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Particuology 2024年第9期92卷 106-112页

作者： Yiran Cheng Zhenhua Wu Boyu Zhang Jiaxu Zhang Jiafu shi Zhongyi Jiang School of Environmental Science and Engineering Tianjin UniversityTianjin300072China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China

Inactivated cell catalysis is one of several central techniques in green biomanufacturing ***,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell *** armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the ***,polymer-silica hybrid armor(PSHA)is directly generated on the surface of enzyme-containing *** branched structure of PEI enables higher porosity of cell@PSHA,exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor(SA).The electrostatic interactions(NH_(3)^(+)with O^(−))and hydrogen bonding(N⋯H or O⋯H)interactions between structural units enables higher stability of cell@PSHA,showing 3.13-fold elevation in Young's modulus compared with cell@*** a result,the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h,with an average yield of 77.76 g L^(−1).

关键词： Biomanufacturing Wholecell catalysis Polymer-silica hybrid armor Tagatose production Starch conversion

改进的采样算法与无监督聚类相结合的软件缺陷预测模型

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江西师范大学学报（自然科学版） 2024年第3期48卷 301-310页

作者：石海鹤周世文钟林辉肖正兴江西师范大学计算机信息工程学院江西南昌330022 深圳职业技术大学人工智能学院广东深圳518055

该文首先在自适应综合过采样算法ADASYN(adaptive synthetic sampling)的基础上,考虑少数类内部不同密度簇之间的连接性问题,将与采样点距离为中等的点纳入新样本生成范围,改进得到T-ADASYN过采样优化算法,有效地增加了少数类内部不同... 详细信息

该文首先在自适应综合过采样算法ADASYN(adaptive synthetic sampling)的基础上,考虑少数类内部不同密度簇之间的连接性问题,将与采样点距离为中等的点纳入新样本生成范围,改进得到T-ADASYN过采样优化算法,有效地增加了少数类内部不同密度簇的连接性,生成了分布更为均衡的数据集.然后使用基于连接的spectral clustering算法进行聚类预测操作,将过采样算法和无监督聚类相结合,提出一种新型实用的软件缺陷预测模型TA-SC(T-ADASYN+spectral clustering).以F-score为评价指标,spectral clustering为聚类模型进行验证.实验结果表明:改进的T-ADASYN过采样算法在公开的PROMISE数据集和NASA数据集上比常用的过采样算法均有6%的性能提升,且TA-SC模型在PROMISE和NASA 2个数据集上比常用聚类算法分别有3%和2%的性能提升.

关键词：软件缺陷预测类别不平衡过采样算法聚类算法无监督学习

Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters

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Synthetic and Systems Biotechnology 2024年第3期9卷 600-608页

作者： Hui Li Sheng Gao Sanyuan shi Xiaomin Zhao Haoyu Ye Yunzi Luo Department of Gastroenterology State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Georgia Tech Shenzhen Institute Tianjin UniversityTangxing Road 133Nanshan DistrictShenzhen518071China haihe Laboratory of Sustainable Chemical Transformations Tianjin300192China

Streptomyces offer a wealth of naturally occurring compounds with diverse structures,many of which possess significant pharmaceutical ***,new product exploration and increased yield of specific compounds in Streptomyces have been technically challenging due to their slow growth rate,complex culture conditions and intricate genetic *** this study,we screened dozens of Streptomyces strains inhabiting in a plant rhizosphere for fast-growing candidates,and further employed CRISPR/Cas-based engineering techniques for stepwise refinement of a particular strain,Streptomyces sp.A-14 that harbors a 7.47 Mb *** strategic removal of nonessential genomic regions and most gene clusters,we reduced its genome size to 6.13 Mb,while preserving its growth rate to the greatest *** further demonstrated that cleaner metabolic background of this engineered strain was well suited for the expression and characterization of heterologous gene clusters,including the biosynthetic pathways of actinorhodin and polycyclic tetramate ***,this streamlined genome is anticipated to facilitate directing the metabolic flux towards the production of desired compounds and increasing their yields.

