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Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury

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Neural Regeneration Research 2024年第8期19卷 1822-1827页

作者： Yuhui Kou Yusong Yuan Qicheng Li Wenyong Xie hailin xu Na Han Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina National Center for Trauma Medicine BeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina Department of Orthopedics China-Japan Friendship HospitalBeijingChina Department of Central Laboratory and Institute of Clinical Molecular Biology Peking University People’s HospitalBeijingChina

Macrophages play an important role in peripheral nerve regeneration,but the specific mechanism of regeneration is still *** preliminary findings indicated that neutrophil peptide 1 is an innate immune peptide closely involved in peripheral nerve ***,the mechanism by which neutrophil peptide 1 enhances nerve regeneration remains *** study was designed to investigate the relationship between neutrophil peptide 1 and macrophages in vivo and in vitro in peripheral nerve crush *** functions of RAW 264.7 cells we re elucidated by Cell Counting Kit-8 assay,flow cytometry,migration assays,phagocytosis assays,immunohistochemistry and enzyme-linked immunosorbent *** debris phagocytosis was observed using the CUBIC(Clear,Unobstructed Brain/Body Imaging Cocktails and Computational analysis)optical clearing technique during Wallerian *** inflammatory factor expression in different polarization states was detected using a protein *** results showed that neutrophil peptide 1 promoted the prolife ration,migration and phagocytosis of macrophages,and CD206 expression on the surfa ce of macrophages,indicating M2 *** axonal debris clearance rate during Wallerian degeneration was enhanced after neutrophil peptide 1 *** peptide 1 also downregulated inflammatory factors interleukin-1α,-6,-12,and tumor necrosis factor-αin invo and in ***,the results suggest that neutrophil peptide 1 activates macrophages and accelerates Wallerian degeneration,which may be one mechanism by which neutrophil peptide 1 enhances peripheral nerve regeneration.

关键词： axonal debris inflammatory factors macrophages neutrophil peptide 1 peripheral nerve injury peripheral nerve regeneration RAW 264.7 cells sciatic nerve Wallerian degeneration

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S-Nitrosylation-mediated coupling of DJ-1 with PTEN induces PI3K/AKT/mTOR pathway-dependent keloid formation

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Burns & Trauma 2023年第1期11卷 536-549页

作者： Dongming Lv Zhongye xu Pu Cheng Zhicheng Hu Yunxian Dong Yanchao Rong hailin xu Zhiyong Wang Xiaoling Cao Wuguo Deng Bing Tang Department of Burn and Plastic Surgery the First Affiliated Hospital of Sun Yat-sen University58 Zhongshan Road IIGuangzhou 510080China Department of General Surgery the Seventh Affiliated Hospital of Sun Yat-sen University628 Zhenyuan RoadShenzhenChina Department of Plastic Surgery Guangdong Second Provincial General HospitalSouthern Medical University466 Xingang Middle RoadGuangzhouChina Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer Medicine651 Dongfeng East RoadGuangzhouChina

Background:Keloids are aberrant dermal wound healing characterized by invasive growth,extracellular matrix deposition,cytokine overexpression and easy *** factors have been implicated as pathological causes of keloids,particularly hyperactive inflammation,tension alignment and genetic predisposition.S-Nitrosylation(SNO),a unique form of protein modification,is associated with the local inflammatory response but its function in excessive fibrosis and keloid formation remains *** aimed to discover the association between protein SNO and keloid ***:Normal and keloid fibroblasts were isolated from collected normal skin and keloid *** obtained fibroblasts were cultured in DMEM supplemented with 10%fetal bovine serum and 1%penicillin/*** effects of DJ-1 on cell proliferation,apoptosis,migration and invasion,and on the expression of proteins were ***-based proximity labelling and liquid chromatography-mass spectrometry were conducted to explore the potential targets of ***-switch assays and transnitrosylation reactions were used to detect protein *** data were compared by two-tailed Student’s t ***:We found that DJ-1 served as an essential positive modulator to facilitate keloid cell proliferation,migration and invasion.A higher S-nitrosylated DJ-1(SNO-DJ-1)level was observed in keloids,and the effect of DJ-1 on keloids was dependent on SNO of the Cys106 residue of the DJ-1 ***-DJ-1 was found to increase the level of phosphatase and tensin homolog(PTEN)S-nitrosylated at its Cys136 residue via transnitrosylation in keloids,thus diminishing the phosphatase activity of PTEN and activating the PI3K/AKT/mTOR ***,Cys106-mutant DJ-1 is refractory to SNO and abrogates DJ-1-PTEN coupling and the SNO of the PTEN protein,thus repressing the PI3K/AKT/mTOR pathway and alleviating keloid ***,the biological effect of DJ-1 in keloids is dependent on the

