检索结果-南通市图书馆

Science China Chemistry 2024年

作者： An Lin Jingyuan Liu Yougen Xu haiting wu Yating Chen Yan Zhang Lebin Su Xiaodan Zhao Kuangbiao Liao Institute of Organic Chemistry & MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of Chemistry Sun Yat-sen University Guangzhou National Laboratory Bioland Laboratory

Herein, we report a novel and highly efficient method for the synthesis of α-phosphoryloxy carbonyl compounds via Rucatalyzed P(O)O–H insertion reactions of sulfoxonium ylides and phosphinic acids, with the assistance of high-throughput experimentation(HTE) and machine learning(ML). A variety of P(O)O-H derivatives, including diarylphosphates, alkyl phosphates, and alkoxyphosphates, are competent candidates to react with sulfoxonium ylides in this transformation, and variousα-phosphoryloxy carbonyls and propylene phosphates are directly constructed. This approach utilizes readily available sulfoxonium ylide as a carbene precursor, and features mild conditions, operational simplicity, and broad functional groups tolerance, and could be used for late-stage functionalization of structurally complex bioactive molecules. Moreover, a conducive exploration of the reaction space is also conducted(756 reactions) and a machine learning model for reaction yield prediction has been developed and applied, showcasing the practical application of this newly workflow(HTE-ML) in the field of synthetic chemistry.

关键词：

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

病毒性疾病基因修饰小鼠模型的研究进展

病毒学报 2024年第3期40卷 609-617页

作者：吴海婷范胜涛张才星李牧遥张艺薇黄璋琼北京协和医学院中国医学科学院医学生物学研究所昆明650118 澳门科技大学中国澳门999078

随着腺相关病毒、慢病毒载体转染及靶向核酸酶等基因修饰技术迅速发展,其被广泛应用于构建病毒性疾病基因修饰小鼠模型,这对于研究病毒的结构、功能及致病机制具有重要的价值。本文就近年来构建的基因修饰小鼠病毒感染模型进行梳理,讨... 详细信息

随着腺相关病毒、慢病毒载体转染及靶向核酸酶等基因修饰技术迅速发展,其被广泛应用于构建病毒性疾病基因修饰小鼠模型,这对于研究病毒的结构、功能及致病机制具有重要的价值。本文就近年来构建的基因修饰小鼠病毒感染模型进行梳理,讨论基因修饰技术在多种病毒性疾病小鼠模型中的研究进展,分析这些模型对在病毒性疾病的预防和治疗中的应用前景,以期为病毒性疾病相关的治疗药物和疫苗研究提供参考。

关键词：基因修饰病毒性疾病小鼠模型

维普期刊数据库

在线全文

学校读者我要写书评

暂无评论

核定位人源α-突触核蛋白转基因小鼠的建立

中国实验动物学报 2024年第3期32卷 307-316页

作者：韦梦晨范胜涛吴海婷张艺薇王子鸥黄璋琼北京协和医学院中国医学科学院医学生物学研究所昆明650118

目的建立一种核定位人源α-突触核蛋白(α-synuclein, α-syn)转基因小鼠,并探究人源α-syn核输入对小鼠行为的影响。方法构建hSNCA-NLS和EGFP的慢病毒载体,利用显微注射法建立转基因小鼠模型,通过PCR和Western Blot方法鉴定转基因首... 详细信息

目的建立一种核定位人源α-突触核蛋白(α-synuclein, α-syn)转基因小鼠,并探究人源α-syn核输入对小鼠行为的影响。方法构建hSNCA-NLS和EGFP的慢病毒载体,利用显微注射法建立转基因小鼠模型,通过PCR和Western Blot方法鉴定转基因首建鼠及其子代基因型和蛋白表达情况。采用免疫荧光鉴定人源α-syn在小鼠脑组织中的共定位情况,同时采用旷场、转棒、O迷宫实验评价转基因小鼠的行为改变情况。结果 hSNCA-NLS基因成功插入小鼠基因组中,人源α-syn成功表达,并且有明显的核定位现象。进一步研究发现,核定位人源α-syn转基因小鼠在2月龄时开始表现出了运动功能障碍,发生了星形胶质细胞增生和炎症反应,于9月龄时表现出了明显的焦虑样症状,γ-氨基丁酸(GABA)基因表达减少,这些表现一直持续到小鼠12月龄。结论成功构建了核定位人源α-syn转基因小鼠,小鼠表现出明显的运动功能障碍和焦虑样症状。该模型的成功建立可以为研究α-syn核定位现象在帕金森病中的作用提供一定的研究基础。

关键词：人源α-突触核蛋白小鼠核定位转基因运动功能障碍

维普期刊数据库

Location of Asymmetric Ground Fault Using Virtual Injected Current Ratio and Two-stage Recovery Strategy in Distribution Networks

在线全文

学校读者我要写书评

暂无评论

CSEE Journal of Power and Energy Systems 2024年第1期10卷 151-161页

作者： haiting Shan Luliang Zhang Q.H.wu Mengshi Li School of Electric Power Engineering South China University of Technology(SCUT)Guangzhou 510641China

