检索结果-南通市图书馆

Journal of Integrative Plant Biology 2013年第7期55卷 663-676页

作者： haihong shang Wei Li Changsong Zou Youlu Yuan State Key Laboratory of Cotton Biology Institute of Cotton Researchthe Chinese Academy of Agricultural Sciences

NAC domain proteins are plant-specific transcription factors known to play diverse roles in various plant developmental processes. In the present study, we performed the first comprehensive study of the NAC gene family in Gossypium raimondii Ulbr., incorporating phylogenetic, chromosomal location, gene structure, conserved motif, and expression profiling analyses. We identified 145 NAC transcription factor （NAC-TF） genes that were phylogenetically clustered into 18 distinct subfamilies. Of these, 127 NAC-TF genes were distributed across the 13 chromosomes, 80 （55%） were preferentially retained duplicates located in both duplicated regions and six were located in triplicated chromosomal regions. The majority of NAC-TF genes showed temporal-, spatial-, and tissue-specific expression patterns based on tran- scriptomic and qRT-PCR analyses. However, the expression patterns of several duplicate genes were partially redundant, suggesting the occurrence of sub-functionalization during their evolution. Based on their genomic organization, we concluded that genomic duplications contributed significantly to the expansion of the NAC-TF gene family in G. raimondii. Comprehensive analysis of their expression profiles could provide novel insights into the functional divergence among members of the NAC gene family in G. raimondii.

关键词： Cotton expression patterns NAC phylogeny structure,

Genome-Wide Analysis of the Sus Gene Family in Cotton

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2013年第7期55卷 643-653页

作者： Changsong Zou Cairui Lu haihong shang Xinrui Jing Hailiang Cheng Youping Zhang Guoli Song State Key Laboratory of Cotton Biology Institute of Cotton Researchthe Chinese Academy of Agricultural Sciences

Sucrose synthase （Sus） is a key enzyme in plant sucrose metabolism. In cotton, Sus （EC 2.***.1.13） is the main enzyme that degrades sucrose imported into cotton fibers from the phloem of the seed coat. This study demonstrated that the genomes of Gossypium arboreum L., G. raimondii Ulbr., and G. hirsutum L., contained 8, 8, and 15 Sus genes, respectively. Their structural organizations, phylogenetic relationships, and expression profiles were characterized. Comparisons of genomic and coding sequences identified multiple introns, the number and positions of which were highly conserved between diploid and allotetraploid cotton species. Most of the phylogenetic clades contained sequences from all three species, suggesting that the Sus genes of tetraploid G. hirsutum derived from those of its diploid ancestors. One Sus group （Sus I） underwent expansion during cotton evolution. Expression analyses indicated that most Sus genes were differentially expressed in various tissues and had development-dependent expression profiles in cotton fiber cells. Members of the same orthologous group had very similar expression patterns in all three species. These results provide new insights into the evolution of the cotton Sus gene family, and insight into its members＇ physiological functions during fiber growth and development.

关键词： Expression fiber Gossypium phylogenetic sucrose synthase.

A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development

在线全文

学校读者我要写书评

暂无评论

Journal of Cotton Research 2020年第3期3卷 199-209页

作者： LIU Cuixia LI Zhifang DOU Lingling YUAN Yi ZOU Changsong shang haihong CUI Langjun XIAO Guanghui Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resouce Development of Endangered Crude Drugs in the Northwest of ChinaCollege of Life SciencesShaanxi Normal UniversityXi’an 710119ShaanxiChina College of Biology Pharmacy and Food Engineering Shangluo UniversityShangluo 726000ShaanxiChina State Key Laboratory of Cotton Biology State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityKaifeng 475001HenanChina School of Chemistry and Chemical Engineering Xianyang Normal UniversityXianyang 712000ShaanxiChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China

Background:Cotton is the world’s largest and most important source of renewable natural ***1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant ***,there is no comprehensive characterization of this gene family in *** this study,32,16,and 18 BLH genes were identified from the ***,***,and *** genome,*** addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH ***:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic *** them,25 members were assigned to 15 ***,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in *** profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation *** expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not *** application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 *** levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid ***:The BLH gene family plays a very important role in many biological processes during plant growth and *** study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.

