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Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton

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Journal of Integrative Plant Biology 2009年第5期51卷 456-465页

作者： hai-hong shang chuan-liang liu chao-jun zhang feng-lian li wei-dong hong fu-guang li Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture Chinese Cotton Research Institute Chinese Agricultural Academy of Sciences Anyang 455004 China

Over the past few decades genetic engineering has been applied to improve cotton breeding. Agrobacterium medicated transformation is nowadays widely used as an efficient approach to introduce exogenous genes into cotton for genetically modified organisms. However, it still needs to be improved for better transformation efficiency and higher embryogenic callus induction ratios. To research further the difference of mechanisms for morphogenesis between embryogenic callus and non-embryogenic callus, we carried out a systematical study on the histological and cellular ultrastructure of Agrobacterium transformed calli. Results showed that the embryogenic callus developed nodule-like structures, which were formed by small, tightly packed, hemispherical cells. The surface of some embryogenic callus was covered with a fibrilar-like structure named extracellular matrix. The cells of embryogenic calli had similar morphological characteristics. Organelles of embryogenic callus cells were located near the nucleus, and chloroplasts degraded to proplastid-like structures with some starch grains. In contrast, the non-embryogenic calli were covered by oval or sphere cells or small clusters of cells. It was observed that cells had vacuolation of cytoplasm and plastids with a well organized endomembrane system. This study aims to understand the mechanisms of embryogenic callus morphogenesis and to improve the efficiency of cotton transformation in future.

关键词： cell organization pattern chloroplasts embryogenic callus Gossypiurn hirsutum histological structure transformation ultrastructure

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai haiying Cai Huacheng Cai Lorenzo Calibbi junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang wei chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen fuqing Chen fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding haiyi dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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Advances in nuclear detection and readout techniques

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Nuclear Science and Techniques 2023年第12期34卷 281-358页

作者： Rui He Xiao‑Yang Niu Yi Wang hong‑wei liang hong‑Bang liu Ye Tian hong‑lin zhang chao‑Jie Zou Zhi‑Yi liu Yun‑Long zhang hai‑Bo Yang Ju Huang hong‑Kai Wang wei‑Jia Han Bei Cao Gang Chen Cong Dai li‑Min Duan Rui‑Rui Fan Fang‑Fa fu Jian‑Hua Guo dong Han wei Jiang Xian‑Qin li Xin li Zhuo‑Dai li Yu‑Tie liang Shun liao De‑Xu lin Cheng‑Ming liu Guo‑Rui liu jun‑Tao liu Ze Long Meng‑Chen Niu Hao Qiu Hu Ran Xiang‑Ming Sun Bo‑Tan Wang Jia Wang Jin‑Xiang Wang Qi‑lin Wang Yong‑Sheng Wang Xiao‑chuan Xia Hao‑Qing Xie He‑Run Yang hong Yin hong Yuan Chun‑Hui zhang Rui‑guang Zhao Ran Zheng Cheng‑Xin Zhao Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China Advanced Energy Science and Technology guangdong Laboratory Huizhou 516000China Key Laboratory of Particle and Radiation Imaging Department of Engineering PhysicsTsinghua UniversityBeijing 100084China School of Integrated Circuits Dalian University of TechnologyDalian 116020China guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Electronic Engineering School Heilongjiang UniversityHarbin 150080China Spallation Neutron Source Science Center Dongguan 523803China Micro‑electronic Department Harbin Institute of TechnologyHarbin 150001China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210034China liaoning Academy of Materials Shenyang 110167China Istituto Nazionale di Fisica Nucleare Sezione di PerugiaPerugiaItaly School of Mathematics and Physics Chengdu University of TechnologyChengdu 620035China PLAC Key Laboratory of Quark and Lepton Physics(MOE)Central China Normal UniversityWuhan 430079China School of Electronics and Information Northwestern Polytechnical UniversityXi’an 710129China State Key Lab of Superhard Materials College of PhysicsJilin UniversityChangchun 130012China School of Computer Science Northwestern Polytechnical UniversityXi’an 710129China

