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Construction and Expression of Human Survivin and Preparation of Its Polyclonal Antibody

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Chemical Research in Chinese Universities 2008年第6期24卷 767-770页

作者： ZHANG hai-hong ZHANG Xi-zhen ZHAO Dong-hai SHI He-liang YU Yong-hui wu Yong-ge YU Xiang-hui KONG Wei Vaccine Research Center College of Life Science Jilin University Changchun 130012 P R. China

Survivin, a novel member of inhibitor of apoptosis（IAP） protein family, is aberrantly expressed in cancer but undetectable in normal, differentiated adult tissues. The cancer-specific expression of survivin, coupled with its importance in inhibiting cell death and in regulating cell division makes it a useful diagnostic marker of cancer and a potential target for cancer treatment. Survivin cDNA amplified from the total RNA of 293 cells through RT-PCR was cloned into prokaryotic expression vector pRSET-B. The recombinant plasmid pRSET-B-Surv was expressed in *** BL21, and the relative molecule mass（Mr） of expressed fusion protein was approximately 21000. The recombinant protein was purified through Ni^2＋ affinity chromatography column and characterized by SDS-PAge and Western blot. The purified recombinant protein was then injected into rabbits, and antisurvivin polyclonal antibody with a high titer was obtained.

关键词： Clone Survivin Expression Polyclonal antibody

Professor Ya-Ling Han

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Journal of geriatric Cardiology 2013年第2期10卷 121-122页

作者： hai-Yun wu Institute of geriatric Cardiology Chinese PLA General Hospital28 Fuxing Road100853 BeijingChina

Professor Ya-Ling Han (Figure 1), a famous cardiologist in China, was born in wuhan, Hubei province, in *** graduated from Harbin Medical University in *** getting her doctorate in 1994 from Shanghai Second Military Medical University, professor Han worked as the director and professor of the Department of *** then worked as the vice president at Shenyang Northern Hospital since *** the past two decades, she has been persistently leading the development of percutaneous coronary intervention (PCI) technique for complex coronary lesions and high risk *** 1998 to 1999 she received PCI Fellow training in Singapore National Heart Center for professional enhancement.

关键词： famous cardiologist doctorate professional

Bioinspired Passive Tactile Sensors Enabled by Reversible Polarization of Conjugated Polymers

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Nano-Micro Letters 2025年第1期17卷 361-377页

作者： Feng He Sitong Chen Ruili Zhou Hanyu Diao Yangyang Han Xiaodong wu School of Mechanical Engineering Sichuan UniversityChengdu 610065People’s Republic of China School of Software Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065People’s Republic of China State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengdu 610065People’s Republic of China

Tactile perception plays a vital role for the human body and is also highly desired for smart prosthesis and advanced *** to active sensing devices,passive piezoelectric and triboelectric tactile sensors consume less power,but lack the capability to resolve static ***,we address this issue by utilizing the unique polarization chemistry of conjugated polymers for the first time and propose a new type of bioinspired,passive,and bio-friendly tactile sensors for resolving both static and dynamic ***,to emulate the polarization process of natural sensory cells,conjugated polymers(including poly(3,4-ethylenedioxythiophen e):poly(styrenesulfonate),polyaniline,or polypyrrole)are controllably polarized into two opposite states to create artificial potential *** controllable and reversible polarization process of the conjugated polymers is fully in situ ***,a micro-structured ionic electrolyte is employed to imitate the natural ion channels and to encode external touch stimulations into the variation in potential difference *** with the currently existing tactile sensing devices,the developed tactile sensors feature distinct characteristics including fully organic composition,high sensitivity(up to 773 mV N^(−1)),ultralow power consumption(nW),as well as superior *** demonstrations,both single point tactile perception(surface texture perception and material property perception)and two-dimensional tactile recognitions(shape or profile perception)with high accuracy are successfully realized using self-defined machine learning *** tactile sensing concept innovation based on the polarization chemistry of conjugated polymers opens up a new path to create robotic tactile sensors and prosthetic electronic skins.