关键词： Cotton G.hirsutum BLH gene family Fiber development

Comprehensive analysis of NAC transcription factors in diploid Gossypium: sequence conservation and expression analysis uncover their roles during fiber development

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2016年第2期59卷 142-153页

作者： haihong shang Zhongna Wang Changsong Zou Zhen Zhang Weijie Li Junwen Li Yuzhen Shi Wankui Gong Tingting Chen Aiying Liu Juwu Gong Qun Ge Youlu Yuan State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences

Determining how function evolves following gene duplication is necessary for understanding gene *** factors(TFs)are a class of proteins that regulate gene expression by binding to specific cis-acting elements in the promoters of target genes,subsequently activating or repressing their *** the present study,we systematically examined the functional diversification of the NAC transcription factor(NAC-TFs)family by analyzing their chromosomal location,structure,phylogeny,and expression pattern in Gossypium raimondii(Gr)and ***(Ga).The 145 and 141 NAC genes identified in the Gr and Ga genomes,respectively,were annotated and divided into 18 subfamilies,which showed distinct divergence in gene structure and expression patterns during fiber *** addition,when the functional parameters were examined,clear divergence was observed within tandem clusters,which suggested that subfunctionalization had occurred among duplicate *** expression patterns of homologous gene pairs also changed,suggestive of the diversification of gene function during the evolution of diploid *** findings provide insights into the mechanisms underlying the functional differentiation of duplicated NAC-TFs genes in two diploid cotton species.

关键词： cotton NAC gene family phylogeny expression patterns

Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第3期90卷 837-858页

作者： Qi Zhang Pengtao Li Aiying Liu Shaoqi Li Quanwei Lu Qun Ge Junwen Li Wankui Gong Xiaoying Deng haihong shang Yuzhen Shi Youlu Yuan State Key Laboratory of Cotton Biology Key Laboratory of Biological and Genetic Breeding of CottonThe Ministry of AgricultureInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang455000China School of Biotechnology and Food Engineering Anyang Institute of TechnologyAnyang455000China Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou450001China

Cotton is the most widely cultivated commercial crop producing natural fiber around the *** a critical trait for fiber quality,fiber strength principally determined during the secondary wall thickening *** on the developed BC5F3:5 CSSLs(chromosome segment substitution lines)from Gossypium hirsutum CCRI36×*** Hai 1,the superior MBI9915 was chosen to construct the secondary segregated population BC7F2 with its recurrent parent CCRI36,which was subsequently subjected to Bulk segregant RNA-sequencing(BSR-seq)for rapid identification of candidate genes related to fiber strength.A total of 4 fiber-transcriptome libraries were separately constructed and sequenced,including two parents(CCRI36 and MBI9915)and two extreme pools at 20 DPA(days post anathesis).Through multiple comparisons,536 DEGs(differentially expressed genes)were overlapped at 20 ***-polymorphism comparison in mRNA sequences revealed 831 highly probable SNPs between two extreme pools related to fiber *** analysis was performed between two extreme pools with SNP-index *** correlated regions with 1981 annotation genes were obtained between two pools at 20 DPA,of which 12 common DEGs were similarly identified both between two parents and two *** gene(Gh_A07G0837)in the candidate region related to fiber strength was differentially expressed in both parents and extreme pools and involved in fiber strength development through reactive oxygen species(ROS)***-expression analysis of Gh_A07G0837 showed that Gh_A07G0837 may cooperate with other genes to regulate fiber *** reliability of BSR-seq results was validated by the quantitative real-time PCR(qRT-PCR)experiments on 5 common DGEs 20 ***-expressed analysis results indicated that there were some genes expressed especially low in MBI9915,resulting in good fiber *** on bulked segregant analysis on the extreme pools derived from superior CSSL population,this study indicates

关键词： Cotton Fiber strength CSSLs BSR-seq gene clone co-expressed analysis

The plasmodesmata-associated β-1,3-glucanase gene GhPdBG regulates fiber development in cotton

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2023年第6期11卷 1665-1674页

作者： Yijie Fan Shuangshuang Lin Yanhui Lyu haihong shang Youlu Yuan Zhengmin Tang Chengzhi Jiao Aiyun Chen Piyi Xing Li Zhang Yuxiao Sun Haixia Guo Tongtong Li Zhonghai Ren Fanchang Zeng State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018ShandongChina State Key Laboratory of Cotton Biology Key Laboratory of Biological and Genetic Breeding of CottonThe Ministry of AgricultureInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001HenanChina State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai’an 271018ShandongChina

