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Formalin-induced astrocyte glutamate-glutamine cycle involved in rat spinal cord central sensitization

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Neural Regeneration Research 2009年第11期4卷 918-922页

作者： haili shen Yufeng XieO Hua Han Yayi Xia Youcheng Zhang Cuifang Wang Jing Wang Institute of Orthopaedics Second Hospital of Lanzhou University Lanzhou 730030 Gansu Province China Robarts Research Institute University of Western Ontario 100 Perth Drive London ON Canada N6A 5K8 Department of General Surgery Second Hospital of Lanzhou University Lanzhou 730030 Gansu Province China

BACKGROUND： Central sensitization, a state of increased excitability of nociceptive neurons in the spinal dorsal horn following peripheral tissue injury and/or inflammation, is an important mechanism underlying hyperalgesia and neuropathic pain. Participation of the glutamate-glutamine cycle in central sensitization of the spinal cord remains poorly understood. OBJECTIVE： To determine whether the astrocyte-neuronal glutamate-glutamine cycle is involved in formalin-induced central sensitization in the spinal cord. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Institute of Orthopedics, Second Hospital, Lanzhou University, China from September 2007 to August 2008. MATERIALS： Methionine sulfoximine （MSO, 0.1 mmol/L）, glutamine （0.25 mmol/L）, and formalin were used for this study. METHODS： A total of 43 male, Sprague Dawley rats, aged 4 months, were randomly assigned to a sham operation group （n = 6） and a model group （n = 37）. Rats in the model group received intrathecal infusion in the spinal cord. 7 days later, 37 model rats were randomly divided into PBS, MSO, glutamine, MSO ＋ glutamine and formalin subcutaneous injection alone groups. The PBS, MSO, glutamine, MSO ＋ glutamine groups were respectively intrathecally injected with PBS, MSO, glutamine, MSO ＋ glutamine （50 μL each）, and then infused with 10 μL of saline. Rats from the sham operation group were not subjected to intrathecal infusion in the spinal cord. At 15 minutes after intrathecal injection, a rat model of formalin-induced inflammatory pain was established by subcutaneous injection of 5% formalin （50 μL） in the left hindpaw. MAIN OUTCOME MEASURES： Changes in spontaneous nociceptive behavior （licking/biting or flinching） were observed following formalin injection into the rat hindpaw. RESULTS： Compared with the PBS group, duration of licking/biting was significantly shortened, and flinching frequency was significantly diminished in the MSO group （P 〈 0.05）. Com

关键词： astrocyte glutamate-glutamine cycle central sensitization inflammatory pain spinal cord

血清BMP及PPARγ水平与中轴型脊柱关节炎的骶髂关节脂肪化生的相关性研究

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兰州大学学报（医学版） 2024年第2期50卷 48-54页

作者：张宪萌谭敏李文花张瑞毛婧沈海丽兰州大学第二临床医学院甘肃兰州730030 兰州大学第二医院风湿免疫科甘肃兰州730030

目的本研究旨在调查中轴型脊柱关节炎(axSpA)患者血清中骨形态发生蛋白(BMP)和过氧化物酶体增殖激活受体γ(PPARγ)的水平与脂肪化生的相关性,进而评估脂肪化生严重程度并预测早期结构进展。方法纳入30名axSpA病例和34名健康者作为对照... 详细信息

目的本研究旨在调查中轴型脊柱关节炎(axSpA)患者血清中骨形态发生蛋白(BMP)和过氧化物酶体增殖激活受体γ(PPARγ)的水平与脂肪化生的相关性,进而评估脂肪化生严重程度并预测早期结构进展。方法纳入30名axSpA病例和34名健康者作为对照组,采用Logistic回归分析同期临床、影像资料及血清学指标。结果病例组的血清BMP4,BMP9和PPARγ水平高于健康组,差异均具有统计学意义(均P<0.001)。axSpA患者的脂肪化生程度与血清BMP4(r=0.581,P<0.001)和PPARγ(r=0.507,P=0.004)均具呈正相关关系。重度脂肪化生组的血清BMP4(17.290±7.880 pg/mL)高于无、轻度脂肪化生组(9.131±4.087 pg/mL),差异具有统计学意义(P=0.002);PPARγ在重度脂肪化生组(104.561±49.764 pg/mL)亦高于无、轻度脂肪化生组(64.392±27.007 pg/mL),差异具有统计学意义(P=0.045)。BMP4水平的升高可能是axSpA患者重度脂肪化生的危险因素(OR=1.367,95%CI:[1.026,1.822],P=0.033)。结论血清BMP4和PPARγ水平有助于评估axSpA患者的脂肪化生程度。

