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Drying-rewetting cycles reduce bacterial diversity and carbon loss in soil on the Loess Plateau of China

Pedosphere 2023年第6期33卷 838-848页

作者： Panpan JIAO haibing xiao Zhongwu LI Lei YANG Peng ZHENG State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Research Center of Soil and Water Conservation and Ecological EnvironmentChinese Academy of Sciences and Ministry of EducationYangling 712100China Institute of Soil and Water Conservation Chinese Academy of Sciences and Ministry of Water ResourcesYangling 712100China University of Chinese Academy of Sciences Beijing 100049China State Environmental Protection Key Laboratory of Soil Health and Green Remediation Huazhong Agricultural UniversityWuhan 430070China College of Resources and Environmental Sciences Hunan Normal UniversityChangsha 410081China Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China

With global climate change, soil drying-rewetting(DRW) events have intensified and occurred frequently on the Loess Plateau of China. However, the extent to which the DRW cycles with different wetting intensities and cycle numbers alter microbial community and respiration is barely understood. Here,indoor DRW one and four cycles treatments were implemented on soil samples obtained from the Loess Plateau, involving increase of soil moisture from10% water-holding capacity(WHC) to 60% and 90% WHC(i.e., 10%–60% and 10%–90% WHC, respectively). Constant soil moistures of 10%, 60%,and 90% WHC were used as the controls. The results showed that bacterial diversity and richness decreased and those of fungi remained unchanged under DRW treatments compared to the controls. Under all moisture levels, Actinobacteriota and Ascomycota were the most dominant bacterial and fungal phyla,respectively. The bacterial network was more complex than that of fungi, indicating that bacteria had a greater potential for interaction and niche sharing under DRW treatments. The pulse of respiration rate declined as the DRW cycle increased under 10%–60% WHC, but remained similar for different cycles under 10%–90% WHC. Moreover, the DRW treatments reduced the overall carbon loss, and the direct carbon release under 10%–60% WHC was larger than that under 10%–90% WHC. The cumulative CO_(2) emissions after four DRW cycles were significantly positively correlated with microbial biomass carbon and negatively correlated with fungal richness(Chao 1).

关键词： bacterial network cumulative CO_(2)emissions fungal richness microbial biomass carbon microbial community respiration rate soil moisture

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Genome Analysis of Dasineura jujubifolia Toursvirus 2,A Novel Ascovirus

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Virologica Sinica 2020年第2期35卷 134-142页

作者： Jun Wang Minglu Yang haibing xiao Guo-Hua Huang Fei Deng Zhihong Hu State Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Tarim University Alar 843300China Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Institute of VirologyHunan Agricultural UniversityChangsha 410128China

So far,ascoviruses have only been identified from Lepidoptera host insects and their transmission vectors-endoparasitic ***,we reported the first finding of a complete novel ascovirus genome from a Diptera insect,Dasineura ***,sequence fragments with homology to ascoviruses were incidentally identified during metagenomic sequencing of the mitochondria of ***(Cecidomyiidae,Diptera)which is a major pest on Ziziphus *** a full circular viral genome was assembled from the metagenomic data,which has an A+T percentage of 74%and contains 142,600 bp with 141 open reading frames(ORFs).Among the 141 ORFs,37 were conserved in all sequenced ascoviruses(core genes)including proteins predicted to participate in DNA replication,gene transcription,protein modification,virus assembly,lipid metabolism and ***-gene families including those encode for baculovirus repeated open reading frames(BROs),myristylated membrane proteins,RING/U-box E3 ubiquitin ligases,and ATP-binding cassette(ABC)transporters were found in the virus *** analysis showed that the newly identified virus belongs to genus Toursvirus of Ascoviridae,and is therefore named as Dasineura jujubifolia toursvirus 2(DjTV-2a).The virus becomes the second reported species of the genus after Diadromus pulchellus toursvirus 1(DpTV-1a).The genome arrangement of DjTV-2a is quite different from that of DpTV-1a,suggesting these two viruses separated in an early time of *** results suggest that the ascoviruses may infect a much broader range of hosts than our previous knowledge,and shed lights on the evolution of ascoviruses and particularly on that of the toursviruses.

