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分泌性中耳炎动物模型的探索及进展

中华耳鼻咽喉头颈外科杂志 2024年第4期59卷 405-410页

作者：万芊时海波上海交通大学医学院附属第六人民医院耳鼻咽喉头颈外科上海200233

分泌性中耳炎(secretory otitis media,SOM),亦称渗出性中耳炎(otitis media effusion,OME),是以传导性聋及鼓室积液为主要特征的中耳非化脓性炎性疾病。OME为临床常见疾病,在学龄前儿童中发病率尤高。OME病因复杂,在基础研究中难以获... 详细信息

分泌性中耳炎(secretory otitis media,SOM),亦称渗出性中耳炎(otitis media effusion,OME),是以传导性聋及鼓室积液为主要特征的中耳非化脓性炎性疾病。OME为临床常见疾病,在学龄前儿童中发病率尤高。OME病因复杂,在基础研究中难以获得与人类OME发病高度一致的动物模型,相关病理生理机制研究和治疗方法受限。本文归纳了OME造模动物的种类,并从咽鼓管功能障碍、感染及免疫反应这3个可能与OME相关的病因入手,阐述了手术致咽鼓管功能障碍、中耳鼓室注射诱导、基因变异3大类OME动物模型,对其优缺点及适用情况进行了讨论。

关键词：渗出性中耳炎病理生理机制咽鼓管功能障碍传导性聋分泌性中耳炎鼓室积液动物模型基因变异

新发展阶段扶贫项目资产管理典型模式及启示建议

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湖南农业大学学报（社会科学版） 2024年第3期25卷 86-94页

作者：李芸施海波吕开宇中国农业科学院农业经济与发展研究所北京100081 安徽省农业科学院农业经济与信息研究所安徽合肥230001

选取安徽省W县、陕西省F县和四川省N县作为样本进行考察,研究表明安徽省W县的“严把五关”模式、陕西省F县的“四五三”模式、四川省N县的“五字诀”模式因地制宜探索摸清底数、确权登记、落实管理责任、规范管护运营与收益分配、严格... 详细信息

选取安徽省W县、陕西省F县和四川省N县作为样本进行考察,研究表明安徽省W县的“严把五关”模式、陕西省F县的“四五三”模式、四川省N县的“五字诀”模式因地制宜探索摸清底数、确权登记、落实管理责任、规范管护运营与收益分配、严格资产处置等扶贫项目资产管理机制,取得了显著成效。进入新发展阶段,扶贫项目资产管理面临着“多头管”“理不清”“风险大”等现实问题,应加强顶层设计,多方协同齐抓共管;加强运营管护,提高资产绩效;加强分类管理,提升管理的精准性;防控多重风险,切实保护资产安全。

关键词：扶贫项目资产资产管理典型模式启示建议新发展阶段

Techniques for postmortem tenderisation in meat processing:effectiveness,application and possible mechanisms

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Food Production, Processing and Nutrition

Food Production, Processing and Nutrition 2021年第1期3卷 237-262页

作者： haibo shi Fereidoon Shahidi Jiankang Wang Yan Huang Ye Zou Weimin Xu Daoying Wang Institute of Agricultural Products Processing Jiangsu Academy of Agricultural SciencesNanjing 210014People’s Republic of China Department of Biochemistry Memorial University of NewfoundlandA1B3X9St.John’sNLCanada School of Food and Biological Engineering Shanxi University of Science and TechnologyXi’an 710021People’s Republic of China

Developing efficient and promising tenderising techniques for postmortem meat is a heavily researched topic among meat scientists as consumers are willing to pay more for guaranteed tender meat.However,emerging tenderising techniques are not broadly used in the meat industry and,to some degree,are controversial due to lack of theoretical support.Thus,understanding the mechanisms involved in postmortem tenderisation is essential.This article first provides an overview of the relationship of ageing tenderisation and calpain system,as well as proteomics applied to identify protein biomarkers characterizing tenderness.In general,the ageing tenderisation is mediated by multiple biochemical activities,and it can exhibit better palatability and commercial benefit by combining other interventions.The calpain system plays a key role in ageing tenderisation functions by rupturing myofibrils and regulating proteolysis,glycolysis,apoptosis and metabolic modification.Additionally,tenderising techniques from different aspects including exogenous enzymes,chemistry,physics and the combined methods are discussed in depth.Particularly,innovation of home cooking could be recommended to prepare relatively tender meat due to its convenience and ease of operation by consumers.Furthermore,the combined interventions provide better performance in controlled tenderness.Finally,future trends in developing new tenderising techniques,and applied consideration in the meat processing industry are proposed in order to improve meat quality with higher economical value.

