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A fluorogenic-inhibitor-based probe for profiling and imaging of monoamine oxidase A in live human glioma cells and clinical tissues

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Science China Chemistry 2023年第7期66卷 2053-2061页

作者： haixiao fang Panpan Li Congzhen Shen fang Tang Aixiang Ding Hua Bai Bo Peng Xuekang Yang Zhengqiu Li Kai Huang Sijun Pan Lin Li Wei Huang The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Future Display Institute in Xiamen Xiamen 361005China Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China School of Pharmacy Jinan UniversityGuangzhou 510632China Xijing Hospital The Fourth Military Medical UniversityXi’an 710032China

Monoamine oxidase A(MAO-A)plays a critical role in the development of glioma and other neurological disorders.Specific analysis of MAO-A activities and its drug interactions in intact tissue is important for biological and pharmacological research,but highly challenging with current chemical tools.Fluorogenic-inhibitor-based probes offer improved selectivity,sensitivity,and effectiveness to image and profile endogenous targets in an activity-based manner from mammalian cells,which are however rare.Herein,we report HD1 as the first fluorogenic-inhibitor-based probe that can selectively label endogenous MAO-A from various mammalian cells and clinical tissues.The probe was delicately designed based on N-propargyl tetrahydropyridine,a small MAO-A-specific fluorogenic and inhibitory war-head,so that the probe becomes fluorescent upon in situ enzymatic oxidation and covalent labeling of MAO-A.With the excellent binding affinity(in vitro K_(i)=285 n M)and fluorogenic properties,HD1 offers a promising approach to simultaneously image endogenous MAO-A activities by super-resolution fluorescence microscopy and study its drug interactions by subsequent activity-based protein profiling,in both live cells and human glioma tissues.

关键词： monoamine oxidase A fluorogenic-inhibitor-based probe super-resolution imaging activity-based protein profiling glioma

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Recent progress in two-photon small molecule fluorescent probes for enzymes

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Chinese Chemical Letters 2019年第10期30卷 1738-1744页

作者： Ding Chen Wenjing Qin haixiao fang Lan Wang Bo Peng Lin Li Wei Huang Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China Shaanxi Institute of Flexible Electronics(SIFE) Northwestern Polytechnical University(NPU)Xi'an 710072China

Enzymes are macromolecular biological catalysts which can accelerate chemical reactions in living organisms. Almost all the physiological metabolism activities in the cell need enzymes to sustain life via rapid catalysis. Currently, medical research has proved that abnormal enzyme activity is associated with numerous diseases, such as Parkinson’s disease(PD), Alzheimer’s disease(AD) and cancers. On the other hand, early diagnosis of those diseases is of great significance to improve the survival rate and cure rate.In the current diagnostic tools, two-photon fluorescent probes(TPFPs) are developing rapidly due to their unique advantages, such as higher spatial resolution, deeper imaging depth, and lower biotoxicity.Therefore, the design and synthesis of two-photon(TP) small molecule enzymatic probes have broad prospects for early diagnosis and treatment of diseases. As of now, scientists have developed many TP small molecule enzymatic probes. This review aims to summarize the TP small molecule enzymatic probes and expound the reaction mechanism.

关键词： Enzymes Fluorescence Two-photon probes Fluorogenic probes Small molecule Disease

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PtNiCu nanowires with advantageous lattice-plane boundary for enhanced ethanol electrooxidation

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Nano Research 2022年第4期15卷 2877-2886页

作者： Shuna Li Yao Wang Yunrui Li Xu fang Yujie Liu Mingxuan Li Zhen Wang Yufeng Gao haixiao Sun Fei Gao Xin Zhang Xiaoping Dai State Key Laboratory of Heavy Oil Processing College of Chemical Engineering and EnvironmentChina University of PetroleumBeijing 102249China Department of Chemistry Tsinghua UniversityBeijing 100084China Jiangsu Key Laboratory of Vehicle Emissions Control School of the EnvironmentCenter of Modern AnalysisKey Laboratory of Mesoscopic Chemistry of Ministry of Education of ChinaSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210093China