关键词：中轴型脊柱关节炎脂肪化生骶髂关节骨形态发生蛋白过氧化物酶体增殖激活受体

基于肠道微生物群探讨类风湿关节炎发病机制及甲氨蝶呤疗效

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华西医学 2024年第8期39卷 1302-1307页

作者：张瑞张莉毛婧李文花张宪萌沈海丽兰州大学第二临床医学院兰州730000 兰州大学第二医院风湿免疫科兰州730030

类风湿关节炎(rheumatoid arthritis,RA)是最常见的免疫介导性疾病之一,肠道微生物群与患者免疫系统之间的相互作用可能在RA的发生发展中起到一定作用。甲氨蝶呤(methotrexate,MTX)作为治疗RA的一线药物,肠道微生物及其酶产物可以通过... 详细信息

类风湿关节炎(rheumatoid arthritis,RA)是最常见的免疫介导性疾病之一,肠道微生物群与患者免疫系统之间的相互作用可能在RA的发生发展中起到一定作用。甲氨蝶呤(methotrexate,MTX)作为治疗RA的一线药物,肠道微生物及其酶产物可以通过直接和间接机制影响药物的生物利用度、临床疗效和毒性。因此了解肠道微生物群影响RA的机制、肠道微生物群对MTX疗效的影响至关重要。该文就肠道微生物群可能致RA发病的机制,以及肠道微生物群在MTX药物代谢和治疗反应中的作用机制及影响作一综述。

关键词：肠道微生物群甲氨蝶呤类风湿关节炎免疫应答

高压氧治疗联合八段锦对老年2型糖尿病合并轻度认知功能障碍患者的疗效

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江苏医药 2024年第6期50卷 614-617页

作者：沈海丽孟兆祥侯云王紫璇尹正录扬州大学医学院江苏225002 苏北人民医院康复科江苏225002 扬州市第二人民医院康复科江苏225002

目的观察高压氧治疗(HBOT)联合八段锦对老年2型糖尿病(T2DM)合并轻度认知功能障碍(MCI)患者的疗效。方法60例老年T2DM合并MCI患者随机分为对照组(30例)和观察组(30例)。对照组予以常规控制血糖、健康教育和八段锦运动,八段锦每天1次,每... 详细信息

目的观察高压氧治疗(HBOT)联合八段锦对老年2型糖尿病(T2DM)合并轻度认知功能障碍(MCI)患者的疗效。方法60例老年T2DM合并MCI患者随机分为对照组(30例)和观察组(30例)。对照组予以常规控制血糖、健康教育和八段锦运动,八段锦每天1次,每周5次;观察组在对照组基础上加用HBOT,HBOT每周3次,隔天1次;所有治疗连续4周。治疗4周后采用华山版听觉词语学习测验(AVLT-H)及蒙特利尔认知评估(MoCA)量表评价两组患者认知功能,并检测治疗前后血清IL-10、超敏C反应蛋白(hsCRP)和胰高糖素样肽1(GLP-1)水平。结果观察组治疗后AVLT-H短延迟记忆和长延迟记忆评分高于治疗前及对照组(P<0.05)。观察组治疗后MoCA量表中视空间与执行功能、延迟记忆、注意力评分和总分均高于治疗前及对照组(P<0.05)。观察组治疗后GLP-1水平高于治疗前,IL-10和hsCRP水平低于治疗前(P<0.05);观察组治疗后GLP-1水平高于对照组,IL-10和hsCRP水平低于对照组(P<0.05)。结论HBOT联合八段锦可以有效改善老年T2DM合并MCI患者的认知功能,降低进展为老年痴呆的风险。

关键词：高压氧八段锦 2型糖尿病认知功能障碍老年

血清PCSK9在系统性红斑狼疮患者心血管疾病发生风险中的预测价值

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检验医学与临床 2024年第11期21卷 1533-1537页

作者：李文花郝佳瑶谭敏毛婧张瑞张宪萌沈海丽兰州大学第二临床医学院甘肃兰州730000 兰州大学第二医院风湿免疫科甘肃兰州730030

目的探讨血清前蛋白转化酶枯草溶菌素-9(PCSK9)在系统性红斑狼疮(SLE)患者心血管疾病(CVD)发生风险中的预测价值。方法选取2023年4月至2024年1月兰州大学第二医院风湿免疫科收治的75例SLE患者作为SLE组,另选取同期在兰州大学第二医院体... 详细信息

