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抗氧化型壳聚糖/大豆蛋白复合食用膜的制备与应用

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食品工业科技 2024年第6期45卷 210-218页

作者：杨欧张晓湘徐小涵孙玥梁进李雪玲李梅青张海伟安徽农业大学茶与食品科技学院农业农村部江淮农产品精深加工与资源利用重点实验室安徽省特色农产品高值化利用工程研究中心安徽合肥230036

以壳聚糖和大豆分离蛋白为复合膜基材,天然抗氧化剂为活性物质,制备具有抑制脂质氧化且可食用的活性保鲜膜。通过膜的机械性能、微观结构、物理性质与抗氧化性能,优化添加抗氧化剂的种类及浓度,并分析复合膜对核桃油贮藏保质效果。结果... 详细信息

以壳聚糖和大豆分离蛋白为复合膜基材,天然抗氧化剂为活性物质,制备具有抑制脂质氧化且可食用的活性保鲜膜。通过膜的机械性能、微观结构、物理性质与抗氧化性能,优化添加抗氧化剂的种类及浓度,并分析复合膜对核桃油贮藏保质效果。结果表明,8种天然抗氧化剂添加均显著提高了复合膜的阻氧性能(P<0.05),其中虾青素、葡萄籽提取物、维生素C添加后使得油脂过氧化值减少约80%,且保持良好的机械性能。当虾青素添加浓度为0.3%时,复合膜表现最佳性能,抗拉强度为6.546 MPa,断裂伸长率为69.962%,DPPH自由基清除能力为80.1%,水蒸气渗透率为1.21 g·mm/m^(2)·h·kPa;扫描电子显微镜显示膜表面平整光滑、规则、均匀;红外光谱分析显示成膜材料间均具有良好的相容性;差示扫描热量仪分析表明虾青素复合膜的热焓值最高,达到233.940 J/g,热稳定性最好。将含虾青素的复合膜包裹核桃油后,油脂过氧化值比对照降低81.8%,共轭二烯及共轭三烯值分别下降44.4%和66.4%。添加虾青素的壳聚糖/大豆蛋白复合膜能够显著延缓油脂氧化进程。

关键词：复合食用膜抗氧化虾青素核桃油

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壬醛与香芹酚复合抑菌微胶囊的制备及其对蓝莓的保鲜作用

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食品与发酵工业 2024年第9期50卷 238-245,I0004,I0005页

作者：戴瑶陈玥琰张翔王莉李梅青高学玲张海伟安徽农业大学茶与食品科技学院农业农村部江淮农产品精深加工与资源利用重点实验室安徽省农产品加工工程实验室安徽合肥230036

为开发适合蓝莓采后贮藏保鲜的非接触式抑菌型天然固体缓释保鲜剂,以蓝莓采后主要病害病原菌灰霉菌为目标菌种,首先采用棋盘法从10种抑菌精油中筛选出具有协同抑菌作用的精油组合,再以包埋率为指标,通过响应面实验优化复合精油-β-环糊... 详细信息

为开发适合蓝莓采后贮藏保鲜的非接触式抑菌型天然固体缓释保鲜剂,以蓝莓采后主要病害病原菌灰霉菌为目标菌种,首先采用棋盘法从10种抑菌精油中筛选出具有协同抑菌作用的精油组合,再以包埋率为指标,通过响应面实验优化复合精油-β-环糊精微胶囊最佳制备工艺条件,并对其结构及功能特性进行表征,最后研究了微胶囊对蓝莓的保鲜效果。结果表明,壬醛与香芹酚具有协同抑制灰霉菌生长的效果;最佳制备工艺为β-环糊精/复合精油比8.75∶1(g/mL),包埋温度50.3℃,包埋时间2.0 h,微胶囊包埋率可达78.23%;扫描电镜观察微胶囊形态为规则晶体结构,平均粒径为16.86μm;抑菌实验表明,随着微胶囊用量增加,其对灰霉菌的抑制作用也提高;经过26 d释放后,在4℃和20℃微胶囊中复合精油最大累积释放率分别为50.52%和57.78%,具备缓释特性。在蓝莓保鲜实验中,贮藏至2周+8 d,相较空白对照(CK),6%(以果实质量分数计)微胶囊组蓝莓的商品率提高了39.73%、霉菌总数降低了61.39%,延缓了硬度、可溶性固形物、可滴定酸与抗坏血酸含量的下降,有效保持了蓝莓的品质。