关键词： DJ-1 PTEN Protein S-nitrosylation Transnitrosylation Keloid S-Nitrosylation

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Lung Single-Cell Transcriptomics Offers Insights into the Pulmonary Interstitial Toxicity Caused by Silica Nanoparticles

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Environment & Health 2024年第11期2卷 786-801页

作者： Yan Li Qing Yao hailin xu Jiaze Ren Yurou Zhu Caixia Guo Yanbo Li Department of Occupational Health and Environmental Health School of Public Health and Beijing Key Laboratory of Environmental ToxicologyCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology Capital Medical UniversityBeijing 100069China Department of Toxicology and Sanitary Chemistry School of Public HealthCapital Medical UniversityBeijing 100069China

The adverse respiratory outcomes motivated by silica nanoparticles(SiNPs)exposure have received increasing ***,we aim to elucidate the interplay of diverse cell populations in the lungs and key contributors in triggering lung injuries caused by *** conducted a subchronic respiratory exposure model of SiNPs via intratracheal instillation in Wistar rats,where rats were administered with 1.5,3.0,or 6.0 mg/kg body weight SiNPs once a week for 12 times in *** revealed that SiNPs caused pulmonary interstitial injury in rats by histopatho-logical examination and pulmonary hydroxyproline ***,a single-cell RNA-Seq via screening 10457 cells in the rat lungs disclosed cell-specific responses to SiNPs and cell-to-cell interactions within the alveolar macrophages,epithelial cells,and fibroblasts from rat *** disturbed responses were principally related to the dysregulation of protein homeostasis(proteostasis),accompanied by an inflammatory response in macrophages,cell death in epithelial,proliferation,and extracellular matrix deposition in *** cell-specific responses may serve a synergistic role in the pathogenesis of pulmonary interstitial disease triggered by *** particular,the analyses of gene interaction networks and gene−disease associations filtered out heat shock proteins(Hsps)family genes crucial to the observed pulmonary lesions caused by *** note,both GEO database analysis and our experiments’validation indicated that Hsps,especially Hspd1,may be a key contributor to pulmonary interstitial injury,possibly through triggering oxidative stress,immune response,and disrupting protein *** together,our study provides insights into pulmonary toxic effects and underlying molecular mechanisms of SiNPs from a single-cell perspective.

关键词： silica nanoparticles single-cell RNA-Seq lung toxicity lung interstitial disease proteostasis

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Ultrasensitive biosensors based on long-range surface plasmon polariton and dielectric waveguide modes

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Photonics Research 2016年第6期4卷 262-266页

作者： Leiming Wu Jun Guo hailin xu Xiaoyu Dai Yuanjiang Xiang SZU-NUS Collaborative Innovation Center for Optoelectronic Science & Technology Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen University College of Physics and Energy Shenzhen University

An ultrasensitive biosensor based on hybrid structure and composed of long-range surface plasmon polariton(LRSPP) and dielectric planar waveguide(PWG) modes is proposed. Both PWG and LRSPP modes have strong resonances to form strong coupling between the two modes, and the two modes can couple to enhance sensitivity of sensors. In the hybrid structure, PWG is composed of cytop–Si–cytop multilayers and the LRSPP configuration is composed of cytop–metal–sensing medium multilayer slabs. The highest imaging sensitivities of 2264 and3619 RIU-1were realized in the proposed sensors based on Au and Al-monolayer graphene, respectively, which are nearly 1.2 and 1.9 times larger than the 1910 RIU-1sensitivity of the conventional LRSPR sensor(LRSPP sensor). Moreover, it is demonstrated that the PWG-coupled LRSPP biosensor is applicable to the sensing medium,with refractive index in the vicinity of 1.34.

