检索结果-南通市图书馆

同方期刊数据库

A comprehensive census of lake microbial diversity on a global scale

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第10期62卷 1320-1331页

作者： Jian Yang Hongchen Jiang hailiang dong Yongqin Liu State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430074China State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China Department of Geology and Environmental Earth Science Miami UniversityOxfordOhio 45056USA Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China

Despite recent interest in microbial diversity and community structure of lakes across various spatial scales, a global biogeographic distribution pattern and its controlling factors have not been fully disclosed. Here, we compiled and analyzed 88,334,735 environmental 16S rRNA sequences from 431 lakes across a wide range of geographical distance and environmental conditions(in particular, salinity, 0–373.3 gL^–1). Our results showed that lake sediments inhabit significantly(ANOVA: P<0.001) more diverse microbial communities than lake waters. Non-metric dimensional scaling(NMDS) ordinations indicated that microbial community compositions differed distinctly among sample types(freshwater vs. saline, water vs. sediment) and geographic locations. Mantel and partial Mantel tests showed that microbial community composition in lake water was significantly(P=0.001) correlated with geographic distance, salinity, and pH. Statistical analyses based on neutral community and null models indicated that stochastic processes may play predominant roles in shaping the microbial biogeographic distribution patterns in the studied global lake waters. The dispersal-related stochasticity(e.g., homogenizing dispersal) exhibited a stronger influence on the distribution of microbial community in freshwater lakes than in saline lakes. Overall, this work expands our understanding of the impact of geographic distance, environmental conditions, and stochastic processes on microbial distribution in global lakes.

关键词： microbial biogeography lakes geographic distance environmental factors stochastic processes

Iron and lead ion adsorption by microbial flocculants in synthetic wastewater and their related carbonate formation

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2013年第12期25卷 2422-2428页

作者： Minjie Yao Bin Lian hailiang dong Jianchao Hao Congqiang Liu State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of Sciences Key Laboratory of Environmental and Applied Microbiology Chengdu Institute of BiologyChinese Academy of Sciences Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for MicrobiologyCollege of Life ScienceNanjing Normal University State Key Laboratory of Geobiology and Environmental Geology China University of Geosciences Department of Geology and Environmental Earth Science Miami University

Although microbial treatments of heavy metal ions in wastewater have been studied, the removal of these metals through incorporation into carbonate minerals has rarely been reported. To investigate the removal of Fe^3＋ and Pb^2＋, two representative metals in wastewater, through the precipitation of carbonate minerals by a microbial flocculant （MBF） produced by Bacillus mucilaginosus. MBF was added to synthetic wastewater containing different Fe^3＋ and Pb^2＋ concentrations, and the extent of flocculation was analyzed. CO2 was bubbled into the mixture of MBF and Fe^3＋/Pb^2＋ to initiate the reaction. The solid substrates were analyzed via X-ray diffraction, transmission electron microscopy and energy dispersive spectroscopy. The results showed that the removal efficiency decreased and the MBF adsorption capacity for metals increased with increasing heavy metal concentration. In the system containing MBF, metals （Fe^3＋ and Pb^2＋）, and CO2, the concentrated metals adsorbed onto the MBF combined with the dissolved CO2, resulting in oversaturation of metal carbonate minerals to form iron carbonate and lead carbonates. These results may be used in designing a method in which microbes can be utilized to combine CO2 with wastewater heavy metals to form carbonates, with the aim of mitigating environmental problems.

关键词： Bacillus mucilaginosus microbial flocculation wastewater treatment carbon dioxide fixation mineral carbonation

Tectonomicrobiology:A new paradigm for geobiological research

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2018年第4期61卷 494-498页

作者： Chuanlun ZHANG Jian LIN Sanzhong LI hailiang dong Fengping WANG Shucheng XIE Department of Ocean Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Woods Hole Oceanographic Institution Woods HoleMassachusetts 02543USA Institute of Marine Geodynamics Ocean University of ChinaQingdao 266100China State Key Laboratory of Biogeology and Environmental Geology China University ofGeosciencesBeijing 430074China Department of Geology and Environmental Earth Science Miami UniversityOxfordOhio 45056USA State Key Laboratory of Microbial Metabolism and State Key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Geomicrobiology is a sub-discipline of geobiology and emphasizes the interaction between microorganisms and their environment on Earth. There is a need to explicitly emphasize the biogeochemical processes performed by microorganisms associated with Earth's tectonic activities, especially under the framework of the modern theory of plate tectonics. Tectonomicrobiology aims to create a better synergy between microbial and active tectonic processes. This explicit synergy should also foster better communications between solid Earth scientists and life scientists in terms of holistic Earth system dynamics at both tectonic and micro-scales.