关键词：壬醛香芹酚协同抑菌:微胶囊蓝莓

德尔布有孢圆酵母与四种酿酒酵母顺序发酵对草莓酒香气与感官喜好性的影响

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食品与发酵工业 2024年第14期50卷 165-172,I0009,I0010页

作者：侯若婷赵鹏徐馨莹张笑笑王宇张海伟安徽农业大学茶与食品科技学院农业农村部江淮农产品精深加工与资源利用重点实验室安徽省特色农产品高值化利用工程研究中心安徽合肥230036

为获得香气浓郁、风味典型的草莓果酒,选用非酿酒酵母德尔布有孢圆酵母(Torulaspora delbrueckii,Td)分别与4种商业酿酒酵母(D254,K1,F15和Aq)以1∶1接种比例进行顺序共发酵,采用固相微萃取-气相色谱-质谱联用(solid-phase microextract... 详细信息

为获得香气浓郁、风味典型的草莓果酒,选用非酿酒酵母德尔布有孢圆酵母(Torulaspora delbrueckii,Td)分别与4种商业酿酒酵母(D254,K1,F15和Aq)以1∶1接种比例进行顺序共发酵,采用固相微萃取-气相色谱-质谱联用(solid-phase microextraction-gas chromatograph-mass spectrometer,SPME-GC-MS)技术与9点快感标度法等方法分析了4种混菌发酵组合对草莓酒的理化指标、挥发性香气物质及消费者喜好性的影响。结果表明,Td与不同酿酒酵母混合发酵对草莓果酒的色度与香气成分有显著性影响。Td与Aq、D254顺序接种发酵可使草莓果酒获得更高的红度和饱和度。在香气方面,4种草莓酒中共检测出67种挥发性物质,主要是酯类、醇类和酸类;通过韦恩图分析,4种酒样中共有香气组分19种,其中Td+D254组产生的香气化合物最多(48种)。主要呈香物质(ROAV>0.1)主成分分析表明,Td+K1组和Td+D254组产生的挥发性活性化合物相似,而且与辛酸、壬醛、S-(-)-2-甲基-1-丁醇等物质密切相关。消费者喜好度检验结果表明,Td+D254组草莓酒具有较强的果香、青爽味与花香,获得最高的喜好度6.7±1.2。综上,将非酿酒酵母Td与酿酒酵母D254顺序组合发酵具有为草莓果酒提高香气品质的应用潜力。

关键词：德尔布有孢圆酵母顺序共发酵草莓果酒挥发性物质

Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus

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Virologica Sinica 2021年第6期36卷 1600-1610页

作者： Rui Wang haiwei zhang Cheng Peng Jian Shi Huajun zhang Rui Gong CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Ebola virus(EBOV) belongs to the Filoviridae family and causes severe illnesses such as hemorrhagic fever with a high mortality rate up to 90%. Now two antibody drugs termed Inmazeb and Ebanga have been approved for treating EBOV infection. However, clinical studies have demonstrated that the mortality rate of the patients who received these two antibody drugs remains above 30%. Therefore, novel therapeutics with better efficacy is still desired. The isolated human IgG1 constant domain 2(CH2 domain) has been proposed as a scaffold for the development of C-based single domain antibodies(C-sd Abs) as therapeutic candidates against viral infections and other diseases. Here, we screened and identified a novel C-sd Ab termed M24 that targets EBOV glycoprotein(GP) from a C-sd Ab phage display library. M24 neutralizes the pseudotype EBOV with IC;of 0.8 nmol/L(12 ng/mL) and has modest neutralizing activity against authentic *** determination, including molecular docking and site mutation analysis, discloses that M24 binds to the internal fusion loop(IFL) within GP2, a transmembrane subunit of GP. Interestingly, we found that the binding of M24 to GP at pH5.5 has dramatically decreased compared to the binding at pH 7.5, which may lead to weak efficacy in the neutralization of authentic EBOV. Since no sd Ab against EBOV infection has been reported to date, our results not only give a proof of concept that sd Abs could be utilized for the development of potential therapeutic candidates against EBOV infection, but also provide useful information for the discovery and improvement of anti-EBOV agents.

