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Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode

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Science China Chemistry 2009年第8期52卷 1128-1135页

作者： hailing chen Li Zhao Xi chen ZhiXia Zhuang XiaoRu Wang Testing Center for Food and Drug Safety Huaxia Vocational College Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate (ormosil)-polyvinyl acetate (PVA) matrix onto a Prussian Blue (PB)-modified glassy carbon electrode. A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3, K3[Fe(CN)6] and ethylenediamine tetraacetic acid (EDTA) under cyclic voltammetric (CV) conditions. The effects of the potential range of CV conditions, electrolyte cations, applied potential, pH, temperature and co-existing substances were investigated. The detection limit of the glucose biosensor was 8.1 μmol·L−1 (S/N = 3) with a linear range from 20 μmol·L−1 to 2 mmol·L−1 (R = 0.9965). The biosensor presented a fast response and good selectivity. Additionally, excellent reproducibility and stability of the biosensor were observed.

关键词： Prussian Blue glucose oxidase ormosil-PVA ethylenediamine tetraacetic acid biosensor

Specification Requirement for Thermal Stability of Sintered NdFeB Materials for Electrical Machines

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Journal of Rare Earths 2004年第z1期22卷 93-95页

作者： Lin Yan Jiang Daiwei chen Lixiang chen hailing Bi Haitao Tang Renyuan Research Institute of special Electrical Machine Shenyang University of Technology Shenyang 110023 China

Based on IEC standards and Chinese national standards of sintered NdFeB materials, in the paper the hightemperature, room-temperature properties and thermal stability of about one hundred samples of NdFeB materials for electrical machines were measured and *** materials are produced by ten representative manufactories in *** with the analysis results, the paper points out that the magnetic properties of sintered NdFeB materials for electrical machines should meet not only the specific values in standards, such as Br, (BH)max ,HcJ ,but also the requirement of temperature coefficients a (Br) , a (HcJ).

关键词： NdFeB temperature coefficient thermal stability

Symbolic algorithmic verification of intransitive generalized noninterference

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Science China(Information Sciences) 2012年第7期55卷 1650-1665页

作者： ZHOU CongHua,LIU ZhiFeng,WU hailing,chen Song & JU ShiGuang School of Computer Science and Telecommunication Engineering,Jiangsu University Zhenjiang 212013,China 1. School of Computer Science and Telecommunication Engineering Jiangsu University Zhenjiang 212013 China

Generalized noninterference can be used to formulate transitive security policies,but is unsuitable for intransitive security *** propose a new information flow security property,which we call intransitive generalized noninterference,that enables intransitive security policies to be specified ***,we propose an algorithmic verification technique to check intransitive generalized *** technique is based on the search for counterexamples and on the window induction proof,and can be used to verify generalized *** further demonstrate that the search of counterexamples and induction proof can be reduced to quantified Boolean *** reduction enables us to use efficient quantified Boolean decision procedures to perform the check of intransitive generalized *** also reduces spatial requirement by representing the space compactly,and improves the efficiency of the verification procedure.

关键词： intransitive generalized noninterference quantified Boolean satisfiability symbolic verification multi-level security

A bifunctional vinyl-sulfonium tethered peptide induced by thio-Michael-type addition reaction

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Chinese Chemical Letters 2022年第4期33卷 2001-2004页

作者： Hongkun Xu Xuan Qin Yaping Zhang Chuan Wan Rui Wang Zhanfeng Hou Xiaofeng Ding hailing chen Ziyuan Zhou Yang Li chenshan Lian Feng Yin Zigang Li State Key Laboratory of Chemical Oncogenomics School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhen 518055China Pingshan translational medicine center Shenzhen Bay LaboratoryShenzhen 518055China Anhui Medical University Hefei 230032China Cancer Hospital Chinese Academy of Medical Sciences Shenzhen CenterShenzhen 518000China

The modification and functionalization of peptides is of great significance in modern biotechnology and drug development. Here we report a highly reactive Michael-type warhead for the covalently modification of cysteine on peptide and protein. By installing a vinyl group onto a methionine residue of peptide,the produced vinyl sulfonium can be efficiently nucleophilic added by appropriate cysteine residue of this peptide, and thus yield a cyclized peptide. This peptide cyclization strategy was proven to exhibit improved cell penetration and good stability. Moreover, a peptide ligand bearing vinyl sulfonium could covalently bind to the cysteine in the target protein, indicating the potential of vinyl sulfonium as a novel warhead for developing covalent peptide inhibitor.

关键词： Vinyl sulfonium Michael-type addition Peptide cyclization Covalent peptide inhibitor Proximity-induced ligation

Numerical Simulation of Transient 3-D Turbulent Hested Jet into Crossflow in a Thick-Wall T-Junction Pipe

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Journal of Thermal Science 2001年第1期10卷 46-51页

作者： Wu hailing, chen Tingkuan, Luo Yushan, Wang Haijun (State Key Laboratory of Multiphase Flow in Power Engineering,Xi’an Jiaotong University, Xi’an 710049, China) State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong University Xi’an China

The present work is to investigate the transient three-dimensional heated turbulent jet into crossflow in a thickwall T-junction pipe using CFD package. Two cases with the jet-to-crossflow velocity ratio of 0.05 and 0.5 are computed, with a finite-volume method utilizing κ-ε model. Comparison of the steady-state computations with measured data shows good qualitative agreement. Transient process of injection is simulated to examine the thermal shock on the T-junction component. Temporal temperature of the component is acquired by thermal coupling with the fluid. Via analysis of the flow and thermal characteristics, factors causing the thermal shock are studied. Optimal flow rates are discussed to reduce the thermal shock.