“A Craftsman Must Sharpen His Tools to Do His Job,”said *** detection and readout techniques are the foundation of particle physics,nuclear physics,and particle astrophysics to reveal the nature of the ***,they are being increasingly used in other disciplines like nuclear power generation,life sciences,environmental sciences,medical sciences,*** article reviews the short history,recent development,and trend of nuclear detection and readout techniques,covering Semiconductor Detector,Gaseous Detector,Scintillation Detector,Cherenkov Detector,Transition Radiation Detector,and Readout *** explaining the principle and using examples,we hope to help the interested reader underst and this research field and bring exciting information to the community.

关键词： Silicon detector Wide bandgap detector Time projection chamber(TPC) Multigap resistive plate chamber detector(MRPC) Scintillation detector Calorimeters Cherenkov detector Transition radiation detector Artificial intelligence Trigger-less readout

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棉花体细胞胚发生机理的研究进展

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西北植物学报 2009年第3期29卷 637-642页

作者：商海红刘传亮张朝军武芝霞李付广中国农业科学院棉花研究所农业部棉花遗传改良重点开放实验室河南安阳455004

棉花是一种较难通过体细胞胚发生完成植株再生的作物,而体细胞胚发生是限制棉花基因工程和细胞工程得以广泛应用的主要因素.研究者从不同角度探讨了棉花体细胞胚发生机理并取得了很大的进展,为棉花的遗传转化提供了重要的技术支持,同时... 详细信息

棉花是一种较难通过体细胞胚发生完成植株再生的作物,而体细胞胚发生是限制棉花基因工程和细胞工程得以广泛应用的主要因素.研究者从不同角度探讨了棉花体细胞胚发生机理并取得了很大的进展,为棉花的遗传转化提供了重要的技术支持,同时成功建立了一些棉花植株再生体系.本文分别从基因型限制、胚胎学、生理生化变化、分子机理等方面阐述了近年来棉花体细胞发生机制的研究进展,并讨论了本研究领域目前存在的主要问题及相应对策.

关键词：陆地棉体细胞胚发生畸形体细胞胚机理

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陆地棉体细胞胚胎发生过程中的细胞外基质研究(英文)

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棉花学报 2012年第2期24卷 167-175页

作者：商海红袁有禄刘传亮张朝军武芝霞张雪妍李付广中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455004

研究棉花体细胞胚胎发生对于促进棉花基因工程育种具有重要意义。利用扫描电镜和透射电镜对陆地棉体细胞胚胎发生过程的研究发现,细胞外基质在棉花体细胞胚胎发生的不同阶段呈现出有规律的动态变化。当普通愈伤组织细胞发育成胚性愈伤... 详细信息

研究棉花体细胞胚胎发生对于促进棉花基因工程育种具有重要意义。利用扫描电镜和透射电镜对陆地棉体细胞胚胎发生过程的研究发现,细胞外基质在棉花体细胞胚胎发生的不同阶段呈现出有规律的动态变化。当普通愈伤组织细胞发育成胚性愈伤组织时,细胞外基质就会出现;随着原胚的形成,细胞外基质就会形成发达的网络状结构;随着胚状体的进一步形成和发育,细胞外基质则逐渐降解。在愈伤组织和非胚性愈伤组织中,始终未发现细胞外基质。结果表明,细胞外基质与体细胞胚胎发生能力关系密切,是原胚形成的重要标记。

关键词：棉花体细胞胚胎发生细胞外基质扫描电镜透射电镜

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Main Achievements of Cotton Large-scale Transformation System

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棉花学报 2008年第S1期20卷 80-页

作者： li fu-guang,liu chuan-liang,WU Zhi-xia,zhang chao-jun,zhang Xue-yan(Cotton Research Institute,Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China) Cotton Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement Ministry of AgricultureAnvangHenan 455000China