关键词： Streptomyces Chassis engineering CRISPR Natural products Synthetic biology

Rapid detection of nanoplastic particles by a luminescent Tb-based coordination polymer

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Chinese Chemical Letters 2024年第5期35卷 529-533页

作者： Tiankai Sun Hui Min Zongsu Han Liang Wang Peng Cheng Wei shi Department of Chemistry College of ChemistryNankai UniversityTianjin 300071China State Key Laboratory of Separation Membranes and Membrane Processes School of Environmental Science and EngineeringTiangong UniversityTianjin 300387China Key Laboratory of Advanced Energy Materials Chemistry(MOE) Frontiers Science Center for New Organic Matter and Renewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071China haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China

Nanoplastics(NPs)in aqueous environment have become a category of emerging pollutants on account of their potential risks to both human health and *** detection of NPs is a great challenge due to the lack of sensitive and selective sensing materials with fast response time and wide sensing range of particle ***,a Tb-based coordination polymer has been synthesized for luminescent detection of nanopolystyrene with different particle sizes in aqueous solutions,showing a low limit of detection,fast response time within 10 s and high selectivity in the presence of other ***“turn-on”sensing mechanism is studied in *** work provides a facile method for the fast detection of NPs.

关键词： Coordination polymer Nano polystyrene Luminescent detection Sensing mechanism Water safety

Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

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Frontiers of Chemical Science and Engineering 2024年第4期18卷 41-48页

作者： Ziyi Chu Boyu Zhang Zhenhua Wu Jiaxu Zhang Yiran Cheng Xueying Wang Jiafu shi Zhongyi Jiang School of Environmental Science&Engineering Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their ***,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating *** structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN *** MIase@OSPN system was used for catalytic production of mannose from Dfructose.A mannose yield of 22.24% was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free *** overall catalytic efficiency(kcat/Km)of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s^(-1)·mmol^(-1)·***,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50% of the yield even after 12 cycles.

关键词： enzyme immobilization enzyme catalysis organosilica networks capsules sugar biosynthesis

A Method for Bio-Sequence Analysis Algorithm Development Based on the PAR Platform

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Big Data Mining and Analytics 2023年第1期6卷 11-20页

作者： Haipeng shi Huan Chen Qinghong Yang Jun Wang haihe shi School of Software Jiangxi Normal UniversityNanchang 330022China School of Computer and Information Engineering Jiangxi Normal UniversityNanchang 330022China

The problems of biological sequence analysis have great theoretical and practical value in modern *** solving algorithms are used for these problems,and complex similarities and differences exist among these algorithms for the same problem,causing difficulty for researchers to select the appropriate *** address this situation,combined with the formal partition-and-recur method,component technology,domain engineering,and generic programming,the paper presents a method for the development of a family of biological sequence analysis *** designs highly trustworthy reusable domain algorithm components and further assembles them to generate specifific biological sequence analysis *** experiment of the development of a dynamic programming based LCS algorithm family shows the proposed method enables the improvement of the reliability,understandability,and development efficiency of particular algorithms.

关键词： partition-and-recur(PAR) domain engineering biological sequences feature model component assembly

A Dynamic Covalent Bonding-based Nanoplatform for Intracellular Co-Delivery of Protein Drugs and Chemotherapeutics with Enhanced Anti-Cancer Effect

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Chinese Journal of Polymer Science 2024年第5期42卷 559-569,I0005页

作者： Sai-Nan Liu Jia-Hui Meng Li-Yun Cui Hua Chen Lin-Qi shi Ru-Jiang Ma Key Laboratory of Functional Polymer Materials of Ministry of Education Institute of Polymer ChemistryTianjin Key Laboratory of Functional Polymer MaterialsState Key Laboratory of Medicinal Chemical BiologyFrontiers Science Center for New Organic MatterCollege of ChemistryNankai UniversityTianjin300071China haihe Laboratory of Sustainable Chemical Transformations Tianjin300192China

Efficient intracellular delivery of protein drugs is critical for protein *** combination of protein drugs with chemotherapeutics represents a promising strategy in enhancing anti-cancer ***,co-delivery systems for efficient delivery of these two kinds of drugs are still lacking because of their different ***,we show a well-designed delivery system based on dynamic covalent bond for efficient intracellular co-delivery of ribonuclease A(RNase A)and doxorubicin(DOX).Two polymers,PEG-b-P(Asp-co-AspDA)and PAE-b-P(Asp-co-AspPBA),and two 2-acetylphenylboronic acid(2-APBA)-functionalized drugs,2-APBA-RNase A and 2-APBA-DOX,self-assemble into mixed-shell nanoparticles(RNase A/DOX@MNPs)via dynamic phenylboronic acid(PBA)-catechol bond between PBA and dopamine(DA)*** PBA-catechol bond endows the nanoparticles with high stability and excellent stimulus-responsive drug release *** the slight acidic environment at tumor tissue,RNase A/DOX@MNPs are positively charged,promoting their *** cellular uptake into endosome,further protonation of PAE chains leads to the rupture of endosomes because of the proton sponge effect and the cleavage of PBA-catechol bond promotes the release of two *** cytoplasm,the high level of GSH removed the modification of 2-APBA on *** restored RNase A and DOX show a synergistic and enhanced antic-cancer *** system may be a promising platform for intracellular co-delivery of protein drugs and chemotherapeutics.

