C. lapagei was found from the exudate of the wound, what’s more, the Proteus vulgaris was also found in blood culture medium at the same time. According to the available literature, this is the first case of simultaneous infection of two bacteria including C. lapagei and Proteus vulgaris from the exudate of the traumatic wound.

关键词： C. lapagei Cedecea Proteus vulgaris Exudate

Detection of TCRβchain CDR3 spectratyping in the peripheral blood and tissue of patients with colorectal carcinoma with RQ-PCR with DNA melting curve technique

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Detection of TCRβchain CDR3 spectratyping in the peripheral...

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Jianwei Zhou~(,2),haixin dong,Cui Kong,Yuanmin g Zhao,Xinsheng Yao 1 The Affiliated Hospital of Jining Medical College 2 Zunyi Medical College

Objective To primarily explore CDR3 spectratyping and molecular features of TCR |3 chain in the peripheral blood and tissue of patients with colorectal carcinoma with RQ-PCR with DNA melting curve technique. MethodsBlood and tissue samples were collected from seven patients with colorectal carcinoma (CRC),while blood samples were also collected from two healthy controls as *** the

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血浆脂多糖检测与血液细菌培养结果关系探讨

医学检验与临床 2010年第4期21卷 11-12页

作者：董海新曹震济宁医学院附属医院检验科山东济宁272029

目的探讨脂多糖检测与血液细菌培养的相关性及其意义,以期为临床诊断选择更为合理有效的辅助检查提供参考.方法回顾性分析我院37例脂多糖与血培养同时检测的患者病例,并对结果进行统计分析.结果血培养阳性组血脂多糖含量明显高于血... 详细信息

目的探讨脂多糖检测与血液细菌培养的相关性及其意义,以期为临床诊断选择更为合理有效的辅助检查提供参考.方法回顾性分析我院37例脂多糖与血培养同时检测的患者病例,并对结果进行统计分析.结果血培养阳性组血脂多糖含量明显高于血培养阴性组脂多糖含量[(33.66±8.42)pg/ml VS(1.90±1.66)pg/ml,t=8.413,P0.05].结论脂多糖检测是对革兰阴性菌感染早期诊断的重要的有效的快速辅助诊断指标,血培养阴性患者白细胞数量不能反应脂多糖含量的变化情况.

关键词：脂多糖血培养革兰阴性菌白细胞

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脂多糖检测对疾病的诊断与治疗的意义

医学检验与临床 2010年第5期21卷 100-101,96页

作者：董海新曹震济宁医学院附属医院检验科山东济宁272029 济宁医学院医学检验系05级实习生山东济宁272111

脂多糖是细菌内毒素的主要成分,主要存在于革兰阴性菌菌体的细胞壁内,极少数存在于革兰阳性杆菌、真菌、支原体中.脂多糖一般在细菌死后细胞壁崩解时释出,活菌亦可以发疱形式将内毒素释出.其化学成分包括脂质A、核心多糖和O抗原3个组成... 详细信息

脂多糖是细菌内毒素的主要成分,主要存在于革兰阴性菌菌体的细胞壁内,极少数存在于革兰阳性杆菌、真菌、支原体中.脂多糖一般在细菌死后细胞壁崩解时释出,活菌亦可以发疱形式将内毒素释出.其化学成分包括脂质A、核心多糖和O抗原3个组成部分.既可对机体产生许多致病作用,又可对机体发挥有益的生物学活性.其主要检测方法有比色法、凝胶法、浊度法、酶联免疫吸附法、流式细胞仪法.脂多糖的检测对消化系统疾病、细菌感染性疾病、

关键词：消化系统疾病脂多糖检测细菌内毒素酶联免疫吸附法细菌感染性疾病治疗诊断

羟考酮对乳腺癌手术患者麻醉效果及对S100β、BDNF、Aβ-42/tau水平的影响

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当代医学 2021年第25期27卷 35-36页

作者：董海鑫黄斌侯磊沈阳医学院附属中心医院麻醉科辽宁沈阳110024

目的研究羟考酮对乳腺癌手术患者麻醉效果及对S100β、BDNF、Aβ-42/tau水平的影响。方法选取2019年1—12月本院收治的乳腺癌手术患者90例,按随机数字法分为参照组和研究组,各45例。参照组采用舒芬太尼全麻,研究组采用羟考酮全麻。比较... 详细信息

目的研究羟考酮对乳腺癌手术患者麻醉效果及对S100β、BDNF、Aβ-42/tau水平的影响。方法选取2019年1—12月本院收治的乳腺癌手术患者90例,按随机数字法分为参照组和研究组,各45例。参照组采用舒芬太尼全麻,研究组采用羟考酮全麻。比较两组术后各时段疼痛情况、手术前后S100β、BDNF、Aβ-42/tau水平变化以及不良反应发生率。结果术前,两组血清S100β、BDNF和Aβ-42/tau水平比较差异无统计学意义;研究组术后2、6、12、24 h疼痛评分均低于参照组,术后24 h血清S100β低于参照组,BDNF和Aβ-42/tau水平均高于参照组,差异有统计学意义(P<0.05)。两组不良反应发生率比较差异无统计学意义。结论羟考酮应用于乳腺癌手术患者镇痛效果显著,可有效改善术后血清S100β、BDNF、Aβ-42tau水平,进而避免或减少认知功能障碍发生,且安全性较高,值得临床推广应用。

关键词：羟考酮舒芬太尼乳腺癌麻醉效果

超声引导股神经阻滞与髂筋膜腔隙阻滞在全膝关节置换术后镇痛的效果对比

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当代医学 2021年第26期27卷 33-35页

作者：董海鑫黄斌侯磊沈阳医学院附属中心医院麻醉科辽宁沈阳110024

目的比较超声引导股神经阻滞与髂筋膜腔隙阻滞在全膝关节置换术后镇痛的效果。方法选取2019年7月至2020年6月本院收治的行全膝关节置换术患者86例,随机分为参照组与研究组,各43例。参照组给予超声引导股神经阻滞镇痛,研究组给予超声引... 详细信息

目的比较超声引导股神经阻滞与髂筋膜腔隙阻滞在全膝关节置换术后镇痛的效果。方法选取2019年7月至2020年6月本院收治的行全膝关节置换术患者86例,随机分为参照组与研究组,各43例。参照组给予超声引导股神经阻滞镇痛,研究组给予超声引导髂筋膜腔隙阻滞镇痛。比较两组穿刺注药时间、术后苏醒时间、有效镇痛时间、芬太尼总用量、术后24 h患肢主动关节屈曲角、术后各时间段静息和活动时疼痛以及不良反应发生情况。结果两组患者苏醒时间及术后6、12、24 h静息VAS评分比较差异无统计学意义。研究组患者穿刺注药时间短于参照组,有效镇痛时间长于参照组,芬太尼总用量少于参照组,术后24 h患肢主动关节屈曲角大于参照组;术后6、12、24 h,研究组活动时VAS评分均明显低于参照组,不良反应发生率低于参照组,差异均有统计学意义(P<0.05)。结论在全膝关节置换术中给予超声引导髂筋膜腔隙阻滞镇痛效果优于神经阻滞,有利于缩短穿刺注药时间,减少芬太尼用量,延长镇痛时间,减少不良反应,从而减轻患者术后早期活动痛苦,促进患肢功能活动,值得临床推广应用。

关键词：超声引导股神经阻滞全膝关节置换镇痛