Cotton large-scale transformation methods system was established based on innovation of cotton transformation *** obtains 8000 transgenic cotton plants per year by combining Agrobacterium tumefaciens-mediated,pollen-t... 详细信息

关键词： cotton transformation GMO mutant

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亚洲棉石系亚1号耐旱相关基因SSH文库的构建及其分析

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棉花学报 2010年第2期22卷 110-114页

作者：张玲李付广刘传亮张朝军武芝霞中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室河南安阳455000

通过抑制消减杂交技术成功地构建了亚洲棉石系亚1号耐旱相关基因cDNA文库。通过蓝白斑筛选、菌落PCR及生物信息学等分子生物学手段分析和检验了库的质量,最终筛选出阳性克隆392个。通过EST测序得到265个单一序列,其中41个为重叠群,224... 详细信息

通过抑制消减杂交技术成功地构建了亚洲棉石系亚1号耐旱相关基因cDNA文库。通过蓝白斑筛选、菌落PCR及生物信息学等分子生物学手段分析和检验了库的质量,最终筛选出阳性克隆392个。通过EST测序得到265个单一序列,其中41个为重叠群,224个为单拷贝。生物信息学分析结果表明,该文库含有大量与耐旱相关的基因,并且涉及植物生理中多种代谢途径。

关键词：亚洲棉干旱胁迫抑制性消减杂交

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Consensus statement on layout and delivery of automatic external defibrillator in China

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Journal of hainan Medical University 2020年第15期26卷 18-23页

作者： Yu Cao Song Chen Yan-Fen Chai Xiao-Hui Chen Yu-Guo Chen Shao-Wen Cheng Wen-wei Cheng Bang-Han Ding Yang Deng Jie fu Bang-Jiang Fang Mai-Ying Fan li Gui Ping Gong wei Guo Shu-Bin Guo liang Huang Ji-Yuan Han Zhong Ji Xian-Fei Ji Xin-Cai Ji hong-Xu Jin Xin-jun Jiang Wang-Xiang Jiang Jia-liang liu Xiao-Ran liu Zhi liu chuan-Zhu Lv feng Lu Tan-Shi li Xin li Yong li shang-Lun li Shuang-Ming li Xiao-Gang li Bao-jun li Pei-Wu li Chun-Sheng li Zhi-jun li Chang-Luo li Xiang-Min li Yin-Ping li Zong-jun Lu Zu-Xun Lu Zhong-Qiu Lu Zhi-Qian Luo Xiao-Xing liao Jian-Bo Lei Tao Ma Yu Ma Yue-feng Ma Qing-Bian Ma lin-Hao Ma jun-Qi Ma Shi-Nan Nie Jie-Miao Ou-Yang Peng Peng Qiao Pei Shu-Ming Pan Guo-Zhong Qing li-Jie Qin Wu-Ying Qiao Fei Shao wei Song Xing-Yue Song Zhen-Ju Song Rong-Ju Sun De-Ya shang Du-Juan Sha Gui-Lan Tao Guo-Gang Tian Ying-Ping Tian Jian-guang Tian chao-Yang Tong Zi-Ren Tang Meng-Xiong Tang Guo-Ping Wu Zhi Wan Tao Wang Xin Wang Peng Wang Zhong Wang Tong Wang Ri-Xing Wang Yi-Xin Wang Xiao-Dan Wang ling Wu wei wei Hua Xiang feng Xu jun Xu Jie Xu Miao-Rong Xie Shuo-Gui Xu Tie Xu feng Yu Wen Yi li-Shan Yang Shi-Jiao Yan Jin-Jian Yao Shou-Chun Yan Xue-Zhong Yu Xian-Zhi Ye Xian-liang Yan fu-Bing Zhou Rong-Bin Zhou Ming Zhou Qiang Zhou Jin Zhou Ping Zhou Chang-Ju Zhu Hua-dong Zhu Ji-hong Zhu hong zhang Mao zhang Hua zhang Na zhang Guo-Qiang zhang Guo-Xiu zhang Jin-Song zhang Wen-Wu zhang Wen-Zhong zhang Jian-feng zhang Yi-Min Zhu jun Zeng Yuan Zheng Zhi-Jie Zheng Shen Zhao Yan Zhao Min Zhao Bin Zhao Xiao-dong Zhao Xiao-Jing Zhao hong-Ke Zeng Sheng-Qiang Zou The Chinese Society of Emergency Medicine Research Unit of Island Emergency Medicine Chinese Academy of Medical SciencesHainan Medical University Key Laboratory of Emergency and Trauma of Ministry of Education Hainan Medical UniversityHaikouHainanChina Emergency and Trauma College Hainan Medical UniversityHaikouHainanChina Department of Emergency Hainan Clinical Research Center for Acute and Critical DiseasesThe Second Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina Key Laboratory of hainan Trauma and Disaster Rescue Key Laboratory of Trauma of hainan Medical University

*** Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac *** is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].

关键词： cardiac death respiratory

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海岛棉转录因子EREB5基因的克隆及特征研究

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棉花学报 2011年第3期23卷 205-211页

作者：刘坤单国芳李付广刘传亮张朝军张雪妍武芝侠中国农业科学院棉花研究所/农业部棉花遗传改良重点开放实验室河南安阳455000

近年来,ERF(Ethylene-responsive element binding factor)家族转录因子已成为植物抗逆、抗病的分子机制和作物分子育种研究的热点。本研究以电子克隆、同源扩增和RACE(Rapid-amplification of cDNA ends)技术相结合的方法从海岛棉中分... 详细信息

近年来,ERF(Ethylene-responsive element binding factor)家族转录因子已成为植物抗逆、抗病的分子机制和作物分子育种研究的热点。本研究以电子克隆、同源扩增和RACE(Rapid-amplification of cDNA ends)技术相结合的方法从海岛棉中分离出了一条新的ERF族转录因子基因,命名为EREB5。该基因包含一个573 bp长的开放阅读框,预期编码190 aa长的蛋白质分子。序列分析表明该蛋白除含有一个ERF保守域外,还含有一段核定位信号序列、一段富丝氨酸的激活域、多种磷酸化位点和一个土豆抑制子Ⅰ型家族基序等。构建系统发育树分析表明该因子属于ERF亚家族B3亚组。瞬时表达实验证明该因子定位于细胞核内,同时,凝胶阻滞实验结果说明EREB5蛋白和GCC盒具有较强的结合能力。再者,荧光定量PCR结果表明乙烯和黄萎病菌处理可以诱导该基因的表达。这些结果暗示EREB5蛋白很可能在黄萎病抗性机制中扮演者重要角色。

关键词：海岛棉 ERF 黄萎病 EREB5 基因克隆

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海岛棉ERF族转录因子EREB6基因克隆及特征分析

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河南师范大学学报（自然科学版） 2011年第2期39卷 106-110页

作者：刘坤单国芳李付广刘传亮张朝军张雪妍武芝侠中国农业科学院棉花研究所农业部棉花遗传改良重点开放实验室河南安阳455004

以电子克隆,同源扩增和RACE技术相结合的方法从海岛棉中克隆出了一条新基因,命名为EREB6,它包含一个669 bp长的开放阅读框,预期编码222个氨基酸长的蛋白质.序列分析发现预期蛋白含有一段核定位信号序列,GFP融合蛋白瞬时表达实验证明该... 详细信息

以电子克隆,同源扩增和RACE技术相结合的方法从海岛棉中克隆出了一条新基因,命名为EREB6,它包含一个669 bp长的开放阅读框,预期编码222个氨基酸长的蛋白质.序列分析发现预期蛋白含有一段核定位信号序列,GFP融合蛋白瞬时表达实验证明该蛋白定位于细胞核;含有一个保守的ERF域,属于ERF族;同源分析表明该转录因子和AtERF13,Pti4和TiERF1同属于B3a小组,同源性很高,暗示它们具有相似的功能.

关键词：转录因子 ERF 海岛棉 EREB6

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