关键词： Postmortem tenderisation Ageing Emerging technique Combination intervention Functionary mechanism

Cisplatin-induced vestibular hair cell lesion-less damage at high doses

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Journal of Otology 2018年第4期13卷 115-121页

作者： Dalian Ding Haiyan Jiang Jianhui Zhang Xianrong Xu Weidong Qi haibo shi Shankai Yin Richard Salvi Center for Hearing and Deafness University at BuffaloUSA Department of Otolaryngology Head and Neck Surgery The Third People’s Hospital of ChengduChina Air Force General Hospital BeijingChina Department of Otolaryngology Head and Neck Surgery Huashan HospitalFudan UniversityShanghaiChina Department of Otorhinolaryngology Affiliated Sixth People’s Hospital of Shanghai Jiaotong UniversityShanghaiChina Department of Audiology and Speech-Language Pathology Asia UniversityChina

Cisplatin, a widely used anticancer drug, damages hair cells in cochlear organotypic cultures at low doses, but paradoxically causes little damage at high doses resulting in a U-shaped dose-response function. To determine if the cisplatin dose-response function for vestibular hair cells follows a similar pattern, we treated vestibular organotypic cultures with doses of cisplatin ranging from 10 to 1000 μM. Vestibular hair cell lesions progressively increased as the dose of cisplatin increased with maximum damage occurring around 50-100 μM, but the lesions progressively decreased at higher doses resulting in little hair cell loss at 1000 μM. The U-shaped doseresponse function for cisplatin-treated vestibular hair cells in culture appears to be regulated by copper transporters, Ctrl, ATP7A and ATP7B, that dose-dependently regulate the uptake, sequestration and extrusion of cisplatin.

关键词： Cisplatin Ototoxicity Copper transporters Vestibular organotypic cultures

The molecular,electrophysiological,and structural changes in the vestibular nucleus during vestibular compensation:a narrative review

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Journal of Bio-X Research 2021年第4期4卷 158-162页

作者： Pengjun Wang Wen Lu Yini Li haibo shi Shankai Yin Department of Otorhinolaryngology-Head&Neck Surgery Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong UniversityShanghaiChina

The vestibular system involves high-level sensory and cognitive processes,such as spatial perception,balance control,visual stability,and emotional control.Vestibular dysfunction can induce vertigo,blurred vision,postural imbalance,walking instability,and spatial discomfort,which causes serious damage to health.It has long been known that after peripheral vestibular lesion,vestibular dysfunction may spontaneously recover.This is known as vestibular compensation.However,at least 20%to 30%of patients with vestibular disorders cannot yield vestibular compensation and remain with vestibular dysfunction for the rest of their lives.The exploration of the biological characteristics and regulatory factors of the loss and reestablishment of vestibular function will establish a new understanding of the mechanism of vestibular compensation and provide new tools and strategies for promoting vestibular rehabilitation.We aim to comprehensively review the mechanism of vestibular compensation and discuss future directions in this field.

关键词： mechanism molecular biology neurogenesis vestibular compensation vestibular nucleus

Antioxidative stress-induced damage in cochlear explants

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Journal of Otology 2020年第1期15卷 36-40页

作者： Dalian Ding Jianghui Zhang Fang Liu Peng Li Weidong Qi Yazhi Xing haibo shi Haiyan Jiang Hong Sun Shankai Yin Richard Salvi Center for Hearing and Deafness State University of New York at BuffaloUnited States The Third People's Hospital of Chengdu ChengduChina Beijing Hospital and National Center of Gerontology Department of OtolaryngologyBeijingChina The Third Affiliated Hospital of Sun Yat-Sen University GuangzhouChina Huashan Hospital Fudan UniversityShanghaiChina Shanghai Six People's Hospital Shanghai Jiao Tong UniversityShanghaiChina Xiangya Hospital Central South UniversityChangshaHunanChina

The imbalance of reactive oxygen species and antioxidants is considered to be an important factor in the cellular injury of the inner ear. At present, great attention has been placed on oxidative stress. However,little is known about fighting oxidative stress. In the current study, we evaluated antioxidant-induced cochlear damage by applying several different additional antioxidants. To determine whether excessive antioxidants can cause damage to cochlear cells, we treated cochlear explants with 50 m M M40403, a superoxide dismutase mimetic, 50 m M coenzyme Q-10, a vitamin-like antioxidant, or 50 m M d-methionine, an essential amino acid and the important antioxidant glutathione for 48 h. Control cochlear explants without the antioxidant treatment maintained their normal structures after incubation in the standard serum-free medium for 48 h, indicating the maintenance of the inherent oxidative and antioxidant balance in these cochlear explants. In contrast, M40403 and coenzyme Q-10-treated cochlear explants displayed significant hair cell damage together with slight damage to the auditory nerve fibers.Moreover, d-methiodine-treated explants exhibited severe damage to the surface structure of hair cells and the complete loss of the spiral ganglion neurons and their peripheral fibers. These results indicate that excessive antioxidants are detrimental to cochlear cells, suggesting that inappropriate dosages of antioxidant treatments can interrupt the balance of the inherent oxidative and antioxidant capacity in the cell.