Trichomes are specialized structures that originate from epidermal cells of organs in higher *** cotton fiber is a unique single-celled trichome that elongates from the seed coat ***(Gossypium hirsutum)fibers and trichomes are models for cell *** an attempt to elucidate the intercellular factors that regulate fiber and trichome cell development,we identified a plasmodesmal β-1,3-glucanase gene(designated GhPdBG)controlling the opening and closing of plasmodesmata in cotton *** and evolutionary analysis showed haplotypic variation in the promoter region of the GhPdBG gene among 352 cotton accessions,but high conservation in the coding *** was expressed predominantly in cotton fibers and localized to plasmodesmata(PD).Expression patterns of PdBG that corresponded to PD permeability were apparent during fiber development in *** and *** PdBG-mediated opening-closure of PD appears to be involved in fiber development and may account for the contrasting fiber traits of these two *** expression of GhPdBG revealed that it functions in regulating fiber and trichome length and/or density by modulating plasmodesmatal *** finding suggests that plasmodesmal targeting of GhPdBG,as a switch of intercellular channels,regulates single-celled fiber and trichome development in cotton.

关键词： Fiber/trichome β-1,3-glucanase Functional analysis Evolutional variation

QTL mapping for fiber quality and yieldrelated traits across multiple generations in segregating population of CCRI 70

在线全文

学校读者我要写书评

暂无评论

Journal of Cotton Research 2019年第2期2卷 112-121页

作者： DENG Xiaoying GONG Juwu LIU Aiying SHI Yuzhen GONG Wankui GE Qun LI Junwen shang haihong WU Yuxiang YUAN Youlu State Key Laboratory of Cotton Biology Key Laboratory of Biological and Genetic Breeding of CottonMinistry of Agriculture and Rural Affairs/Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina College of Agriculture Shanxi Agricultural UniversityTaigu 030801ShanxiChina

Background:Cotton is a significant economic crop that plays an indispensable role in many *** hirsutum *** the most important fiber crop worldwide and contributes to more than 95%of global cotto n *** stable quantitative trait locus(QTLs)controlling fiber quality and yield related traits are necessary prerequisites for marker-assisted selection(MAS).Results:A genetic linkage map was constructed with 312 simple sequence repeat(SSR)loci and 35 linkage groups using JoinMap 4.0;the map spanned 1 929.9 cM,with an average interval between two markers of 6.19 cM,and covered approximately 43.37%of the cotton genome.A total of 74 QTLs controlling fiber quality and 41 QTLs controlling yield-related traits were identified in 4 segregating *** QTLs were distributed across 20 chromosomes and collectively explained 1.01%?27.80%of the observed phenotypic *** particular,35 stable QTLs could be identified in multiple generations,25 common QTLs were con sistent with those in previous studies,and 15 QTL clusters were found in 11 chromosome ***:These studies provide a theoretical basis for improving cotton yield and fiber quality for molecular marker-assisted selection.

关键词： QTL mapping Fiber quality,Yield quality Multiple generations Upland cotton(Gossypium hirsutum L.)

Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2009年第3期19卷 347-352页

作者： Renhua Wu, Lili Wang, Zhi Wang, haihong shang, Xia Liu, Yan Zhu, Dongdong Qi, Xin Deng * Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China [a]Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Resurrection plants are tolerant to extreme dehydration and are useful model systems to study genes that play a role in drought tolerance. A gene fragment encoding a dirigent protein that is predicted to function in lignin biosynthesis was identified from leaves of the resurrection plant Boea hygrometrica via cDNA microarray screening. A cDNA, designated BhDIR1, containing the complete predicted open reading frame, was obtained by 50-RACE. BhDIR1 transcripts were found to be accumulated in response to changes in plant dehydration status, exogenously applied phytohormones and signaling molecules, and temperature stresses. BhDIR1 encodes a protein of 199 amino acids, which shows 20-40% similarity to dirigent proteins reported from other plants. BhDIR1 is predicted to contain a cleavable signal peptide at the N-terminal, and its plasma membrane/cell wall localization was confirmed using a GFP fusion protein assay. Consistent with this discovery, the acid-soluble lignin content decreased in dehydrated B. hygrometrica leaves. Taken together, our results indicate a protective role for a dirigent protein in response to drought stress by changing the physical characters of lignin which in turn is predicted to affect the mechanical strength and flexibility of the plant cell wall.