关键词： RIU Ultrasensitive biosensors based on long-range surface plasmon polariton and dielectric waveguide modes

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Meta-Analysis of Ventilated versus Spontaneously Breathing Patients in Predicting Fluid Responsiveness by Inferior Vena Cava Variation

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International Journal of Clinical Medicine 2018年第10期9卷 760-777页

作者： Xiang Si Daiyin Cao hailin xu Xiangdong Guan Department of Surgical Intensive Care Unit The First Affiliated Hospital of Sun Yat-sen University Guangzhou China Department of Critical Care Medicine The Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou China Transplantation Department The Second Affiliated Hospital of Guangzhou Medical University Guangzhou China

Purpose: Respiratory variation in inferior vena cava (ΔIVC) has been extensively studied in predicting fluid responsiveness, but the results are conflicting. We performed a systemic review and meta-analysis of studies aiming at investigating the diagnostic accuracy of ΔIVC in predicting fluid responsiveness. Methods: MEDLINE, EMBASE, Cochrane Database and Web of Science were screened for relevant original and review articles from inception to July 2016. The meta-analysis determined the pooled sensitivity, specificity, diagnostic odds ratio (DOR) and area under the ROC curve (AUROC). In addition, subgroup analyses were performed in mechanically ventilated patients and spontaneously breathing patients. Results: A total of 20 studies involving 635 patients were included. Cutoff values of ΔIVC varied from 12% to 42%, the pooled sensitivity and specificity was 0.68 (0.62 - 0.75) and 0.80 (0.75 - 0.85), respectively. The DOR was 14.2 (6.0 - 33.6) and the AUROC was 0.86 (0.78 - 0.93). Subgroup analysis showed better diagnostic performance in patients on mechanical ventilation than in spontaneously breathing patients with higher sensitivity (0.75 vs. 0.56), specificity (0.82 vs. 0.78), DOR (22.9 vs. 7.9) and AUROC (0.90 vs. 0.80). The best threshold of ΔIVC in patients on mechanical ventilation was IVC distensibility index (ΔIVC ≥17% ±4%), compared to IVC collapsibility index (ΔcIVC ≥33% ±12%) in spontaneously breathing patients. Conclusion: ΔIVC is not an accurate predictor of fluid responsiveness in patients with acute circulatory failure. In patients on mechanical ventilation, the predicting ability of ΔIVC was moderate with acceptable sensitivity and specificity;in spontaneously breathing patients, the specificity remains acceptable but its sensitivity is poor.

关键词： Fluid Responsiveness Inferior Vena Cava Variation

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黑磷/介质多层结构包覆的可调谐波导

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激光与光电子学进展 2021年第23期58卷 270-276页

作者：徐海林深圳大学物理与光电工程学院广东深圳518061

从理论上提出了一种由黑磷/介质多层结构包覆的等离子体波导。利用MATLAB数值求解该波导的色散方程,进而仿真出波导的有效折射率、传播长度、趋肤深度及品质因数等性能参数曲线。由仿真曲线可知,波导的性能与黑磷/介质多层结构中的电子... 详细信息

从理论上提出了一种由黑磷/介质多层结构包覆的等离子体波导。利用MATLAB数值求解该波导的色散方程,进而仿真出波导的有效折射率、传播长度、趋肤深度及品质因数等性能参数曲线。由仿真曲线可知,波导的性能与黑磷/介质多层结构中的电子掺杂率正相关,与单元层中介质层厚度负相关。与同类型金属/介质多层结构波导的性能比较后可知,黑磷/介质多层结构波导在中红外及远红外波段具更大的传播长度、更小的趋肤深度及更大的品质因数。该波导可调谐且性能优良。

关键词：材料波导黑磷/介质多层结构可调谐性电子掺杂率有效介电常数

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Melatonin influences the biological characteristics of keloid fibroblasts through the Erk and Smad signalling pathways

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Burns & Trauma 2023年第1期11卷 258-272页

作者： Shaobin Huang Wuguo Deng Yunxian Dong Zhicheng Hu Yi Zhang Peng Wang Xiaoling Cao Miao Chen Pu Cheng hailin xu Wenkai Zhu Bing Tang Jiayuan Zhu Department of Burn First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Department of Cosmetic and Plastic Surgery The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhou 510060China Department of Burn and Plastic Surgery Affiliated Hospital of Nantong UniversityNantong 226001China Department of Obstetrics and Gynecology School of MedicineStanford UniversityStanfordCA 94305United States