Sparse measurements challenge fault location in distribution *** paper proposes a method for asymmetric ground fault location in distribution networks with limited measurements.A virtual injected current vector is formulated to estimate the fault line,which can be reconstructed from voltage sags measured at a few buses using compressive sensing(CS).The relationship between the virtual injected current ratio(VICR)and fault position is deduced from circuit analysis to pinpoint the ***,a two-stage recovery strategy is proposed for improving reconstruction accuracy of the current vector,where two different sensing matrixes are utilized to improve the *** proposed method is validated in IEEE 34 node test *** results show asymmetric ground fault type,resistance,fault position and access of distributed generators(DGs)do not significantly influence performance of our *** addition,it works effectively under various scenarios of noisy measurement and line parameter *** on 134 node test feeders prove the proposed method is also suitable for systems with more complex structure.

关键词： Distribution networks fault location reconstruction accuracy two-stage recovery virtual injected current ratio

Analysis of China's Punitive Damages System

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of China's Punitive Damages System

Anaerobic high-density in vitro culture of Akkermansia muciniphila in a simple medium combined with pseudo-exponential glucose feeding and pH-controlling strategy

作者： haiting wu China Jiliang University

There have been continuous disputes over the nature of the punitive damages system since China's first reference in the "Consumer Rights Protection Act". The theory of public law responsibility, the private law responsibility and public and private law responsibility, each of these three doctrines has its own words. The author believes that since the punitive compensation provisions are in civil legislation, it should be considered a private law responsibility. The function of punitive damages also has a view from dualism to pluralism. The author believes that the system only has the function of punishment and compensation, and other functions can be considered as the derivative functions of the two basic functions. Secondly, regarding the determination of the amount of punitive damages, the author compares the four methods of determination in China and gives some factors that he thinks should be considered. Finally, regarding the existence and punishment of punitive damages, the author believes that the punitive damages system is beneficial to China's legal system. The punitive damages system should continue to play its due role in the construction of China's legal system and China should constantly improve the mechanism to make it more compatible with the reality of China.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Anaerobic high-density in vitro culture of Akkermansia mucin...

Evaluation of the Preventive Effect of Regional Cooling Nursing on Hand Foot Syndrome Caused by Doxorubicin Hydrochloride Liposome

第五届益生菌益生元科学大会

作者： haiting wu Yinghua Lu Song Huang Xueping Ling Department of Chemical and Biochemical Engineering College of Chemistry and Chemical EngineeringXiamen University Bluepha Co. Ltd

Akkermansia muciniphila（***） is being considered as a potential next-generation probiotic due to its impressive physiological functions such as enhancing insulin sensitivity,promoting weight loss and improving intestinal barrier ***-density cultivation of *** in vitro at a low cost is a promising and urgent research ***,an optimized medium with simple ingredients was attained through eliminating the nonessential inorganic salts,vitamins,and trace metal ions,which achieved high OD value of 5.2 in the shake *** large-scale culture,a strategy of pseudo-exponential glucose feeding and stage pHcontrolling was investigated in a 5-L bioreactor,which led to a significant improvement in the cell density of ***,reaching OD value of 22.03(1.18×10 CFU/mL),which was 3.13 times higher than the highest reported value(OD = 7.02).Taken together,the present work provides a more conducive fermentation strategy for the industrial production of *** in vitro with a reduced production cost.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Open Journal of Nursing 2022年第11期12卷 772-781页

作者： Yue Li Wanwei Huang Lijuan Zhang Lijun Jiang Xiaohong Lin haiting wu Yuting Huang Na Li Department of Nursing Sun Yat-Sen University Cancer Center Guangzhou China

Purpose: To explore the preventive effect of Regional cooling comprehensive nursing on hand foot syndrome caused by pegylated liposomal doxorubicin (PLD). Method: Adopt overall sampling method. Patients who used the same adjuvant drugs from January to December 2020 were randomly divided into an intervention group and a control group. The patients in the two groups received routine nursing guidance and drug prevention for the use of amygdalin. The patients in the intervention group were required to take protective measures of Regional cooling during chemotherapy. The occurrence of hand foot syndrome during adriamycin liposome administration was compared between the two groups. Results: By comparing the adverse reactions of cases during Adriamycin Administration, the incidence rates of grade I, II and III hand foot syndrome in the control group were 28.8%, 7.6% and 27.5% respectively, and the incidence rates of grade I, II and III hand foot syndrome in the intervention group were 42.1%, 12.3% and 7.0% respectively, with statistical significance (P Conclusion: Regional cooling nursing and preventive behavior guidance can effectively reduce the severity of hand foot syndrome caused by adriamycin.