关键词： Geobiology Geomicrobiology Tectonomicrobiology Tectonic activities Microbial evolution

Occurrence of Highly Mature Organic Matter in Marine Black Shale Petroleum Source Rocks of Basal Cambrian from Northern Tarim Basin, China

在线全文

学校读者我要写书评

暂无评论

Chinese Journal Of Geochemistry 2004年第2期23卷 148-154页

作者：于炳松 hailiang dong 陈建强陈晓林梁世友 The Key Laboratory of Lithosphere Tectonics and Exploration of the Ministry of Education China University of Geosciences Beijing 100083 China Department of Geology Miami University Oxford OH 45056 USA

More and more evidence indicates that organic matter (OM) in immature organic-rich sediments and sedimentary rocks is chemically adsorbed onto the outer surfaces of minerals and into interlayer (inner) surfaces of smectitic clay minerals in the form of amorphous molecular-scale carbon. But there have been few reports about the occurrence of highly mature OM in marine black shales (petroleum source rocks). The occurrence of highly mature OM in the black shales of basal Cambrian from northern Tarim Basin is studied in this paper. Based on the comprehensive analyses of total organic carbon contents (TOC), maximum thermolysis temperatures (T-max) of OM, mineral surface areas (MSA), and scanning electronic microscopic (SEM) and transmission electronic microscopic (TEM) observations of the black shales, it is concluded that the highly mature OM in the marine black shales of the basal Cambrian from northern Tarim Basin occurs in particulates ranging in size from 1 to 5 μm in diameter. Through the contrast of the occurrence of the highly mature OM in the black shales with that of the immature ones in modern marine continental margin sediments, some scientific problems are proposed, which are worth to study further in detail.

关键词：寒武纪地层学塔里木盆地沉积学岩石学

Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2023年第6期20卷 3394-3399页

作者： YunYang Wan HongMei Mu Na Luo JianPing Yang Yan Tian Ning Hong hailiang dong National Key Laboratory of Petroleum Resources and Engineering Research Centre for Geomicrobial Resources and ApplicationBeijing Key Laboratory of Petroleum Pollution and ControlUnconventional Petroleum Research InstituteChina University of PetroleumBeijing102249China Drilling and Production Engineering Technology Department of PetroChina Liaohe Oilfield Company Panjin124010LiaoningChina Center for Geomicrobiology and Biogeochemistry Research State Key Laboratory of Biology and Environmental GeologyChina University of GeosciencesBeijing100083China

DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.

关键词： Heavy oil DNA concentration DNA purity Spectrophotometry qPCR

Distinguishing ectomycorrhizal and saprophytic fungi using carbon and nitrogen isotopic compositions

在线全文

学校读者我要写书评

暂无评论

Geoscience Frontiers 2012年第3期3卷 351-356页

作者： Weiguo Hou Bin Lian hailiang dong Hongchen Jiang Xingliang Wu State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Beijing 100083 China State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China Department of Geology and Environmental Earth Science Miami University Oxford OH 45056 USA Guizhou Academy of Sciences Guiyang 550001 China

Ectomycorrhizal fungi, a group of widespread symbiotic fungi with plant, obtain carbon source from trees and improve plant mineral nutrient uptake with their widespread hyphal network. Ectomycorrhizal fungi can be used as inoculants to improve the survival rates of plantation. Saprophytic fungi use the nutrition from the debris of plant or animals, and it is difficult to distinguish the saprophytic and ectomycorrhizal fungi by morphological and anatomic methods. In this research, the differences of stable carbon and nitrogen isotopic compositions of these fungi were analyzed. The results showed that the abundances of 13C of were higher than those of ectomycorrhizal fungi and the abundances of 15N of saprophytic fungi were lower than those of ectomycorrhizal fungi. Such differences of stable carbon and nitrogen isotopic compositions between ectomycorrhizal fungi and saprophytic fungi can be ascribed to their different nutrition sources and ecological functions. These results collectively indicate that stable carbon and nitrogen isotopic compositions are an effective proxy for distinguishing between ectomycorrhizal and saprophytic fungi.