目的探讨血清前蛋白转化酶枯草溶菌素-9(PCSK9)在系统性红斑狼疮(SLE)患者心血管疾病(CVD)发生风险中的预测价值。方法选取2023年4月至2024年1月兰州大学第二医院风湿免疫科收治的75例SLE患者作为SLE组,另选取同期在兰州大学第二医院体检的30例健康体检者作为对照组。并将SLE患者按照CVD发生风险等级分为中高危组和低危组。比较SLE组与对照组血清PCSK9水平,比较低危组、中高危组一般资料和血清学指标[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、PSCK9]。采用Pearson/Spearman相关分析SLE患者血清PCSK9水平与年龄、体质量指数(BMI)、腰围、TC、TG、HDL、LDL水平的相关性。采用多因素Logistic回归分析SLE患者有中高危CVD风险的独立危险因素。绘制受试者工作特征(ROC)曲线分析血清PCSK9对SLE患者有中高危CVD风险的预测价值。结果SLE组血清PCSK9水平明显高于对照组,差异有统计学意义(P<0.05)。中高危组有30例患者,低危组有45例患者。中高危组年龄大于低危组,动脉粥样硬化患者比例、血清PCSK9水平高于低危组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,血清PCSK9水平升高、年龄增大是SLE患者有中高危CVD风险的危险因素(P<0.05)。Pearson/Spearman相关分析结果显示,SLE患者血清PCSK9水平与年龄(r=0.228,P=0.053)、BMI(r=-0.005,P=0.963)、腰围(r=0.202,P=0.083)、TC(r_(s)=0.217,P=0.061)、TG(r_(s)=0.087,P=0.460)、HDL(r_(s)=0.495,P=0.080)、LDL(r_(s)=0.135,P=0.248)水平无相关性。ROC曲线分析结果显示,血清PCSK9预测SLE患者有中高危CVD风险的曲线下面积为0.729。结论血清PCSK9对SLE患者CVD发生风险具有一定预测价值,是潜在预测SLE患者CVD发生风险的生物标志物。

关键词：系统性红斑狼疮心血管疾病前蛋白转化酶枯草溶菌素-9 预测炎症

Shear resistance characteristics and influencing factors of root-soil composite on an alpine metal mine dump slope with different recovery periods

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Journal of Mountain Science 2024年第3期21卷 835-849页

作者： PANG Jinghao LIANG shen LIU Yabin LI shengwei WANG Shu ZHU haili LI Guorong HU Xiasong Department of Geological Engineering Qinghai UniversityXining 810016China Key Laboratory of Genozoic Resource&Environment in North Margin of the Tibetan Plateau Xining 810016China

Artificial vegetation restoration is the main measure for vegetation restoration and soil and water conservation in alpine mine dumps on the Qinghai-Tibet Plateau,***,there are few reports on the dynamic changes and the influencing factors of the soil reinforcement effect of plant species after artificial vegetation restoration under different recovery *** selected dump areas of the Delni Copper Mine in Qinghai Province,China to study the relationship between the shear strength and the peak displacement of the root-soil composite on the slope during the recovery period,and the influence of the root traits and soil physical properties on the shear resistance characteristics of the root-soil composite via in situ direct shear *** results indicate that the shear strength and peak displacement of the rooted soil initially decreased and then increased with the increase of the recovery *** shear strength of the rooted soil and the recovery period exhibited a quadratic function *** is no significant function relationship between the peak displacement and the recovery *** positive correlations(P0.05)between the shear strength of the root-soil composite and the root length density,and the root volume ratio of the coarse roots to the fine roots.A significant negative linear correlation(P0.05)with the root traits,other soil physical property indices(the moisture content and dry density of the soil),and slope *** coarse-grain content is the main factor controlling the peak displacement of the rooted soil.

关键词： Alpine mine dump Artificial vegetation restoration period Rooted soil Shear resistance characteristics Root traits Soil physical properties

Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats

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Neural Regeneration Research 2011年第35期6卷 2746-2750页

作者： Tiancheng Wang Jing Wang Bin Geng Hongyu Guo haili shen Yayi Xia Department of Neurology Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Institute of Orthopedics Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Department of Obstetrics and Gynecology Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Department of Pain Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China