It is generally accepted that the interface effect and surface electronic structure of catalysts have vital impact on catalytic properties.Understanding and tailoring the atomic arrangement of interface structure are of great importance for electrocatalysis.Herein,we proposed a simple method to synthesize etching-PtNiCu nanowires(e-PtNiCu NWs)enclosed by both(110)and(100)facets evolving from PtNiCu nanowires(PtNiCu NWs)mainly with(111)facets by selectively etching process.After acetic acid etching treatment,the e-PtNiCu NWs possess high total proportions(88.3%)of(110)and(100)facets,whereas the(111)facet is dominant in PtNiCu NWs(64%)by qualitatively and quantitatively evaluation.Combining the structure characterizations and performance tests of ethanol electrooxidation reaction(EOR),we find that the e-PtNiCu NWs display remarkably performance for EOR,which is nearly 4.5 times and 1.5 times enhancement compared with the state-of-the-art Pt/C catalyst,as well as 2.2 and 1.4 times of PtNiCu NWs,in specific activity and mass activity,respectively.The improved performance of e-PtNiCu NWs is attributed to synergistic catalytic effect between(110)and(100)facets that not only significantly decreases the onset potentials of adsorbed CO(CO_(ads))but also favors the oxidation of CO_(ads)on the surface of catalyst.Furthermore,thermodynamics and kinetic studies indicate that the synergistic effect of both(110)and(100)facets in e-PtNiCu NWs can decrease the activation energy barrier and facilitate the charge transfer during the reaction.This work provides a promising approach to construct catalysts with tunable surface electronic structure towards efficient electrocatalysis.

关键词： chemical etching crystal-facet engineering synergistic effect ethanol electrooxidation CO resistibility

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Interfacial synergistic effect in SnO_(2)/PtNi nanocrystals enclosed by high-index facets for high-efficiency ethylene glycol electrooxidation

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Nano Research 2022年第9期15卷 7877-7886页

作者： Shuna Li haixiao Sun Jiaai Zhang Longjiao Zheng Yunrui Li Xu fang Yujie Liu Qi Song Zhen Wang Yufeng Gao Xin Zhang Xiaoping Dai Yandi Cai Fei Gao State Key Laboratory of Heavy Oil Processing College of Chemical Engineering and EnvironmentChina University of PetroleumBeijing 102249China Jiangsu Key Laboratory of Vehicle Emissions Control School of the EnvironmentNanjing UniversityNanjing 210093China

Strengthening the oxide-metal interfacial synergistic interaction in nanocatalysts is identified as potential strategy to boost intrinsic activities and the availability of active sites by regulating the surface/interface environment of catalysts.Herein,the SnO_(2)/PtNi concave nanocubes(CNCs)enclosed by high-index facets(HIFs)with tunable SnO_(2)composition are successfully fabricated through combining the hydrothermal and self-assembly method.The interfacial interaction between ultrafine SnO_(2)nanoparticles and PtNi with HIFs surface structure is characterized by analytical techniques.The as-prepared 0.20%SnO_(2)/PtNi catalyst exhibits extraordinarily high catalytic performance for ethylene glycol electrooxidation(EGOR)in acidic conditions with specific activity of 3.06 mA/cm^(2),which represents 6.2-fold enhancement compared with the state-of-the-art Pt/C catalyst.Additionally,the kinetic study demonstrates that the strong interfacial interaction between SnO_(2)and PtNi not only degrades the activation energy barrier during the process of EGOR but also enhances the CO-resistance ability and long-term stability.This study provides a novel perspective to construct highly efficient and stable electrocatalysts for energy conversions.

关键词： Pt-based catalyst high-index facets undercoordinated surface atoms interfacial synergistic effect ethylene glycol electrooxidation

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Rational Design of Two-photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Live Cells and Tissues

Rational Design of Two-photon Fluorogenic Probe for Visualiz...