关键词： Dasineura jujubifolia toursvirus 2(DjTV-2a) Ascovirus Toursvirus

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Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism

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Frontiers of Medicine 2014年第4期8卷 456-463页

作者： Runlin Shi haibing xiao Tao Yang Lei Chang Yuanfeng Tian Bolin Wu Hua Xu Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

microRNAs （miRNAs） have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c （miR-200c） on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200e compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR- 200c could inhibit migration and invasion by epithelial-mesenchymal transition.

关键词： miR-200c proliferation migration invasion prostate cancer Du145 cell ZEB1

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Folic acid-modified Exosome-PH20 enhances the efficiency of therapy via modulation of the tumor microenvironment and directly inhibits tumor cell metastasis

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Bioactive Materials 2021年第4期6卷 963-974页

作者： Chunxiang Feng Zhiyong Xiong Cheng Wang Wen xiao haibing xiao Kairu Xie Ke Chen Huageng Liang xiaoping Zhang Hongmei Yang Department of Pathogenic Biology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

High accumulation of hyaluronan(HA)in the tumor microenvironment leads to an increase in the interstitial pressure and reduction perfusion of ***,high molecular-weight(HMW)-HA suppresses M1 macrophage polarization,enhances M2 polarization,and induces *** treatment have attempted to decrease the quantity of HA in ***,hyaluronidase-driven HA degradation driven accelerates tumor cell metastasis,which is a major cause of mortality in cancer ***,we designed a novel exosome-based drug delivery system(DDS),named Exos-PH20-FA,using genetic engineering to express human hyaluronidase(PH20)and self-assembly techniques to modify the exosomes with folic acid(FA).Our results show that Exos-PH20-FA degraded HMW-HA to low-molecular-weight(LMW)-***,LMW-HA polarized macrophages to the M1 phenotype and reduced the number of relevant immunosuppressive immunocytes which changed the immune microenvironment from an immunosuppressive to immunosupportive ***,we demonstrated Exos-PH20-FA directly reduced hyaluronidase-induced metastasis of tumor *** tumor treatment also allowed an enhanced delivery of chemotherapy by tumor-targeting effect with FA *** findings indicate that Exos-PH20-FA improves tumor treatment efficiency and reduces the side effects of hyaluronidase treatment,namely tumor cell *** all-in-one exosome-based HA targeting DDS maybe a promising treatment that yields more efficient and safer results.

关键词： Hyaluronidase Exosomes Tumor microenvironment Metastasis Tumor immunotherapy

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Laser polishing of a high-entropy alloy manufactured by selective laser melting

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Frontiers of Mechanical Engineering 2024年第5期19卷 73-86页

作者： xiaojun TAN haibing xiao Zihong WANG Wei ZHANG Zhijuan SUN Xuyun PENG Zhongmin LIU Liang GUO Qingmao ZHANG Intelligent Manufacturing and Equipment School Shenzhen Institute of Information TechnologyShenzhen 518172China Sino-German Intelligent Manufacturing School Shenzhen City PolytechnicShenzhen 518116China Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices School of Information and Optoelectronic Science and EngineeringSouth China Normal UniversityGuangzhou 510006China Guangdong Provincial Key Laboratory of Industrial Ultrashort Pulse Laser Technology Shenzhen 518055China College of Materials Science and Engineering Chongqing UniversityChongqing 400045China

The selective laser melting(SLM)technique applied to high-entropy alloys(HEAs)has attracted considerable attention in recent ***,its practical application has been restricted by poor surface *** this study,the capability of laser polishing on the rough surface of a Co-free HEA fabricated using SLM was *** show that the initial SLM-manufactured(as-SLMed)surface of the Co-free HEA,with a roughness exceeding 3.0μm,could be refined to less than 0.5μm by laser ***,the microstructure,microhardness,and wear resistance of the laser-polished(LP-ed)zone were *** indicate that compared with the microhardness and wear resistance of the as-SLMed layer,those of the LP-ed layer decreased by 4%and 11%,respectively,because of the increase in grain size and reduction of the BCC *** study shows that laser polishing has an excellent application prospect in surface improvement of HEAs manufactured by SLM.