关键词： Drug co-delivery Combination therapy Dynamic covalent bond

Micro-nanoarchitectonic of aluminum-hydrogel propellant with static stability and dynamic rheology

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Frontiers of Chemical Science and Engineering 2024年第4期18卷 87-96页

作者： Huiyan Guo Huaiyu Li Hongzhi Zhang Lun Pan Chengxiang shi Kang Xue Xiangwen Zhang Ji-Jun Zou Key Laboratory for Advanced Fuel and Propellant of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

The aluminum-water system is a promising propellant due to high energy and low signal characteristics,and the gel form is easier to store and *** this work,hydrogels of water and aluminum particles were prepared using the low-molecular-weight gellant *** various physical properties of gel systems,including the water loss rate,phase transition temperature,and centrifugal stability at different gellant and aluminum contents,were *** properties were assessed through shear thinning tests,thixotropy tests,strain sweep analysis,and frequency sweep *** microstructure of the gel was obtained through scanning electron microscopy *** results show that the aluminum-hydrogel network structure is composed of micron-scale aluminum and agarose nanosheets,and the unique micro-nanostructure endows the gel with excellent mechanical strength and thermal stability,which improve with increasing gellant and aluminum ***,the gel with 2% agarose and 20% aluminum had the best performance;the storage modulus reached 90647 Pa,which was within the linear viscoelastic region,and the maximum withstand pressure was 111.2 kPa,which was 118.8% greater than that of the pure ***,the gel demonstrates remarkable shear thinning behavior and can undergo gel-sol transformation upon shearing or heating to exceeding 114℃.

关键词： aluminum-water propellant gelled propellant rheological property mechanical strength

Electrostatic and hydrophobic interaction cooperative nanochaperone regulates protein folding

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Aggregate

Aggregate 2024年第1期5卷 419-426页

作者： Xiaohui Wu Fei Deng Yujie Chen Mengchen Xu Feihe Ma Linqi shi Key Laboratory of Functional Polymer Materials of Ministry of Education State Key Laboratory of Medicinal Chemical BiologyInstitute of Polymer Chemistry and College of ChemistryNankai UniversityTianjinP.R.China School of Materials Science and Engineering and State Key Laboratory of Separation Membranes and Membrane Processes Tiangong UniversityTianjinP.R.China

Natural molecular chaperones utilize spatially ordered multiple molecular forces to effectively regulate protein ***,synthesis of such molecules is a big *** concept of“aggregate science”provides insights to construct chemical entities(aggregates)beyond molecular levels to mimic both the structure and function of natural *** by this concept,herein we fabricate a novel multi-interaction(i.e.,electrostatic and hydrophobic interaction)cooperative nanochaperone(multi-co-nChap)to regulating protein *** multi-co-nChap is fabricated by rationally introducing electrostatic interactions to the surface(corona)and confined hydrophobic microdomains(shell)of traditional single-hydrophobic interaction *** demonstrate that the corona electrostatic attraction facilitates the diffusion of clients into the hydrophobic microdomains,while the shell electrostatic interaction balances the capture and release of *** finely synergizing corona electrostatic attraction with shell electrostatic repulsion and hydrophobic interaction,the optimized multi-co-nChap effectively facilitated de novo folding of nascent ***,the synergy between corona electrostatic attraction,shell electrostatic attraction and shell hydrophobic interaction significantly enhanced the capability of multi-co-nChap to protect native proteins from denaturation at harsh *** work provides important insights for understanding and design of nanochaperone,which is a kind of ordered aggregate with chaperone-like activity that beyond the level of single molecule.

关键词： chaperone-like activity cooperative effect nanochaperones ordered aggregates protein folding