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第十一届全国化学生物学学术会议

作者： haixiao fang Hang Zhang Lin Li Chengwu Zhang Qiong Wu Shao Q.Yao Wei Huang Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Nanjing Tech University (NanjingTech)

Monoamine oxidases(MAOs) are important mammalian enzymes that catalyze the transformation of various neurotransmitters including serotonin,dopamine,phenethylamine,and others. They are normally expressed on the outer membrane of mitochondria in cells.In human,MAOs have been found to play key roles in maintaining the homeostasis of neurotransmission.At present,there are two known human MAOs(i.e.MAO-A and MAO-B),which,despite high sequence homology(～70%),differ significantly in their biological roles,cell distributions and substrate specificity. The ability to differentiate them by using fluorescence etection/imaging technology is of significant biological relevance,but highly challenging with available chemical tools.Herein,we report the first MAO-A specific two-photon fluorogenic probe(F1),capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of different biological samples,including MAO-A expressing neuronal SY-SY5Y cells and the brain of tumor-bearing mice,by using two-photon fluorescence microscopy(TPFM) without toxicity at working concentrations.

关键词： monoamine oxidase A MPTP two photon fluorogenic probes molecular docking

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基于虚拟现实技术的辽宁舰仿真体验系统设计

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信息与电脑 2022年第3期34卷 128-130页

作者：方妹娟宫海晓陈琳梧州学院大数据与软件工程学院广西梧州543001

为了提升舰船仿真体验系统的实际应用范围与效果,并完善系统的内部结构,设计基于虚拟现实技术的辽宁舰仿真体验系统。通过物理模块和深度双向循环运行电路的创建来完成硬件设计。进行VR交互程序仿真设计,同时采用虚拟现实技术下发显控... 详细信息

为了提升舰船仿真体验系统的实际应用范围与效果,并完善系统的内部结构,设计基于虚拟现实技术的辽宁舰仿真体验系统。通过物理模块和深度双向循环运行电路的创建来完成硬件设计。进行VR交互程序仿真设计,同时采用虚拟现实技术下发显控仿真指令,完成软件的设计。最终的系统测试结果表明,对于未应用虚拟现实技术的辽宁舰仿真体验系统测试组,本文所设计的系统最终完成的指令数量最高可以达到55条,表明此系统的执行精准度较高、控制范围较为广泛,并且仿真体验过程中出现的执行误差几率也较低,具有较好的实际应用价值。

关键词：虚拟现实技术仿真体验系统显控仿真指令

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液态发酵红曲黄色素粗提物色素组分的分离纯化与鉴定

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食品与发酵工业 2020年第1期46卷 91-98页

作者：徐菲菲徐海笑梁蓉钟芳 (江南大学食品学院江苏无锡214122 江南大学化学与材料工程学院江苏无锡214122

采用高效液相色谱、质谱和核磁共振分析,对液态发酵红曲黄色素粗提物色素组分进行分离纯化和分子结构鉴定,确定其化学结构式。结果表明,红曲黄色素有2种主要色素成分,组分A和组分B,两者在分析型液相色谱上分离度达到2.51。进一步将分析... 详细信息

采用高效液相色谱、质谱和核磁共振分析,对液态发酵红曲黄色素粗提物色素组分进行分离纯化和分子结构鉴定,确定其化学结构式。结果表明,红曲黄色素有2种主要色素成分,组分A和组分B,两者在分析型液相色谱上分离度达到2.51。进一步将分析型液相分离条件在制备型液相上进行放大并调整,收集得到组分A和组分B,纯度分别为98.95%和99.02%,达到标准品的纯度。通过质谱分析结合一维及二维核磁共振,确定组分A的相对分子质量为358,为红曲素(C21H26O5),组分B的相对分子质量为332,为Monaphilone B(C20H28O4),A和B的显色性能来自于其分子结构中的α,β-不饱和酮结构。

关键词：红曲黄色素色素分离纯化高效液相色谱质谱核磁共振

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夹竹桃科DNA条形码的初步筛选

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中华中医药学刊 2022年第1期40卷 93-96,I0021页