关键词： Passive tactile sensors Reversible polarization of conjugated polymers Tactile perception Machine learning algorithm Object recognition

Analysis of genetic Effects for Heterosis of Erucic Acid and Glucosinolate Contents in Rapeseed (Brassica napus L.)

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Agricultural Sciences in China 2011年第10期10卷 1525-1531页

作者： ZHANG hai-zhen SHI Chun-hai wu Jian-guo Department of Agronomy College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 P.R.China Key Laboratory of genetic and Breeding for Landscape Plant Hangzhou Botanical Garden Hangzhou 310012. P.R. China

The embryo, cytoplasmic, and maternal heterosis for erucic acid content （EAC） and glucosinolate content （GLS） of rapeseed （Brassica napus L.） were studied by using the genetic models for quantitative traits of seeds in diploid crops. Eight parents were included in a diallel mating design in two years. It was found that the heterosis of EAC and GLS was simultaneously controlled by genetic main effects and genotype×environment （ge） interaction effects. The general heterosis of most crosses for EAC was significantly positive, while it was not for GLS. The general heterosis was more important for two quality traits of rapeseed because of the low ge interaction heterosis in both years, especially for GLS. Among different genetic systems, significant positive embryo general heterosis and the negative maternal general heterosis were found for EAC and GLS in most hybrid crosses. Some hybrids with significant negative interaction heterosis were detected for either of EAC or GLS. In general, maternal general and interaction heterosis was more important for reducing EAC and GLS of rapeseed.

关键词： rapeseed quality general heterosis ge interaction heterosis

Ion-implanted Mechanism of the Deposition Process for Diamond-Like Carbon Films

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Chinese Physics Letters 2011年第1期28卷 149-152页

作者： WANG Xue-Min wu Wei-Dong WANG Yu-Ying WANG hai-Ping ge Fang-Fang TANG Yong-Jian JU Xin Research Center of Laser Fusion China Academy of Engineering Physics Mianyang 621900 Department of Physics University of Science-and Technology BeijingBeijing 100083

Due to the local densification, high-energy C and doped ions can greatly affect the bonding configurations of diamond-like carbon films. We investigate the corresponding affection of different incident ions with energy from 10eV to 600eV by Monte Carlo methods. The ion-implanted mechanism called the subplantation （for C, N, O, W, Y, etc.） is confirmed. Obvious thermal effect could be induced by the subplantation of the incident ions. Further, the subplantation of C ions is proved by in situ reflection high energy electron diffraction （RHEED）. The observation from an atomic force microscope （AFM） indicates that the initial implantation of C ions might result in the final primitive-cell-like morphology of the smooth film （in an area of 1.2 mm× 0.9 mm, rms roughness smaller than 20 nm by Wyko）.

关键词： Surfaces interfaces and thin films

SepFE:Separable Fusion Enhanced Network for Retinal Vessel Segmentation

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Computer Modeling in Engineering & Sciences 2023年第9期136卷 2465-2485页

作者： Yun wu ge Jiao Jiahao Liu College of Computer Science and Technology Hengyang Normal UniversityHengyang421002China Hunan Provincial Key Laboratory of Intelligent Information Processing and Application Hengyang421002China

The accurate and automatic segmentation of retinal vessels fromfundus images is critical for the early diagnosis and prevention ofmany eye diseases,such as diabetic retinopathy(DR).Existing retinal vessel segmentation approaches based on convolutional neural networks(CNNs)have achieved remarkable ***,we extend a retinal vessel segmentation model with low complexity and high performance based on U-Net,which is one of the most popular *** view of the excellent work of depth-wise separable convolution,we introduce it to replace the standard convolutional *** complexity of the proposed model is reduced by decreasing the number of parameters and calculations required for *** ensure performance while lowering redundant parameters,we integrate the pre-trained MobileNet V2 into the ***,a feature fusion residual module(FFRM)is designed to facilitate complementary strengths by enhancing the effective fusion between adjacent levels,which alleviates extraneous clutter introduced by direct ***,we provide detailed comparisons between the proposed SepFE and U-Net in three retinal image mainstream datasets(DRIVE,STARE,and CHASEDB1).The results show that the number of SepFE parameters is only 3%of U-Net,the Flops are only 8%of U-Net,and better segmentation performance is *** superiority of SepFE is further demonstrated through comparisons with other advanced methods.