关键词： Ectomycorrhizal fungi Saprophytic fungi Stable isotopiccompositions Ecological function

Abundance and diversity of candidate division JSl- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea

在线全文

学校读者我要写书评

暂无评论

Frontiers of Earth Science 2012年第4期6卷 373-382页

作者： Yong ZHANG Xin SU Fang CHEN Lu JIAO Hongchen JIANG hailiang dong Guochun DING State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Beijing 100083 China School of Ocean Sciences China University of Geosciences Beijing 100083 China Guangzhou Manne Geology Survey Guangzhou 510075China State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Wuhan 430074 China Department of Geology and Environmental Earth Science Miami University Oxford OH 45056 USA Julius Kfihn-lnstitut (JKl)-Federal Research Centre fbr Cultivated Plants Braunschweig 38104 Germany

Candidate division JS 1-and Chloroflexi-related bacteria are ubiquitous in various deep marine sediments worldwide, yet almost nothing is known about their abundance and diversity in cold seep sediments. Here, we investigated the abundance and diversity of JS1- and Chloroflexi-related bacteria in a cold seep marine sediment core collected from the northern South China Sea （SCS） with the employment of quantitative polymerase chain reaction （qPCR） and 16S rRNA gene phylogenetic analyses. The qPCR results showed that 16S rRNA gene copies per gram of sediments for the total bacteria and JS 1- and Chloroflexi-related bacteria were at magnitudes of 108 and 106, respectively. The relative abundance of JS 1- and Chloroflexi-related 16S rRNA genes to that of total bacteria was 0.07%-8.78% throughout the core. Phyloge- netic analyses showed that the JS-1 related clone sequences were dominant throughout the core. Our study provided insights into abundance and diversity of JS 1- and Chloroflexi-related bacteria in the northern SCS cold seep sediments.

关键词： candidate division JS 1 Chloroflexi cold seep sediments South China Sea

Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Earth Science 2012年第4期6卷 383-391页

作者： Jian YANG Hongchen JIANG Geng WU Weiguo HOU Yongjuan SUN Zhongping LAI hailiang dong State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Wuhan 430074 China State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Beijing 100083 China Qinghai Institute of Salt Lakes Chinese Academy of Sciences Xining 810008 China State Key Laboratory of Cryospheric Sciences Cold and Arid Regions Environmental and Enginee-ring Research Institute Chinese Academy of Sciences Lanzhou 730000 China Department of Geology and Environmental Earth Science Miami University Oxford OH 45056 USA

Nitrite-dependent anaerobic methane-oxidiz- ing （n-damo） bacteria and anaerobic ammonia oxidizing （anammox） bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes （with salinity of 32 and 84 g/L, respectively） on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA- based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.

关键词： anammox n-damo co-occurrence saline lakes Qinghai-Tibetan Plateau

Distribution of glycerol dialkyl glycerol tetraethers in Tibetan hot springs

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Geoscience Frontiers 2012年第3期3卷 289-300页

作者： Liu He Chuanlun L.Zhang hailiang dong Bin Fang Genhou Wang State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China Department of Marine Sciences the University of GeorgiaAthensGA 30602USA Department of Geology Miami UniversityOxfordOHUSA Geomicrobiology Laboratory the Geological Lab CenterChina University of GeosciencesBeijing 100083China School of Water Resources and Environment China University of GeosciencesBeijing 100083China

Isoprenoidal glycerol dialkyl glycerol tetraethers （iGDGTs） from the Gulu hot springs （23--83.6 ℃, pH 〉 7） and Yangbajing hot springs （80-128 ℃, pH 〉 7） were analyzed in order to investigate the distribution of archaeal lipids among different hot springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in hot springs. The sources of bacterial GDGTs （bGDGTs） in these hot springs were also investigated. The results revealed different profiles ofiGDGTs between Gulu and Yangbajing hot springs. Core iGDGTs and polar iGDGTs also presented different patterns in each hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the hot springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan hot springs.

关键词： Glycerol dialkyl glyceroltetraethers (GDGTs) Tibet Hot springs Branched and isoprenoidtetraethers (BITs) TEX86