关键词： Hand-Foot Syndrome Regional Cooling Pegylated Liposomal Doxorubicin

超小剂量利妥昔单抗治疗ANCA相关性血管炎一例

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

协和医学杂志 2023年第6期14卷 1330-1334页

作者：吴海婷李航中国医学科学院北京协和医院肾内科北京100730

本文报道一例肺、肾受累的抗中性粒细胞胞浆抗体(antineutrophil cytoplasmic antibodies, ANCA)相关性血管炎患者,给予经典的糖皮质激素、环磷酰胺、血浆置换诱导缓解治疗有效。该患者在使用硫唑嘌呤维持治疗期间共出现2次感染,后换用... 详细信息

本文报道一例肺、肾受累的抗中性粒细胞胞浆抗体(antineutrophil cytoplasmic antibodies, ANCA)相关性血管炎患者,给予经典的糖皮质激素、环磷酰胺、血浆置换诱导缓解治疗有效。该患者在使用硫唑嘌呤维持治疗期间共出现2次感染,后换用超小剂量(100 mg/次)利妥昔单抗维持治疗病情稳定。个体化利妥昔单抗治疗ANCA相关性血管炎效果显著,同时避免了过度免疫抑制,减轻了患者的经济负担。本例患者的诊疗经验有助于提高临床医生在ANCA相关性血管炎维持治疗方面的认识。

关键词：抗中性粒细胞胞浆抗体相关性血管炎利妥昔单抗硫唑嘌呤

维普期刊数据库

A Study on the Effect of Comprehensive Intervention Based on Transition Theory on the Readiness for Discharge of Post-Operative Breast Cancer Patients

在线全文

学校读者我要写书评

暂无评论

Advances in Breast Cancer Research 2022年第2期11卷 128-140页

作者： Na Li Wanwei Huang Lijun Jiang Lijun Zhang Xiaohong Lin haiting wu Yuting Huang Yue Li Department of Nursing Sun Yat-sen University Cancer Center Guangzhou China

Purpose: To explore the effect of comprehensive intervention based on transition theory in enhancing the readiness for discharge of post-operative breast cancer patients. Method: In a non-simultaneous controlled study test, 99 post-operative breast cancer patients hospitalized between August 2019 and February 2020 were selected as the control group and 93 post-operative breast cancer patients hospitalized between March and August 2020 were selected as the test group at a Grade A tertiary tumor hospital. While the control group used the conventional care model, the test group added a comprehensive intervention based on transition theory to the routine nursing, which focused on stimulating patients’ awareness of health transitions, assisting patients to identify health critical events, strengthening social support and promoting self-management. The differences between the two groups were compared in terms of patient readiness for discharge and patient evaluation of the quality of discharge teaching. Result: The total score of readiness for discharge was higher in the test group than in the control group (171.7 ± 24.5 vs. 155.9 ± 28.9) and the scores for “Self-condition” (55.6 ± 8.2 vs. 50.8 ± 9.7), “Disease Knowledge” (64.7 ± 13.1 vs. 57.7 ± 13.8), “Coping Ability after Discharge” (24.6 ± 4.8 vs. 22.2 ± 5.6), “Expected Social Support after Discharge” (26.8 ± 4.5 vs. 25.2 ± 4.8) were higher than those of the control group, and all differences were statistically significant (p < 0.05). The total score of the test group on the quality of discharge teaching scale was higher than that of the control group (162.1 ± 19.9 vs. 152.6 ± 28.7), and the scores for “Content that Patients Self-perceived they required before discharge” (53.2 ± 9.5 vs. 49.4 ± 12.1), “Content that Patients Actually Acquired before Discharge” (51.2 ± 9.2 vs. 48.3 ± 11.3) and “Guiding Skills and Effects of Discharge for Nurses” (110.9 ± 12.6 vs. 104.3 ± 19.00) were also higher than those of the control g

关键词： Transition Theory Breast Cancer Readiness for Discharge

Factors Influencing Readiness for Hospital Discharge among Patients Undergoing Breast Cancer Surgery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2022年第7期13卷 486-496页

作者： Na Li Wanwei Huang Lijuan Zhang Lijun Jiang Xiaohong Lin haiting wu Yuting Huang Yue Li Department of Nursing Sun Yat-sen University Cancer Center Guangzhou China

Background: Breast cancer has become an important disease burden harmful to women’s health. The treatment of breast cancer is mainly surgical treatment. In the context of enhanced recovery after surgery, the average length of hospital stay has been reduced, so there is increasing concern about postoperative patient discharge readiness. Objective: To identify factors influencing readiness for hospital discharge among patients having undergone breast cancer surgery and to explore the relationship between discharge readiness and the quality of discharge teaching. Methods: A total of 99 patients after breast cancer surgery at a regional tertiary medical tumor center in Guangzhou between June 2021 and December 2021 were collected using the convenience sampling method. A general data questionnaire, readiness for hospital discharge scale, and quality of discharge teaching scale were used to conduct a questionnaire survey on the subjects. Results: 83.8% of the patients feel ready for discharge, and the score of discharge readiness was 155.98 ± 28.87. The patients’ content received and the skills in teaching delivery were the influencing factors of discharge readiness. Discharged with a drainage tube was an independent factor of discharge readiness. Conclusions: Readiness for hospital discharge among patients undergoing breast cancer surgery was above medium level, and perfect intervention measures should be formulated for the influencing factors of discharge readiness. Especially, specific executable safeguard measures should be formulated for patients who are discharged early after surgery to promote the safety of home care.

关键词： Breast Cancer Discharge Readiness Factors Discharge Teaching Quality