关键词： Retinal vessel segmentation U-Net depth-wise separable convolution feature fusion

An experimental investigation of dual-resonant and non-resonant responses for vortex-induced vibration of a long slender cylinder

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Science China(Physics,Mechanics & Astronomy) 2014年第2期57卷 321-329页

作者： wu XiaoDong ge Fei HONG YouShi College of Mechanics Taiyuan University of Technology State Key Laboratory of Nonlinear Mechanics Institute of MechanicsChinese Academy of Sciences

Experimental results of the dual-resonant and non-resonant responses are presented for vortex-induced vibrations(VIV)of a long slender *** cylinder has a diameter of 10mm and a length of 3.31 m,giving an aspect ratio of *** cylinder was towed by a carriage with the velocity up to 1.5 m/s,with the Reynolds number varying from 2500 to *** different weights were used to provide the initial *** resonance means that resonance occurs simultaneously in both the cross-flow(CF)and in-line(IL)*** experiments were conducted in two *** the first stage,dual-resonant dynamic features of the cylinder subjected to vortex-induced excitation were *** features of CF and IL vibration amplitude,motion orbits,phase angle differences,dominant frequencies and mode order numbers are *** the second stage of the experiments,particular emphasis was placed on non-resonant dynamic *** variation of multi-mode modal displacement amplitudes was investigated in detail.

关键词： vortex-induced vibrations long slender cylinder dual-resonant responses non-resonant responses

A facile synthesis of 2-aryloxypyrimidine derivatives via a tandem reductive amination/intermolecular S_NAr sequence

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第2期11卷 94-101页

作者： hai-feng wu Pei-zhi ZHANG Jun wu Department of Chemistry Zhejiang University Hangzhou 310027 China School of Biological and Chemical Engineering Zhejiang University of Science and Technology Hangzhou 310012 China

A novel tandem reductive amination/intermolecular nucleophilic aromatic substitution (SNAr) sequence has been established for the synthesis of amine containing pyrimidine in formation of one carbon-oxygen and one carbon-nitrogen bonds in a one-pot fashion. Treatment of aldehyde with arylamine, 2-methanesulfonyl-4,6-dimeth-oxypyrimidine and sodium borohydride provides good overall yield. The p-toluenesulfonic acid (PTSA) can be used as activator and is generally needed in the reaction. Dioxane is the preferred reaction solvent, but reactions can also be carried out in tetrahydrofuran (THF), MeCN, toluene and dichloromethane. The procedure is carried out effectively in the presence of K2CO3. The reaction proceeds smoothly with aromatic aldehydes and arylamines possessing elec-tron-donating or-withdrawing groups. This method can be applied to the synthesis of the oilseed rape herbicide and is superior to the classical one in several aspects: cutting out several purification steps, minimizing solvent use and chemical waste, and saving time. Its advantages such as operational convenience, high-efficient synthesis, and starting material availability make it a desirable method for preparing amines with molecular diversity and biological activity.

关键词： Reductive amination/intermolecular SNAr C-O and C-N bonds Amine Pyrimidine Herbicide

Copper-catalyzed decarboxylative Se insertion coupling of indoles and propiolic acids

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Chinese Chemical Letters 2022年第10期33卷 4531-4535页

作者： ge wu Xueying Zhou Caihong Wang School of Pharmaceutical Sciences Wenzhou Medical UniversityWenzhou 325035China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

A novel and efficient copper-catalyzed decarboxylative alkynylselenation of indoles with Se powder and propiolic acids has been *** outstanding advantages of this protocol not only nicely avoid the use of prefabricated arylselenation reagent and address the facile over-selention issues,but also enrich the chemistry of selenium ***,this reaction could be extended to pyrrole,and the practical utility of this transformation has been demonstrated in gram-scale synthesis and late-stage indolylselenation of Clofibrate-derived propiolic acid.

关键词： Se powder Copper catalysis Decarboxylation Multi-component reaction Propiolic acids