Background:Keloids are abnormal fibrous hyperplasias that are difficult to *** can be used to inhibit the development of certain fibrotic diseases but has never been used to treat *** aimed to discover the effects and mechanisms of melatonin in keloid fibroblasts(KFs).Methods:Flow cytometry,CCK-8 assays,western blotting,wound-healing assays,transwell assays,collagen gel contraction assays and immunofluorescence assays were applied to demonstrate the effects and mechanisms of melatonin in fibroblasts derived from normal skin,hypertrophic scars and *** therapeutic potential of the combination of melatonin and 5-fluorouracil(5-FU)was investigated in ***:Melatonin significantly promoted cell apoptosis and inhibited cell proliferation,migration and invasion,contractile capability and collagen production in *** mechanistic studies demonstrated that melatonin could inhibit the cAMP/PKA/Erk and Smad pathways through the membrane receptor MT2 to alter the biological characteristics of ***,the combination of melatonin and 5-FU remarkably promoted cell apoptosis and inhibited cell migration and invasion,contractile capability and collagen production in ***,5-FU suppressed the phosphorylation of Akt,mTOR,Smad3 and Erk,and melatonin in combination with 5-FU markedly suppressed the activation of the Akt,Erk and Smad ***:Collectively,melatonin may inhibit the Erk and Smad pathways through the mem-brane receptor MT2 to alter the cell functions of KFs,while combination with 5-FU could exert even more inhibitory effects in KFs through simultaneous suppression of multiple signalling pathways.

关键词： Keloid Fibroblast,Melatonin,5-fluorouracil,Erk,Smad Signalling pathways Hyperplasias Phosphorylation

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Metallic resurfacing hemiarthroplasty of the first metatarsophalangeal joint combined with first metatarsal osteotomy for the treatment of hallux rigidus with hallux valgus in China

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Chinese Medical Journal 2014年第11期127卷 2186-2188页

作者： Jin Kaiji Wang Yuanli Fu Zhongguo An Shuai xu hailin Jiang Baoguo Department of Orthopedics & Trauma Peking University People's Hospital Beijing 100044 China Department of Orthopedics Peking Longfu Hospital Beijing 100010 China

Hallux rigidus occurs mainly at the first metatarsophalangeal （MTP） joint,causing localized pain and degenerative arthritis.1 This condition frequently occurs together with hallux valgus and is difficult to *** hallux rigidus affecting only one joint,the treatment choices include articular debridement,resection arthroplasty,prosthetic arthroplasty,and arthrodesis,depending on the degree of arthritis.

关键词： hallux rigidus hallux valgus metallic resurfacing hemiarthroplasty metatarsophalangeal joint

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用户互动负荷模型及其微电网日前经济调度的应用

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电网技术 2016年第7期40卷 2009-2015页

作者：孙川汪隆君许海林华南理工大学电力学院广东省广州市510640

建立了可平移负荷、可削减负荷和可转移负荷3类互动负荷模型,应用于用户参与互动环境下微电网日前经济调度,并探讨了用户互动合约的相关内容。可平移负荷模型通过0-1变量和功率分布向量描述,极大地降低了模型求解规模;可削减负荷模型考... 详细信息

建立了可平移负荷、可削减负荷和可转移负荷3类互动负荷模型,应用于用户参与互动环境下微电网日前经济调度,并探讨了用户互动合约的相关内容。可平移负荷模型通过0-1变量和功率分布向量描述,极大地降低了模型求解规模;可削减负荷模型考虑了互动频率和持续时间;可转移负荷模型考虑了可转移时间区间;互动负荷模型均保持线性,求解不依赖于初值。算例验证结果表明:1)所提出的模型充分考虑了互动负荷的用电特点和用户要求,工程实操性强;2)可削减负荷能发挥削峰作用,可平移负荷和可转移负荷实现移峰;3)3类互动负荷相互配合,不仅能合理调整负荷曲线,还能有效消纳新能源出力,以实现系统经济运行。

关键词：用户互动互动负荷模型可平移负荷可削减负荷可转移负荷微电网日前调度计划

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计及分布式电源功率特性的微电网经济调度模型

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电力系统自动化 2016年第11期40卷 31-38页

作者：汪隆君许海林王钢华南理工大学电力学院广东省广州市510640

针对微电网能量管理所获分布式电源(DG)运行点常常不满足DG功率特性的局限,提出了一种计及DG功率特性的微电网经济调度模型。该模型基于解析几何线性化DG的PQ功率极限,与微电网经济调度模型集成并求解,有效计及了逆变型DG、双馈感应发... 详细信息

针对微电网能量管理所获分布式电源(DG)运行点常常不满足DG功率特性的局限,提出了一种计及DG功率特性的微电网经济调度模型。该模型基于解析几何线性化DG的PQ功率极限,与微电网经济调度模型集成并求解,有效计及了逆变型DG、双馈感应发电机、同步发电机的功率特性。在多时间尺度微电网经济调度框架下,对某微电网系统进行算例分析,验证了所提模型的可行性以及有效性,并在此基础上分析了不同的分段数对DG运行点可行域覆盖率的影响。

关键词：微电网(微网) 经济调度模型混合整数规划分布式电源功率特性

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