关键词： laser polishing selective laser melting high-entropy alloy surface roughness mechanical performance

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基于能量耗损的发动机故障诊断方法研究

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润滑与密封 2011年第5期36卷 1-3,58页

作者：谢小鹏肖海兵冯伟华南理工大学汽车摩擦学与故障诊断研究所广东广州510640 广州机械科学研究院广东广州510700

针对发动机在发生故障时都伴随能量耗损变化,提出一种基于能量耗损的发动机故障诊断方法。基于发动机系统输入输出的能量分析,建立发动机系统的能量监测模型;根据输入的流量与输出的功率,建立发动机系统的能量耗损模型;根据能量耗损机制... 详细信息

针对发动机在发生故障时都伴随能量耗损变化,提出一种基于能量耗损的发动机故障诊断方法。基于发动机系统输入输出的能量分析,建立发动机系统的能量监测模型;根据输入的流量与输出的功率,建立发动机系统的能量耗损模型;根据能量耗损机制,分析发动机运动副的摩擦与振动能量耗损诊断方法,建立摩擦和振动信息与能量耗损模型。以发动机活塞磨损故障为例,从振动耗能的角度,通过对测振信号的小波分析,得到能量耗损变化,诊断出活塞的磨损状态,验证了基于能量耗损的发动机故障诊断方法是可行的。

关键词：能量耗损摩擦磨损振动故障发动机故障诊断

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A Meta-analysis of Mood Stabilizers for Alzheimer's Disease

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第5期30卷 652-658页

作者：肖海兵苏颖曹旭孙圣刚梁直厚 Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology the Central Hospital of Wuhan

The objective of this study was to assess the clinical evidence for or against mood stabilizers as a treatment for Alzheimer’s disease (AD).We searched 5 databases from their inception to January *** randomized clinical trials of mood stabilizers to treat human patients suffering from AD were *** trials assessed the effectiveness of mood stabilizers as an adjunct treatment to conventional anti-dementia drugs on behavioral and psychological symptoms, especially on *** quality was assessed using the Jadad *** results suggested a significant effect in favor of placebo on the Mini-Mental Status Examination [n=270, weight mean difference (WMD), -0.89; 95% confidence intervals (CIs) -1.69 to -0.09, P=0.03] and on the Neuropsychiatric Inventory total (NPI total) (n=51, WMD, 3.71; 95% CIs 0.15 to 7.26, P=0.04).There were no significant differences in change scores on total Brief Psychiatric Rating Scale (BPRS total), NPI/BPRS agitation, Cohen-Mansfield Agitation Inventory total and Physical Self Maintenance Scale between mood stabilizers and *** one of these studies was free of methodological limittions (Jadad score=5).In conclusion, based on the existing evidence, mood stabilizers are ineffective or even harmful as a treatment for AD.

关键词： Alzheimer’s disease behavioral and psychological symptoms mood stabilizers meta-analysis

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转sck/cry1Ac基因稻谷对米蛾幼虫解毒酶系的影响

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植物保护 2012年第4期38卷 21-26页

作者：肖海兵刘映红刘旭肖珍珍杨璐龙洁西南大学植物保护学院昆虫学及害虫控制工程重庆市重点实验室重庆400716

采用酶活力测定及酯酶同工酶电泳方法研究米蛾(Corcyra cephalonica)幼虫取食表达SCK/Cry1Ac毒蛋白的转基因稻谷后体内重要解毒酶活力的变化,明确其代谢毒蛋白的主要酶类。结果表明:取食转Bt基因稻谷米蛾幼虫体内的α-乙酸萘酯酶、碱性... 详细信息