关键词： Ebola virus(EBOV) Constant domain 2(CH2 domain) C-based single domain antibody(C-sdAb) Neutralize Fusion loop

Rapamycin promotes Schwann cell migration and nerve growth factor secretion

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Neural Regeneration Research 2014年第6期9卷 602-609页

作者： Fang Liu haiwei zhang Kaiming zhang Xinyu Wang Shipu Li Yixia Yin Department of Orthopedics Second Hospital of Yueyang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing and Biomedical Materials and Engineering Center Wuhan University of Technology

Rapamycin, similar to FKS06, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of raparnycin and FK506 on Sc hwann cells and investigated effects and mechanisms of rapamycin on improving peripheral nerve regeneration. Results demonstrated that the lowest rapamycin concentration （1.53 nmol/L） more significantly promoted Schwann cell migration than the highest FK506 concentration （100μmol/L）. Rapamycin promoted the secretion of nerve growth factors and upregulated growth-associated protein 43 expression in Schwann cells, but did not significantly affect Schwann cell proliferation. Therefore, rapamycin has potential application in peripheral nerve regeneration therapy.

关键词： nerve regeneration peripheral nerve injury rapamycin FK506 Schwann cell cell mi-gration nerve growth factor growth-associated protein 43 NSFC grant neural regeneration

Genotypic variation in phosphorus acquisition from sparingly soluble P sources is related to root morphology and root exudates in Brassica napus

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Science China(Life Sciences) 2011年第12期54卷 1134-1142页

作者： zhang haiwei HUANG Yu YE XiangSheng XU FangSen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China Key Laboratory of Subtropical Agriculture and Environment Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China

Genotypic variations in the adaptive response to low-phosphorus （P） stress and P-uptake efficiency have been widely reported in many crops. We conducted a pot experiment to evaluate the P-acquisition ability of two rapeseed （Brassica napus） geno- types supplied with two sparingly soluble sources of P, A1-P and Fe-P. Then, the root morphology, proton concentrations, and carboxylate content were investigated in a solution experiment to examine the genotypic difference in P-acquisition efficiency. Both genotypes produced greater biomass and accumulated more P when supplied with A1-P than when supplied with Fe-P. The P-efficient genotype 102 showed a significantly greater ability to deplete sparingly soluble P from the rhizosphere soil be- cause of its greater biomass and higher P uptake compared with those of the P-inefficient genotype 105. In the solution exper- iment, the P-efficient genotype under low-P conditions developed dominant root morphological traits, and it showed more in- tensive rhizosphere acidification because of greater H＋ effiux, higher H＋-ATPase activity, and greater exudation of carbox- ylates than the P-inefficient genotype. Thus, a combination of morphological and physiological mechanisms contributed to the genotypic variation in the utilization of different sparingly soluble P sources in B. napus.

关键词： Brassica napus sparingly soluble P genotypic variation root morphology H+ and carboxylate exudation

Chinese stalagmite paleoclimate researches: A review and perspective

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Science China Earth Sciences 2019年第10期62卷 1489-1513页

作者： Hai CHENG haiwei zhang Jingyao ZHAO Hanying LI Youfeng NING Gayatri KATHAYAT Institute of Global Environmental Change Xi’an Jiaotong University

Stalagmite is one kind of secondary carbonates formed in limestone caves(speleothem). After cave water droplets containing Ca2+and HCO3 drip onto floor, carbonate in the water might become supersaturated due to CO2 degassing under certain conditions, resulting in the formation of stalagmite in a process year after year. Stalagmite is one of important geological archives for paleoclimate research. The advantages include wide spatial distribution, suitable for U-Th and U-Pb dating, enriched in climate proxies, continuity, long time span, comparability and lower sampling cost etc. These factors have propelled stalagmite paleoclimate research to the forefront of global paleoclimatology with an irreplaceable role. The stalagmite paleoclimate study started in the western countries, mainly in Europe and America in 1960 s–1970 s, while the relevant research in China was progressively developed in the 1980 s–1990 s after the Reform and Opening up. Although there was a huge gap between the overall research level in China and western countries, a solid research foundation, as well as a number of talent teams were established during the period. In the 21 st century, starting from the publication of stalagmite records from Hulu Cave in Nanjing in 2001, the stalagmite paleoclimate research in China has ushered in a flourishing development and a real leap on the basis of international cooperation, resulting in significant international impacts. The landmark achievements, including establishment of the world’s longest(640000 years) East Asian monsoon stalagmite record, as well as the longest Indian monsoon(280000 years),South American monsoon(250000 years), North American westerly climate(330000 years), Central Asian westerly climate(135000 years), and northwestern China westerly climate(500000 years), have laid a milestone in the paleoclimate study in these climate domains. Importantly, these stalagmite records have revealed the relationship of Asian monsoon variations with solar