关键词： Resurrection plant Dirigent protein Drought Lignin Cell wall

Evolution of pectin synthesis relevant galacturonosyltransferase gene family and its expression during cotton fiber development

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Cotton Research 2021年第3期4卷 239-260页

作者： FAN Senmiao LIU Aiying ZOU Xianyan ZHANG Zhen GE Qun GONG Wankui LI Junwen GONG Juwu SHI Yuzhen DENG Xiaoying JIA Tingting YUAN Youlu shang haihong State Key Laboratory of Cotton Biology Key Laboratory of Biological and Genetic Breeding of CottonMinistry of Agriculture and Rural AffairsInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450000China

Background:Pectin is a key substance involved in cell wall development,and the galacturonosyltransferases(GAUTs)gene family is a critical participant in the pectin synthesis *** and comprehensive research on GAUTs has not been performed in *** of the evolution and expression patterns of the GAUT gene family in different cotton species is needed to in crease kno wledge of the functi on of pectin in cotto n fiber ***:In this study,we have identified 131 GAUT genes in the genomes of four Gossypium species(***,G barbadense,***,and ***),and classified them as GAUT-A,GAUT-B and GAUT-C,which coding probable *** them,the GAUT genes encode proteins GAUT1 to *** GAUT proteins except for GAUT7 contai n a con served glycosyl transferase family 8 domain(H-DN-A-SW-S-V-H-T-F).The conserved sequence of GAUT7 is PLN(phospholamban)02769 *** to c/s-elemet analysis,GAUT genes transcript levels may be regulated by horm ones such as JA,GA,SA,ABA,Me-JA,and IA *** evoluti on and transcription patterns of the GAUT gene family in different cotton species and the transcript levels in upland cotton lines with different fiber st「ength were *** transcript level of GhGAUT genes have been observed before 15 *** the six materials with high fiber strength,the transcription of GhGAUT genes were concentrated from 10 to 15 DPA;while the highest transcript levels in low fiber st「ength materials were detected between 5 and 10 *** results lays the foundation for future research on gene function during cotton fiber ***:The GAUT gene family may affect cotton fiber development,including fiber elongation and fiber *** the low strength fiber lines,GAUTs mainly participate in fiber elongation,whereas their major effect on cotton with high strength fiber is related to both elongation and thickening.

关键词： Cotton fiber development Pectin Galacturonosyltransferases Evolution Transcription patterns

QTL mapping for plant height and fruit branch number based on RIL population of upland cotton

在线全文

学校读者我要写书评

暂无评论

Journal of Cotton Research 2020年第1期3卷 54-62页

作者： LIU Ruixian XIAO Xianghui GONG Juwu LI Junwen ZHANG Zhen LIU Aiying LU Quanwei shang haihong SHI Yuzhen GE Qun IQBAL Muhammad Sajid CHEN Quanjia YUAN Youlu GONG Wankui State Key Laboratory of Cott on Biology/Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Department of Agronomy/Key Laboratory of Agriculture Biological Technology Xinjimng Agriculture UniversityUrumqi 830052China School of Biotechnology and Food Engineering Anyang Institute of TechndogyAnyang 455000HenanChina

Background:Plant height(PH)and fruit branch number(FBN)are important traits for improving yield and mechanical harvesting of *** order to identify genes of PH and FBN in cotton germplasms to develop superior cultivars,quantitative trait loci(QTLs)for these traits were detected based on the phenotypic evaluation data in nine environments across four locations and 4 years and a previously reported genetic linkage map of an recombinant inbred line(RIL)population of upland ***:In total,53 QTLs of PH and FBN,were identified on 21 chromosomes of the cotton genome except chromosomes c02,c09-c11,and *** PH,27 QTLs explaining 3.81%–8.54%proportions of phenotypic variance were identified on 18 chromosomes except c02,c08-c12,c15,and *** FBN,26 QTLs explaining 3.23%–11.00%proportions of phenotypic variance were identified on 16 chromosomes except c02-c03,c06,c09-c11,c17,c22-c23,and *** QTLs were simultaneously identified in at least two *** QTL clusters containing seven QTLs were identified on three chromosomes(c01,c18 and c21).Eleven QTLs were the same as previously reported ones,while the rest were newly ***:The QTLs and QTL clusters identified in the current study will be helpful to further understand the genetic mechanism of PH and FBN development of cotton and will enhance the development of excellent cultivars for mechanical managements in cotton production.

关键词： Upland cotton RIL population Agronomic traits QTL Plant height Fruiting branch number