Our previous behavioral studies have indicated that the astroglial glutamate-giutamine cycle is involved in the process of formalin-induced spinal cord central sensitization, but there was little morphological evidence. In this study, double-labeling immunofluorescence techniques showed that after rats were intrathecally injected with PBS and plantarly injected with formalin, glial fibrillary acidic protein （GFAP） and glutamine synthesase （GS） expression were increased and GFAP/GS coexpression was changed to include layers III and IV. After intrathecal injection of methionine sulfoximine, a GS specific inhibitor, the formalin-induced change in expression and coexpression of GFAP and GS in spinal cord dorsal horns was inhibited. The morphology, distribution and quantity of astrocytes recovered to normal levels. An intrathecal glutamine injection reversed the inhibitory effect of methionine sulfoximine. Astrocytes showed significant activation and distribution extended to layers V and VI. The present study provides morphological evidence that the astroglial glutamateglutamine cycle is involved in the process of formalin-induced spinal cord central sensitization.

关键词： astrocyte glutamate-glutamine cycle central sensitization immunohistochemistry

基于多分析方法探讨近十年T细胞对类风湿关节炎影响的可视化分析

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中国现代医生 2024年第12期62卷 1-8页

作者：苏小军朱文菊王欢何倩包强郭影可易弘沈海丽张志明甘肃省中医院风湿骨病科甘肃兰州730050 甘肃中医药大学中西医结合学院甘肃兰州730030 兰州大学第二医院风湿免疫科甘肃兰州730030

目的通过对近十年T细胞对类风湿关节炎(rheumatoid arthritis,RA)影响的文献进行多软件可视化分析,总结该领域的研究热点及前沿发展。方法在中国知网和Web of Science核心合集数据库检索2012-2022年关于T细胞对RA影响的中英文文献,运用C... 详细信息

目的通过对近十年T细胞对类风湿关节炎(rheumatoid arthritis,RA)影响的文献进行多软件可视化分析,总结该领域的研究热点及前沿发展。方法在中国知网和Web of Science核心合集数据库检索2012-2022年关于T细胞对RA影响的中英文文献,运用CiteSpace、VOSviewer软件对其发文量、作者、关键词进行分析。结果中文文献519篇,英文文献861篇,结果显示2020-2022年中文发文量缓慢增加,英文发文量整体趋势较为平稳。关键词分析显示,预测该领域的未来研究聚焦于T细胞在RA中的发病机制、RA的骨破坏机制、疾病活动度、氧化应激等方面。结论T细胞对RA影响的研究关注度一直较高,研究价值大。但因国内外研究重点存在差异,团队间应良性互动、相互交流,以期揭示RA的内在机制,为靶向治疗提供理论依据。

关键词：类风湿关节炎 T细胞 CiteSpace VOSviewer 可视化分析

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Mechanism underlying the uprooting of taproot-type shrub species in the loess area of northeastern Qinghai-Xizang Plateau, China

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Journal of Arid Land 2024年第10期16卷 1426-1443页

作者： LIANG shen WANG Shu LIU Yabin PANG Jinghao ZHU haili LI Guorong HU Xiasong School of Geological Engineering Qinghai University Key Laboratory of Genozoic Resource & Environment in North Margin of the Tibet Plateau

Characteristics of root pullout resistance determine the capacity to withstand uprooting and the slope protection ability of plants. However, mechanism underlying the uprooting of taproot-type shrub species in the loess area of northeastern Qinghai-Xizang Plateau, China remains unclear. In this study, a common taproot-type shrub, Caragana korshinskii Kom., in northeastern Qinghai-Xizang Plateau was selected as the research material. Mechanism of root-soil interaction of vertical root of C. korshinskii was investigated via a combination of a single-root pullout test and numerical simulation analysis. The results indicated that, when pulling vertically, axial force of the roots decreased with an increase in buried depth, whereas shear stress at root-soil interface initially increased and then decreased as burial depths increased. At the same buried depth, both axial force and shear stress of the roots increased with the increase in pullout force. Shear stress and plastic zone of the soil surrounding the root were symmetrically distributed along the root system. Plastic zone was located close to the surface and was caused primarily by tensile failure. In nonvertical pulling, symmetry of shear stress and plastic zone of the soil surrounding the root was disrupted. We observed larger shear stress and plastic zones on the side facing the direction of root deflection. Plastic zone included both shear and tensile failure. Axial force of the root system near the surface decreased as deflection angle of the pullout force increased. When different rainfall infiltration depths had the same vertical pulling force, root axial force decreased with the increase of rainfall infiltration depth and total root displacement increased. During rainfall infiltration, shear stress and plastic zone of the soil surrounding the root were prone to propagating deeper into the soil. These findings provide a foundation for further investigation of soil reinforcement and slope protection mechanisms

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