关键词： Cave stalagmites Oxygen isotope Asian monsoon Westerly Global climate change

ONE-PARAMETER FINITE DIFFERENCE METHODS AND THEIR ACCELERATED SCHEMES FOR SPACE-FRACTIONAL SINE-GORDON EQUATIONS WITH DISTRIBUTED DELAY

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Journal of Computational Mathematics 2024年第3期42卷 705-734页

作者： Tao Sun Chengjian zhang haiwei Sun School of Mathematics and Statistics Huazhong University of Science and TechnologyWuhan 430074China Hubei Key Laboratory of Engineering Modeling and Scientific Computing Huazhong University of Science and TechnologyWuhan 430074China Department of Mathematics University of MacaoMacaoChina

This paper deals with numerical methods for solving one-dimensional(1D)and twodimensional(2D)initial-boundary value problems(IBVPs)of space-fractional sine-Gordon equations(SGEs)with distributed *** 1D problems,we construct a kind of oneparameter finite difference(OPFD)*** is shown that,under a suitable condition,the proposed method is convergent with second order accuracy both in time and *** implementation,the preconditioned conjugate gradient(PCG)method with the Strang circulant preconditioner is carried out to improve the computational efficiency of the OPFD *** 2D problems,we develop another kind of OPFD *** such a method,two classes of accelerated schemes are suggested,one is alternative direction implicit(ADI)scheme and the other is ADI-PCG *** particular,we prove that ADI scheme can arrive at second-order accuracy in time and *** some numerical experiments,the computational effectiveness and accuracy of the methods are further ***,for the suggested methods,a numerical comparison in computational efficiency is presented.

关键词： Fractional sine-Gordon equation with distributed delay One-parameter finite difference methods Convergence analysis ADI scheme PCG method

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基于水流量目标的圆盘造球控制系统研究

冶金自动化 2024年第2期48卷 34-40页

作者：张学锋张海威祝忠阳余正伟龙红明冶金工程与资源综合利用安徽省重点实验室(安徽工业大学) 安徽工业大学冶金工程学院

针对球团矿生产过程中，球团粒度难以精确把控、生产方式较为落后等特点，提出了一种基于水流量目标的圆盘造球自动控制系统。首先，依托图像识别算法从实际生产过程中的球团图像里提取出球团的粒径大小、分布和个数等基本信息。然后，... 详细信息

针对球团矿生产过程中，球团粒度难以精确把控、生产方式较为落后等特点，提出了一种基于水流量目标的圆盘造球自动控制系统。首先，依托图像识别算法从实际生产过程中的球团图像里提取出球团的粒径大小、分布和个数等基本信息。然后，系统对接收到的球团信息进行分析计算，得到当前球团的生产状态与粒径变化趋势。最后，系统以球团粒径变化趋势和生产状态为参数确立水流量目标设定值，并且根据球团生产状态适当修改阀门开度调整周期以适应生产。系统实际运用于国内某钢铁厂圆盘造球机的控制，实践结果表明，在平均粒度与目标粒度的差值方面，自动控制模式相较于传统人工控制模式减少了32.54%的误差；在不同造球工况下，提出的控制系统在实际生产过程中水流量波动稳定，对比人工控制模式，球团粒度均方根误差减少了6.59%,具有良好的稳定性。这对提升球团生产效率及合格率，降低人工劳动强度有积极作用。

关键词：流量目标控制法圆盘造球机粒度检测图像识别 PLC 工业控制