关键词： numerical simulation low jet-to-crossflow velocity ratio thick-wall T-junction pipe thermal shock

“产品—社会”服务系统创新:基于GiveMeTap项目的可持续设计思考

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装饰 2022年第1期 36-41页

作者：丁熊陈海玲广州美术学院工业设计学院

以GiveMeTap项目为研究对象,梳理其创业动机、品牌发展历程、运营模式及取得的商业成果。运用产品服务系统、社会创新理论及可持续设计评价标准对案例展开深入剖析,从而提出产品服务系统介入社会创新的新方向,即“产品—社会”服务系统... 详细信息

以GiveMeTap项目为研究对象,梳理其创业动机、品牌发展历程、运营模式及取得的商业成果。运用产品服务系统、社会创新理论及可持续设计评价标准对案例展开深入剖析,从而提出产品服务系统介入社会创新的新方向,即“产品—社会”服务系统。该系统为不同利益相关者提出了解决复杂社会问题的新思路和新范式,其特征体现为:为特定问题而创新;以社会利益为前提;参与式的协同合作;搭建弹性生态系统。

关键词：可持续设计产品服务系统社会创新 GiveMeTap “产品—社会”服务系统

硫化锰夹杂溶解和微生物存在对不锈钢管的点蚀

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应用化学 2012年第1期29卷 100-105页

作者：姬鄂豫陈海玲李长鸣南阳理工学院南阳473004 河南电力试验研究院郑州450007

结合发电厂凝汽器不锈钢管内壁腐蚀部位分布规律和腐蚀外貌特征,分别对腐蚀部位沉积物、内壁蚀孔附近管材成分进行扫描电子显微镜观察和能谱分析,探讨了管材中MnS溶解与微生物对凝汽器不锈钢管腐蚀的电化学反应机理,发现二者在点蚀形成... 详细信息

结合发电厂凝汽器不锈钢管内壁腐蚀部位分布规律和腐蚀外貌特征,分别对腐蚀部位沉积物、内壁蚀孔附近管材成分进行扫描电子显微镜观察和能谱分析,探讨了管材中MnS溶解与微生物对凝汽器不锈钢管腐蚀的电化学反应机理,发现二者在点蚀形成过程中具有协同作用,并在综合分析影响不锈钢腐蚀的各种因素的基础上,提出了不锈钢凝汽器在运行过程中应采取的防护措施。

关键词：凝汽器不锈钢管 MnS溶解微生物腐蚀

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轴向和螺旋磁场中的自由电子相干辐射

光学学报 1984年第9期4卷 791-796页

作者：王海林陈建文中国科学院上海光学精密机械研究所

本文用单粒子理论方法,详细讨论了螺旋磁场近磁共振下的电子相空间运动方程和辐射场方程,得到了近磁共振下电子辐射的频率红移,谱线展宽和增益增强.我们的理论还适用于变周期和梯度螺旋磁场的情况.

关键词：螺旋磁场电子磁共振轻子轴向方程

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敦煌莫高窟第465窟温湿度时空分布特征

干旱区资源与环境 2018年第2期32卷 155-160页

作者：陈海玲陈港泉薛平侯文芳敦煌研究院保护研究所敦煌736200 国家古代壁画与土遗址保护工程技术研究中心敦煌736200 甘肃省古代壁画与土遗址保护重点实验室敦煌736200

通过对莫高窟北区第465窟温湿度进行连续监测,研究其时空分布特征。结果表明:第465窟温度大致呈正弦半波曲线变化,8月温度最高,1月温度最低,相对湿度受降雨影响明显,最高相对湿度出现在降雨量最大的8月。洞窟内温湿度空间分布受围护结... 详细信息

通过对莫高窟北区第465窟温湿度进行连续监测,研究其时空分布特征。结果表明:第465窟温度大致呈正弦半波曲线变化,8月温度最高,1月温度最低,相对湿度受降雨影响明显,最高相对湿度出现在降雨量最大的8月。洞窟内温湿度空间分布受围护结构热惯性影响,表现出衰减性和延迟性,夏季由窟门向内温度逐渐降低,相对湿度逐渐升高,冬季相反。主室温湿度在空间分布上较前室更为均匀,垂直方向温湿度分布与高度相关性较大,距地面1.8m以上空间温湿度分布极为均匀。热压以及湿度差驱动的洞窟内外热湿交换造成了温湿度波动,夏季窟内中上部温湿度日波动较大,冬季前室地面温湿度日波动较大。

关键词：莫高窟温湿度监测时空分布