作者：方振名赵仕花卢海啸温玉婷梁津慧叶晓霞玉林师范学院广西玉林537000 玉林师范学院地产药用资源开发与生物工程中心广西玉林537000

DNA条形码(DNA barcoding)是一种新的物种鉴定技术,COI序列已成功应用于动物的鉴定,但目前国际上还没有找到一个通用序列能鉴定所有植物物种。为探索适合于夹竹桃科植物DNA条形码序列,拟用matK、ITS2、ITS 3个常规引物序列对夹竹桃科5... 详细信息

DNA条形码(DNA barcoding)是一种新的物种鉴定技术,COI序列已成功应用于动物的鉴定,但目前国际上还没有找到一个通用序列能鉴定所有植物物种。为探索适合于夹竹桃科植物DNA条形码序列,拟用matK、ITS2、ITS 3个常规引物序列对夹竹桃科5个属的部分植物进行基因序列对比分析,并运用MEGA 7.0构建系统进化树进行系统发育分析、鉴定其亲缘关系。结果显示在ITS2、ITS、matK 3个引物序列中,只有matK引物序列能正常扩增目的基因,扩增成功率达到100%,并且该引物序列对比分析和系统发育树的聚类结果与传统分类学的鉴定结果一致。建议将matK序列作为夹竹桃科DNA条形码鉴定候选序列之一。

关键词：夹竹桃科 DNA条形码分子鉴定 matK

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单胺氧化酶A/B亚型的活性检测在神经系统疾病诊疗中的应用

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中国临床药理学与治疗学 2020年第9期25卷 1052-1058页

作者：王莉敏方斌方海啸柏桦李林西北工业大学柔性电子研究院&柔性电子前沿科学中心&生物医学材料与工程研究院陕西西安710072 南京工业大学先进材料研究院江苏南京211816

分布于线粒体外膜上的单胺氧化酶(monoamine oxidase,MAOs)表达异常会引起神经递质传递功能紊乱,与精神性和神经退行性疾病有着密切联系。因此,对于MAOs(MAO-A/B)活性的特异性检测和调控将有助于神经系统相关疾病的诊断和治疗;利用特异... 详细信息

分布于线粒体外膜上的单胺氧化酶(monoamine oxidase,MAOs)表达异常会引起神经递质传递功能紊乱,与精神性和神经退行性疾病有着密切联系。因此,对于MAOs(MAO-A/B)活性的特异性检测和调控将有助于神经系统相关疾病的诊断和治疗;利用特异性强、灵敏度高的荧光检测/成像技术区分MAO-A/B两种亚型具有重要的生物学意义。本课题组基于“空间构型转换”的概念,设计特异性荧光“Switch-ON”行为的双光子小分子荧光探针U1和F1,结合双光子荧光显微成像技术在细胞和组织层面上分别实现对内源性MAO-B和MAO-A的特异性活性检测,旨在为进一步理解MAOs的生理作用,开发MAOs抑制剂创新药物及神经系统疾病的诊疗提供帮助。

关键词：神经系统疾病单胺氧化酶荧光探针活性检测

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沉管技术在广西输气管道河流穿越中的应用

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天然气与石油 2020年第4期38卷 19-23页

作者：房海晓孙禹广西天然气管道有限责任公司广西北海536000

沉管技术在输气管道施工应用中已越来越普遍。广西输气管道工程中,九洲江、郁江和红水河穿越工程根据现场踏勘均采用沉管技术穿越。结合工程实例,通过自然条件的对比,论述了沉管穿越施工中管道预制方法、漂管方式、沉管方式、配重方式... 详细信息

沉管技术在输气管道施工应用中已越来越普遍。广西输气管道工程中,九洲江、郁江和红水河穿越工程根据现场踏勘均采用沉管技术穿越。结合工程实例,通过自然条件的对比,论述了沉管穿越施工中管道预制方法、漂管方式、沉管方式、配重方式、沟底回填和护岸水工保护等方面的技术。三大中型条河流在地形地貌、水下地质条件、水文条件等自然条件上均有很大差异,因此沉管施工方案也各有不同,具有很强的针对性可为同类工程施工提供借鉴。

关键词：管道穿越工程沉管技术方案对比施工技术

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