采用酶活力测定及酯酶同工酶电泳方法研究米蛾(Corcyra cephalonica)幼虫取食表达SCK/Cry1Ac毒蛋白的转基因稻谷后体内重要解毒酶活力的变化,明确其代谢毒蛋白的主要酶类。结果表明:取食转Bt基因稻谷米蛾幼虫体内的α-乙酸萘酯酶、碱性磷酸酯酶的活力在24～72h显著低于对照;酸性磷酸酯酶活力在12、48h和72h时也显著低于对照;而α-乙酸萘酯羧酸酯酶和乙酰胆碱酯酶的活力显著高于对照。谷胱甘肽-S-转移酶的活性除饲喂24h的处理外,其他处理与对照无显著差异。此外处理72h后米蛾幼虫体内酯酶同工酶谱有4条酯酶酶带,4种酯酶Est1、Est2、Est3、Est4的活性均受到不同程度的抑制,酯酶Est4的抑制效果最明显。这些解毒酶和酯酶同工酶直接参与Bt毒蛋白的代谢作用。

关键词：转Bt基因稻谷 SCK/Cry1Ac毒蛋白米蛾解毒酶酯酶同工酶

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基于能量耗损的齿轮摩擦学系统故障规则研究

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润滑与密封 2014年第3期39卷 4-6,13页

作者：黄恒谢小鹏肖海兵刘文涛华南理工大学汽车摩擦学与故障诊断研究所广东广州510640 广州中船船用柴油机有限公司广东广州510250

针对齿轮在发生故障时输入能量耗损会发生变化这一现象,提出一种基于能量耗损的齿轮摩擦学系统故障诊断方法并建立故障规则。研究齿轮摩擦学系统与能量耗损理论;设计齿轮能量监测试验台,获取齿轮能量耗损信息;采用神经网络与模糊理论规... 详细信息

针对齿轮在发生故障时输入能量耗损会发生变化这一现象,提出一种基于能量耗损的齿轮摩擦学系统故障诊断方法并建立故障规则。研究齿轮摩擦学系统与能量耗损理论;设计齿轮能量监测试验台,获取齿轮能量耗损信息;采用神经网络与模糊理论规则提取方法,选择输入功率作为齿轮能量耗损信息,对模糊控制规则进行修改,建立能量耗损信息的齿轮模糊的故障规则。研究表明,基于能量耗损的齿轮故障规则是有效准确的,为实现基于能量耗损的故障诊断奠定了基础。

关键词：能量耗损齿轮故障诊断模糊理论故障规则

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基于水文连通性的流域输沙量评估——以堵河流域为例

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水土保持研究 2022年第3期29卷 64-70页

作者：张媛平刘成帅肖海兵王剑史志华华中农业大学资源与环境学院武汉430070

水文连通性是指泥沙、营养盐等各种物质以水为载体,在空间异质景观或斑块间进行迁移、传输或扩散的便利程度,对流域产沙具有重要影响。为评估基于水文连通性模拟流域输沙量的可行性,以堵河流域为研究对象,分析了1990—2010年流域土壤侵... 详细信息

水文连通性是指泥沙、营养盐等各种物质以水为载体,在空间异质景观或斑块间进行迁移、传输或扩散的便利程度,对流域产沙具有重要影响。为评估基于水文连通性模拟流域输沙量的可行性,以堵河流域为研究对象,分析了1990—2010年流域土壤侵蚀、水文连通性的时空变化特征,并以此估算了流域输沙量。结果表明:1990—2000年流域土壤侵蚀模数与连通程度不断增加,连通性等级主要由低和中低连通性转变为中高和高连通性。2000—2010年流域连通程度和土壤侵蚀模数均呈现不断减小的趋势。此外,输沙量估算值与实测值具有显著的线性关系(R^( 2)=0.92),但由于未考虑水库、湖泊等对泥沙输移的拦截效应,输沙量估算值要高于实测值,后续研究需将其纳入模型以更准确地评估流域输沙量。研究结果对于提高模型的适用性以及理解流域泥沙输移过程具有重要意义。

关键词：土壤侵蚀水文连通性输沙量堵河流域 IC指数

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