关键词： Antioxidative stress Cochlea M40403 Coenzyme Q-10 D-methiodine

A zebrafish model for hearing loss and regeneration induced by blast wave

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Journal of Bio-X Research 2019年第2期2卷 87-97页

作者： Jiping Wang Zheng Yan Yazhi Xing Ke Lai Jian Wang Dongzhen Yu haibo shi Shankai Yin Department of Otolaryngology Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University Shanghai Key Laboratory of Sleep Disordered Breathing National Key Laboratory of Science and Technology on Underwater Acoustic Antagonizing Shanghai Marine Electronic Equipment Research Institute ShanghaiChina School of Human Communication Disorder Dalhousie UniversityHalifaxNova ScotiaCanada

Zebrafish have the potential to regrow injured organs and tissues,but their use as a model for hearing regeneration following blast injury has never been reported.In this study,zebrafish were exposed to a blast wave produced by an underwater blast wave generator.The first peak sound pressures produced by this generator were up to 224dB and 160kPa,measured at 25cm away from the machine.Zebrafish hearing sensitivity was examined by analyzing auditory evoked potentials from 1 to 35 days post blast wave exposure.Cell death and cell proliferation in inner ear organs,including the saccule,lagena,and utricle,were investigated using a terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling assay,and cell proliferation assay using 5-ethynyl-20-deoxyuridine,respectively.Significant differences in auditory evoked potential thresholds were observed between exposed and control groups,demonstrating both blast wave-induced hearing loss and recovery of hearing sensitivity.An apoptosis assay revealed significantly increased numbers of terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labelingpositive cells in the inner ear sensory epithelia of exposed groups compared with the control group.However,numbers of 5-ethynyl-20-deoxyuridine-positive cells in the inner ear of exposed groups recovered to a normal level within 10 post blast wave exposure.Furthermore,blast wave exposure caused brain injury with increased cell apoptosis and decreased neurogenesis.Compared with drug or noise-induced zebrafish models,our blast wave-induced model elicited more serious hearing loss phenotypes,which required more time to return to a normal level.Overall,this zebrafish model can provide a reliable animal model for both hearing loss and regeneration research.The study was approved by the Shanghai 6th Hospital Animal Care and Use Committee,China(approval No.2017-0196)on February 28,2017.

关键词： blast wave zebrafish hearing loss brain injury auditory evoked potential hair cell apoptosis regeneration

补肾解毒方联合耳穴压豆对晚期结直肠癌患者癌性疼痛及生活质量的影响

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实用临床医药杂志 2024年第7期28卷 41-44页

作者：石建娜周新东孙科石海波陈霞江苏省无锡市锡山区中医医院肿瘤科江苏无锡214194

目的探讨补肾解毒方联合耳穴压豆对晚期结直肠癌患者癌性疼痛及生活质量的影响。方法将2019年6月—2023年5月本院收治的120例晚期结直肠癌患者纳入本研究,并将其随机分为2组。对照组(60例)行常规护理和“三阶梯”镇痛法干预疼痛,观察组... 详细信息

目的探讨补肾解毒方联合耳穴压豆对晚期结直肠癌患者癌性疼痛及生活质量的影响。方法将2019年6月—2023年5月本院收治的120例晚期结直肠癌患者纳入本研究,并将其随机分为2组。对照组(60例)行常规护理和“三阶梯”镇痛法干预疼痛,观察组(60例)在对照组基础上行补肾解毒方联合耳穴压豆治疗,2组均持续治疗1个月。比较2组患者治疗前后数字评分量表(NRS)评分、治疗效果、生活质量量表(QOL)评分、不良反应发生情况,检测血清中P物质(SP)、β内啡肽(β-EP)、前列腺素E_(2)(PGE_(2))水平。结果治疗后,观察组NRS评分以及SP、β-EP和PGE_(2)水平分别为(2.23±0.64)分、(11.51±2.64)ng/mL、(78.64±9.46)ng/L、(41.90±6.32)ng/L,分别低于对照组的(2.87±1.25)分、(15.64±2.42)ng/mL、(101.29±11.26)ng/L、(78.63±10.17)ng/L,差异有统计学意义(P0.05)。结论补肾解毒方联合耳穴压豆可明显改善晚期结直肠癌患者的癌性疼痛,提高生活质量,且安全性较好。

关键词：补肾解毒方耳穴压豆晚期结直肠癌癌性疼痛生活质量

Efficient unequal error protection for online fountain codes

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Journal of Systems Engineering and Electronics 2024年第2期35卷 286-293页

作者： shi Pengcheng WANG Zhenyong LI Dezhi LYU haibo School of Electronics and Information Engineering Harbin Institute of TechnologyHarbin 150001China Shenzhen Academy of Aerospace Technology Shenzhen 518057China

In this paper,an efficient unequal error protection(UEP)scheme for online fountain codes is proposed.In the buildup phase,the traversing-selection strategy is proposed to select the most important symbols(MIS).Then,in the completion phase,the weighted-selection strategy is applied to provide low overhead.The performance of the proposed scheme is analyzed and compared with the existing UEP online fountain scheme.Simulation results show that in terms of MIS and the least important symbols(LIS),when the bit error ratio is 10-4,the proposed scheme can achieve 85%and 31.58%overhead reduction,respectively.

关键词： online fountain code random graph unequal error protection(UEP) rateless code